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Differential Measurements of oxidatively Modified Proteins in Colorectal Adenopolyps

International Journal of Clinical Medicine 2015年第4期6卷 289-299页

作者： Sharifeh Mehrabi Lashanale Wallace Shakeria cohen Xuebiao Yao Felix o. Aikhionbare Department of Medicine Morehouse School of Medicine Atlanta USA Department of Physiology Morehouse School of Medicine Atlanta USA

Introduction: Adenopolyps patients have a three-fold higher risk of colon cancer over the general population, which increases to six-fold if the polyps are multiple and with lower survival among African American population. Currently, 6% of CRC can be ascribed to mutations in particular genes. Moreover, the optimal management of patients with colorectal adenopolyps depends on the accuracy of appropriate staging strategies because patients with similar colorectal adenocarcinoma architecture display heterogeneity in the course and outcome of the disease. oxidative stress, due to an imbalance between reactive oxygen species (RoS) and antioxidant capacities as well as a disruption of redox signaling, causes a wide range of damage to DNA, proteins, and lipids which promote tumor formation. objective/Method: This study applied spectrophotometric, dinitrophenylhydrazone (DNPH) assay, two-dimensional gel electrophoresis, and western blot analyses to assess the levels of oxidatively modified proteins in 41 pairs of primary colorectal tissues including normal/surrounding, adenopolyps (tubular, tubulovillous, villous, polypvillous) and carcinoma. Analysis of variance (ANoVA) and Student’s t-tests were utilized for the resulting data set. Results: our data showed that the levels of reactive protein carbonyl groups significantly increased as colorectal adenopolyps progresses to malignancy. No significant differences were found in the levels of carbonyl proteins between gender samples analyzed. For African American patients, there were, relative to Caucasians, 10% higher levels of reactive carbonyls in proteins of tubulovillous tissue samples

关键词： Colorectal Adenopolyps oxidative Stress Carbonyl Proteins

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Sequence-based Functional Metagenomics Reveals Novel Natural Diversity of Functional CopA in Environmental Microbiomes

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1182-1194页

作者： Wenjun Li Likun Wang Xiaofang Li Xin Zheng Michael F.cohen Yong-Xin Liu Hebei Key Laboratory of Soil Ecology Centre for Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China University of Chinese Academy of Sciences Beijing 100049China Department of Biology Sonoma State UniversityRohnert ParkCA 94928USA State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Exploring the natural diversity of functional genes/proteins from environmental DNA in high throughput remains *** this study,we developed a sequence-based functional metagenomics procedure for mining the diversity of copper(Cu)resistance gene copA in global microbiomes,by combining the metagenomic assembly technology,local BLAST,evolutionary trace analysis(ETA),chemical synthesis,and conventional functional *** total,87 metagenomes were collected from a public database and subjected to copA detection,resulting in 93,899 *** curation of 1214 hits of high confidence led to the retrieval of 517 unique CopA candidates,which were further subjected to ***,175 novel copA sequences of high quality were *** analysis showed that almost all these putative CopA proteins were distantly related to known CopA proteins,with 55 sequences from totally unknown *** novel and three known copA genes were chemically synthesized for further functional genomic tests using the Cu-sensitive Escherichia coli(DcopA).The growth test and Cu uptake determination showed that five novel clones had positive effects on host Cu resistance and *** recombinant harboring copA-like 15(copAL15)successfully restored Cu resistance of the host with a substantially enhanced Cu *** novel copA genes were fused with the gfp gene and expressed in *** for microscopic *** results showed that they were successfully expressed and their proteins were localized to the *** results here greatly expand the diversity of known CopA proteins,and the sequence-based procedure developed overcomes biases in length,screening methods,and abundance of conventional functional metagenomics.

