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Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

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Photonics Research 2024年第7期12卷 1564-1573页

作者： SANDRO C.OLIVEIRA mARIA S.SOARES bÁRbARA V.GONÇALVES ANDREIA C.m.RODRIGUES AmADEU m.V.m.SOARES RITA G.SObRAL nuno F.SANTOS JAN NEDOmA PEDRO L.ALmEIDA CARLOS mARQUES CICECO-Aveiro Institute of materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3N Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate Laboratory i4Hb-Institute for Health and bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAm-Centre for Environmental and marine Studies and Department of biology University of Aveiro3810-193 AveiroPortugal Department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3N-CENImAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal Department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DmOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/mL to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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Finite element model with imposed slip surfaces for earth mass safety evaluation

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Journal of Central South University 2014年第9期21卷 3627-3638页

作者： nuno ARAUJO Gaspar J.mACHADO Julio b.mARTINS Hugo m.R.D.SILVA C-TAC Territory Environment and Construction Research Centre School of EngineeringUniversity of Minho Guimaraes 4800-058 Portugal Centre of mathematics School of Sciences University of Minho Guimaraes 4800-058 Portugal

The study of earth masses requires numerical methods that provide the quantification of the safety factor without requiring detrimental assumptions. For that, equilibrium analysis can perform fast computations but require assumptions that limit its potentiality. Limit analysis does not require detrimental assumptions but are numerically demanding. This work provides a new approach that combines the advantage of both the equilibrium method and the limit analysis. The defined hybrid model allows probabilistic analysis and optimization approaches without the assumption of interslice forces. It is compared with a published case and used to perform probabilistic studies in both a homogeneous and a layered foundation. Analyses show that the shape of the density probability functions is highly relevant when computing the probability of failure, and soil elasticity hardly affects the safety of factor of the earth mass.

关键词： slope stability probabilistic methods reliability genetic algorithms safety factor

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Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

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biomaterials Translational 2022年第3期3卷 213-220页

作者： Chavee Laomeephol Helena Ferreira Sorada Kanokpanont Jittima Amie Luckanagul nuno m Neves Siriporn Damrongsakkul Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand biomaterial Engineering for medical and Health Research Unit Chulalongkorn UniversityBangkokThailand b’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineGuimarãesPortugal ICVS/3b’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand biomedical Engineering Research Center Faculty of EngineeringChulalongkorn UniversityBangkokThailand Center of Excellence in Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand

The tissue engineering triad comprises the combination of cells,scaffolds and biological ***,we prepared cell-and drug-loaded hydrogels using in situ silk fibroin(SF)hydrogels induced by dimyristoyl glycerophosphoglycerol(DmPG).DmPG is reported to induce rapid hydrogel formation by SF,facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative *** addition,DmPG can be used for liposome formulations in entrapping drug ***(Dex)was loaded into the DmPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells,then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard *** production by cells cultured in DmPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing *** extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic *** controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic *** results confirmed the possibility of using DmPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.

关键词： DmPG osteogenic differentiation silk fibroin three-dimensional cell culture tissue engineering

Formation of DNA Strand breaks in Peripheral Lymphocytes of Rats After Exposure to Natural Sunlight

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biomedical and Environmental Sciences 2012年第2期25卷 245-249页

作者： Dorival mendes RODRIGUES-JUNIOR Ana Amélia de CARVALHO mELO benedito borges da SILVA Pedro Vitor LOPES-COSTA Department of biology Federal University of Piaui-TeresinaPiauiBrazil Department of Gynecology Federal University of Piaui-TeresinaPiauiBrazil

Objective This paper aims to evaluate the genotoxicity in peripheral blood lymphocytes of rats after exposure to sunlight at different time points of day in a tropical region of brazil（5oS,42oW）.materials and methods Thirty Wistar-Hannover rats,three months old,were randomly divided into three groups of 10 animals each：Group I [control,without exposure to ultraviolet（UV） radiation],Group II（exposed to sunlight during 08：00 *** 10：00 a.m.）,and Group III（exposed to sunlight during 10：00 *** 12：00 a.m.）.After a week of exposure,peripheral blood samples were taken from the tail of these animals to prepare smears on two slides per *** 24 h after exposure to sunlight in Group III,a new collection was obtained to observe the repair *** alkaline comet assay was used in this study to evaluate the genotoxic activity of sunlight（P0.05）.Results There was no statistical difference between Group I and II（P=0.672）.On the other hand,the exposure to sunlight in Group III showed genotoxic action in comparison to the other groups（P0.0001）.Also,there was no significant repair in Group III R（P=0.407）.Conclusion This study has shown a genotoxic potential of sunlight（UVA-b） in lymphocytes of mammals from 10：00 *** 12：00 a.m.,due to a higher intensity of UV in this tropical region.

