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Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus

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Asian Pacific Journal of Tropical Biomedicine 2022年第11期12卷 483-494页

作者： nurul Hana Zainal Baharin nur fadhilah khairil mokhtar Mohd Nasir Mohd Desa nurul Diana Dzaraly AbdulRahman Muthanna Mazen M.Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa Laboratory of Halal Science Research Halal Products Research InstituteUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Science University of Technology and Applied SciencesRustaq 10 P.C.329Oman Agro-Biotechnology Institute MalaysiaNational Institutes of Biotechnology Malaysia43400 UPM SerdangSelangorMalaysia Department of Microbiology Faculty of Biotechnology and Biomolecular ScienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

Objective:To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10(Kp10)and Lactococcus lactis Gh1(Gh1)against methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococcus(VRE).Methods:The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration,minimum bactericidal concentration,and time-to-kill *** morphological changes were observed using scanning electron microscopy and transmission electron *** elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE,2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins,and the proton motive force study including the efflux of ATP,pH gradient,and the membrane potential study were ***:MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition *** proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential ***:Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell *** division,cell wall biosynthesis,and protein synthesis are involved in the inhibition mechanism.

关键词： Antimicrobial Proteins Secretome proteins Antibiotic-resistance Paenibacillus polymyxa Kp10 Lactococcus lactis Gh1 Vancomycin-resistant Enterococcus Methicillin-resistant Staphylococcus aureus Mechanism

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Optimized synthesis and photovoltaic performance of TiO_2 nanoparticles for dye-sensitized solar cell

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Particuology 2013年第6期11卷 753-759页

作者： Siti nur fadhilah Zainudin Masturah Markom Huda Abdullah Renata Adami Siti Masrinda Tasirin Department of Chemical Engineering and Process Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia Department of Electrical Electronic and System EngineeringFaculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia Department of Chemical and Food Engineering University of Salerno

This paper presents response surface methodology （RSM） as an efficient approach for modeling and optimizing TiO2 nanoparticles preparation via co-precipitation for dye-sensitized solar cell （DSSC） perfor- mance. Titanium （IV） bis-（acetylacetonate） di-isopropoxide （DIPBAT）, isopropanol and water were used as precursor, solvent and co-solvent, respectively. Molar ratio of water, aging temperature and calcina- tion temperature as preparation factors with main and interaction effects on particle characteristics and performances were investigated, Particle characteristics in terms of primary and secondary sizes, crys- tal orientation and morphology were determined by X-ray diffractometry （XRD） and scanning electron microscopy （SEM）. Band gap energy and power conversion efficiency of DSSCs were used for perfor- mance studies. According to analysis of variance （ANOVA） in response surface methodology （RSM）, all three independent parameters were statistically significant and the final model was accurate. The model predicted maximum power conversion efficiency （0.14%） under the optimal condition of molar ratio of DIPBAT-to-isopropanol-to-water of 1 ： 10：500, aging temperature of 36 C and calcination temperature of 400 ℃. A second set of data was adopted to validate the model at optimal conditions and was found to be 0.14 ± 0.015%, which was very close to the predicted value. This study proves the reliability of the model in identi（ving the optimal condition for maximum performance.

关键词： Titanium dioxide Hydrolysis precipitation Dye-sensitized solar cell Optimization Response surface methodology

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Molecular Dynamics Folding Simulation of β-hairpin Protein （1E0Q）

Molecular Dynamics Folding Simulation of β-hairpin Protein ...

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2011 2nd International Conference on Chemical Engineering and Applications（CCEA 2011）

作者： nur Shima fadhilah Mazlan nurul Bahiyah Ahmad Khairudin Faculty of Chemical Engineering Universiti Teknologi Malaysia Malaysia Japan International Institute of Technology Universiti Teknologi Malaysia International Campus Jalan Semarak 54100 Kuala Lumpur Malaysia

The structure and trajectories of the mutant peptide of ubiquitin （PDB ID:1E0Q） has been studied using Molecular Dynamics （MD） *** investigation of protein folding pathway for protein IE0Q had been carried out using MD Simulation for 50 ns at temperature 325 *** this simulation,the protein 1E0Q has folded into its native β-hairpin structure within 5 *** RMSD value as compared to the NMR structure from the first residue to 17 residues is 2.17 ?.It has been observed that Gly 10 had been responsible to promote β-turn which caused the structure to turn into β-*** secondary structure analysis,it is shown that the residue from Thr 6 to Lys 11 has formed a bend in the *** beta strands has also been found comprising residues Glu 2 to Lys 5 and Ile 13 to Glu 16.

关键词： Molecular Dynamics Protein folding β-hairpin

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Blockchain Governance: The Development and Application of a Framework for Waqf Institutions

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现代会计与审计:英文版 2023年第3期19卷 53-72页

作者： khairil Faizal Khairi nur Hidayah Laili Azuan Ahmad Universiti Sains Islam Malaysia NilaiMalaysia

The effectiveness of waqf administration is impeded by poor management of assets,opacity,and unstandardized *** is expected to solve these issues due to its ability to record and trace every transaction,allowing administrators to discharge their accountability to waqf *** there have been numerous proposals for waqf blockchain,there is no explicit framework for the governance of blockchain in waqf *** propose a governance of blockchain framework for waqf institutions based on IT governance dimensions and blockchain governance dimensions from the *** framework comprises three dimensions(formation and context,actors,and means of governance/decision making)that subsume eight sub-dimensions across three layers and three *** then apply the framework on a blockchain-based waqf organization to examine its *** framework is among the first to be designed for waqf institutions.

关键词： blockchain governance governance of blockchain framework waqf institutions

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