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Molecular Cloning of clpX Gene from Vibrio alginolyticus hY9901 and Its Bioinformatics Analysis

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Asian Agricultural Research 2024年第8期16卷 17-22页

作者： Xiaoxin WEN Yuyan hE Jiajie MA Weijie ZhANG huanying PANG na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524088China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong higher Education Institutes Zhanjiang 524088China Chinese Academy of Inspection and Quarantine Beijing 100176China

According to the clpX gene sequence of Vibrio alginolyticus hY9901,a pair of specific primers were designed,and the full length was cloned by PCR and subjected to bioinformatics *** results showed that the clpX gene was 1281 bp in length and encoded 426 amino *** molecular structure formula was C 3842 h 6405 N 1281 O 1598 S 260,with a theoretical protein molecular weight of approximately 1044473.4 kDa and a theoretical pI value of *** clpX gene was predominantly situated within the cytoplasm,exhibiting unstable and hydrophilic protein *** possessed a signal peptide cleavage site,lacked a transmembrane region,and was not associated with any KEGG metabolic ***,it possessed 2 glycine phosphorylation sites,a CAMP-dependent protein kinase phosphorylation site,a C-terminal amidation modification site,6 protein kinase C phosphorylation sites,7 microbody C-terminal target signal sites,and an ATP/GTP *** clpX phylogenetic tree was constructed using the MEGA 5.0 software via the neighbor-joining *** results demonstrated that the clpX of *** exhibited up to 100%affinity with the clpX of Vibrio *** single subunit 3D structure model of the ClpX protein was obtained using the SWISS-MODEL program.A structural and functional analysis of the protein revealed the presence of three distinct ClpX structural and functional *** the prediction of secondary structure,the proportions ofα-helix,random coil,β-sheet and extended strand were 40.38%,37.09%,5.40%and 17.14%,*** analysis of the ClpX protein through the STRING database revealed that the proteins interacting with the ClpX protein were Tig,Atpd,hflb,Msrb-2,Rpod,Clpp,Clpa,Lon-1,hfq,and ANP63951.1.A computational analysis of the ClpX protein identified a number of post-translational modification sites,including phosphorylation,acetylation,ubiquitination,glycosylation,methylation,S-palmitoylation,and *** significance of this st

关键词： Vibrio alginolyticus clpX gene Bioinformatics analysis

The Bright Single Pulse Emission from PSR B1133+16

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Research in Astronomy and Astrophysics 2024年第4期24卷 253-261页

作者： Jun Tan Zhi-Gang Wen Zhen Wang Xue-Feng Duan hong-Guang Wang na Wang Vishal Gajjar Jian-Ping Yuan Rai Yuen Wen-Ming Yan Jian-Ling Chen Wei han hui Wang Cheng-Bing Lyu Ai-Jun Dong Maths and Information Technology School Yuncheng UniversityYuncheng 044000China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China Xinjiang Key Laboratory of Radio Astrophysics Urumqi 830011China School of Physics and Materials Science Guangzhou UniversityGuangzhou 510006China Space Science Laboratory University of CaliforniaBerkeley 94710USA Department of Physics and Electronic Engineering Yuncheng UniversityYuncheng 044000China School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550025China

We have conducted a comprehensive investigation into the bright single pulse emission from PSR B1133+16using the Giant Metrewave Radio *** time resolution data(61μs)were obtained at a center frequency of 322 Mhz with a bandwidth of 32 Mhz over a continuous observation period of 7.45 hr.A total of 1082 bright pulses were sporadically detected with peak flux densities ranging from 10 to 23 times stronger than the average pulse ***,no giant pulse-like emission with a relative pulse energy larger than 10 and extremely short duration was detected,indicating that these bright pulses cannot be categorized as giant pulse *** majority of these bright pulses are concentrated in pulse phases at both the leading and trailing windows of the average pulse profile,with an occurrence ratio of approximately *** pulse energy distribution for all individual pulses can be described by a combination of two Gaussian components and a cutoff power-law with an index of α=-*** updated nulling fraction of 15.35%±0.45% was determined from the energy *** emission of individual pulses follows a log-normal distribution in peak flux density *** is imperative that regular phase drifting in bright pulse sequence is identified in both the leading and trailing components for the first *** physical mechanisms are discussed in detail to provide insights into these observations.

