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Seedless synthesis of gold nanorods with tunable plasmonic peaks beyond 1300 nm

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Chinese Chemical Letters 2022年第5期33卷 2491-2495页

作者： Liangxi Zhu Zhuoxuan Lu Liming Zhang nongyue he State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Key Laboratory of Tropical Disease and Translational Medicine of the Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical CollegeHaikou 571101China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China

Gold nanorods(AuNRs),as relatively common materials used in biomedical areas,have been synthesized by means of many methods.However,the conventional seed-mediated method is limited by complex operations and low yield.Besides,for further applications of AuNRs,well monodispersed AuNRs and tun-able longitudinal surface plasmon resonance(LSPR)remain to be improved.herein,we report a one-pot method for synthesizing AuNRs without seeding agents.In this method,we use phenols as reducing agents and hydrochloric acid and nitric acid are used to regulate the pH of the growth solution.AuNRs with the longest LSPR peak position reaching 1340 nm are prepared.Furthermore,by systematically op-timizing concentrations of the reagents involved in the growth solution,different aspect ratios of AuNRs are synthesized.The facile synthesis,controllability aspect ratio,and long LSPR peak make our method promising for wider applications of AuNRs.

关键词： Gold nanorods Seedless method Hydroquinone Acid Aspect ratio

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

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Chinese Chemical Letters 2022年第4期33卷 1913-1916页

作者： Yuan Liu Taotao Li Gaojian Yang Yan Deng Xianbo Mou nongyue he State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Provincial Key Lab of Dark Tea and Jin-Hua School of Materials and Chemical EngineeringHunan City UniversityYiyang 413000China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Medical School of Ningbo University Ningbo 315211China

In this work, a simple gold nanoparticles(Au NPs) based colorimetric biosensor was developed for chlorpyrifos(Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant(Kd) of 58.59 ± 6.08 nmol/L was selected by ss DNA library immobilized systematic evolution of ligands by enrichment(SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for Au NPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately,thus a self-aggregation of Au NPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection.

关键词： Aptamer Colorimetric aptasensor SELEX Chlorpyrifos AuNPs

A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy

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Chinese Chemical Letters 2022年第8期33卷 4089-4095页

作者： Liming Zhang Lingfeng Xu Yi Wang Jieyu Liu Guanghong Tan Fengying Huang nongyue he Zhuoxuan Lu Key Laboratory of Tropical Translational Medicine of Ministry of Education&Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical UniversityHaikou 571199China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Clinical Laboratory HwaMei HospitalUniversity of Chinese Academy of SciencesNingbo 315000China

With an intensive understanding of the mechanism of immune system,developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer immunotherapy.In this study,we report a facile approach to prepare graphene oxide(GO)-based therapeutic cancer-nanovaccine.The model antigen(ovalbumin,OVA)and adjuvant(CpG ODN),are conjugated with GO-PEI nanosheet through electrostatic interaction.The addition of PEG can improve biocompatibility and prevent nanoparticle aggregation.The prepared GO-based nanovaccine,GO-PEI-OVA-PEG-CpG,exhibits good biocompatibility and low toxicity both in vivo and in vitro.More importantly,it can efficiently induce the maturation of dendritic cells(DCs),the enhancement of antigen cross-presentation ability,and the amplification of cytokine production of immune cells.Impressively,this nanovaccine shows a remarkable therapeutic effect against preestablished B16-OVA-melanoma tumors,which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing mice.Moreover,combining GO-PEI-OVA-PEG-CpG with NLG919,an IDO-1(indoleamine-2,3-dioxygenase)inhibitor which can regulate the tumor microenvironment,displays a synergistic therapeutic effect.These findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes.

关键词： Nanovaccine Graphene Oxide Adjuvant Tumor-specific antigen NLG919

In situ wound sprayable double-network hydrogel: Preparation and characterization

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Chinese Chemical Letters 2022年第4期33卷 1963-1969页

作者： Chenglong Cai Ting Wang Xu Han Shaoqiang Yang Chengteng Lai Tao Yuan Zhangqi Feng nongyue he State Key Laboratory of Bioelectronics National Demonstration Centre for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Southeast University Jiangbei New Area Innovation Institute Nanjing 210096China Nanjing Jinling Hosp Dept OrthopedNanjing 210002China School of Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China

