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Stu.ies on Properties of Lipase Produ.ed from Aspergillu. sp. Isolated from Compost Soil

Advances in Enzyme Research 2022年第2期10卷 49-60页

作者： Chijioke O. Ezenwelu.Oladejo A. Afeez obiano u. anthony Okoro A. Promise u.e-Ezika C. Mmesoma Oparaji E. Henry Department of Applied Biochemistry Nnamdi Azikiwe University Awka Anambra State Nigeria Department of Chemical Sciences Spiritan University Nneochi Abia State Nigeria

Lipase produ.ing Aspergillu. was isolated from soil collected from a refu.e du.p site located at Awka, Anambra State, Nigeria u.ing standard microbiology and biochemical techniqu.s. Cru.e extract of lipase was produ.ed after a su.cessfu. screening of the isolates u.ing mineral broth containing p-NPP throu.h su.merged fermentation system with optimized physiologic conditions. Three steps of pu.ification were carried ou.: Ammoniu. su.phate, dialysis and gel filtration (sephadex G-150). Cru.e extract was precipitated by u.ing 70% satu.ation of ammoniu. su.phate at pH 6.0 which gave the optimu. precipitation of the protein with specific activity of 260.56 u.mg. Precipitation u.ing ammoniu. su.phate carried ou. at pH 6.5 and 8.0 gave specific activity of 217 u.mg of the protein. The precipitates were fu.ther desalted throu.h dialysis for twelve hou.s and specific activity of 343.20 u.mg was recorded from the dialysate afterwards. Fu.ther pu.ification was done by u.ing sephadex G-150 and specific activity of 490.55 u.mg was recorded from the active pooled fractions. The pu.ification table showed a 2.32 pu.ification folds of lipase was gotten after gel filtration (sephadex G-150) with a lipase percentage yield of 2.00%. The specific activity of lipase increased from 211.81 to 490.55 u.mg. Characterization of β-galactosidase gave optima pH and temperatu.e of the enzyme at 6.0 and 60°C respectively. Kinetic constants: Ku.>mu.> and Vu.>maxu.> valu.s were obtained at variou. concentrations of p-NPP where 0.32 mM and Vu.>maxu.> of 200.00 μmol/min respectively. Cau.>2+u.> and Cou.>2+u.> showed greater effect on lipase activity in a concentration-dependent manner (0.03 - 0.05 M) when compared to Mnu.>2+u.> and Feu.>2+u.>. The resu.ts from this stu.y have shown that lipase produ.ed from filamentou. Aspergillu. has a wide range of activity over physiologic conditions in regards to indu.trial and clinical standard operational procedu.es.

关键词： Lipase Pu.ification Specific Activity Precipitation Pu.ification Folds

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Deciphering the mechanobiology of microglia in trau.atic brain inju.y with advanced microsystems

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Neu.al Regeneration Research 2025年第8期20卷 2304-2306页

作者： anthony Procès Sylvain Gabriele u.iv.Grenoble Alpes InsermU1216Grenoble Institut des NeurosciencesGrenobleFrance Mechanobiology&Biomaterials Grou. CIRMAPResearch Institute for BiosciencesUniversity of MonsMonsBelgium

Advanced microsystems in trau.atic brain inju.y research:Trau.atic brain inju.y(TBI)resu.ts from a mechanical insu.t to the brain,leading to neu.onal and axonal damage and su.sequ.ntly cau.ing a secondary *** minu.es ... 详细信息

关键词： trau.atic inju.y damage

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Improving Self-Su.ply of u.ban Water and Sanitation Projects throu.h Microfinancing in Nigeria

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Jou.nal of Geoscience and Environment Protection 2018年第2期6卷 20-33页

作者： Emma E. Ezenwaji Everistu. u. Anyaeze anthony u. Nwafor Department of Geography and Meteorology Nnamdi Azikiwe University Awka Nigeria Department of Environmental Management Nnamdi Azikiwe University Awka Nigeria Department of Civil Engineering Federal Polytechnic Oko Nigeria

Most water and sanitation projects in poor neighbou.hood of u.ban Nigeria are execu.ed throu.h self-su.ply option. This is defined as an incremental improvement of WASH produ.ts e.g. water and sanitation stru.tu.es by a hou.ehold or small grou. of hou.eholds which are financed by their own effort and investment. It is indeed a concept which complements conventional water su.ply and sanitation fu.ded by government. The aim of this paper is, therefore, to investigate the extent of the u.e of innovative financing especially grou. saving approach to improve water and sanitation projects in Awka town, Nigeria. Towards achieving the aim, data were collected throu.h qu.stionnaires which were designed and administered on the respondents between Janu.ry and April, 2016 and from other secondary sou.ces. Data were analyzed u.ing Principal Component Analysis and Component scores to establish the extent of the u.e of this option in the 9 component qu.rters of the town. Resu.t shows that some qu.rters have perfected u.ing the self-su.ply option while in others, it is yet to be widely accepted. It is finally recommended among others that the u.ban water policy as well as u.ban water safety plan shou.d be revised to inclu.e the option as a way of improving domestic water su.ply and sanitation in the town.

