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抽吸获取的脂肪组织中活脂肪细胞、未成活脂肪细胞和其他细胞成分数量的测定

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中国美容整形外科杂志 2011年第7期22卷 I0022-I0023页

作者： Hirotaka Suga Daisuke Matsumoto Keita Inoue Tomokuni Shigeura Hitomi Eto noriyuki aoi Harunosuke Kato Hiroaki Abe Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科 Biomaster公司研究所《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景分析脂肪组织中脂肪细胞和其他细胞成分生存能力和数量的方法仍需进一步评估。方法作者采用抽吸法获得1g脂肪组织，利用离心分离法将脂肪组织分层（顶层、中层和底层），利用烟酸己可碱33342和碘化丙啶为细胞染色，采用二甲氧唑黄... 详细信息

背景分析脂肪组织中脂肪细胞和其他细胞成分生存能力和数量的方法仍需进一步评估。方法作者采用抽吸法获得1g脂肪组织，利用离心分离法将脂肪组织分层（顶层、中层和底层），利用烟酸己可碱33342和碘化丙啶为细胞染色，采用二甲氧唑黄比色法（2，3-bis（2-methoxy-4-nitro-5-sulfophenyl）-5-[（phenyl-amino）carbonyl]-2H-tet-razoliumhydroxide，XTT）和甘油一3．磷酸脱氢酶试验（n=10）评估细胞生存能力和数量。分析配制细胞（脂肪细胞，脂肪基质细胞，和白细胞）数量与XTY和甘油-3-磷酸脱氢酶试验分析结果之间的关联（凡=5）。从相同脂肪组织中分离血管基质部分细胞，并利用多色流式细胞术检测其细胞成分（n=5）。结果烟酸己可碱33342和碘化丙啶染色法将活的脂肪细胞从脂肪粒，死亡脂肪细胞和其他细胞中区分开。作者从1g的抽吸脂肪组织中获得6．9×10^5个未破碎脂肪细胞；原脂肪细胞中有30％发生破碎。XTT和甘油-3-磷酸脱氢酶试验均显示了活脂肪细胞的数量与分析结果之间具有显著相关关系，但是只有甘油-3-磷酸脱氢酶试验结果对脂肪细胞有严格特异性。本研究还发现，脂肪基质细胞与脂肪细胞的比率大于之前研究的描述。结论本研究中单独使用一种或联合使用2种生存能力分析法，均能确定脂肪细胞和其他细胞的数量，但是不采用组织分离而直接评估原始细胞仍有困难。

关键词：脂肪细胞脂肪组织细胞成分抽吸法脂肪基质细胞细胞生存能力多色流式细胞术数

人类脂肪来源干细胞经长期深低温保藏后保留的增殖能力和多能性

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中国美容整形外科杂志 2011年第7期22卷 I0013-I0021页

作者： Koichi Gonda Tomokuni Shigeura Takahiro Sato Daisuke Matsumoto Hirotaka Suga Keita Inoue noriyuki aoi Harunosuke Kato Katsujiro Sato Syoko Murase Isao Koshima Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科 Biomaster公司研究所《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景人类脂肪来源干（问质）细胞作为间叶细胞谱系（包括脂肪、骨骼肌和心肌、骨、软骨、血管）组织缺损的再生疗法的工具，是很有应用前景的。同其他领域所发生的一样（包括造血干细胞和脐带血细胞在基于细胞的治疗方法中的应用），在... 详细信息

