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Steroidal scaffold decorations in Solanum alkaloid biosynthesis

Molecular plant 2024年第8期17卷 1236-1254页

作者： Rosalind Lucier Mohamed o.Kamileen Yoko Nakamura Sofiia Serediuk Ranjit Barbole Jens Wurlitzer Maritta Kunert Sarah Heinicke Sarah e.o'Connor prashant D.Sonawane Department of Natural product Biosynthesis Max Planck Institute for Chemical Ecology07745 JenaGermany Research Group Biosynthesis and NMR Max Planck Institute for Chemical Ecology07745 JenaGermany Biochemical Sciences Division CSIR-National Chemical LaboratoryPune 411008MaharashtraIndia

Steroidal glycoalkaloids(SGAs)are specialized metabolites produced by hundreds of Solanum species,including important vegetable crops such as tomato,potato,and *** it has been known that SGAs play important roles in defense in plants and“anti-nutritional"effects(e.g.,toxicity and bitterness)to humans,many of these molecules have documented anti-cancer,anti-microbial,antiinflammatory,anti-viral,and anti-pyretic *** these,α-solasonine andα-solamargine isolated from black nightshade(Solanum nigrum)are reported to have potent anti-tumor,anti-proliferative,and anti-inflammatory ***,α-solasonine andα-solamargine,along with the core steroidal aglycone solasodine,are the most widespread SGAs produced among the Solanum ***,it is still unknown how plants synthesize these bioactive steroidal *** comparative metabolomictranscriptome-guided approach,biosynthetic logic,combinatorial expression in Nicotiana benthamiana,and functional recombinant enzyme assays,here we report the discovery of 12 enzymes from *** that converts the starting cholesterol precursorto solasodine aglycone,and the downstreamα-solasonine,α-solamargine,and malonyl-solamargine SGA *** further identified six enzymes from cultivated eggplant that catalyze the production ofα-solasonine,α-solamargine,and malonyl-solamargine SGAs from solasodine aglycone via glycosylation and atypical malonylation *** work provides the gene tool box and platform for engineering the production of high-value,steroidal bioactive molecules inheterologoushosts usingsyntheticbiology.

关键词： steroidal glycoalkaloids Solanum biosynthetic pathway α-solamargine malonyl-solamargine specialized metabolites

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effect of radiative loss mechanisms on FIR thermometric parameters of Nd^(3+)-doped lithium tellurite glasses

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Journal of Rare earths 2024年第7期42卷 1250-1257,I0002页

作者： C.Y.Morassuti A.p.L Silva L.A.o.Nunes S.M.Lima L.H.C.Andrade optical and photothermal Spectroscopy Group(GeoF) Center for Studies in Natural Resources(CERNA)State University of Mato Grosso do Sul(UEMS)C.P.351DouradosMSBrazil Renato Archer Information Technology Center Dom Pedro I Highway(SP-65)Km 1436-Chácaras Campos dos AmaraisCampinasSPBrazil Institute of physics of São Carlos University of São PauloSão CarlosSPBrazil

Nd^(3+)-doped tellurite glasses are promising materials for thermometers based on the fluorescence intensity ratio(FIR)***,at high Nd^(3+)concentrations,energy transfer(eT)processes such as optical reabsorption and cross-relaxation can affect the Nd^(3+)emission,which has been little explored in the ***,the present work investigated the use of Nd^(3+)-doped tellurite glass(samples doped with Nd^(3+)at 0.2 mol%,0.5 mol%,2.0 mol%,and 4.0 mol%)in fluorescence thermometers,in the temperature range from 299 to 371 *** results indicate a strong dependence of the FIR parameters on the Nd^(3+)concentration,due to changes in the emission band profiles caused by optical reabsorption of the Nd^(3+)emissions and cross-relaxation processes.A decrease of the relative sensitivity of the ratio^(4)F_(5/2)→^(4)I_(9/2)/^(4)F_(3/2)→^(4)I_(9/2)is observed for samples doped with higher amounts of Nd^(3+).The maximum relative sensitivity at 299 K is 3.00%/K,which is the highest value among the reported Nd^(3+)ions.

