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Isolation and characterization of a profenofos degrading bacterium

Journal of Environmental Sciences 2009年第11期21卷 1591-1597页

作者： Saadatullah Malghani nivedita chatterjee Key Laboratory of Biogeology and Environmental Geology Ministry of Education of China School of Environmental StudiesChina University of Geosciences

Profenofos, a well known organophosphate pesticide, has been in agricultural use over the last two decades for controlling Lepidopteron pests of cotton and tobacco crops. In this study, a bacterial strain, OW, was isolated from a long term profenofos exposed soil by an enrichment technique, and its ability to degrade profenofos was determined using gas chromatography. The isolated strain OW was identified as Pseudomonas aeruginosa according to its physiological and biochemical properties, and the analysis of its 16S rRNA gene sequence. The strain grew well at pH 5.5-7.2 with a broad temperature profile. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 μg/g profenofos, which resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium （FTW）, removal in the level of profenofos of 86.81% was obtained within 48 h of incubation. The intermediates of profenofos metabolism indicated that the degradation occurred through a hydrolysis mechanism, and one of the metabolites was found to be 4 bromo-2-cholorophenol （BCP） which in turn was also mineralized by the strain. The results of this study highlighted the potentiality of P. aeruginosa as a biodegrader which could be used for the bioremediation of profenofos contaminated soil.

关键词： profenofos organophosphate pesticide Pseudomonas aeruginosa

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Exposure-response of Cr(Ⅲ)-organic complexes to Saccharomyces cerevisiae

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Frontiers of Environmental Science & Engineering 2010年第2期4卷 196-202页

作者： nivedita chatterjee Zejiao LUO Key Laboratory of Biogeology and Environmental Geology Ministry of Education(BGEG)School of Environmental StudiesChina University of GeosciencesWuhan 430074China

Hexavalent chromium[Cr(VI)]bioreduction produces soluble Cr(Ⅲ)-organic *** Cr(Ⅲ)-organic complexes are relatively stable once they are formed,and no data about their toxicity were ***,this study aims to investigate the bioavailability and toxicity of the soluble Cr(Ⅲ)-organic *** cerevisiae L^(-1)wild type yeast strain was chosen as the model organism and Cr(Ⅲ)-citrate was selected as the representative compound of the Cr(Ⅲ)-organic *** short-term chronic aquatic toxicity tests of the Cr(Ⅲ)-citrate was explored by measuring growth inhibition,direct viable cell count,dry biomass,biosorption,and the amount of CO_(2)***(Ⅲ)-citrate exerted a toxicity of 51 mg/L with an EC_(50),which was calculated from the percent growth *** toxicity data would be helpful to define the toxic potential of the organo-chromium-Ⅲcompounds in the environment.

关键词： Cr(Ⅲ)-organic complexes Saccharomyces cerevisiae toxicity EC_(50) bioavailability

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