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Culture and identification of neonatal rat brain-derived neural stem cells

World Journal of Stem Cells 2023年第6期15卷 607-615页

作者： Qing-Zhong Zhou Xiao-Lan Feng Xu-Feng Jia nurul huda binti mohd nor mohd Hezery Bin Harun Da-Xiong Feng Wan Aliaa Wan Sulaiman Department of Orthopedics The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Neurology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia Department of Radiology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Orthopedics The Peoples’Hospital of Jianyang CityJianyang 641400Sichuan ProvinceChina Department of Human Anatomi Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang Selangor43400Malaysia Department of Orthopedics Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia

BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)*** to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively *** To establish a simplified and efficient method for culture and identification of neonatal rat brain-derived *** First,curved tip operating scissors were used to dissect brain tissues from new born rats(2 to 3 d)and the brain tissues were cut into approximately 1 mm^(3)*** the single cell suspension through a nylon mesh(200-mesh)and culture the sections in *** was conducted with TrypLTM Express combined with mechanical tapping and pipetting ***,identify the 5th generation of passaged NSCs as well as the revived NSCs from *** incorporation method was used to detect self-renew and proliferation capabilities of *** NSCs specific antibodies(anti-nestin,NF200,NSE and GFAP antibodies)were used to identify NSCs specific surface markers and muti-differentiation capabilities by immunofluorescence *** Brain derived cells from newborn rats(2 to 3 d)proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable *** BrdU was incorporated into the 5th generation of passaged cells,positive BrdU cells and nestin cells were observed by immunofluorescence *** induction of dissociation using 5%fetal bovine serum,positive NF200,NSE and GFAP cells were observed by immunofluorescence *** This is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification.

关键词： Neonatal rats Brain-derived neural stem cells Culture Identification

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Redefining biofuels:Investigating oil palm biomass as a promising cellulose feedstock for nitrocellulose-based propellant production

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Defence Technology（防务技术） 2024年第7期37卷 111-132页

作者： Khoirul Solehah binti Abdul Rahim Alinda binti Samsuri Siti Hasnawati binti Jamal Siti Aminah binti mohd nor Siti nor Ain binti Rusly Hafizah binti Ariff Nur Shazwani binti Abdul Latif Centre for Tropicalization(CENTROP) Defence Research InstituteUniversiti Pertahanan Nasional MalaysiaKem Sungai Besi57000 Kuala LumpurMalaysia Department of Chemistry and Biology Centre for Defence Foundation StudiesUniversiti Pertahanan Nasional MalaysiaKem Sungai Besi57000 Kuala LumpurMalaysia

This review paper explores the potential of oil palm biomass as a valuable cellulose source for the production of nitrocellulose-based propellants,contributing to the green revolution and sustainable energy *** highlights the availability of the corresponding biomass in Malaysia and in line with global studies,the chemical compositions,as well as a brief description of current technologies for converting biomass of oil palm into value added products specifically *** to achieve maximum utilization of biomass from oil palm industry for cellulose production and prospective source for nitrocellulose-based propellant are also *** methodology section outlines the pretreatment of lignocellulosic fibres,cellulose extraction,and nitrocellulose production ***,the review underscores the prospective of palm oil biomass as a sustainable cellulose source for propellant manufacturing,while acknowledging the need for further research and advancements in the field.

关键词： Biopolymer Cellulose nitrate Energetic materials Green technology Palm oil

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Identification of thermal front dynamics in the northern Malacca Strait using ROMS 3D-model

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Journal of Oceanology and Limnology 2024年第1期42卷 41-57页

作者： Ku nor Afiza Asnida Ku MANSOR Nur Hidayah ROSELI Poh Heng KOK Fariz Syafiq Mohamad ALI mohd Fadzil mohd AKHIR Institute of Oceanography and Environment Universiti Malaysia TerengganuKuala Nerus 21030Malaysia Faculty of Science and Marine Environment Universiti Malaysia TerengganuKuala Nerus 21030Malaysia