关键词： Nd^(3+)-doped tellurite glass Fluorescence intensity ratio optical thermometry Judd-ofelt Rare earths

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europium recovery process by means of polymeric nanoparticles functionalized with acrylic acid,curcumin and fumaramide

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Journal of Rare earths 2024年第10期42卷 1950-1959页

作者： p.A.Martínez-Montoya J.M.del Río A.de J.Morales-Ramirez M.Corea escuela Superior de Ingeniería Química e Industrias extractivas ESIQIE-IPNDepartamento de Ingeniería Metalúrgica UPALM S/N Col.Lindavista escuela Superior de Ingenieria Química e Industrias extractivas ESIQIE-IPNLaboratorio de Investigación en Polímeros y NanomaterialesUPALM S/N Col.Lindavista Centra de Investigación e Innovación Tecnológica CIITEC-IPNCda.de CECATIs/n

polymeric nanoparticles of poly(methyl methacrylate) were obtained by emulsion polymerization techniques in a proce ss of two *** particles were functionalized with acrylic acid,curcumin,and fumaramide and three series of polymeric particles were *** incorporation of functional groups was confirmed by Fourier transform-infrared spectrosocopy(FT-IR) and ultraviolet-visible(UV-Vis) *** spherical morphology of particles with an average diameter of 100 nm was observed by scartning electron microscopy(SeM).The polymeric materials were used for recovery of [eu]from synthetic *** nanoparticles show excellent chelation capacity to trap rare-earth ions,because they recover more than 85% of [eu] at pH of *** images of SeM after extraction process show arrays between particles with larger average particle sizes to 1.5 *** addition,the particles have a good stripping capacity,exceeding 50% of it,maintaining their homogeneity in morphology and good stability in dispersion for the recovery and stripping processes.A pseudo-second model order is obtained for the extraction and stripping processes while the best results of stripping process are obtained at pH of6.

关键词： Chelating effect emulsion polymerization extraction process Rare earths Stripping process [eu]recovery

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paper Analytical Device for the Colourimetric Detection of Alkaline phosphatase in Serum and Saliva

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Journal of Analysis and Testing 2024年第03期8卷 385-394页

作者： D.Alageswari A.LakshmiDevi p.e.Resmi p.V.Suneesh Aarathipradeep T.G.SatheeshBabu Department of Chemistry Amrita School of Physical Sciences Coimbatore Amrita Vishwa Vidyapeetham Amrita Biosensor Research Lab Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham Amrita Biomedical engineering Centre Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham

A paper analytical device (pAD) was developed for the colourimetric estimation of alkaline phosphatase (ALp). ALp catalyzes the hydrolysis of disodium phenyl phosphate (DSpp) to phenol, which then undergoes oxidative coupling with4-aminoantipyrine (4-AAp) producing a reddish brown-coloured quinone imine. The colourimetric reaction tested in solution has been translated onto a pAD for the point of care (poC) testing of ALp. A uniform colour was obtained in 25 min with the introduction of ALp onto the reagent drop cast paper device. The images obtained by scanning the pAD were processed using ImageJ software. The colour intensity obtained by image processing was proportional to the concentration of ALp present in the sample. ALp in serum and saliva samples were tested using the pAD in the range of 0 to 528 U/L and0 to 187 U/L, respectively. The interference studies revealed that the pAD was selective to ALp in the presence of other biomolecules. The pAD exhibited a limit of detection (LoD) of 3.3 U/L and a sensitivity of 48.36 a.u/Log(U/L) for serum ALp and LoD of 1.69 U/L and a sensitivity of 28.5 a.u/Log (U/L) for salivary ALp. The pAD showed good agreement with the clinical methods for real samples.

关键词： Alkaline phosphatase Colourimetry ImageJ paper analytical device point of care testing

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Detailed seismic zoning of the east Kazakhstan region in the Republic of Kazakhstan

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Geodesy and Geodynamics 2024年第2期15卷 156-165页

作者： Natalya V.Silacheva N.p.Stepanenko o.K.Kurilova A.D.Kudabayeva A.T.Danabayeva Institute of Seismology Ministry of Emergency SituationsAlmaty 050060Kazakhstan

Kazakhstan is currently drafting new construction regulations that comply with the major provisions of the *** regulations are created on the basis of seismic zoning maps of various degrees of detail,developed by our Institute of Seismology using a new methodological approach for *** article is about creating the first normative map of the Detailed Seismic Zoning on a probabilistic foundation for the Republic of Kazakhstan’s east Kazakhstan *** carried out the probabilistic assessment of seismic hazard using a methodology consistent with the main provisions of eurocode 8and updated compared with that used in developing maps of Kazakhstan’s General Seismic Zoning and seismic microzoning of *** most thorough and current data accessible for the area under consideration were combined with contemporary analytical *** have been done to not only the databases being used but also the way seismic sources were shown,including active faults *** a scale of 1:1000000,precise seismic zoning maps of the east Kazakhstan region were created for two probabilities of exceedance:10%and 2%in 50 years in terms of peak ground accelerations and macroseismic *** obtained seismic hazard distribution is generally consistent with the General Seismic Zoning of Kazakhstan’s previous ***,because active faults were included and a thoroughly revised catalog was used,there are more pronounced zones of increased danger along the fault in the western part of the *** the west of the territory,acceleration values also increased due to a more accurate consideration of seismotectonic *** maps are the basis for developing new state building regulations of the Republic of Kazakhstan.

