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Immunocytochemica. identifica.ion a.d distribution of the cell types in the pituita.y gla.d of Ba.rus ba.a.(Teleostei, Ba.rida.)

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World Journa. of Neuroscience 2012年第1期2卷 23-31页

作者： Mosta.a.a. Mousa.noha a. khalil a.a. M. a.a. M. Ha.hem Fish Reproduction La.ora.ory National Institute of Oceanography and Fisheries Alexandria Egypt Zoology Depa.tment Faculty of Science Zagazig University Zagazig Egypt

Immunocytochemica. identifica.ion of the different cell types in the pituita.y gla.d of Ba.rus ba.a. wa. performed using a.tisera.a.a.nst ma.ma.ia. (huma. a.d ra.) a.d piscine hormones. The a.enohypophysis wa. composed of rostra. pa.s dista.is (RPD), proxima. pa.s dista.is (PPD) a.d pa.s intermedia.(PI). Prola.tin a.d a.renocorticotrophic cells were loca.ed in the rostra. pa.s dista.is of the pituita.y. Gona.otrophic a.d growth hormone cells were distributed in the proxima. pa.s dista.is, but gona.otrophic cells a.pea. a.so a. the border of the pa.s intermedia. Soma.ola.tin cells, a. well a. a.pha.mela.otrophic cells were loca.ed in the pa.s intermedia.of B. ba.a. pitui-ta.y. The prola.tin (PRL) cells were distributed in the RPD sta.ned with ora.ge G a.d showed strong immunorea.tivity with a.tiserum to chum sa.mon. The a.renocorticotrophic (a.TH) cells were lea. hema.toxylin-positive (PbH+) a.d showed strong im- mu-norea.tivity with a.ti-huma. a.TH;these cells bor-dered the neurohypophysis a.d grouped in islets be-tween PRL cells in the RPD. Growth hormone (GH) cells were densely distributed with the gona.otrophic (GTH) cells in the PPD. They were ora.ge G positive a.d rea.ted with a.tiserum to chum sa.mon. GTH cells were loca.ed in the centra. a.ea.of the PPD a.d in the externa. border of the PI. These cells were a.cya. Blue a.d Pa. positive, a.d immunosta.ned with a.ti-chum sa.mon GTH Iβ a.d a.ti-chum sa.mon GTH IIβ. In a.dition, a.tiserum to ra. thyrotropin stimula.ing hormone β (TSHβ) rea.ted positively to the GTH cells. These results suggest tha. GTH I, GTH II a.d TSH a.e synthesized in the sa.e cells in the pituita.y of B. ba.a.. The PI wa. composed ma.nly of PbH+ cells a.d a.Pa.+ cell a.ja.ent to the neurohypophysis. The Pa.+ cells from the PI bound specifica.ly to a.ti-chum soma.ola.tin. a.ti-a.pha. mela.in stimula.ing hormone (MSH) sta.ned only the PbH+ (a.pha.mela.otrophic) cells of the PI.

关键词： Immunocytochemistry Pituita.y Gla.d Ba.rus Ba.a. (Teleostei)

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The effect of ma.erna. thyroxine injection on growth, surviva. a.d development of the digestive system of Nile tila.ia. Oreochromis niloticus, la.va.

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a.va.ces in Bioscience a.d Biotechnology 2011年第5期2卷 320-329页

作者： noha a. khalil Ha.sa. M. M. Kha.a. a.la. Mosta.a.a. Mousa 不详

The present study dea.s with the effect of exogenous trea.ment of O. niloticus fema.es with L-thyroxine (T4) on the development of the digestive system during la.va. rea.ing, a.d its subsequent effect on la.va. growth a.d surviva.. The development of the digestive tra.t a.d a.cessory gla.ds wa. investiga.ed histologica.ly a.d histochemica.ly in the developing O. niloticus la.va., from control a.d T4-trea.ed spa.ners. During yolk-sa. a.sorption, the digestive system of the fish underwent further differentia.ion a.d the rudimenta.y a.imenta.y ca.a. beca.e segmented into four different histologica. regions: the buccopha.ynx, oesopha.us, stoma.h a.d intestine. The injection of fema.es O. niloticus with thyroxine (1 or 10 μg T4/g BW) grea.ly enha.ced the development of the digestive tra.t a.d a.cessory gla.ds of la.va. a. indica.ed by the qua.tita.ive a.d qua.ita.ive cha.ges of the mucus composition from predomina.tly neutra. to a.mixture of neutra. a.d a.id mucosubsta.ces, or a.id mucosubsta.ces occurred during the rea.ing period for the la.va. produced from T4-trea.ed fema.es. This ma. be due to the direct effect of exogenous thyroxine, which might ha.e been tra.sferred from ma.erna. circula.ion into the oocytes a.d la.va., on the synthesis of proteins, which increa.ed with la.va. development. Thus, thyroxine directly or indirectly improved O. niloticus la.va. growth, since a.ma.ked increa.e in both, length a.d weight of la.va. occurred during the experimenta. period. In a.dition, la.va. from trea.ed fema.es a.so ga.e a.significa.tly higher surviva. ra.e tha. tha. of control. It could be concluded tha. exogenous T4 in ma.erna. circula.ion might ha.e been tra.sferred into oocytes a.d la.va.. The tra.sferred thyroid hormone a.pea.s to pla. some role in the ea.ly development of la.va. a.d ma. confer a.distinct a.va.ta.e for the growth of the offspring of the Nile tila.ia. O. niloticus.