背景人类脂肪来源干（问质）细胞作为间叶细胞谱系（包括脂肪、骨骼肌和心肌、骨、软骨、血管）组织缺损的再生疗法的工具，是很有应用前景的。同其他领域所发生的一样（包括造血干细胞和脐带血细胞在基于细胞的治疗方法中的应用），在未来可能的临床应用中，脂肪来源干细胞深低温保藏也许是一项必不可少的基本技术。方法作者从18例患者抽吸获得的脂肪中分离出脂肪来源干细胞，并在干细胞深低温保藏6个月前后分别检测它们的增殖能力和多功能性，深低温保藏过程遵循他们已确定的方案。通过检测培养细胞的倍增时间对细胞增殖能力进行量化分析，通过分析脂肪来源干细胞向成软骨、成骨和成脂谱系细胞的分化确定细胞的多功能性。另外，通过流式细胞术确定细胞表面标记物的表达谱，并对新鲜的和经深低温保藏的脂肪来源干细胞表面标记物的表达谱进行对比。结果经深低温保藏的脂肪来源干细胞完全保存分化成脂肪细胞、成骨细胞、成软骨细胞的能力及增殖能力。流式细胞术分析结果显示深低温保藏前后细胞表面标记物表达谱保持不变。结论在现有的方案下，脂肪来源干细胞至少可深低温保藏6个月，且不损失任何增殖能力和分化潜能。这些结果保证了自体库存的脂肪来源干细胞在未来临床应用的可用性。

关键词：脂肪来源干细胞细胞增殖能力深低温保藏人类多能性表面标记物成软骨细胞流式细胞术

抽吸与切取方法获取的脂肪组织的结构特征和细胞组成的对比研究

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中国美容整形外科杂志 2011年第7期22卷 I0022-I0022页

作者： Hitomi Eto Hirotaka Suga Daisuke Matsumoto Keita Inoue noriyuki aoi Harunosuke Kato Jun Araki Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景脂肪组织是一种容易获取的组织，可当做软组织填充物，也是成人多功能细胞的来源，这种多功能细胞被称为脂肪源性干细胞／基质细胞／祖细胞。然而，由于脂肪细胞和脂肪组织的结构很脆弱，脂肪细胞的许多解剖特征仍不清楚。方法纳入... 详细信息

背景脂肪组织是一种容易获取的组织，可当做软组织填充物，也是成人多功能细胞的来源，这种多功能细胞被称为脂肪源性干细胞／基质细胞／祖细胞。然而，由于脂肪细胞和脂肪组织的结构很脆弱，脂肪细胞的许多解剖特征仍不清楚。方法纳入抽吸方法获取的皮下脂肪组织标本15例，完整皮下脂肪组织标本9例，采用以下方法进行评估：①采用组织全层包埋结合三重荧光染色技术，对活脂肪组织进行三维成像；②甘油一3．磷酸脱氢酶试验；③多色流式细胞术（CD34、CD31和CD45）；④培养血管基质部分细胞的贴壁细胞，以产生脂肪源性基质细胞。结果组织全层包埋方法显示脂肪组织旁存在毛细血管网，抽吸获取的脂肪组织中的毛细血管网被部分破坏。抽吸脂肪组织也缺少大的脉管结构，其小脂肪粒（破碎脂肪组织，P=0．00016）和死亡细胞（P=0．0038）的数量均高于切取脂肪组织。抽吸与切取的脂肪组织中，脂肪细胞数量占全部细胞数的比例均小于20％，且脉管系统相关细胞（包括内皮细胞和脂肪源性基质细胞）占到细胞总量的一半以上。甘油一3．磷酸脱氢酶试验显示，抽吸和切取脂肪组织中破裂的脂肪组织所占百分比分别为大于30％和5％（P=0．032）。多色流失细胞术分析结果显示，抽吸脂肪组织中受污染的血源性细胞（CD45＋）数量在总细胞中所占百分比更大（P=0．0038），抽吸脂肪组织内的脂肪源性基质细胞产量大约是切取脂肪组织的一半（P=0．011）。结论作者研究表明两种组织有不同的结构特征和细胞成分，且抽吸脂肪组织内组织损伤更严重，祖细胞产量也不足。

关键词：脂肪组织细胞组成抽吸方法切取方法结构特征脂肪源性干细胞毛细血管网多色流式细胞术

局部缺血时的脂肪组织重构：脂肪细胞的死亡与干／祖细胞的激活

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中国美容整形外科杂志 2011年第7期22卷 I0023-I0023页