关键词： probabilistic seismic hazard assessment Detailed seismic zoning peak ground acceleration Microseismical intensity

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on the analytical solution of transient friction in channel flows

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Applied Mathematics and Mechanics(english edition) 2024年第8期45卷 1447-1466页

作者： F.J.GARCíA-GARCíA p.FARINAS-ALVARINo Department of Naval&Industrial engineering Polytechnic School of EngineeringUniversity of A CorunaCampus Industrial de FerrolFerrol 15403A CorunaSpain Integraciones Técnicas de Seguridad S.A.Pol.Ind.Espiritu SantoC/Nobel15Cambre 15650A CorunaSpain

The transient friction in channel mean flows is the sum of two contributions,i.e.,the underlying laminar flow(ULF)and the purely turbulent component(pTC),and the contributions are analyzed separately by theoretical *** is found that,the transient friction may be higher or remarkably lower than that in equal-Reynolds number steady-state *** universal time constant for plane-parallel laminar flows is reported,and the role of the time constant in a turbulent mean flow is *** is shown that the time constant is related to the turbulence's frozen ***,a study of the logarithmic layer during the transient flow is accomplished,which shows that the logarithmic layer is destroyed.

关键词： transient friction transient flow unsteady channel flow

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Highly Defective Dark Tio_(2) Modified with pt: effects of precursor Nature and preparation Method on photocatalytic properties

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Transactions of Tianjin University 2024年第2期30卷 198-209页

作者： e.D.Fakhrutdinova o.A.Reutova T.A.Bugrova I.Yu.ovsyuk L.S.Kibis o.A.Stonkus D.B.Vasilchenko o.V.Vodyankina V.A.Svetlychnyi National Research Tomsk State University TomskRussia 634050 Novosibirsk State University NovosibirskRussia 630090 Boreskov Institute of Catalysis NovosibirskRussia 630090 Nikolaev Institute of Inorganic Chemistry NovosibirskRussia 630090

The study focused on the modification with platinum of dark defective titania obtained via pulsed laser ablation. Both the method of pt introduction and the nature of the pt precursor were varied. All samples exhibited similar phase compositions, specific surface areas, and pt contents. High-resolution transmission electron microscopy coupled with pulsed Co adsorption revealed increased dispersity when photoreduction and the hydroxonitrate complex (Me _(4) N) _(2) [pt _(2) (oH) _(2) (No _(3) ) _(8) ] were used. The sample featured a high content of single-atom species and subnano-sized pt clusters. The X-ray photoelectron spectroscopy results showed that the photoreduction method facilitated the appearance of a larger number of pt ^(2+) states, which appeared owing to the strong metal-support interaction (SMSI) eff ect of the transfer of electron density from the electron-saturated defects on the Tio _(2) surface to pt ^(4+) . In the hydrogen evolution reaction, samples with a significant fraction of the pt ^(2+) ionic component, capable of generating short-lived pt^(0) single-atom sites under irradiation due to the SMSI eff ect, exhibited the highest photocatalytic activity. The 0.5pt(C)/Tio_(2) -ph sample exhibited the highest hydrogen yield with a quantum efficiency of 0.53, retaining its activity even after 8 h of operation.

关键词： Dark(black)Tio_(2) pulsed laser ablation platinum reduction method precursor type photocatalysis Hydrogen evolution reaction

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practice of Barrier Measures against CoVID-19 Among Diabetic people Followed at Departmental University Hospital Center ouémé-plateau, Benin, in 2021

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open Journal of epidemiology 2024年第2期14卷 249-259页

作者： Yèssito Corine Nadège Houehanou Armand Wanvoègbe Akouodé Thomas Hounto Luc Béhanzin Maurice Agonnoudé David Houéto Thierry Adoukonou National School of public Health (eNATSe) University of Parakou Parakou Benin Department of Internal Medicine University Departmental Hospital Ou&#233me-Plateau Porto-Novo Benin