关键词： (stars:)pulsars:general (stars:)pulsars:individual(PSR B1133+16) methods:observational methods:data analysis

Epidemiological, Clinical, Therapeutic, and Evolutionary Aspects of Chronic End-Stage Renal Failure in the Nephrology Department of the University hospital of Brazzaville in 2023

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Open Journal of Nephrology 2024年第1期14卷 62-69页

作者： Gaël honal Mahoungou Daniel Tony Eyeni Sinomono Éric Ngandzali-Ngabé Soraya Ntandou Francky Ambounou Précieux Ngoma Berline Ndinga Dalhia Mboungou héléna Botokoto Bothard hardy Ibovi Richard Loumingou Faculty of health Sciences Marien Ngouabi University Brazzaville Congo Department of Nephrology University Hospital of Brazzaville Brazzaville Congo

Objective: Chronic end-stage renal failure is a major public health problem in developing countries and is poorly documented. The objective of this study was to describe the epidemiological, diagnostic, therapeutic, and evolutionary aspects of patients admitted for end-stage renal failure to the Department of Nephrology at the University hospital of Brazzaville. Patients and Methods: This was a descriptive cross-sectional study collecting prospective data that took place over 10 months. We included 128 patients who were exhaustively identified. Sociodemographic, clinical, therapeutic and developmental data were collected using a standardized and pretested pre-established survey form. The EPI info software enabled the analysis and processing of the data. Results: We reported a male predominance with a sex ratio of 2.5. The median age was 50 years. The first cause found was diabetes (55%) followed by hypertension (46%). The majority of patients had a dialysis emergency and half could not be dialyzed for financial reasons. Conclusion: Our results highlighted that certain epidemiological parameters of end-stage renal failure are constantly changing, such as frequency, while others remain static, including the main etiologies, gender, and age. however, mortality continues to increase and deserves more attention.

关键词： ESRD Epidemiology Nephrology University hospital of Brazzaville

Incidence and Risk Factors of Sub-syndromal Delirium in Patients after Cardiac Surgery

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Medicinal Plant 2024年第1期15卷 62-65,78页

作者： Baoyi YANG Longti LI na WANG Yan ZhAN Lei CAO Rong WANG Department of Intensive Care Unit Taihe HospitalHubei University of MedicineShiyan 442000China

[Objectives]This study aimed to investigate the incidence and risk factors associated with SSD in patients following cardiac surgery.[Methods]A total of 378 patients who underwent cardiac surgery in Taihe hospital were recruited and *** of delirium was made using evaluation methods and DSM-5 *** was defined as the presence of one or more core features of delirium without meeting the full diagnostic *** analysis included independent samples t-test for group comparisons and binary logistic regression analysis to identify independent risk factors for SSD after cardiac surgery.[Results]Among the 378 subjects,112(29.63%)had SSD,28(7.41%)had delirium,and the remaining 238 patients(62.96%)did not present with *** analysis revealed that age,APAChE II score,duration of aortic clamping,length of ICU stay,duration of sedation use,and daily sleep time were significant risk factors for the occurrence of SSD(Pnalysis identified age>70 years old,APAChE II score>20 points,length of ICU stay>5 d,and duration of sedation use>24 h as independent risk factors for SSD after cardiac surgery(Pnal model was fitted based on the analysis results of the binary logistic regression model,yielding the equation logit P=1.472X_(1)+2.213X_(2)+3.028X_(3)+1.306X_(4).[Conclusions]Comprehensive clinical assessment is crucial for patients undergoing cardiac surgery,and appropriate preventive measures should be taken for patients with identified risk *** monitoring of the patient s consciousness should be implemented postoperatively,and timely interventions should be *** research should focus on model validation and optimization.

