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Relative Viability in Drosophila melanogaster in“Evolution Canyon”,Israel

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Journal of Life Sciences 2018年第4期12卷 169-172页

作者： Misato Uchida Eviatar Nevo nobuhiko asada Department of Zoology Faculty of ScienceOkayama University of ScienceKita-kuOkayama 700-0005Japan Institute of Evolution Faculty of Science and EducationUniversity of HaifaMount CarmelHaifa 31905Israel Graduate School of Biology Okayama UniversityKita-kuOkayama 700-8530Japan

Genetic diversity in natural populations has been explored through evolutionary analysis,and patterns of microscale biodiversity have been studied in populations at“Evolution Canyon”,Israel.A total of 2,000 eggs from each of iso-female Drosophila melanogaster line 2-1 from the north-facing slope(NFS)and line 6-1 from the south-facing slope(SFS)were placed in 20 vials and evaluated.Egg-to-adult viability was 0.89 and 0.75 in lines 2-1 and 6-1,respectively,and the difference was statistically significant by t-test.Genetic diversity was higher on the harsher SFS than on the milder NFS.Inter-slope genetic differences corroborated the effect of stochastic climate selection pressures to viability polygenes in populations at“Evolution Canyon”,Israel.

关键词： Viability variability Drosophila melanogaster “Evolution Canyon”

Stability and Refolding of Prophenol Oxidase Protein with 2-Propanol in Drosophila melanogaster

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Journal of Life Sciences 2012年第8期6卷 952-956页

作者： Eri Sato Kotomi Mita nobuhiko asada Department of Zoology Faculty of Science Okayama University of Science Kita-ku Okayama 700-0005 Japan Graduate School of Systems Life Sciences Kyusyu University Higashi-ku Fukuoka 812-8581 Japan

Phenol oxidase in Drosophila melanogaster occurs as folded phase precursors designated as prophenol oxidase A1 and A3, and prophenol oxidase is activated with alcohol, especially 2-propanol, within a few minutes as unfolded-phase in vitro. To clarify a common effect of alcohols on proteins and peptides, the extract containing prophenol oxidase protein was prepared. Phenol oxidase activity activated with 2-propanol has been maintained stable at least 24 hours remains as it is. Protein of prophenol oxidase was not denatured opposite hypnoses known as the instability of protein with alcohol. Activated prophenol oxidase with 2-propanol remain enzyme activity with no aggregation, stable, renaturation, and the refolding phenomena occurred around the active phase within the catalytic active center of prophenol oxidase protein in Drosophila melanogaster. This study is important to induce the wide range applications of the effect in many fields for rational drag design.

关键词：酚氧化酶不稳定性黑腹果蝇酶蛋白丙醇复性催化活性中心蛋白质

Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes

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Journal of Life Sciences 2014年第6期8卷 517-521页

作者： nobuhiko asada Aoi Kedamori Yumiko Kusano Tetsuro Takeuchi Department of Zoology Faculty of Science Okayama University of Science Kita-ku Okayama 700-0005 Japan Faculty of Humanities Shujitsu University Higashi-ku Okayama 703-8255 Japan

BdlR strain killifish Oryzias latipes, which can be found in natural populations in Kochi Prefecture, Japan, exhibit a fading body-color. The aim of this article is trace of body fade by enzymatic analysis especially blacking key enzyme tyrosinase. A single scale was collected from a single specimen of this strain at the dorsal axis, and the supernatant fraction developed from this sample served as a tyrosinase extract. The extract fraction showed low tyrosinase activity when L-tyrosine, L-dopa and L-dopamine were used as enzyme substrates. After addition of L-cysteine to the sample scale, surface color gradually faded as pheomelanin formed. Tyrosinase was believed to have been in the folded phase and especially active around the catalytic site and copper was detected in the scale. In hereafter, molecular analysis can attain above purpose.

关键词：酪氨酸酶活性表面颜色青鳉应变黑素变异机身衰落

Molecular Evolution of the Cytochrome b Gene in Geographically Isolated Populations of Oryzias Latipes

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Journal of Life Sciences 2011年第4期5卷 282-286页

作者： nobuhiko asada Akira Hirai Taiki Hamada Eri Sato Akio Moriyama Tetsuro Takeuchi Department of Zoology Faculty of Science Okayama University of Science Kita Okayama 700-0005 Japan Misaki-cho Okayama 716-1122 Japan Hamada 3rd Junior High School Hamada 695-0101 Japan Faculty of Humanities Shujitsu University Higashi Okayama 703-8255 Japan

The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5’ and 3’ ends was 1,143 base pairs (bp) and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control (Hd-rR strain) and HS strains than the K-11 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 (K-11 strain) and AB480879 (HS strain).