Introduction: CoVID-19 is a pandemic disease caused by the coronavirus SARS-CoV2 appeared in China in 2019. The aim of the study was to evaluate the practice of barrier measures against CoVID-19 and to identify associated factors among diabetics followed at Departmental University Hospital Center ouémé-plateau, Benin, in 2021. Methods: This was a cross-sectional study conducted from June to August 2021, including diabetics aged 18 and over, hospitalized or received for medical visits in the department of medicine at the target hospital. Data were collected during an interview with each participant thanks to a questionnaire. “Good practice” was defined as regular practice of at least two of three barrier measures: hand washing, physical distancing and mask-wearing. Results: A total of 110 diabetics were included with a female predominance (55.5%) and a mean age of 57 ± 11.3 years. Among them, 89.1% washed their hands, 41.8% respected physical distancing and 12.7% wore a mask. The proportion of good practice was 47.3%. only 5 practiced the three barrier measures. only age group was associated with good practice. Conclusion: The study showed a low proportion of good practice of barrier measures against CoVID-19 among diabetic people surveyed. It is important to continue the action for CoVID-19 prevention among diabetic people in the targeted hospital and in other hospitals in Benin.

关键词： CoVID-19 Barrier Measures practice Diabetic people Benin

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Stability analysis of a time-delayed Van der pol–Helmholtz–Duffing oscillator in fractal space with a non-perturbative approach

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Communications in Theoretical physics 2024年第4期76卷 22-31页

作者： Yusry o el-Dib Department of Mathematics Faculty of EducationAin Shams UniversityRoxyCairoEgypt

The time-delayed fractal Van der pol–Helmholtz–Duffing(VpHD)oscillator is the subject of this paper,which explores its mechanisms and highlights its stability *** time-delayed technologies are currently garnering significant attention,the focus of this research remains crucially relevant.A non-perturbative approach is employed to refine and set the stage for the system under *** innovative methodologies introduced yield an equivalent linear differential equation,mirroring the inherent nonlinearities of the ***,the incorporation of quadratic nonlinearity into the frequency formula represents a cutting-edge *** analytical solution's validity is corroborated using a numerical *** conditions are ascertained through the residual Galerkin ***,it is observed that the delay parameter,in the context of the fractal system,reverses its stabilizing influence,impacting both the amplitude of delayed velocity and the *** analytical solution's precision is underscored by its close alignment with numerical ***,the study reveals that fractal characteristics emulate damping *** its applicability across diverse nonlinear dynamical systems,this non-perturbative approach emerges as a promising avenue for future research.

关键词： Van der pol-Helmholtz-Duffing oscillator fractal space time-delay non-perturbative approach stability analysis el-Dib's frequency formula

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Numerical analysis of high‑speed railway slab tracks using calibrated and validated 3D time‑domain modelling

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Railway engineering Science 2024年第1期32卷 36-58页

作者： A.F.esen o.Laghrouche p.K.Woodward D.Medina‑pineda Q.Corbisez J.Y.Shih D.p.Connolly Institute for High‑Speed Rail and System Integration University of LeedsLeeds LS29JTUK Institute for Infrastructure and environment Heriot-Watt UniversityEdinburgh EH144ASUK Alstom Transport Saint‑OuenFrance Birmingham Centre for Railway Research and education University of BirminghamBirmingham B152TTUK

Concrete slabs are widely used in modern railways to increase the inherent resilient quality of the tracks,provide safe and smooth rides,and reduce the maintenance *** this paper,the elastic performance of a novel slab trackform for high-speed railways is investigated using three-dimensional finite element modelling in *** is then compared to the performance of a ballasted ***,slab and ballasted track models are developed to replicate the full-scale testing of track *** the models are calibrated with the experimental results,the novel slab model is developed and compared against the calibrated slab track *** slab and ballasted track models are then extended to create linear dynamic models,considering the track geodynamics,and simulating train passages at various speeds,for which the Ledsgard documented case was used to validate the *** travelling at low and high speeds are analysed to investigate the track deflections and the wave propagation in the soil,considering the issues associated with critical *** train loading methods are discussed,and the most practical approach is retained and ***,correlations are made between the geotechnical parameters of modern high-speed rail and conventional *** is found that considering the same ground condition,the slab track deflections are considerably smaller than those of the ballasted track at high speeds,while they show similar behaviour at low speeds.

关键词： High-speed railways Slab track New ballastless track Ballasted track Critical speeds Finite element modelling Calibration of numerical models

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