检索结果-南通市图书馆

Selection of aptamers to a non-purified target of native conformation by liquid-SELEX

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Selection of aptamers to a non-purified target of native con...

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： shaohua Li Hui Li Hongmei Ding Fang Wang ningsheng shao Institute of Basic Medical Sciences Academy of Military Medical Science

Background:Aptamers are short single stranded DNA or RNA molecules,which bind specifically to their targets based on their specific tertiary *** to the properties of precise discrimination,easy synthesis and modification, less immunogenicity and small size,aptames are emerging candidates for medical research and *** are evolved from an in vitro selection process known as SELEX(Systematic Evolution of Ligands by Exponential Enrichment) that involves the progressive selection of highly specific ligands by repeated rounds of selection and amplification from a large random synthetic nucleic acid *** traditional SELEX requires a purified

关键词： liquid-SELEX aptamer EMSA catalytic region of glucosyltransferase

Hfq is a global regulator to control the pathogenicity of staphylococcus aureus

在线全文

cnki会议

学校读者我要写书评

暂无评论

Hfq is a global regulator to control the pathogenicity of st...

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Yu Liu Na Wu Jie Dong Yaping Gao Xin Zhang Chunhua Mu ningsheng shao Guang Yang Beijing Institute of Basic Medical Sciences

The Hfq protein is reported to be an RNA chaperone,which is involved in stress response and virulence of several *** ***,Hfq can mediate the interaction between some sRNAs and their target *** it is controversial whether Hfq plays an important role in S. *** this study,it is found that the deletion of hfq gene in *** 8325-4 can induce the surface carotenoid pigments *** hfq mutant is more resistant to the oxidative stress,but the pathogenicity of the mutant is *** reveal that the Hfq protein can be detected

关键词：

The production of extracellular proteins is regulated by RibonucleaseⅢvia two different pathways in Staphylococcus aureus

在线全文

cnki会议

学校读者我要写书评

暂无评论

The production of extracellular proteins is regulated by Rib...

第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Yu Liu Jie Dong Na Wu Yaping Gao Xin Zhang Chunhua Mu ningsheng shao Ming Fan Guang Yang Beijing Institute of Basic Medical Sciences

Objects:Staphylococcus aureus ribonucleaseⅢbelongs to the enzyme family known to degrade double-stranded *** has previously been reported that RNaseⅢcan not influence cell growth but regulate virulence genes express...

关键词：

Non-speicific enhancement effect of siRNA by single stranded polyA in vivo

在线全文

cnki会议

学校读者我要写书评

暂无评论

Non-speicific enhancement effect of siRNA by single stranded...

第四届全国RNA进展研讨会

作者： Li Jie, Yang Guang, Li shaohua, Zhao Qiang, Ding Hongmei, Shen Beifen ,shao ningsheng Beijing Institute of Basic Medical Sciences, Beijing

RNA interference (RNAi)has been a powerful tool in genetic research, but there are many factors influencing siRNAs’s silencing effect including target site accessibility. Here, we found the siRNA-polyA molecules can introduce non-specfic silencing effect to exogenous gene when we used EGFP and RFP as reporter. At the same time, our experiment showed the non-specific effect strengthened alone with the growth of the polyA tail. But more interestingly, when we used ss-polyA as a control, we surprisingly found that ss-polyA with its length to 60nt or more longer could

关键词：

Enrichment and sequence analysis of dimers of Φ29 pRNA mutants

在线全文

cnki会议

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2002年第8期 25-28页

作者： shao ningsheng, YANG Guang, WANG Xin, DING Hongmei, ZHANG Dajin, LI Jie, LIAO Shiqi and FAN Ming(Beijing Institute of Basic Medical Sciences, Beijing 100850, China) Beijing Institute of Basic Medical Sciences Beijing 100850 China

Hexamer of packaging RNA (pRNA) is absolutely required in the packaging of bacteriophage $29 genomic DNA into their procapsid. More and more evidence confirmed that pRNA dimer may be the building block of pRNA hexamer. To better understand sequence required for the dimer formation of pRNA, the pRNA mutants that could easily form stable dimer in vitro were selected by systematic evolution of ligands by exponential enrichment (SELEX). With help of the RNA structure 3.5 software, we found that the pRNA mutants which could form stable dimer had the "Y"-like secondary structures, while monomer pRNA mutants had the "I"-like secondary structures. Our results suggested that except for the "upper-to-lower" or "head-to-head" intermolecular interaction of wide type pRNAs, the dimer of pRNA could also possibly be formed by "head-to-lower" interaction mechanisms.

关键词： pRNA mutant dimer monomer SELEX.

MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

MiR-138 downregulates miRNA processing in HeLa cells by targ...

生命的分子机器及其调控网络——2012年全国生物化学与...