The thermal front in the oceanic system is believed to have a significant effect on biological *** an era of climate change,changes in heat regulation between the atmosphere and oceanic interior can alter the characteristics of this important *** the simulation results of the 3D Regional Ocean Modelling System(ROMS),we identified the location of thermal fronts and determined their dynamic variability in the area between the southern Andaman Sea and northern Malacca *** Single Image Edge Detection(SIED)algorithm was used to detect the thermal front from model-derived *** show that a thermal front occurred every year from 2002 to 2012 with the temperature gradient at the location of the front was 0.3°C/*** to the years affected by El Ni?o and negative Indian Ocean Dipole(IOD),the normal years(e.g.,May 2003)show the presence of the thermal front at every selected depth(10,25,50,and 75 m),whereas El Ni?o and negative IOD during 2010 show the presence of the thermal front only at depth of 75 m due to greater warming,leading to the thermocline deepening and enhanced *** May 2003,the thermal front was separated by cooler SST in the southern Andaman Sea and warmer SST in the northern Malacca *** higher SST in the northern Malacca Strait was believed due to the besieged Malacca Strait,which trapped the heat and make it difficult to release while higher chlorophyll a in Malacca Strait is due to the freshwater conduit from nearby rivers(Klang,Langat,Perak,and Selangor).Furthermore,compared to the southern Andaman Sea,the chlorophyll a in the northern Malacca Strait is easier to reach the surface area due to the shallower thermocline,which allows nutrients in the area to reach the surface faster.

关键词： regional ocean modelling system thermal front Andaman Sea Malacca Strait single image edge detection algorithm

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Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrumβ-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia

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Asian Pacific Journal of Tropical Medicine 2024年第1期17卷 30-38页

作者： nurul Syazrah Anuar Hazmin Hazman Sharven Raj Jeyakumar mohd Nasir mohd Desa Hasni Idayu Saidi Siti norbaya Masri Nur Afiza Aziz Nurshahira Sulaiman Department of Biomedical Science Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400UPM SerdangSelangorMalaysia Department of Medical Microbiology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400UPM SerdangSelangorMalaysia Microbiology Unit Department of PathologyHospital Sultanah AminahJalan Persiaran Sultan Abu Bakar80100 Johor BahruJohorMalaysia

Objective:To determine the distribution,phenotypic and genetic background of extended spectrumβ-lactamases(ESBL)-producing Klebsiella(K.)pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in ***:A total of 192 *** isolates were collected and subjected to antibiotic susceptibility,hypermucoviscosity test and multiplex PCR to detect the presence of K1-and K2-serotype associated *** sequence typing(MLST)was performed on ESBL-producing *** isolates presented with K1 and K2 serotypes,followed by phylogenetic ***:A total of 87 out of 192(45.3%)of the *** isolates collected were ESBL ***,only 8.3%(16/192)and 10.9%(21/192)of the total isolates were detected to carry K1-and K2-serotype associated genes,*** analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype(P=0.196).However,they were significantly associated with hypervirulent,as demonstrated by the positive string test(P<0.001).MLST analysis revealed that ST23 as the predominant sequence type(ST)in the K1 serotype,while the ST in the K2 serotype is more ***:Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low,their coexistence warrants the need for continuous *** showed that these isolates were genetically heterogeneous.

关键词： Extended spectrumβ-lactamases Klebsiella pneumoniae Capsular serotypes Genotypic

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Evolution of the“ancient Kedah”:A study on urban forms at Sungai Batu Archaeological Complex(SBAC),Bujang Valley,Kedah,Malaysia

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Frontiers of Architectural Research 2024年第1期13卷 127-143页

作者： mohd Hasfarisham Abd Halim Naizatul Akma mohd Mokhtar nor Khairunnisa Talib Shyeh Sahibul Karamah Masnan Ahmad Fadly Jusoh Mokhtar Saidin Kedah Malaysia Universiti Utara Malaysia KedahMalaysia Universiti Sains Malaysia PenangMalaysia

Archaeological research at the Sungai Batu Archaeological Complex(SBAC),which has been carried out until 2023,aims to complete the chronological data and urbanization process of the ancient *** study will especially prove that the ongoing urbanization process is aimed at developing the iron smelting industry and trade in ancient Kedah,especially in *** is because archaeological research at SBAC has proven that with the existence of the ancient Kedah iron smelting workshop at SBAC,river jetties,port management,rituals,and Buddhist monuments began to be erected,all of which can be determined by chronometric *** results of the chronometric dating have proven that the ancient Kedah Kingdom existed since the 6th century BC and was oriented towards the trade of iron resources,which allowed the use of the area to grow *** process of urbanization and land use clearly conforms to Lynch’s principle,which emphasizes the principles of 1)paths,2)edges,3)districts,4)nodes,and 5)landmarks in the development process of land use and urbanization in the complex.

关键词： Chronometric dating Urbanization Land use Monument Iron smelting site

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Effects of dietary astaxanthin enrichment on enhancing the colour and growth of red tilapia,Oreochromis sp.