关键词： Functional metagenomics Natural diversity CopA Evolutionary trace analysis Cu resistance

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Astrometric calibration and source characterisation of the latest generation neuromorphic event-based cameras for space imaging

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Astrodynamics 2023年第4期7卷 415-443页

作者： Nicholas owen Ralph Alexandre Marcireau Saeed Afshar Nicholas Tothill Andre van Schaik Gregory cohen International Centre for Neuromorphic Systems MARCS Institute for BrainBehaviour and DevelopmentWestern Sydney UniversityKingswood 2747NSWAustralia

As an emerging approach to space situational awareness and space imaging,the practical use of an event-based camera(EBC)in space imaging for precise source analysis is still in its *** nature of event-based space imaging and data collection needs to be further explored to develop more effective event-based space imaging systems and advance the capabilities of event-based tracking systems with improved target measurement ***,for event measurements to be meaningful,a framework must be investigated for EBC calibration to project events from pixel array coordinates in the image plane to coordinates in a target resident space object’s reference *** this paper,the traditional techniques of conventional astronomy are reconsidered to properly utilise the EBC for space imaging and space situational *** paper presents the techniques and systems used for calibrating an EBC for reliable and accurate measurement *** techniques are vital in building event-based space imaging systems capable of real-world space situational awareness *** calibrating sources detected using the EBC,the spatiotemporal characteristics of detected sources or“event sources”can be related to the photometric characteristics of the underlying astrophysical ***,these characteristics are analysed to establish a foundation for principled processing and observing techniques which appropriately exploit the capabilities of the EBC.

关键词： neuromorphic event-based camera(EBC) space imaging space situational awareness(SSA) astronomy

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Bacterial microcosms obey Taylor’s law:effects of abiotic and biotic stress and genetics on mean and variance of population density

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Ecological Processes 2012年第1期1卷 77-82页

作者： oliver Kaltz Patricia Escobar-Páramo Michael E Hochberg Joel E cohen Institut des Sciences de l’Evolution UMR CNRS-UM2-IRD5554CC 065UniversitéMontpellier 2Place Eugène BataillonMontpellier Cedex 0534095France Centro Internacional de Física(CIF) Biotechnology GroupUniversidad Nacional de ColombiaCra 30 N°45-03BogotáDCColombia Laboratory of Populations Rockefeller and Columbia Universities1230 York AvenueBox 20New YorkNY10065-6399USA

Introduction:According to the empirical regularity called Taylor’s law,the variance of population density in samples of populations is a power of the mean population *** exponent is often between 1 and *** experiments investigated how genetics,evolution,and environment shape Taylor’s ***:Genetically different strains(wild type and hypermutator)of the bacterium Pseudomonas fluorescens evolved and were assayed under different environmental conditions(with and without antibiotic rifampicin and bacteriophage SBW252,separately and in combination).Results:Experimental treatments altered the exponent b,but not the power law form,of the relation between variance and mean population *** populations treated only with rifampicin had a narrow range of mean population densities and exponent b=*** exposed to rifampicin plus phage had b=*** ancestral,control,and phage-exposed populations,mean abundance varied widely and b was not significantly different from *** factors(mutation rate,selection)and ecological factors(abiotic,biotic)jointly influenced ***:Taylor’s power law relationship accurately and robustly described variance as a function of mean population density,with overall exponent b=*** and other experiments with different factors acting on bacterial population size support the relevance of models that predict‘universal’patterns of fluctuation scaling.

关键词： Taylor’s law Pseudomonas fluorescens rifampicin bacteriophage genetics

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基于TRoPoMI No_(2)、Co及HCHo重构数据的近地面o_(3)浓度估算研究

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遥感学报 2024年第9期28卷 2348-2361页

作者：陈小娟秦凯 cohen Jason 何秦中国矿业大学环境与测绘学院徐州221116

以TRoPoMI大气成分产品为代表的卫星遥感数据在近地面臭氧(o_(3))浓度估算中表现出很好的潜力。由于云及反演算法局限性等原因,TRoPoMI的大气成分产品存在一定的数据缺失,使得基于此的近地面o_(3)浓度估算数据覆盖度较低。为此,本文采... 详细信息

