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多种转谷氨酰胺酶在人结肠的表达差异:溃疡性结肠炎中受损角化细胞转谷氨酰胺酶的表达

世界核心医学期刊文摘（胃肠病学分册） 2005年第8期1卷 41-42页

作者： D’Arg.nio g.Calvani M Della Valle N g.Peluso 李翔 National Cance r Institute-g Pascale FoundationDepartment of Experimental Oncology Naples Italy.Prof

Backg.ound and aims: Ulcerative colitis (UC) is characterised by refractory in flammatory ulceration and damag. to the *** mechanisms underlying.impaired healing.have yet to be defined. As transg.utaminase expression resulting.in mat rix protein cross linking.is associated with increased wound healing.in a rat mo del of colitis, we hypothesised that different types of transg.utaminase mig.t a lso play a role in UC. Patients end methods: Endoscopic and histolog.cal indices were studied in 26 patients with UC (10 active and 16 inactive) and in 20 norma l controls underg.ing.colonoscopy. Transg.utaminase activity was evaluated in pl asma (factor XIIIa) by a radioenzymatic metho- d. Factor XIIIa, tissue and keratinocyte transg.utaminase protein content, and mRNA expression in the colon were evaluated by western blot analysis and semiqu antitative reverse transcription-polymerase chain reaction (RT-PCR),respectively. Colon ic location of transg.utaminases and their reaction products, the -(γ-g.utamy l)lysine bonds, was evaluated by immunohistochemistry using.specific monoclonal ***: Transg.utaminase activity was sig.ificantly lower in the pla sma of patients with active UC (4.2 (2.4) mU/ml;pg.utaminase and factor XIIIa were uncha ng.d in active UC compared with inactive disease and controls, while the keratin ocyte form was reduced in active UC. Tissue transg.utaminase and factor XIIIa im munostaining.was strong.y present in damag.d areas colocalising.with isopeptide bonds. In contrast, the keratinocyte form was almost absent in active UC and loc alised in the upper part of the crypts in normal subjects. RT-PCR showed upreg. lation of tissue transg.utaminase mRNA in active UC (320%compared with controls ) while keratinocyte transg.utaminase g.ne expression was downreg.lated in activ e UC. Conclusion

关键词：溃疡性结肠炎结肠镜检角化细胞转谷氨酰胺酶大鼠结肠炎模型炎性溃疡活动型组织形态学肠隐窝组织型

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外观健康的癌症生存者的运动能力

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世界核心医学期刊文摘（儿科学分册） 2006年第5期2卷 9-9页

作者： De Caro E. Fioredda F. Calevo M.g. g. Pong.g.ione 张振 Dept. of Cardiolog. G. Gaslini Children's Hospital Largo Gerolamo Gaslini 5 16148 Genoa Italy.Dr

Aims: To evaluate cardiopulmonary exercise tolerance in a larg. cohort of appa rently healthy paediatric cancer survivors in order to determine their participa tion in sporting.activities. Methods: A total of 84 young.(g.m2) and/or chest irradiation (median dose 2000 cg.), with norma l left ventricular systolic function at rest (fractional shortening.> 29%), and 79 healthy controls were studied. Exercise testing.was performed on a treadmill erg.meter. g.s exchang. analysis and derived variables were measured on a breat h-by-breath basis. Pulmonary functional evaluation was performed before exerci se. Echocardiog.aphic evaluation at rest was performed within one month before t he exercise test. Results: There were no differences in exercise responses betwe en patients and controls. In boys g.tly but sig. ificantly lower than in controls. This finding.was thoug.t to be a result of dec reased physical fitness as all the other exercise parameters were similar to those in the controls. Conclusions: Results show that apparently healthy survivors of paediatric cancer can take part in dynamic sporting.activities if they exhibi t a normal response to cardiopulmonary exercise testing. while those that exhibi t a reduced VO2 max should be re-evaluated after an aerobic training.prog.amme, and should underg. tailored dynamic physical activity if the VO2 max does not n ormalise.

关键词：癌症生存者运动能力健康外观左室收缩功能正常左室短轴缩短率平均剂量蒽环类药物体育活动运动耐量

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评估长期使用羟化氯奎患者的多焦视网膜电图的改变

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世界核心医学期刊文摘（眼科学分册） 2005年第1期1卷 23-24页

作者：邢咏新 Maturi R.K Yu M Weleber R.g Midwest Eye Institute 201P ennsylvani-a PkwyIndianapolis IN 46280Unit ed States.Dr.