In clinical settings the wound-dressing was required easy to use and can match the wound area immediately, at the same time they need to have the properties of hemostats, anti-inflammation and promoting wound healing. To get an ideal wound dressing, we developed a type of gel-like wound adhesive patch from spraying double-network hydrogel, which own the properties of self-antibacterial and can promote wound healing. By spraying, the gel-like wound adhesive patch can match the wound area immediately and form a gel-film in 10 s. Sodium carboxymethyl cellulose as p H sensitive materials accelerated the speed to form the gel-film and enhanced ductility of the wound adhesive patch. In vitro experiments show that, this gel-like wound adhesive patch can promote cell proliferation and reduce cell apoptosis.In vivo studies show that, compared with commercialized wound dressings in clinic using, the spraying gel-like wound adhesive patch from our work has a better effect on wound healing. In conclusion, the spraying gel-like wound patch in our work is easy to use and can form a gel-film match on wound area in a short time, also it has the properties of hemostats, anti-inflammation and promoting wound healing.Its feasibility for mass production shows a good potential for commercial use.

关键词： Hydrogel Sodium alginate Chitosan Spray method Sodium carboxymethyl cellulose

On-chip classification of micro-particles using laser light scattering and machine learning

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Chinese Chemical Letters 2022年第4期33卷 1885-1888页

作者： Mubashir Hussain Xiaolong Liu Jun Zou Jian Yang Zeeshan Ali Hamood Ur Rehman nongyue he Jianguo Dai Yongjun Tang Innovation Practice Shenzhen PolytechnicShenzhen 518055China School of Materials and Chemical Engineering Hunan Institute of EngineeringXiangtan 411104China Department of Electrical Engineering College of EMENational University of Sciences and TechnologyH-12 IslamabadPakistan State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

The rapid detection of microparticles exhibits a broad range of applications in the field of science and technology. The proposed method differentiates and identifies the 2 μm and 5 μm sized particles using a laser light scattering. The detection method is based on measuring forward light scattering from the particles and then classifying the acquired data using support vector machines. The device is composed of a microfluidic chip linked with photosensors and a laser device using optical fiber. Connecting the photosensors and laser device using optical fibers makes the device more diminutive in size and portable. The prepared sample containing microspheres was passed through the channel, and the surrounding photosensors measured the scattered light. The time-domain features were evaluated from the acquired scattered light, and then the SVM classifier was trained to distinguish the particle’s data. The real-time detection of the particles was performed with an overall classification accuracy of 96.06%. The optimum conditions were evaluated to detect the particles with a minimum concentration of 0.2 μg/m L. The developed system is anticipated to be helpful in developing rapid testing devices for detecting pathogens ranging between 2 μm to 10 μm.

关键词： Particle’s detection Laser light scattering Waveform features Support vector machines Lab-on-chip

Application of adaptive pressure-driven microfluidic chip in thyroid function measurement

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Chinese Chemical Letters 2021年第5期32卷 1747-1750页

作者： Xingshang Xu nongyue he State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices College of Life Sciences and ChemistryHunan University of TechnologyZhuzhou 412007China Nanjing Lanyu Biotechnology Co. Ltd.Nanjing 210000China

The improvement in accuracy of in vitro diagnosis has always been the focus of early screening of thyroid dysfunction.We constructed a microfluidic chip based on a polystyrene polymer substrate.Total triiodothyronine(TT3),total thyroxine(TT4),free triiodothyronine(FT3),free thyroxine(FT4),and thyrotropin(TSH) in human whole blood samples were analysed by fluorescence immunoassay to evaluate thyroid function.The results indicate that the microfluidic chip shows a good linear relationship in the detection of TT3,TT4,FT3,FT4,and TS H standards,and the correlation coefficient(r) is not less than0.9900.In addition,the chip also has strong anti-interference(RSD%≤5%) and good repeatability(CV≤8%),and its inter-batch differences are small(CV ≤15%).The results of practical application in clinical thyroid function mea surement indicated its high accuracy(r≥0.9900).It provides a new method for the determination of thyroid function and lays a foundation for subsequent clinical application.