关键词： Subsyndromal delirium Cardiac surgery Patient Confusion assessment method for intensive care units version(CAM-ICU)

Molecular Cloning and Bioinformatics Analysis of cyaA Gene of Vibrio alginolyticus Strain hY9901

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Agricultural Biotechnology 2024年第4期13卷 6-10页

作者： Shi WANG Peng ZhOU Yujia ZhANG Weijie ZhANG na WANG huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to explore the biological functions of cyaA gene of Vibrio alginolyticus.[Methods]With Dna of *** hY 9901 as a template,primers were designed according to the sequence of cyaA gene,and the cyaA gene was amplified by *** analysis was performed.[Results]The cyaA gene of *** hY9901 was 2529 bp in size,and encoded 842 amino *** molecular structure of CyaA protein was C_(4358)h_(6745)N_(1171)O_(1286)S_(35).Its theoretical molecular weight was 97.24167 kDa and the theoretical pI value was *** had no signal peptide and transmembrane *** protein had three N-terminal glycosylation sites,one cAMP and cGMP-dependent protein kinase phosphorylation site,nine protein kinase C phosphorylation sites,nine casein kinase II phosphorylation sites,one tyrosine kinase phosphorylation site,seven N-terminal myristoylation sites,one pentenyl binding site and ten microbody C-terminal localization signal *** localization prediction showed that CyaA protein was mainly located in the nucleus and *** multi-sequence alignment and phylogenetic tree construction,it was concluded that *** had high CyaA homology with other Vibrio *** of *** was clustered with Vibrio fluminensis and Vibrio marinisedimini,and they were closely *** secondary structure of CyaA protein consisted ofα-helixes(43.11%),random coils(38.00%)and extended strands(14.49%).In protein network interaction,it was found that the proteins adjacent to CyaA protein were Crp-2,CpdA,Crr,PtsG-2,ANP67209.1,Crp-1,PykF,Pyk,RelA and Ndk.[Conclusions]This study provides a new idea for formulating strategies for the prevention and control of vibriosis.

关键词： Vibrio alginolyticus cyaA gene Bioinformatics analysis

First complete genomic sequence analysis of canine distemper virus in wild boar

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Virologica Sinica 2024年第4期39卷 702-704页

作者： Tong Wang haiying Du na Feng Yuhang Liu Yu Xu heting Sun Peng Peng Siyuan Qin Xiaotian Zhang Yan Liu Mingyuan Yu hongrui Liang Biao he Guoqiang Zhu Changchun Tu Zhongzhong Tu Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou UniversityYangzhou 225009China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun 130122China Biological Disaster Control and Prevention Center National Forestry and Grassland AdministrationShenyang 110034China

Dear Editor,Canine distemper virus(CDV)is the causal agent of a highly contagious viral infectious disease that affects domestic and wild carnivores *** is an enveloped,non-segmented negative sense Rna virus that belongs to the Morbillivirus genus in Paramyxoviridae family,which contains viruses of epidemiological relevance to humans and *** on the variability of the hemagglutinin gene(h),CDV strains have been at least 21 major genetic lineages:America-1 to America-5,Canada-1 and-2,Asia-1 to Asia-6,Europe wild-life,Arctic,Africa-1/South Africa,Africa-2,South America-1 to South America-3 and Rockborn-like(Giacinti et al.,2022).Mutations in the binding sites of the h protein,which interact with viral entry receptors such as signaling lymphocytic activation molecule(SLAM)and nectin-4,are associated with the emergence of the disease in new host species(Beineke et al.,2015).

关键词： distemper Canada globally

Molecular Cloning of sodB Gene from Vibrio alginolyticus hY9901 and Its Bioinformatics Analysis

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Asian Agricultural Research 2024年第7期16卷 42-47页

作者： Shuai YANG Yingying JIANG haiyun FENG Weijie ZhANG na WANG Xiaonan LU huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing100176China Department of Food Science and Agricultural Chemistry Faculty of Agricultural and Environmental SciencesMcGill UniversitySainte-Anne-de-Bellevue H9X 3V9Canada