Objectives:To observe the long.term effects of hydroxy-chloroquine sulfate on retinal electrical activity by multi-focal electroretinog.aphy(mfERg.and to evaluate the re-g.onal variation of retinal dysfunction in subjects with hy-droxychloroquine ***:Multifocal ERg.with 103-hexag.n stimulation was performed on 19patients(36eyes)treated with hydroxychloroquine fo r systemic lupus erythematosus,rheumatoid arthritis,or localized atypical *** acuity testing.Amsler g.id testing.and Ishihara color vision t esting.were also *** 2of the patients,hydroxy chloroquine was dis-continued due to concerns about *** of these patients had additional mfERg.perfo rmed after discontinu-ation of ***:Twelv e patients(19eyes)had a normal response density in one o r both eyes,including.6patients(12eyes)with a low lifetime dose(≤438g.)of hydroxychloroquine who had norma l response densities in both *** patie nts(17eyes)had ab-normal response densities in one or b oth eyes,and 2of these patients(4eyes)had sig.ificant attenuation of re-sponse densities in almost the whole tested field;4patients had a normal mfERg.result for one eye b ut had a slig.t decrease of response densities for t he other *** were4patterns of abnormal mfERg.amplitu de chang. observed:(1)paracentral loss,(2)foveal loss,(3)peripheral loss,and(4)g.neralized *** times we re abnormal for pericentral responses in *** results of color vision and Amsler g.id testing.were n ormal,except for one patient with a g.neralized loss patt *** 2subjects in whom hydroxychloroquine toxicity was suspected,response densities improved after terminati on of ***:Long.term hydroxychl oroquine use may be associated with mfERg.abnormalitie *** mfERg.appears to detect retinal physiolog.cal chang. earlier than visual acuity testing.color vision testin g.or Amsler g.id testing.*** g.eatest value of the mfERg.i s in d

关键词：视网膜电图羟化视网膜功能对侧眼色觉检查视野缺损视野检查中心凹系统性红斑狼疮毒性反应

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皮肤鳞状细胞癌及癌前病变中环氧化酶-2的表达及血管形成研究:35例免疫组化对照研究

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 54-55页

作者： Nijsten T. Colpaert C.g. Vermeulen P.B. 党倩丽 Department of Patholog. University of Antwerp Antwerp Belgium Dr.

Backg.ound: Cyclooxyg.nase (COX)- 2 expression and tumour-induced ang.og.n esis appear to be increased in squamous cell carcinoma (SCC) of the skin. In oth er cancers, COX- 2 is a pro-ang.og.nic factor. The association between ang.og.enesis and COX- 2 has not been studied in skin cancer. Objectives: To assess th e onset of increased COX- 2 expression and ang.og.nesis in the multistag. carcinog.nesis of SCC as well as the correlation between those two parameters. Patients/methods: We performed a retrospective paired immunohistoch emical analysis of normal skin, actinic keratosis (AK), Bowen’s disease (BD) a nd SCC among.35 individuals. Specimens were considered COX- 2 immunopositive wh en 5% or more of the tumour cells showed clear evidence of immunostaining. To quantify active ang.og.nesis, we used a Ki- 67- CD34 double-labelling.immuno histochemical stain and calculated the fraction of proliferating.endothelial cel ls. The Chalkley method was used to determine the microvessel density. To detect hypoxia, a carboanhydrase IX immunostain was used. Results: Compared with norma l epidermis (0% ), AK (31% ), BD (22% ) and SCC (40% )- were sig.ificantly more likely to be COX- 2 immunopositive (P g.endothelial cells and the Chalkley scores paralleled multistag. carcinog.nes is (P g.s). COX- 2 immunopositivity was fairly co rrelatedwith hypoxia and hig.er proliferating.endothelial cell fractions but not with Chalkley counts. Conclusions: Induction of COX- 2 expression and ang.og.n esis are both early events in the development of SCC. In addition to ultraviolet lig.t,hypoxia and COX- 2 may be involved in skin tumour ang.og.nesis.

关键词：皮肤鳞状细胞癌癌前病变环氧化酶免疫染色博温病光化性角化病有显著性差异统计学结果上皮细胞组织缺氧

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动态定位检测对食管酸反流性疾病的诊断

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世界核心医学期刊文摘（胃肠病学分册） 2005年第8期1卷 10-10页

作者： Schoweng.rdt C.g.郝筱倩 1246 Ashland Avenue Zanesville OH 43701 United States.Dr

Currently the most widely accepted study for the detection of sig.ificant esop hag.al reflux disease is the 24-hr pH study. It is a problematic study, however . The data obtained from that study have to be analyzed by comparison to a norma l tested population,revalued throug. a weig.ted scale, then judg.d ag.inst recor ded symptoms in order to obtain a valid interpretation. Dynamic position acid re flux testing.has recently been described as a better alternative. In this compar ative study between dynamicposition and 24-hr pH testing.in 64 patients, the dy namic position study was found to be more definitive, more informative,and more efficient in the detection of sig.ificant esophag.- al acid reflux disease.

关键词：反流性疾病定位检测酸反流动态定位对照分析备选方案加权数法能

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