以TRoPoMI大气成分产品为代表的卫星遥感数据在近地面臭氧(o_(3))浓度估算中表现出很好的潜力。由于云及反演算法局限性等原因,TRoPoMI的大气成分产品存在一定的数据缺失,使得基于此的近地面o_(3)浓度估算数据覆盖度较低。为此,本文采用经验正交函数分解插值法(DINEoF)对TRoPoMI No_(2)、Co、HCHo原始数据产品进行缺失像元重构,并基于重构数据产品采用极限梯度提升算法(XGBoost)估算了中国大陆地区2019年—2021年高覆盖度的日最大8 h平均o_(3)浓度(MDA8 o_(3))。对比研究了DINEoF方法对TRoPoMI No_(2)、Co、HCHo原始数据产品的缺失像元进行重构后再建模估算o_(3)的方法(方案1),使用TRoPoMI No_(2)、Co、HCHo原始数据产品并对其缺失像元赋空值并输入其他特征变量来建模估算o_(3)的方法(方案2),有TRoPoMI No_(2)、Co、HCHo原始数据产品的建模结果和无TRoPoMI No_(2)、Co、HCHo原始数据产品,只有其他特征变量的建模结果相结合的方法(方案3),均可提升o_(3)模型估算结果的覆盖度。实验表明:方案1的结果最好,其十折交叉验证R^(2)=0.86,RMSE=15.86μg/m^(3),模型精度和方案2基本一致且高于方案3,在重构区域的模型精度最高(训练集R^(2)=0.82,RMSE=15.57μg/m^(3)),且当重构区域出现o_(3)重污染时(浓度大于160μg/m^(3)),能明显改善模型高值低估现象,结果的空间分布更合理。方案1估算的2019年—2021年近地面MDA8 o_(3)的平均覆盖度从33.6%提升到97.2%,使用TRoPoMI No_(2)、Co、HCHo重构数据产品建模估算o_(3)可提升模型性能和模型结果的覆盖度。

关键词：遥感近地面o_(3) TRoPoMI DINEoF XGBoost 数据重构

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Development of a short duration method to assess the envelope thermal performance of multi-family housings

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Building Simulation 2023年第4期16卷 527-545页

作者： Lorena de Carvalho Araujo Simon Thébault Laurent Mora Thomas Recht Alain Sempey Patrick Schalbart Michae cohen CSTB 84 Av.Jean JauresChamps-sur-Marne77420France Bordeaux University 351 Cours de la LiberationTalence33405France MINES ParisTech Centre for Energy Efficiency of Systems5 rue Léon BlumPalaiseau91120France

Building energy efficiency is a key factor in reducing Co_(2) *** this reason,European Union(EU)member states have developed thermal regulations to ensure building thermal *** results are often based on results achieved with building simulation software during the design ***,the actual thermal performance can deviate significantly from the predicted one,and this difference is known as the energy performance *** indicators of the actual thermal performance are a valuable tool to guarantee building *** indicators,including the heat transfer coefficient(HTC)and the heat loss coefficient(HLC),can be estimated by the application of in situ *** multi-family housing and tertiary sector buildings are an important part of the building stock,mature methods to measure their thermal performance are *** paper presents a short-duration method for assessing the HTC in large building typologies using a sampling *** method was applied in a four-storey building model under different conditions to study the limits of the method and to improve indicator bias and *** quality was strongly influenced by the external weather conditions,the temperature variation during the protocol and the heat exchange with the adjacent *** winter conditions and with stable indoor temperatures,the method had a high accuracy when the protocol was applied for half a *** is recommended that the protocol be used over two days to improve indicator quality under less favorable test conditions.

关键词： building envelope thermal performance HTC estimation multi-family housing

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Evolution of local perforator flaps in lower extremity reconstruction

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Plastic and Aesthetic Research 2019年第12期6卷 1-13页

作者： Rachel cohen-Shohet Mariel McLaughlin David Kerekes Harvey Chim Plastic and Reconstructive Surgery University of FloridaGainesvilleFL 32608USA

Lower extremity reconstruction is challenging for a variety of *** techniques for soft tissue coverage continue to *** free flaps are always an option,free flaps require significant microsurgical expertise,a proficient staff,advanced equipment,and a patient with a somewhat healthy ***,as microsurgery has evolved,so has the identification of new anatomy and new techniques-namely,perforator based pedicled *** flaps have expanded options for lower extremity reconstruction,and continue to advance the field of *** purpose of this article is to review the evolution of perforator based pedicled flaps in the lower extremity,review the anatomy,and offer examples of design and indications.

