检索结果-南通市图书馆

Spontaneous Subdural Hemorrhage due to Ruptured Arteriovenous Malformation in a Child

Journal of Forensic Science and Medicine 2018年第3期4卷 174-178页

作者： nor fadhilah madon Ahmad Hafizam Hasmi Khairul Anuar Zainun Hapizah Mohd Nawawi Department of Forensic Medicine National Institute of Forensic MedicineHospital Kuala LumpurMinistry of Health MalaysiaKuala Lumpur department of Forensic MedicineHospital Sungai BulohJalan Hospital institute of Pathology Laboratory and Forensic MedicineUniversiti Teknologi MARASungai Buloh CampusJalan HospitalSelangorMalaysia

Spontaneous subdural hemorrhage(SDH)is an uncommon nontraumatic bleed of the brain,involving the dura and arachnoid *** commonly occurs due to tearing of bridging veins;however,SDH from the rupture of arteriovenous malformation(AVM)is an uncommon phenomenon,particularly in *** report a case of atypical SDH in a 1-year-old child with AVM and a history of alleged *** revealed SDH in the right parietal and middle cranial fossa without any external or internal injuries to the head,brain,or other parts of the *** examination and immunohistochemical staining of the brain tissue revealed an abnormal distribution of arteries and veins of various sizes and *** a detailed histopathological examination of the brain and meticulous investigation of vascular abnormalities,we conclude the cause of acute SDH to be due to a ruptured AVM.

关键词： Arteriovenous malformations child abuse spontaneous subdural hemorrhage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Selection Vector for Direct Cloning of Proof Reading Polymerase Chain Reaction Products Based on the Lethal <i>ccdB</i>Gene in <i>Escherichia coli </i>

引用

Advances in Microbiology 2013年第1期3卷 14-20页

作者： Pascal Weibel Miriam Ender Jerzy madon Annelies S. Zinkernagel Reto A. Schuepbach Clinic of Infectious Diseases and Hospital Hygiene University Hospital Zurich University of Zurich Zurich Switzerland Surgical Intensive Care Medicine University Hospital Zurich University of Zurich Zurich Switzerland

Introducing PCR products into plasmids vectors is key for molecular techniques. Ideally cloning vectors are easy to construct, modify and propagate, neither require advanced techniques nor special equipment or reagents and efficiently incorporate PCR products at close to zero empty vector background. We provide an easy to engineer self-made cloning vector, neither requiring sophisticated tools or techniques nor advanced cloning knowledge. Through recombination we obtained the pUC18ccdB vector, carrying the ccdB suicide gene within the pUC18 backbone. When SmaI cleaved (within the ccdB) vector was T4 ligated with small (0.2 kbp) and intermediate (1.3 to 2.2 kbp) blunt end PCR-products and transformed into E. coli, the amount of clones with incorporated PCR product was comparable to commercial PCR-cloning kits and at a close to zero PCR product negative background. In conclusion we present a simple, versatile and cheap approach to an efficient “home made” PCR-cloning vector that allows integration of crude blunt end PCR products at close to zero background.

关键词： PCR-Cloning Vector Blunt End ccdB Toxic Gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：