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Use of buffy coat thick films in detecting malaria parasites in patients with negative conventional thick films

Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 301-303页

作者： Chatnapa Duangdee noppadon tangpukdee Srivicha Krudsood Polrat Wilairatana Hospital for Tropical Diseases Bangkok Department of Clinical Tropical Medicine Faculty of Tropical Medicine Department of Tropical Hygiene Faculty of Tropical Medicine

Objective:To determine the frequency of malaria parasite detection from the buffy coal blood films by using capillary tube in falciparum malaria patients with negative conventional thick ***:Thirty six uncomplicated falciparum malaria patients confirmed by conventional thick and thin films were included in the *** patients were treated with artemisinin combination therapy at Hospital for Tropical Diseases,Bangkok,Thailand for 28 *** for conventional blood films were conducted every 6 hours until negative parasitemia,then daily fingerpricks for parasite checks were conducted until the patients were discharged from hospital. Blood samples were also concurrently collected in 3 heparinized capillary tubes at the same time of fingerpricks for conventional blood films when the prior parasitemia was negative on thin films and parasitemia was lower than 50 parasites/200 white blood cells by thick *** first negative conventional thick films were compared with buffy coat thick films for parasite identification. Results:Out of 36 patients with thick films showing negative for asexual forms of parasites, buffy coat films could detect remaining 10 patients(27.8%) with asexual forms of Plasmodium ***:The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8%of patients whose conventional thick films show negative parasitemia.

关键词： Malaria Detection Buffy coat Thick film Malaria parasite Falciparum malaria Plasmodium falciparum Parasitemia Microscopy

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Indicators of fatal outcome in severe Plasmodium falciparum malaria:a study in a tertiary-care hospital in Thailand

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Asian Pacific Journal of Tropical Medicine 2010年第11期3卷 855-859页

作者： noppadon tangpukdee Khin Myat Wai Sant Muangnoicharoen Shigeyuki Kano Nanthaporn Phophak Janram Tiemprasert Srivicha Krudsood Polrat Wilairatana Critical Care Research Unit Department of Clinical Tropical MedicineFaculty of Tropical MedicineMahidol UniversityBangkokThailand Department of Tropical Medicine and Malaria Research InstituteNational Center for Global Health and Medicine(NCGM)TokyoJapan Hospital for Tropical Diseases Faculty of Tropical MedicineMahidol UniversityBangkokThailand Department of Tropical Hygiene Faculty of Tropical MedicineMahidol UniversityBangkokThailand WHO Collaborating Center for Clinical Management of MalariaFaculty of Tropical MedicineMahidol UniversityBangkokThailand WHO Collaborating Center for Clinical Management of Malaria Faculty of Tropical MedicineMahidol UniversityBangkokThailand

Objective:To illustrate the clinical features and investigate the indicators associated with a fatal outcome in adult patients with severe Plasmodium falciparum malaria admitted to the Hospital for Tropical Diseases,Bangkok,***:We studied 202 adult malaria patients admitted to the Intensive Care Unit.A total of 43 clinical variables were identified by univariate and logistic regression analyses,to eliminate confounding ***:Regarding the statistical methods,only 6 variables-jaundice,cerebral malaria,metabolic acidosis,body mass index,initial respiratory rate,and white blood cell count-were significant indicators of death, with adjusted odds ratios(95%CI) of 15.2(2.1-32.3).4.3(2.3-12.6),3.3(2.3-5.7),2.4(1.9-3.5),2.2 (1.5-2.6),and 1.7(1.2-3.1),***:Our study found that jaundice,cerebral malaria,metabolic acidosis,body mass index,initial respiratory rate and white blood cell count were indicators of fatal outcome in severe Plasmodium falciparum *** studies on the fatal indicators in severe malaria need to be compared with data from different geographical areas,to construct practical measures to address potentially fatal indicators in different settings.

关键词： Plasmodium falciparum Severe malaria Fatal indicator Tertiary care Thailand

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Definition of hyperparasitemia in severe falciparum malaria should be updated

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Asian Pacific Journal of Tropical Biomedicine 2013年第7期3卷 586-586页

作者： Polrat Wilairatana noppadon tangpukdee Srivicha Krudsood Department of Clinical Tropical Medicine Faculty of Tropical Medicine Mahidol UniversityThailand Department of Tropical Hygiene Faculty of Tropical Medicine Mahidol UniversityThailand

Hyperparasitemia is one criterion of severe falciparum malariaby World Health Organization(WHO)for more than two decades[1].Although there is a correlation between density of parasittemiaand severity of malaria,some individuals with high parasite countsmay not be severely ill,whereas others with low parasitemia mayhave ultimately fatal ***(more than 5%

关键词： than more Definition of hyperparasitemia in severe falciparum malaria should be updated

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