关键词： Comet assay DNA damage Rats Sunlight Ultraviolet radiation

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multi-mechanical waves against Alzheimer’s disease pathology:a systematic review

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Translational Neurodegeneration 2021年第3期10卷 459-486页

作者： Francisca monteiro Ioannis Sotiropoulos Óscar Carvalho nuno Sousa Filipe S.Silva Center for microelectromechanical Systems(CmEmS) University of MinhoCampus Azurém4800-058 GuimarãesPortugal Life and Health Sciences Research Institute(ICVS) Medical SchoolUniversity of MinhoCampus Gualtar4710-057 BragaPortugal ICVS/3b’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Institute of biosciences&Applications NCSR“Demokritos” AthensGreece

Alzheimer’s disease(AD)is the most common cause of dementia,afecting approximately 40 million people *** inefectiveness of the available pharmacological treatments against AD has fostered researchers to focus on alternative strategies to overcome this *** vibrations delivered in diferent stimulation modes have been associated with marked improvements in cognitive and physical performance in both demented and nondemented *** of the mechanical-based stimulation modalities in eforts are earlier whole-body vibration,transcranial ultrasound stimulation with microbubble injection,and more recently,auditory ***,there is a huge variety of treatment specifcations,and in many cases,conficting results are *** this review,a search on Scopus,Pubmed,and Web of Science databases was performed,resulting in 37 *** studies suggest that mechanical vibrations delivered through diferent stimulation modes are efective in attenuating many parameters of AD pathology including functional connectivity and neuronal circuit integrity defcits in the brains of AD patients,as well as in subjects with cognitive decline and non-demented older *** the evolving preclinical and clinical evidence on these therapeutic modalities,their translation into clinical practice is not consolidated ***,this comprehensive and critical systematic review aims to address the most important gaps in the reviewed protocols and propose optimal regimens for future clinical application.

关键词： Alzheimer’s disease Auditory stimulation mechanical vibrations Ultrasounds Whole-body vibration

Photogrammetric Survey of the Historical Church of Nossa Senhora do Rosário and São benedito in brazil

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Journal of Civil Engineering and Architecture 2020年第10期14卷 572-582页

作者： Luis Antônio Soares e Sousa Claudionor Ribeiro dos Santos marília m.b.Teixeira Vale The Department of Geodetic Sciences Federal University of ParanáParaná81530-000Brazil Institute of Geography Federal University of UberlândiaUberlândia38408-100Brazil Faculty of Architecture and Urbanism and Design Federal University of UberlândiaUberlândia38408-100Brazil

In the history of cities and society,architecture plays an important role in the materialization of buildings,in the ways of life and in the constitution of an *** architectural heritage is an essential task for the conservation,management and collection of knowledge,however it is a time consuming and costly ***,the application of digital aerial and terrestrial photogrammetry has made this process simpler,more agile and of relatively low *** this context,the objective of this study was to analyze the visual and geometric quality of the 3D model,of architectural heritage,generated by photogrammetric *** this end,topographic mapping(reference)and aerial and terrestrial photogrammetric surveys of the Church of Nossa Senhora do Rosário and São benedito,in Estrela do Sul/mG,brazil,which is a historical and cultural heritage of the country,were carried *** as a reference,the 3D topographic model was generated by the points collected with a total *** generate the photogrammetric 3D model,aerial photos were obtained using a UAV(Unmanned Aerial Vehicle)and terrestrial photos,acquired at strategic *** both cases the PowerShot SX260HS camera was *** two georeferenced 3D models were analyzed visually,considering texture and completeness,and compared geometrically through coordinates of homologous ***,the photogrammetric 3D model presented results with a wealth of details far superior to the topographic ones and a geometry with centimetric discrepancies,showing that the method is suitable for generating 3D models relating to architectural heritage.