作者： Hirotaka Suga Hitomi Eto noriyuki aoi Harunosuke Kato Jun Araki Kentaro Doi Takuya Higashino Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景许多整形外科手术后（如脂肪移植），脂肪组织开始缺血，导致组织缺氧和营养缺失。然而，很少有研究检测脂肪组织中的缺血和／或缺氧变化的报道。方法切断小鼠体内为腹股沟脂肪垫供血的血管，建立外科手术诱导的缺血模型。利用氧气... 详细信息

背景许多整形外科手术后（如脂肪移植），脂肪组织开始缺血，导致组织缺氧和营养缺失。然而，很少有研究检测脂肪组织中的缺血和／或缺氧变化的报道。方法切断小鼠体内为腹股沟脂肪垫供血的血管，建立外科手术诱导的缺血模型。利用氧气监视器测量脂肪组织的氧分压，利用全标本染色、免疫组化、流式细胞术和蛋白质印迹法分析缺血改变。作者还检测了体外低氧环境下的细胞寿命。结果3种程度缺血模型（轻度、中度、重度）的氧分压水平分别为正常脂肪组织（50．5±1．3）mmHg的75％、55％、20％。在第28天观察到脂肪组织萎缩伴随实质纤维变性，这取决于缺血的严重程度。在第1天观察到中度和重度缺血诱导低氧诱导因子1仪和纤维生长因子2表达的提高，中度和重度缺血还可导致脂肪组织中的退行性改变（如细胞凋亡、坏死、巨噬细胞侵噬）。死亡细胞包括脂肪细胞，血管内皮细胞，血源性细胞，但不包括脂肪源性干／祖细胞。退行性改变后可观察到再生性改变，包括血管形成、脂肪形成和细胞的增殖（包括脂肪源性干／祖细胞、血管内皮细胞和血细胞）。笔者还发现，严重缺氧时在体外用实验方法分化的脂肪细胞发生凋亡和／或坏死，而脂肪源性干／祖细胞仍然存活。结论脂肪组织严重缺血／缺氧导致退行性改变，继而发生相应的组织重构。在缺血环境下，脂肪细胞很容易死亡，而脂肪源性干／祖细胞却非常活跃，并有助于组织修复。

关键词：干/祖细胞脂肪细胞局部缺血组织重构死亡血管内皮细胞退行性改变低氧诱导因子1

Gastro-protective action of lafutidine mediated by capsaicin-sensitive afferent neurons without interaction with TRPV1 and involvement of endogenous prostaglandins

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World Journal of Gastroenterology 2006年第19期12卷 3031-3037页

作者： Kazuhiro Fukushima Yoko aoi Shinichi Kato Koji Takeuchi Department of Pharmacology and Experimental Therapeutics Kyoto Pharmaceutical University MisasagiYamashinaKyoto 607-8414Japan

AIM： Lafutidine, a histamine H2 receptor antagonist, exhibits gastro-protective action mediated by capsaicinsensitive afferent neurons （CSN）. We compared the effect between lafutidine and capsaicin, with respect to the interaction with endogenous prostaglandins （PG）, nitric oxide （NO） and the afferent neurons, including transient receptor potential vanilloid subtype 1 （TRPV1）. METHODS： Male SD rats and C57BL/6 mice, both wildtype and prostacyclin IP receptor knockout animals, were used after 18 h of fasting. Gastric lesions were induced by the po administration of HCl/ethanol （60% in 150 mmol/L HCl） in a volume of 1 mL for rats or 0.3 mL for mice. RESULTS： Both lafutidine and capsaicin （1-10 mg/kg, po） afforded dose-dependent protection against HCI/ ethanol in rats and mice. The effects were attenuated by both the ablation of CSN and pretreatment with NG-nitro- L-arginine methyl ester, yet only the effect of capsaicin was mitigated by prior administration of capsazepine, the TRPV1 antagonist, as well as indomethacin. Lafutidine protected the stomach against HCl/ethanol in IP receptor knockout mice, similar to wild-type animals, while capsaicin failed to afford protection in the animals lacking IP receptors. Neither of these agents affected the mucosal PGE2 or 6-keto PGF1α contents in rat stomachs. Capsaicin evoked an increase in [Ca^2＋]i in rat TRPV1-transfected HEK293 cells while lafutidine did not. CONCLUSION： These results suggest that although both lafutidine and capsaicin exhibit gastro-protective action mediated by CSN, the mode of their effects differs regarding the dependency on endogenous PGs/IP receptors and TRPV1. It is assumed that lafutidine interacts with CSN at yet unidentified sites other than TRPV1.