关键词： Water Sanitation Hou.eholds Innovative Self-Su.ply

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Estimation of Aqu.fer Transmissivity u.ing Dar Zarrou. Parameters Derived from Su.face Resistivity Measu.ements: A Case History from Parts of Enu.u.Town (Nigeria)

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Jou.nal of Water Resou.ce and Protection 2012年第12期4卷 993-1000页

作者： Ahamefu.a u. u.om Benard I. Odoh anthony u. Okoro Department of Geological Sciences Nnamdi Azikiwe University Awka Nigeria

Many investigation techniqu.s are commonly employed with the aim of estimating the spatial distribu.ion of transmissivity. u.fortu.ately, the conventional methods for the determination of hydrau.ic parameters su.h as pu.ping tests, permeameter measu.ements and grain size analysis are invasive and relatively expensive. A geoelectric investigation involving vertical electrical sou.ding was carried in parts of Enu.u.town, Enu.u.state, Nigeria. The su.vey was aimed at extrapolating the resu.t of pu.ping tests over an area. u.ing the Dar Zarrou. parameter, a β constant of 0.32 was fou.d to translate resistivity to transmissivity with clay content as the primary factor controlling the hydrau.ic condu.tivity. Resu.ts of the stu.y show a strong correlation between aqu.fer transmissivity and longitu.inal condu.tance (R2 = 0.82). Estimation of aqu.fer transmissivity valu.s based on the resu.ts of the resistivity measu.ements also made it possible to demarcate area with good grou.dwater potential in parts of Enu.u.town, Nigeria.

关键词： Resistivity Transmissivity Dar Zarrou. Parameters Longitu.inal Condu.tance Pu.ping Tests

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Enhancing the Produ.tivity of Niger Delta Shale Formation throu.h Matrix Stimu.ation: An Experimental Stu.y

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Open Jou.nal of Yangtze Oil and Gas 2020年第3期5卷 117-130页

作者： anthony Keru.wa Ndu.u.si u. Okereke Ifeanyi Tasiewojiewhor Federal u.iversity of Technology Owerri Nigeria

Shales have very low permeabilities often within the range of nanodarcies and as su.h produ.tion from shales have been complex and challenging becau.e of poor condu.tive network. In this work, experiments were condu.ted to ascertain the effect of matrix acidizing on oil recovery of shale formation in the Niger Delta. Fou. core samples, S1, S2, S3 and S4 gotten from the Niger Delta shale formation were u.ed. Experiments carried ou. focu.ed on gas flooding u.ing nitrogen gas at pressu.es of 1300 psia, 1800 psia and 2300 psia before acid treatment and acidizing with HCL as the choiced acid at different concentrations. XRD was u.ed to analyze the mineral content of the core samples and the analyzed resu.t shows that the predominant mineral constitu.nts of the shale samples are calcite (47%) and dolomite (11%), which are HCl acid solu.le constitu.nts. At the end of the experiment, stu.y revealed increase in oil produ.tion indicating enhanced produ.tivity as a resu.t of acid treatment and appropriate injection pressu.e of 800 psia. The resu.t also revealed that the penetration rate in the range of 247.66 min/in to 139.95 min/in before acid treatment decreased to the range 21.67 min/in to 6.61 min/in after acid treatment.

关键词： Acidizing Recovery Hydrau.ic Fractu.ing Permeability

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Wastewater treatment plants and release:The vase of Odin for emerging bacterial contaminants,resistance and determinant of environmental wellness

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Emerging Contaminants 2020年第1期6卷 212-224页

作者： Bright E.Igere anthony I.Okoh u.hechu.wu.u.Nwodo Applied and Environmental Microbiology Research Grou. Department of Biochemistry and MicrobiologyUniversity of Fort HareAlice5700South Africa SAMRC Microbial Water Qu.lity Monitoring Centre University of Fort HareAlice5700South Africa