关键词：线粒体细胞色素b基因分子进化 GenBank数据库氨基酸残基人群隔绝地理核苷酸序列

Microevolution of Mitochondrial Cytochrome oxidase subunit I in Drosophila melanogaster at ＂Evolution Canyon＂, Israel

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Journal of Life Sciences 2015年第10期9卷 457-464页

作者： nobuhiko asada Hui Sun Kaori Hayashi Kohta Inomata Yu Harada Erika Sugino Shintaro Takasaki Eviatar Nevo Department of Zoology Faculty of Science Okayama University of Science Kita-ku Okayama 700-0005 Japan School of Life Science Northeast Normal University Renmin Street Changchun 130024 China Institute of Evolution Faculty of Science and Education University of Haifa Mount Carmel Haifa 31905 Israel

We determined the sequence of mitochondrial Cytochrome oxidase subunit I (CO1) in two Drosophila melanogaster strains originating at "Evolution Canyon", Israel. CO1 nucleotide sequences from two iso-female strains, 2-1 and 6-1, were 1,534 and 1,543 base-pairs, respectively. In each strain, ATAA was used in initiation of translation. Exchange rates for nucleotide and amino acid sequences were larger in the 6-1 strain than in the 2-1 strain when Oregon-R was used as the standard. Non-synonymous exchange rate was larger than synonymous exchange rate among the three strains.

关键词：细胞色素氧化酶线粒体以色列进化峡谷果蝇亚基核苷酸序列

Molecular mechanisms of lesion-induced axonal sprouting in the corticofugal projection:the role of glial cells

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Neural Regeneration Research 2023年第6期18卷 1259-1260页

作者： Leechung Chang nobuhiko Yamamoto Laboratory of Cellular and Molecular Neurobiology Graduate School of Frontier BiosciencesOsaka UniversitySuitaOsakaJapan

After injury of the central nervous system(CNS),neuronal circuits are known to remodel for functional recovery.In general,there are two strategies for the remodeling:axonal regeneration and sprouting(Figure 1A).Axonal regeneration is re-growth of injured neurons themselves,but axons hardly regenerate in the adult mammalian CNS(Silver and Miller,2004).On the other hand,axonal sprouting is new growth from intact or spared neurons to the denervated target by forming axon collaterals,compensating for damaged circuits.This process is a more effective way for circuit remodeling,as it does not necessarily require long axonal elongation.

关键词： axonal projection Figure

Safety and feasibility of laparoscopic pancreatoduodenectomy

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Hepatobiliary Surgery and Nutrition 2022年第2期11卷 330-332页

作者： nobuhiko Nakagawa Yuichi Nagakawa Shingo Kozono Department of Gastrointestinal and Pediatric Surgery Tokyo Medical UniversityTokyoJapan

Laparoscopic pancreaticoduodenectomy(LPD)has been safely performed by experts with favorable surgical outcomes.However,the feasibility of LPD remains controversial.LPD is generally considered a challenging operation that requires advanced laparoscopic techniques.In particular,pancreatic reconstruction can be safely performed by only laparoscopic experts.Thus,guidelines created by LPD experts are valuable in indicating the future direction of LPD.Qin et al.developed practical guidelines regarding the safety and feasibility of LPD with 28 international experts from eight countries(1).To create these guidelines,the first summit on minimally invasive pancreatico-biliary surgery was held in Wuhan,China,to promote the development of LPD.Based on a systematic literature review and expert opinions,16 statements were created by the experts.These statements provide useful guidelines not only for surgical trainees who will attempt LPD but also for specialized surgeons.This expert consensus will contribute to the safe implementation of LPDs in the future.

关键词： laparoscopic specialized safely

Circuits and devices for standalone large-scale integration (LSI) chips and Internet of Things (IoT) applications: a review

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Chip 2023年第3期2卷 7-19页

作者： Takaya Sugiura Kenta Yamamura Yuta Watanabe Shiun Yamakiri nobuhiko Nakano Department of Electronics and Electrical Engineering Keio UniversityYoko-hamaKanawaga223-8522Japan

In recent years,Internet of Things(IoT)has become more and more important owing to the rapid expansion of the number of computing devices and data sizes.The evolution of IoT requires low-power and self-operating devices to expand the coverage area of computing re-sources.The main components of IoT are the large-scale integration(LSI)chips,which take the function of implementing the energy har-vesters,control units and applications.They exhibit different physics or phenomena,making it difficult to understand and design the entire system.The current work reviews the various methods for IoT appli-cations by CMOS LSI chips,from the power components by energy harvesting to realistic applications with future outlooks.

关键词： Analog LSI Energy har vesting Inter net of Things Sensors

Active Control of Fan Noise

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Journal of Thermal Science 2008年第2期17卷 163-169页

作者： nobuhiko YAMASAKI Hirotoshi TAJIMA Department of Aeronautics and Astronautics Kyushu University

在醒来转子相互作用扇子噪音，很多在传递频率(BPF ) 和它的泛音的片的交往的模式被产生它被定子和转子片等等的数字规定。在现在的学习，主导的模式被试试被第二等的声音从扬声器致动器压制。现在的系统的新奇特征之一是有领域可编程... 详细信息

在醒来转子相互作用扇子噪音，很多在传递频率(BPF ) 和它的泛音的片的交往的模式被产生它被定子和转子片等等的数字规定。在现在的学习，主导的模式被试试被第二等的声音从扬声器致动器压制。现在的系统的新奇特征之一是有领域可编程的门数组(FPGA ) 硬件和 LabVIEW 软件的控制板的采纳同步有在任意的扇子下面的旋转的片的相对地点的环绕地安装的扬声器致动器旋转速度。实验在 2004， 3150，和 4002 的三旋转速度的条件下面被进行[rpm ] 。在健全压力水平(系统程序设计语言) 的减小为所有三旋转速度被观察。在 BPF 的健全压力水平被减少约 13 [dB ] 为 2004 [rpm ] 大小写。

关键词：定子-转子系统技术性能抗噪音控制系统切割技术