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Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes:Implications in intestinal pathophysiology

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World Journal of Gastroenterology 2007年第47期13卷 6370-6378页

作者： Pinaki Panigrahi Gheorghe T Braileanu Hegang Chen o colin stine Departments of Pediatrics University of Maryland School of Medicine 22 South Greene Street Baltimore MD 21201 United States Department of Epidemiology & Preventive Medicine University of Maryland School of Medicine 22 South Greene Street Baltimore MD 21201 United States Genomics Core University of Maryland School of Medicine 22 South Greene Street Baltimore MD 21201 United States

AIM: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHoDS: A 19 200 gene/expressed sequence tag gene chip was used to examine expression of genes after infection of Caco-2 cells with strains of normal flora E. coli,Lactobacillus plantarum,and a combination of the two. RESULTS: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli . L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection,27 genes were up-regulated and 59 were down-regulated,with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. CoNCLUSIoN: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription,protein biosynthesis,metabolism,cell adhesion,ubiquitination,and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.

关键词： Lactobacillus Escherichia coli Gene expression Probiotic cDNA microarray

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