关键词： Fish La.va. O. Niloticus Thyroxine Growth Digestive System

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Ground Ma.netic, GPR, a.d Dipole-Dipole Resistivity for La.dfill Investiga.ion

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Interna.iona. Journa. of Geosciences 2016年第6期7卷 828-848页

作者： Moha.ed H. khalil noha a. Ha.sa. Geophysics Depa.tment Faculty of Science Cairo University Giza Egypt

Ground ma.netic, ground penetra.ing ra.a. (GPR), a.d dipole-dipole resistivity were ca.ried out to environmenta.ly investiga.e a.la.dfill. In this context, these geophysica. techniques were conducted to identify the subsurfa.e contents of the la.dfill, furthermore, specify a.y possible lea.a.e a.d/or conta.ina.ion in the study a.ea. The ground-ma.netic survey ca.ried out in the study a.ea.comprised 31 profiles ea.h 120 m in length. Different wa.elength filters were a.plied to the mea.ured da.a. Vertica. deriva.ive, downwa.d continua.ion, a.pa.ent susceptibility, ba.d-pa.s, a.d a.a.ytica. signa. filters sepa.a.ed successfully the sha.low sources. Wherea., upwa.d continua.ion a.d low-pa.s Ga.ssia. filters isola.ed significa.tly the deep ma.netic sources. 3D Euler deconvolution (SI = 3) rema.ka.ly estima.ed the depths of the sha.low sources (0 - 10 m) of the la.dfill contents. The conducted GPR a.d dipole-dipole resistivity a.loca.ed ta.gibly the loca.ions a.d depths of the nea. surfa.e a.oma.ies. Both techniques didn’t revea. a.y possible lea.a.e a.d/or conta.ina.ion. Noteworthy, integra.ion a.ong ma.netic, GPR, a.d dipole-dipole resistivity confirmed positively the results of ea.h method. Nevertheless, some a.oma.ies were recognized successfully by one technique a.d not by the others.

关键词： La.dfill Investiga.ion Ground Ma.netic GPR Electric Profiling Vertica. Deriva.ives Continua.ion Filter Integra.ed Techniques

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前段光学相干断层扫描在穿透性角膜移植术后移植上皮变化中的应用（英文）

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国际眼科杂志 2020年第3期20卷 410-414页

作者： Sha.ma. a.a.feen Riha. SHM a.la. noha M khalil Gha.a.a.Na.sa. 开罗大学Ka.r a. a.ny医学院眼科

目的:使用前段光学相干断层扫描(a.-OCT)评估在穿透性角膜移植术(PK)后角膜上皮厚度的变化,并确定上皮厚度在移植排斥反应早期检测中的作用。方法:前瞻性比较观察研究。共纳入20例20眼接受穿透性角膜移植术患者做为研究组,对照组16例16... 详细信息

目的:使用前段光学相干断层扫描(a.-OCT)评估在穿透性角膜移植术(PK)后角膜上皮厚度的变化,并确定上皮厚度在移植排斥反应早期检测中的作用。方法:前瞻性比较观察研究。共纳入20例20眼接受穿透性角膜移植术患者做为研究组,对照组16例16眼。术后2wk、1mo和3mo使用a.-OCT测量角膜厚度。术后3mo,与对照组排斥反应和上皮厚度及分布参数进行比较。结果:术后1mo,研究组的上、下、最大和最小上皮厚度值均显著低于术后2wk(P=0.0004,0.0001,0.0001,0.04),术后3mo与1mo相比无显著差异(P=0.4,0.1,0.8)。与术后2wk相比,1mo时上皮厚度的降低明显高于基质厚度(P=0.04)。角膜上皮厚度变化显示,研究组和对照组的角膜上皮厚度分布模式相似,出现明显的角膜水肿。但是,对照组中央角膜厚度(CCT)明显更高。结论:角膜移植术后移植物上皮重塑的定量评估显示,早期变化有助于角膜移植物厚度的显著改善。角膜上皮厚度和分布模式的变化可作为角膜移植排斥反应的指标。

关键词： a.-OCT 角膜上皮厚度穿透性角膜移植术上皮重塑角膜移植排斥

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