关键词： Perforator flaps lower extremity reconstruction soft tissue coverage

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Capsule endoscopy in pediatrics: A 10-years journey

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World Journal of Gastroenterology 2014年第44期20卷 16603-16608页

作者： Salvatore oliva Stanley A cohen Giovanni Di Nardo Gianfranco Gualdi Salvatore Cucchiara Emanuele Casciani Department of Pediatrics Pediatric Gastroenterology Unit Sapienza University of Rome Children’s Center for Digestive Health Care Radiology DEA Sapienza University of Rome University Hospital Umberto I

Video capsule endoscopy (CE) for evaluation the esophagus (ECE), small bowel (SBCE) and the colon (CCE) is particularly useful in pediatrics, because this imaging modality does not require ionizing radiation, deep sedation or general anesthesia. The risk of capsule retention appears to be dependent on indication rather than age and parallels the adult experience by indication, making SBCE a relatively safe procedure with a significant diagnostic yield. The newest indication, assessment of mucosal change, greatly enhances and expands its potential benefit. The diagnostic role of CE extends beyond the SB. The use of ECE also may enhance our knowledge of esophageal disease and assist patient care. Colon CCE is a novel minimally invasive and painless endoscopic technique allowing exploration of the colon without need for sedation, rectal intubation and gas insufflation. The limited data on ECE and CCE in pediatrics does not yet allow the same conclusions regarding efficacy; however, both appear to provide safe methods to assess and monitor mucosal change in their respective areas with little discomfort. Moreover, although experience has been limited, the patency capsule may help lessen the potential of capsule retention; and newly researched protocols for bowel cleaning may further enhance CE’s diagnostic yield. However, further research is needed to optimize the use of the various CE procedures in pediatric populations.

关键词： Capsule endoscopy Children Small bowel Pediatric endoscopy

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City of stones

City of stones

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作者： Christopher Valen and Dan cohen.

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Electro-acupuncture to prevent prolonged postoperative ileus:A randomized clinical trial

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World Journal of Gastroenterology 2010年第1期16卷 104-111页

作者： M Kay Garcia Joseph S Chiang Bob Thornton J Lynn Palmer Jennifer McQuade Lorenzo.cohen Department of Anesthesiology and Pain Medicine/Integrative Medicine M.D.Anderson Cancer CenterThe University of Texas Department of Anesthesiology and Pain Medicine M.D.Anderson Cancer CenterThe University of Texas Department of Palliative Care and Rehabilitation Medicine M.D.Anderson Cancer CenterThe University of Texas Department of Behavioral Science M.D.Anderson Cancer CenterThe University of Texas Department of GI Medical oncology M.D. Anderson Cancer CenterThe University of Texas

AIM:To examine whether acupuncture can prevent prolonged postoperative ileus(PPoI)after intraperitoneal surgery for colon cancer. METHoDS:Ninety patients were recruited from the Fudan University Cancer Hospital,Shanghai,China. After surgery,patients were randomized to receive acupuncture(once daily,starting on postoperative day 1, for up to six consecutive days)or usual *** was defined as an inability to pass flatus or have a bowel movement by 96 h after *** main outcomes were time to first flatus,time to first bowel movement, and *** outcomes were quality of life(QoL)measures,including pain, nausea,insomnia,abdominal distension/fullness,and sense of well-being. RESULTS:No significant differences in PPoI on day 4 (P=0.71)or QoL measures were found between the *** were also no group differences when the data were analyzed by examining those whose PPoI had resolved by day 5(P=0.69)or day 6(P= 0.88).No adverse events related to acupuncture were reported. CoNCLUSIoN:Acupuncture did not prevent PPoI andwas not useful for treating PPoI once it had developed in this population.

关键词： Acupuncture Gastrointestinal motility Gastrointestinal disorders Gastrointestinal neoplasms

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