关键词： Lafutidine A histamine H2-receptor antagonist Gastric protection Prostaglandin Capsaicin-sensirive afferent neuron TRPVt

Integrated biological–physical process for biogas purification effluent treatment

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Journal of Environmental Sciences 2019年第9期31卷 110-122页

作者： Roslan Noorain Tomonori Kindaichi Noriatsu Ozaki Yoshiteru aoi Akiyoshi Ohashi Department of Civil and Environmental Engineering Graduate School of Engineering Hiroshima University 1-4-1 Kagamiyama Higashihiroshima Hiroshima 739-8527 Japan Section of Environmental Engineering Technology Malaysia Institute of Chemical & Bioengineering Technology University Kuala Lumpur Lot 1988 Kawasan Perindustrian Bandar Vendor Taboh Naning 78000 Alor Gajah Melaka Malaysia Department of Molecular Biotechnology Graduate School of Advanced Sciences of Matter Hiroshima University 1-3-1 Kagamiyama Higashihiroshima 739-8530 Japan

Biogas purification via water scrubbing produces effluent containing dissolved CH4, H2S,and CO2, which should be removed to reduce greenhouse gas emissions and increase its potential for water regeneration. In this study, a reactor built with air supplies at the top and bottom was utilized for the treatment of biogas purification effluent through biological oxidation and physical stripping processes. Up to 98% of CH4 was removed through biological treatment at a hydraulic retention time of 2 hr and an upper airflow rate of 2.02 L/day. Additionally, a minimum CH4 concentration of 0.04% with no trace of H2S gas was detected in the off gas. Meanwhile, a white precipitate was captured on the carrier showing the formation of sulfur. According to the developed mathematical model, an upper airflow rate of greater than 2.02 L/day showed a small deterioration in CH4 removal performance after reaching the maximum value, whereas a 50 L/day bottom airflow rate was required to strip the CO2 efficiently and raise the effluent p H from 5.64 to 7.3. Microbiological analysis confirmed the presence of type 1 methanotroph communities dominated by Methylobacter and Methylocaldum. However, bacterial communities promoting sulfide oxidation were dominated by Hyphomicrobium.

关键词： Biogas purification effluent Dissolved methane pH elevation Biological oxidation CO2 stripping

Origins of short gamma-ray bursts deduced from offsets in their host galaxies revisited

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Research in Astronomy and Astrophysics 2012年第9期12卷 1255-1268页

作者： Xiao-Hong Cui Shigehiro Nagataki Junichi aoi Ren-Xin Xu National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China xhcui@ bao.ac cn Yukawa Institute for Theoretical Physics Oiwake-cho Kitashirakawa Sakyo-ku Kyoto606-8502 Japan School of Physics and State Key Laboratory of Nuclear Physics and Technology PekingUniversity Beijing 100871 China

The spatial distribution of short Gamma-ray bursts (GRBs) in their host galaxies provides us with an opportunity to investigate their origins. Based on the currently observed distribution of short GRBs relative to their host galaxies, we obtain the fraction of the component that traces the mergers of binary compact objects and the one that traces star formation rate (such as massive stars) in earlyand late-type host galaxies. From the analysis of projected offset distribution and only based on population synthesis and massive star models, we find that the fraction of massive stars is 0.37+-00..4327 with an error at the 1σ level for a sample with 22 short GRBs in the literature. From these results, it is hard to accept that the origin of short GRBs with observed statistics is well described by current models using only the offset distribution. The uncertainties in observational localizations of short GRBs also strongly affect the resulting fraction.