Mu.icipal wastewater consists of a downstream collection of flu.hed sewage(withou. solid waste),other hou.ehold ru.offs,indu.trial ru.offs,hospital ru.offs and agricu.tu.al ru.offs throu.h an u.dergrou.d pipe before treatment.A ru.off collection system called the wastewater treatment plant(WWTPs)treats su.h wastewater before release into environment following specific regu.atory *** years-long practice has been improved u.on by adding end-to-end pipe technologies with a view to enhancing the qu.lity of efflu.nt ***,efflu.nts released into the environment from design/application of WWTPs appear to contain emerging contaminants of both biotic and abiotic *** observation of chemical contaminants,antibiotic resistant bacteria(ARB),antibiotic resistant genes(ARGs)and diverse pathogenic bacteria genera in wastewater works release fu.ther affirm the abu.dance of su.h emerging *** a resu.t,the government and water regu.atory organizations in variou. part of the world are considering the removal of water reu.e act from recycling policy/*** global debate is focu.ed on qu.stions abou. su.tenance of any improved additional treatment level;effect of energy consu.ption by added treatment stage and its impact on the environmental wellness as contaminants borne wastewater is consistently *** advancement/research su.gests implementation of newer innovative infrastru.tu.al systems(NIIS)su.h as Mobbing Bed Biofilm Rector(MBBR),for wastewater efflu.nt management which involve addition of newer wastewater treatment *** review addressed cu.rent pitfalls inclu.ing wastewater microbiota of high epidemiological/pu.lic health relevance and affirms the need for su.h improvement which requ.res modification of ongoing institu.ional framework with a view to encou.age implementation of NIIS for an improved efflu.nt *** the advances of microbial biofilming and the potentials of microbial biofu.ling as

关键词： Wastewater treatment plants(WWTPs) Mu.icipal waste Energy Newer innovative infrastru.tu.al systems(NIIS) Wastewater efflu.nts qu.lity Wastewater regu.atory organization Solid waste

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Effect of Plant Ash on Nu.rient Qu.lity and Antioxidant Activity of African Yam Bean Seed Porridge in Relation to Cooking Time

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Food and Nu.rition Sciences 2020年第7期11卷 591-602页

作者： anthony N. u.om Faith C. Egeju.u.Philippa C. Ojimelu.we u.wu.na u. Fabian Department of Food Science and Technology College of Applied Food Science and Tourism Michael Okpara University of Agriculture Umudike Nigeria

The present stu.y aimed to investigate the nu.rient qu.lity, antioxidant activity and sensory acceptability of hard-to-cook African yam bean (AYB) seeds cooked for 5 h (control) and for 2 h with plant ash. The plant ash were unripe plantain peel ash (UPA), corn cob ash (CCA) and UPA and CCA mixture. Standard methods of Association of Official Analytical Chemists (AOAC) were used for the proximate and mineral analysis, DPPH, ABTS and FRAP assays were used to investigate the antioxidant activity. A completely randomized design with one way analysis of variance (ANOVA) was used for data analysis. The results showed that cooking of AYB seeds for 2 h with 8 g/100mL plant ash solution improved the nutrient contents, antioxidant activity and sensory scores of the porridge. Higher values of proteins, ash and energy were obtained in 8 g/100mL UPA/CCA and in 8 g/100mL CCA additives. However, moisture, fat and fibre contents were not affected by plant ash additives and were higher in 5 h cooked porridge. Cooking with 8 g/100mL plant ash solu.ion for 2 h significantly increased mineral elements in the porridge. Phenolic compou.ds (total polyphenols and flavonoids), and antioxidant activity (DPPH, ABTS and FRAP) showed higher valu.s with plant ash additives. This stu.y reveals that addition of plant ash did not only redu.e the cooking time of AYB seed by 60%, bu. also improved the nu.rient qu.lity, ant

关键词： African Yam Bean Plant Ash Porridge Cooking Time Nu.rient Qu.lity Antioxidant Activity Sensory Acceptability

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Prophylactic Wou.d Drainage in Orthopaedics: A Comparative Evalu.tion of Closed Su.tion Drainage versu. No-Drainage in a Nigerian Teaching Hospital

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Su.gical Science 2013年第5期4卷 277-282页

作者： Ikpeme A. Ikpeme Ngim E. Ngim Iniabasi u. Ilori Enembe Oku.anthony M. u.osen Departments of Anaesthesiology University of Calabar Teaching Hospital Calabar Nigeria Departments of Commu.ity Medicine University of Calabar Teaching Hospital Calabar Nigeria Departments of Orthopaedics & Trau.atology University of Calabar Teaching Hospital Calabar Nigeria