关键词： Photogrammetry RPAs(Remotely-Piloted Aircrafts) architecture historical patrimony church

2022年欧洲血管外科学会(ESVS)下肢慢性静脉疾病管理临床实践指南(全译)

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中华血管外科杂志 2023年第1期8卷 76-158页

作者： marianne G.De maeseneer Stavros K.Kakkos Thomas Aherne Niels baekgaard Stephen black Lena blomgren Athanasios Giannoukas manjit Gohel Rick de Graaf Claudine Hamel-Desnos Arkadiusz Jawien Aleksandra Jaworucka-Kaczorowska Christopher R.Lattimer Giovanni mosti Thomas Noppeney marie JoseevanRijn Gerard Stansby 无 Philippe Kolh Frederico bastos Gongalves Nabil Chakfé Raphael Coscas Gert J.deborst nuno V.Dias Robert J.Hinchliffe Igor b.Koncar Jes S.Lindholt Santi Trimarchi Rikka Tulamo Christopher P.Twine Frank Vermassen Anders Wanhainen martin bjorck Nicos Labropoulos Fedor Lurie Armando mansilha Isaac K.Nyamekye marta Ramirez Ortega Jorge H.Ulloa Tomasz Urbanek Andre m.van Rij marc E.Vuylsteke 荷兰鹿特丹伊拉斯谟医疗中心皮肤科希腊帕特雷帕特雷大学血管外科爱尔兰科尔克大学医院和爱尔兰国立大学戈尔韦分校血管外科丹麦哥本哈根大学根措夫特医院和国家教学研究医院血管外科英国伦敦盖伊和圣托马斯医院及国王学院瑞典厄勒布罗大学医学院心血管和胸外科希腊塞萨利大学健康科学学院医学院拉里萨大学医院血管外科英国伦敦剑桥大学医院和帝国理工学院德国弗里德里希沙芬诊所诊断和介入放射科法国巴黎圣约瑟夫医院集团法国卡昂圣马丁私立医院血管内科波兰托伦市路德维克·赖德吉耶医学院尼古拉·哥白尼大学血管外科和血管学系波兰戈佐市静脉和美容医学中心、外科和妇科中心伦敦西北大学医疗保健NHS信托基金会英国伦敦帝国学院意大利卢卡maria Domenica barbantini诊所血管科纽伦堡marthamaria医院血管和血管内外科德国雷根斯堡大学医院血管和内皮外科荷兰鹿特丹伊拉斯谟医疗中心血管外科英国泰恩河畔纽卡斯尔弗里曼医院纽卡斯尔大学 ESVS Guidelines Committee 比利时利日利日大学生物医学和临床前科学系葡萄牙里斯本诺瓦德里斯本大学圣玛尔塔医院里斯本中心医院和诺瓦医学院法国斯特拉斯堡大学血管外科和肾移植系巴黎援助公社(AP-HP)CHU AmbroiseParé 法国布洛涅比兰科特巴黎萨克莱大学凡尔赛大学圣昆廷分校血管外科荷兰乌得勒支大学医学中心血管外科瑞典马尔默隆德大学斯科纳大学医院血管中心和马尔默临床科学英国布里斯托尔布里斯托尔大学塞尔维亚贝尔格莱德大学医学院塞尔维亚临床中心血管和血管内外科诊所丹麦欧登塞大学医院心胸血管外科意大利米兰大学临床与社区科学系米兰IRCCS CaGranda Ospedae maggiore Policlinico基金会芬兰赫尔辛基赫尔辛基大学医院和赫尔辛基大学英国布里斯托尔北布里斯托尔NHS信托基金会布里斯托尔大学比利特根特大学医院瑞典乌普萨拉大学外科学系瑞典乌梅奥大学外科和围手术期科学系爱沙尼亚塔尔图塔尔图大学临床医学研究所外科学系美国纽约州石溪市石溪大学医学中心美国俄亥俄州托莱多普惠约布斯特血管研究所密歇根大学葡萄牙波尔图大学医学院英国伍斯特伍斯特郡皇家医院伍斯特郡急症医院NHS信托西班牙马德里La Luz Quiron Salud医院哥伦比亚波哥大安第斯大学圣菲基金会波兰卡托维兹西里西亚医科大学普通外科、血管外科、血管和静脉科新西兰达尼丁奥塔哥大学外科学系比利时Tielt圣安德列齐肯胡斯血管外科

2022年指南与2015年指南相比的新内容是什么?与2015年版《慢性静脉疾病(chronic venous disease,CVD)管理指南》1相比,该指南的总体结构做了很大修改,使其更加实用和方便。在不同章节中增加了关于管理策略的相关内容,并附有流程图。

关键词：临床实践指南血管外科学慢性静脉疾病管理指南流程图总体结构管理策略