作者： Jie Li Ying Chen Wei Xia Xueting Su shaohua Li Hongmei Ding Wei Wang Hui Li Qiang Zhao ningsheng shao Beijing Institute of Basic Medical Sciences

MicroRNAs(miRNAs) are a class of non-coding small RNAs that consist of approximately 22 nucleotides and are involved in several biological processes by regulating target gene ***-138 has many biological functions and is often downregulated in *** report that the overexpression of miR-138 downregulates target RMND5A(required for meiotic nuclear division 5

关键词：

人巨细胞病毒M抗原表位保守氨基酸突变的分析

在线全文

cnki会议

学校读者我要写书评

暂无评论

生物工程学报 2008年第7期24卷 1128-1132页

作者：王本旭刘展刘玉沈倍奋柳川邵宁生北京军区疾病预防控制中心北京100042 北京市怀柔区妇幼保健医院北京101400 军事医学科学院基础医学研究所北京100850

为确定人巨细胞病毒M抗原表位MAD的关键氨基酸残基,以MAD多肽序列为基础,分别将保守氨基酸残基单一突变为甘氨酸残基,构建各自突变体,然后与人源Fc的N端融合,通过原核表达载体pET32-Fc表达融合蛋白MAD-Fc,经proteinA柱亲和纯化得到各突... 详细信息

为确定人巨细胞病毒M抗原表位MAD的关键氨基酸残基,以MAD多肽序列为基础,分别将保守氨基酸残基单一突变为甘氨酸残基,构建各自突变体,然后与人源Fc的N端融合,通过原核表达载体pET32-Fc表达融合蛋白MAD-Fc,经proteinA柱亲和纯化得到各突变体纯品。通过ELISA及Western blotting方法验证各突变体特异结合羊抗HCMV多抗间的差异,从而确定表位关键氨基酸残基。结果显示,将MAD中的谷氨酰胺残基单突变为甘氨酸残基后,MADQ-G结合羊抗HCMV多抗活性大大降低,差异显著;而其他氨基酸残基单突变时,对MAD活性影响程度很小。由此得出结论:MAD结合羊抗HCMV多抗的活性与谷氨酰胺残基有关。

关键词：人巨细胞病毒抗原表位突变

The protective antibodies induced by a novel epitope of human tumor necrosis factor alpha could suppress the development of of collagen-induced arthritis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The protective antibodies induced by a novel epitope of huma...

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Jie Dong Yaping Gao Yu Liu Jinxia Shi Jiannan Feng Zhanguo Li Heping Pan Yanning Xue Chuan Liu Beifen Shen ningsheng shao Guang Yang Beijing Institute of Basic Medical Sciences People's HospitalPeking University Department of Rheumatology and Immunology People's HospitalPeking University

Tumor necrosis factor alpha(TNF-) is a major inflammatory mediator that exhibits actions directed towards both tissue destruction and recovery from ***,two antibodies against human TNF-(hTNF-) are available, which are widely used for the clinic treatment of certain inflammatory *** work was undertaken to identify a novel functional epitope of hTNF-.We performed screening peptide library against anti-hTNFantibodies,ELISA and competitive ELISA to obtain the epitope of hTNF-.The key residues of the epitope were identified by means of combinatorial alanine scanning and sitespecific mutagenesis. The N terminus(4-15 aa) of hTNF- was proved as a novel

关键词：

在线全文

cnki会议

学校读者我要写书评

暂无评论

Argonaute蛋白的结构与功能

医学分子生物学杂志 2014年第1期11卷 37-42页

作者：苏雪婷李洁邵宁生军事医学科学院基础医学研究所北京市100850

Argonaute蛋白是RNA介导的转录后基因调控的关键蛋白。它为非编码小RNA提供锚位点，达到降解靶基因或者抑制翻译的目的。Argonaute蛋白广泛存在于真核生物中，在进化上高度保守。有关Argonaute蛋白的最新研究进展主要集中在人源AGO蛋白... 详细信息

Argonaute蛋白是RNA介导的转录后基因调控的关键蛋白。它为非编码小RNA提供锚位点，达到降解靶基因或者抑制翻译的目的。Argonaute蛋白广泛存在于真核生物中，在进化上高度保守。有关Argonaute蛋白的最新研究进展主要集中在人源AGO蛋白的晶体结构解析以及在此基础上对于非编码小RNA转录后调控机制的进一步理解；翻译后修饰对Argonaute蛋白功能的调控及Argonaute蛋白的新功能，譬如Argonaute蛋白在基因转录和DNA损伤修复过程中所发挥的作用。文章主要就哺乳动物Argonaute蛋白的结构与功能研究最新进展做一综述。

关键词： Argonaute蛋白基因沉默转录后调控翻译后修饰