关键词： Microfluidic chip Adaptive pressure drive Thyroid dysfunction In vitro diagnosis Whole blood injection

Applications of aptamers for chemistry analysis, medicine and food security

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Science China Chemistry 2015年第7期58卷 1122-1130页

作者： Rongrong Huang Zhijiang Xi nongyue he State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University College of Life Science Yangtze University

Since aptamer and its in vitro selection process called SELEX were independently described by Ellington and Gold in 1990, extensive research has been undertaken and numerous isolated aptamers for various targets have been applied. Aptamers can bind to a wide range of targets that include small organic molecules, inorganic compounds, haptens and even whole cells with high binding affinity and specificity. Aptamers for a wide range of targets have been selected currently. In addition, aptamers are thermo stable and can also be regenerated easily within a few minutes denaturation, which makes them easy to store or handle. These advantages make aptamers extremely suitable for applications based on molecular recognition as analytical, diagnostic and therapeutic tools. In this review, the recent applications of aptamers for chemistry analysis, medicine and food security, along with the future trend will be discussed.

关键词：安全应用化学分析适体医药食品 SELEX 无机化合物结合亲和力

A novel cartridge for nucleic acid extraction,amplification and detection of infectious disease pathogens with the help of magnetic nanoparticles

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中国化学快报:英文版 2023年第8期34卷 236-240页

作者： Yile Fang Yue Wang Liangxi Zhu Haoran Liu Xiangyi Su Yuan Liu Zhu Chen Hui Chen nongyue he State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province Hunan Key Laboratory of Green Chemistry and Application of Biological NanotechnologyHunan University of TechnologyZhuzhou 412007China

Nucleic acid detection(NAD)based on real-time polymerase chain reaction(real-time PCR)is gold standard for infectious disease detection.Magnetic nanoparticles(MNPs)are widely used for nucleic acid extraction(NAE)because of their excellent properties.Microfluidic technology makes automated NAD possible.However,most of the NAD microfluidic chips are too complex to be applied to point-of-care(POC)testing.In this paper,a simple-structure cartridge was developed for POC detection of infectious diseases.This self-contained cartridge can be divided into a magnetic-controlled NAE part,a valve-piston combined fluidic control part and a PCR chip,which is able to extract nucleic acid from up to 500μL of liquid samples by MNPs and finish the detection process from“sample in”to“answer out”automatically.Performance tests of the cartridges show that it met the demands of automated NAD.Results of on-cartridge detection of hepatitis B virus(HBV)demonstrated that this system has good uniformity and no cross-contamination between different cartridges,and the limit of detection(LOD)of this system for HBV in serum is 50 IU/mL.Multiplex detections of severe acute respiratory syndrome coronaviruses 2(SARS-CoV-2)with a concentration of 500 copies/mL were carried out on the system and 100%positive detection rate was achieved.

关键词： Nucleic acid detection Cartridge Magnetic nanoparticles Fluidic control Point-of-care testing

Highly sensitive smartphone-based detection of Listeria monocytogenes using SYTO9

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Chinese Chemical Letters 2022年第4期33卷 1933-1935页

作者： Sha Liu Xuliang he Tao Zhang Kaixuan Zhao Changhu Xiao Zengrui Tong Lian Jin nongyue he Yan Deng Song Li Yuan Guo Zhu Chen Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Department of General Surgery People’s Hospital of Zhuzhou City Affiliated to Changsha Medical CollegeZhuzhou 412011China Department of Laboratory Central Hospital of Zhuzhou CityZhuzhou 412007China State Key Laboratory of Bioelectronics School of Biological and Medical EngineeringSoutheast UniversityNanjing 210096China National&Local Joint Engineering Research Center for Research and Technology of Advanced Packaging Materials Zhuzhou 412007China

Listeriosis is caused by Listeria monocytogenes(LM) and is currently considered to be one of the leading food-borne diseases worldwide, with mortality rate of 20%~30%. Currently, detection methods for LM are time-consuming with low sensitivity, and delayed detection results. SYTO9 has a high affinity for DNA and exhibits enhanced fluorescence upon binding. Therefore, this study used SYTO9 staining and image processing to develop a rapid loop mediated isothermal amplification(LAMP) detection method for LM. Smartphone was successfully used for detecting the color change in different concentrations of LM. Besides, the optimized LAMP reaction temperature was 63 °C by color identification, and the limit of detection for LM was 6 copies/μL in the green channel. So, the developed method, based on image processing, is simple, sensitive and rapid, which provides a new idea and method for rapid detection of LM and other food-borne bacterial pathogens.

关键词： Literia monocytogenes Image process SYTO9 Loop mediated isothermal amplification Smartphone