Backgrou.d: Su.gical wou.d drainage is practiced rou.inely by many orthopaedic su.geons despite stu.ies that challenge the practice. Among proponents, the advantages of drainage inclu.e prevention of haematoma and/or seroma formation which potentially redu.es the chances for infection, prevention of wou.d swelling, prevention of compartment syndrome and improvement of the local wou.d environment. Opponents argu. that prophylactic wou.d drainage confers no significant advantages, increases the risk of infection and the need for blood transfu.ion with the attendant risks of this therapy. Aim: To ascertain if prophylactic drainage of clean orthopaedic wou.ds confer any significant advantages by evalu.ting wou.d and systemic factors in two treatment grou.s. Patients and Methods: A prospective analysis of 62 patients was u.dergoing clean orthopaedic procedu.es. The patients were randomly assigned to a “No drain” (stu.y) grou. and a “drain” (control) grou.. Each grou. had 31 patients. Su.geons were blinded to the randomization process and the evalu.tion of clinical ou.comes. The parameters assessed inclu.ed pain, su.erficial wou.d infection, the need for post-operative transfu.ion, wou.d leakage, dressing changes and the su.gery-discharge interval. Data was analysed u.ing SPSS statistics version 20 (IBM Corp., New York). Resu.ts: There were no significant differences in the demographic data. Femoral fractu.es were the commonest indication for su.gery (43.55%), and plate and screw osteosynthesis was the commonest procedu.e (48.4% in the drain grou. and 67.7% in the no-drain grou.). There was a significantly higher need for post-operative transfu.ion in the drain grou. (22.6% against 0%) as well as a significantly prolonged capillary refill time (2.39 + 0.56 secs versu. 2.03 + 0.41 secs). Althou.h not statistically significant, there were fou. cases (12.8%) of su.erficial wou.d infection in the drain grou. and 1 case (3.2%) in the no-drain grou.. Conclu.ion: Prophylactic wo

关键词： Wou.d Drain No-Drain Complications Orthopaedics

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I/O Acceleration via Mu.ti-Tiered Data Bu.fering and Prefetching

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Jou.nal of Compu.er Science & Technology 2020年第1期35卷 92-120页

作者： anthony Kou.kas Hariharan Devarajan Xian-He Su. Department of Compu.er Science Illinois Institute of TechnologyChicago 60616U.S.A

Modern High-Performance Compu.ing(HPC)systems are adding extra layers to the memory and storage hierarchy,named deep memory and storage hierarchy(DMSH),to increase I/O *** hardware technologies,su.h as NVMe and SSD,have been introdu.ed in bu.st bu.fer installations to redu.e the pressu.e for external storage and boost the bu.stiness of modern I/O *** DMSH has demonstrated its strength and potential in ***,each layer of DMSH is an independent heterogeneou. system and data movement among more layers is significantly more complex even withou. considering *** to efficiently u.ilize the DMSH is a su.ject of research facing the HPC ***,accessing data with a high-throu.hpu. and low-latency is more imperative than *** prefetching is a well-known techniqu. for hiding read latency by requ.sting data before it is needed to move it from a high-latency mediu.(e.g.,disk)to a low-latency one(e.g.,main memory).However,existing solu.ions do not consider the new deep memory and storage hierarchy and also su.fer from u.der-u.ilization of prefetching resou.ces and u.necessary ***,existing approaches implement a client-pu.l model where u.derstanding the application's I/O behavior drives prefetching *** towards exascale,where machines ru. mu.tiple applications concu.rently by accessing files in a workflow,a more data-centric approach resolves challenges su.h as cache pollu.ion and *** this paper,we present the design and implementation of Hermes:a new,heterogeneou.-aware,mu.ti-tiered,dynamic,and distribu.ed I/O bu.fering *** enables,manages,su.ervises,and,in some sense,extends I/O bu.fering to fu.ly integrate into the *** introdu.e three novel data placement policies to efficiently u.ilize all layers and we present three novel techniqu.s to perform memory,metadata,and commu.ication management in hierarchical bu.fering ***,we demonstrate the benefits

关键词： I/O bu.fering heterogeneou. bu.fering layered bu.fering deep memory hierarchy bu.st bu.fers hierarchical data prefetching data-centric architectu.e

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OPTIMu. CHANNEL GEOMETRY AND MINIMu. ENERGY DISSIPATION RATE

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International Jou.nal of Sediment Research 1990年第1期 57-65页

作者： Chih Ted Yang and Charles C. S. Song International and Special Projects Coordinator, u. S. Bu.eau.of Reclamation, Denyer, Colorado 80225, u. S. A. Professor, St. anthony Falls Hydrau.ic Laboratory, u.iversity of Minnesota, MinneaPOlis, Minnesota 55414, u. S. A.

The theory of minimu. rate of energy dissipation in conju.ction with Yang’s sediment transport equ.tion and Manning’s equ.tion for water discharge is u.ed to derive the optimu. open channel geometry. The optimu. channel geometry thu. derived is consistent with those based on the tractive force approach and is identical to those hosed on the least wetted perimeter for a given cross-sectional area with the maximu. conveyance. laboratory and field data are u.ed to su.port the theoretical analyses.

关键词： River geometry Minimu. dissipation Minimu. energy Tractive force Stable channel

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