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Aquaculture and Fisheries 2024年第1期9卷 52-56页

作者： Zuharlida Tuan Harith Suniza mohd Sukri nor Fatin Syuhada Remlee Fatin Nursabriena mohd Sabir Nik Nur Azwanida Zakaria Faculty of Agro Based Industry Universiti Malaysia Kelantan Jeli CampusJeliKelantan17600Malaysia

The aim of this research was to examine the effects of dietary inclusion of astaxanthin from Haematococcus pluvialis in commercial fish feed on the colour and growth of red tilapia,Oreochromis sp..A 6-week feeding trial was conducted at concentration of astaxanthin at 0%(T1),4%(T2),8%(T3)and 12%(T4)in feed formulation to examine the growth performance,colour pigmentation on skin,texture analysis and carotenoid *** showed an increment of certain growth parameter in fish fed with astaxanthin enriched *** in weight gain(WG),protein efficiency ratio(PER)was obtained in fish fed with astaxanthin enriched compared with *** treatments with astaxanthin-enriched provided higher redness(a*)to skin than negative control that directly influence the colour of the *** can be concluded that inclusion of astaxanthin from green algae into commercial fish feed can be used as an alternative natural carotenoid source in Oreochromis *** to ensure good pigmentation,hence improving the skin colour for more appealing and subsequently increase the market value of the fish.

关键词： Oreochromis sp. Astaxanthin Haematococcus pluvialis

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A review of salivary composition changes induced by fasting and its impact on health

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Food Science and Human Wellness 2024年第1期13卷 50-64页

作者： nurul Fadhilah Kamalul Aripin NIdayu Zahid mohd Aizat Abdul Rahim Hashim Yaacob Parvez I.Haris Zubaidah Haji AbdRahim Rauzah Hashim School of Chemical Engineering College of EngineeringUniversiti Teknologi MARAShah Alam 40450Malaysia Centre for Fundamental and Frontier Sciences in Nanostructure Self-Assembly Department of ChemistryFaculty of ScienceUniversiti MalayaKuala Lumpur 50603Malaysia Department of Oral and Craniofacial Sciences Faculty of DentistryUniversiti MalayaKuala Lumpur 50603Malaysia UMSC Dental Specialist Clinic UM Dentistry TowerUniversiti MalayaMedical CentreKuala Lumpur 50603Malaysia Leicester School of Allied Health Sciences De Montfort UniversityLeicester LE19BHUnited Kingdom Chancellery(Research and Innovation) Universiti MalayaKuala Lumpur 50603Malaysia

Human saliva is an indispensable fluid that maintains a healthy oral cavity which otherwise can lead to oral diseases(dental caries and periodontitis).In addition,salivary metabolites and microbiome profile provide early detection of systemic diseases such as cancer and *** diagnostic has gained popularity due to its non-invasive sampling ***(abstinence from food or drink or both)research for weight loss and improve health is common,but studies using fasting saliva are *** metabolites in fasting saliva have been reported with interesting results,which can be enhanced by considering different confounding *** example,fasting saliva contains higher salivary nitrite,which is related to nitric oxide(NO).NO is a vasodilator supporting the healthy function of endothelial cells and its deficiency is connected to many *** timely supply of NO through exogenous and endogenous means is highlighted and the potential advantage of fasting salivary composition changes in relation to COVID-19 infection is *** review aims to provide a general discussion on the salivary composition,properties,and functions of the whole saliva,including the health benefits of fasting.

关键词： Saliva Fasting Nitrate Nitrite Nitric oxide

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A Comprehensive Classification and Analysis of Geomagnetic Storms Over Solar Cycle 24

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Research in Astronomy and Astrophysics 2024年第8期24卷 77-86页

作者： Raja Adibah Raja Halim Shah nurul Shazana Abdul Hamid Mardina Abdullah Adlyka Annuar Idahwati Sarudin Zahira mohd Radzi Akimasa Yoshikawa Department of Applied Physics Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia Space Science Centre (ANGKASA) Institute of Climate ChangeUniversiti Kebangsaan Malaysia Department of Electrical Electronic and Systems EngineeringFaculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia School of Physics Universiti Sains Malaysia Malaysian Space Agency (MYSA) International Research Center for Space and Planetary Environmental Science (i-SPES) Kyushu University