关键词： gamma-rays: bursts — cosmology: galaxy — methods: statistical

Spontaneous regression of gastric gastrinoma after resection of metastases to the lesser omentum:A case report and review of literature

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World Journal of Gastroenterology 2021年第1期27卷 129-142页

作者： Takeshi Okamoto Takaaki Yoshimoto Nobuyuki Ohike aoi Fujikawa Takayoshi Kanie Katsuyuki Fukuda Department of Gastroenterology St.Luke's International HospitalTokyo 104-8560Japan Department of Pathology Shizuoka Cancer CenterShizuoka 411-8777Japan Department of Surgery St.Luke’s International HospitalTokyo 104-8560Japan Department of Cardiology St.Luke’s International HospitalTokyo 104-8560Japan

BACKGROUND Gastric gastrinoma and spontaneous tumor regression are both very rarely *** report the first case of spontaneous regression of gastric *** SUMMARY A 37-year-old man with a 9-year history of chronic abdominal pain was referred for evaluation of an 8 cm mass in the lesser omentum discovered incidentally on abdominal computed *** tumor was diagnosed as grade 2 neuroendocrine neoplasm(NEN)on endoscopic ultrasound-guided fine-needle *** esophagogastroduodenoscopy revealed a 7 mm red polypoid lesion with central depression in the gastric antrum,also confirmed to be a grade 2 *** removal of the abdominal mass confirmed it to be a metastatic gastrinoma *** gastric lesion was subsequently diagnosed as primary gastric *** months later,the gastric lesion had disappeared without *** patient remains symptom-free with normal fasting serum gastrin and no recurrence of gastrinoma during 36 mo of *** Gastric gastrinoma may arise as a polypoid lesion in the gastric *** regression can rarely occur after biopsy.

关键词： Neuroendocrine neoplasm Functional tumor Biopsy Gastrin Stomach Case report

Biological methane production coupled with sulfur oxidation in a microbial electrosynthesis system without organic substrates

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Journal of Environmental Sciences 2022年第6期34卷 68-78页

作者： Ha T.T.Dinh Hiromi Kambara Shuji Matsushita Yoshiteru aoi Tomonori Kindaichi Noriatsu Ozaki Akiyoshi Ohashi Department of Civil and Environmental Engineering Graduate School of Advanced Science and EngineeringHiroshima University1-4-1KagamiyamaHigashihiroshimaHiroshima 739-8527Japan Faculty of Environment Ho Chi Minh City University of Natural Resources and Environment236 Le Van Sy1 WardTan Binh DistrictHo Chi Minh CityViet Nam Agricultural Technology Research Center Hiroshima Prefectural Technology Research Institute6869HaraHachihonmatsuHigashihiroshimaHiroshima 739-0151Japan Program of Biotechnology Graduate School of Integrated Sciences for LifeHiroshima University1-3-1KagamiyamaHigashihiroshimaHiroshima 739-8530Japan

Methane is produced in a microbial electrosynthesis system(MES) without organic substrates. However, a relatively high applied voltage is required for the bioelectrical *** this study, we demonstrated that electrotrophic methane production at the biocathode was achieved even at a very low voltage of 0.1 V in an MES, in which abiotic HS-oxidized to SO_(4)^(2-) at the anodic carbon-cloth surface coated with platinum powder. In addition, microbial community analysis revealed the most probable pathway for methane production from electrons. First, electrotrophic H_(2) was produced by syntrophic bacteria, such as Syntrophorhabdus, Syntrophobacter, Syntrophus, Leptolinea, and Aminicenantales, with the direct acceptance of electrons at the biocathode. Subsequently, most of the produced H_(2) was converted to acetate by homoacetogens, such as Clostridium and Spirochaeta 2. In conclusion,the majority of the methane was indirectly produced by a large population of acetoclastic methanogens, namely Methanosaeta, via acetate. Further, hydrogenotrophic methanogens,including Methanobacterium and Methanolinea, produced methane via H_(2).

关键词： Bioelectricity Homoacetogens Methane production Microbial electrosynthesis system Sulfur oxidation