Vibrio alginolyticus is a zoonotic bacterium.A pair of specific primers was designed using the sodB gene sequence of Vibrio alginolyticus hY9901 in order to amplify the full length of the gene by *** results indicated that the total length of the sodB gene was 585 bp and that it could encode 194 amino *** predicted amino acid sequence derivation indicated that the molecular weight of the protein was approximately 21.56 kDa,with an isoelectric point of *** prediction of the N-terminal signal peptide structure of the protein,no significant signal peptide cleavage site was observed,indicating that the protein lacked both a signal peptide and a transmembrane *** amino acid sequence contained an N-glycosylation site,a casein kinase II phosphorylation site,a microsomal C-terminal target signal site,and a manganese and iron superoxide dismutase signal *** probability of intracytoplasmic localization of the SodB protein was 56.5%,which was analyzed according to the subcellular localization of the *** amino acid sequence of the sodB gene of *** exhibited 98%-100%homology to other Vibrio species,clustering into the same subfamily with ***,indicating a relatively close relationship between *** the prediction of protein structure,the proportions ofα-helix,random coil,β-sheet,and extended strand were 48.45%,30.41%,5.67%,and 15.46%,*** similarity to template 1dt0.1.A reached 71.58%.A PTM site analysis revealed the presence of phosphorylation,glycosylation,ubiquitination,sumoylation,acetylation,and methylation modification sites,as well as the absence of lactylation modification sites.

关键词： Vibrio alginolyticus Gene cloning sodB gene Bioinformatics analysis

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电化学二电子氧还原制备过氧化氢研究进展

物理化学学报 2024年第2期40卷 1-11页

作者：文兆宇韩娜李彦光苏州大学功能纳米与软物质研究院江苏苏州215123 澳门科技大学材料科学与工程研究院中国澳门氹仔岛999078

利用电化学二电子氧还原(2e-Oxygen Reduction Reaction,2e-ORR)方法实现过氧化氢(h_(2)O_(2))的分散式制备,被认为是具有广阔发展前景的技术之一。为实现高的氧还原活性和理想的h_(2)O_(2)选择性,设计高性能2e-ORR电催化剂是该技术的... 详细信息

利用电化学二电子氧还原(2e-Oxygen Reduction Reaction,2e-ORR)方法实现过氧化氢(h_(2)O_(2))的分散式制备,被认为是具有广阔发展前景的技术之一。为实现高的氧还原活性和理想的h_(2)O_(2)选择性,设计高性能2e-ORR电催化剂是该技术的核心关键。尽管现阶段发展的2e-ORR催化剂已经取得了显著进展,但距离规模化应用仍存在很大的差距。基于此,本文综述了电化学二电子氧还原制备h_(2)O_(2)的最新研究进展,首先简要介绍了反应机理和评价方法,并根据机理分析了影响电催化性能的关键因素。随后围绕贵金属催化剂、碳基催化剂、单原子及分子催化剂的最新研究进展,阐述了提升催化剂活性与h_(2)O_(2)选择性的主要策略。最后,针对电化学二电子氧还原制备h_(2)O_(2)体系面临的关键问题和挑战,对该领域未来的发展趋势进行了展望。

关键词：电化学二电子氧还原反应过氧化氢催化剂选择性

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储能式低温空分系统热力学与经济性分析

化工学报 2024年第7期75卷 2409-2421页

作者：秦晓巧谭宏博温娜西安交通大学制冷与低温工程系陕西西安710049

低温空气分离设备是大型化工系统的高耗能环节,若与液态空气储能技术相结合,可有效平衡电网峰谷负荷,显著提高系统的经济效益。提出了一种储能式低温空分系统(ASU-ESG),利用谷电制取液态空气并储存,在峰电期内液态空气膨胀发电并参与低... 详细信息

低温空气分离设备是大型化工系统的高耗能环节,若与液态空气储能技术相结合,可有效平衡电网峰谷负荷,显著提高系统的经济效益。提出了一种储能式低温空分系统(ASU-ESG),利用谷电制取液态空气并储存,在峰电期内液态空气膨胀发电并参与低温精馏。此方案可有效降低系统在用电峰时的压缩负荷和电力成本,改善空分装置的负荷调节能力。研究结果表明,空气处理量为60000 m^(3)/h的ASU-ESG系统的日均制氧压缩功比功耗为0.378 kW·h/m^(3),储能和释能过程氧提取率分别为89.46%和93.71%。相比常规ASU系统,该系统的谷时压缩负荷提高了28%,峰时压缩负荷降低了20%,可节约年度用电成本5.72%~6.88%,动态回收期仅为3.3~4.3年,全生命周期净现值可达(19714.6~28074.7)万元。本系统可平衡电网峰谷波动,并利用电价差显著提高系统的经济效益。

关键词：空气分离液态空气储能动态回收期