A geomagnetic storm is a global disturbance of Earth?s magnetosphere,occurring as a result of the interaction with magnetic plasma ejected from the *** considerable research,a comprehensive classification of storms for a complete solar cycle has not yet been fully developed,as most previous studies have been limited to specific storm *** study,therefore,attempted to present complete statistics for solar cycle 24,detailing the occurrence of geomagnetic storm events and classifying them by type of intensity (moderate,intense,and severe),type of complete interval (normal or complex),duration of the recovery phase (rapid or long),and the number of steps in the storm?s *** analysis was applied to data from ground-based magnetometers,which measured the Dst index as provided by the World Data Center for Geomagnetism,Kyoto,*** study identified 211 storm events,comprising moderate (177 events),intense (33 events),and severe (1 event) *** 36%of ICMEs and 23%of CIRs are found to be geoeffective,which caused geomagnetic *** to four-step development of geomagnetic storms was exhibited during the main phase for this solar *** showed the geomagnetic storms developed one or more steps in the main phase,which were probably related to the driver that triggered the geomagnetic storms.A case study was additionally conducted to observe the variations of the ionospheric disturbance dynamo (Ddyn) phenomenon that resulted from the geomagnetic storm event of 2015July *** attenuation of the Ddyn in the equatorial region was analyzed using the H component of geomagnetic field data from stations in the Asian sector (Malaysia and India).The variations in the Ddyn signatures were observed at both stations,with the TIR station (India) showing higher intensity than the LKW station (Malaysia).

关键词： (Sun:) solar-terrestrial relations (Sun:) sunspots Sun: activity Sun: coronal mass ejections (CMEs)

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Short Communication: Enhancing the Drying Process of Microbial-Based Products with a Dehumidifier

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Advances in Microbiology 2024年第6期14卷 333-339页

作者： nor Hidayah Bohari Elya Masya mohd Fishal Ili Bazilah Abd Razak Muhammad Ashraf Arif mohd Nasir Cik mohd Rizuan Zainal Abidin Crop Protection and Biosolutions Department FGV R&D Sdn Bhd Bandar Enstek Malaysia

The development of microbial-based products requires certain criteria for them to be successfully commercialized. The product must meet the following desirable criteria: effectiveness, contamination free, stability, cost-effectiveness, and a prolonged shelf life. Controlling the drying process is crucial for ensuring the stability and durability of the product. The traditional approach, which involved mechanical and natural drying, led to decreased productivity and quality. The objective of this research endeavour was to achieve a dry process enhancement while preserving the microbial quality of Trichoderma asperellum (M103). The temperature and relative humidity during the drying period were monitored under two conditions: with and without a dehumidifier. The results demonstrate that the dehumidifier increases drying period efficiency by up to 63%.

关键词： Dehumidifier Drying Relative Humidity Microbial-Based Product

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Peat properties of a tropical forest reserve adjacent to a fire-break canal

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Journal of Forestry Research 2024年第1期35卷 155-167页

作者： Dayang Nur Sakinah Musa mohd Zahirasri mohd Tohir Xinyan Huang Luqman Chuah Abdullah Mohamad Syazaruddin Md Said Muhammad Firdaus Sulaiman Safety Engineering Interest Group(SEIG) Department of Chemical and Environmental EngineeringFaculty of EngineeringUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia International Tropical Forestry Programme Faculty of Tropical ForestryUniversiti Malaysia SabahSabahMalaysia Department of Land Management Faculty of AgricultureUniversiti Putra MalaysiaSelangorMalaysia Research Centre for Fire Safety Engineering Department of Building Environment and Energy EngineeringThe Hong Kong Polytechnic UniversityHong KongPeople’s Republic of China

Tropical peat comprises decomposed dead plant material and acts like a sponge to absorb water,making it fully ***,drought periods dry it readily and increases its vulnerability to *** fires emit greenhouse gases and particles contributing to haze,and prevention by constructing fire-break canals to reduce fire spread into forest reserves is *** paper aims to determine peat physical and chemical properties near a fire-break canal at different fire frequency *** sampling was conducted at two forest reserves in Malaysia which represent low fire frequency and high fire frequency *** results show that peat properties were not affected by the construction of a fire-break canal,however lignin and cellulose content increased significantly from the distance of the canal in both *** study concluded that fire frequency did not significantly influence peat properties except for *** higher fibre content in the high frequency area did not influence moisture content nor the ability to regain ***,fire frequency might contribute differently to changes in physical and chemical properties,hence management efforts to construct fire-break canals and restoration efforts should protect peatlands from further *** findings will benefit future management and planning for forest reserves.

关键词： Peat fire Peat properties Fire-break canals Forest reserves

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