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First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

World Journal of Gastroenterology 2023年第41期29卷 5683-5698页

作者： ning-Zu Jiang ming-Zhen Bai Chong-Fei Huang Ze-Long Ma Ru-Yang Zhong Wen-Kang Fu Long Gao Liang Tian ning-ning mi Hai-Dong Ma Ya-Wen Lu Zi-Ang Zhang Jin-Yu Zhao Hai-Ying Yu Bao-Ping Zhang Xian-Zhuo Zhang Yan-Xian Ren Chao Zhang Yong Zhang Ping Yue Yan-Yan Lin Wen-Bo Meng The First Clinical Medical College Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General Surgery The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General Surgery The First Hospital of Lanzhou University and Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu ProvinceLanzhou 730000Gansu ProvinceChina

BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were posi

关键词： Carcinosarcoma Drug resistance Xenograft Cell line Establishment

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Cytokines predict virological response in chronic hepatitis B patients receiving peginterferon alfa-2a therapy

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World Journal of Clinical Cases 2020年第11期8卷 2255-2265页

作者： Wen-Kang Fu Jie Cao ning-ning mi Chong-Fei Huang Long Gao Jin-Duo Zhang Ping Yue Bing Bai Yan-Yan Lin Wen-Bo Meng The First Clinical Medical School Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Laboratory Department of the First Hospital of Lanzhou University Lanzhou 730000Gansu ProvinceChina Special minimally Invasive Surgery Department The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Institute of Genetics School of Basic Medical SciencesLanzhou UniversityLanzhou 730000Gansu ProvinceChina Institute of Hepatopancreatobiliary of Gansu Province Lanzhou 730000Gansu ProvinceChinao Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province Lanzhou 730000Gansu ProvinceChina

BACKGROUND Chronic hepatitis B virus infection remains a major global public health problem.Peginterferon-alpha-2a(PEG-IFN)has direct antiviral and immunoregulatory effects,and it has become one of the first choice drugs for the treatment of chronic hepatitis B(CHB).Cytokines play an important role in immunity,and they directly inhibit viral replication and indirectly determine the predominant pattern of the host immune response.AIM To determine the correlation between cytokine/chemokine expression levels and response to PEG-IFN treatment in patients with CHB.METHODS Forty-six kinds of cytokines were analyzed before PEG-IFN therapy and at 24 wk during therapy in 26 CHB patients.RESULTS The monokine induced by INF-γ(CXCL9)and serum interferon-inducible protein 10(IP-10)levels at baseline were higher in virological responders than in nonvirological responders(NRs)and decreased during treatment,whereas the NRs did not exhibit significant changes.The macrophage inflammatory protein 1d(miP-1d)levels at baseline and during treatment were significantly higher in the virological responders than in the NRs,while thymus and activation-regulated chemokine(TARC)levels at baseline and during treatment were significantly lower in the virological responders than in the NRs.The CXCL9,IP-10,miP-1d,and TARC baseline levels exhibited the expected effects for interferon treatment.The area under the receiver operating characteristic curve values of CXCL9,IP-10,miP-1d,and TARC for predicting virological responses were 0.787,0.799,0.787,and 0.77(P=0.01,0.013,0.01,and 0.021),respectively.CONCLUSION We found that cytokine levels before and during treatment may represent potential biomarkers to select CHB patients who can respond to PEG-IFN.Therefore,cytokines can be used as an indicator of antiviral drug selection before CHB treatment.

关键词： Chronic hepatitis B Peginterferon-alpha-2a Cytokine/chemokine CXCL9 Interferon-inducible protein 10 Thymus and activation-regulated chemokine

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Associations between serum uric acid and hepatobiliary-pancreatic cancer:A cohort study

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World Journal of Gastroenterology 2020年第44期26卷 7061-7075页

作者： Chong-Fei Huang Jun-Jun Huang ning-ning mi Yan-Yan Lin Qiang-Sheng He Ya-Wen Lu Ping Yue Bing Bai Jin-Duo Zhang Chao Zhang Teng Cai Wen-Kang Fu Long Gao Xun Li Jin-Qiu Yuan Wen-Bo Meng The First Clinical Medical School Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Special minimally Invasive Surgery Department The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Institute of Genetics School of Basic Medical SciencesLanzhou UniversityLanzhou 730000Gansu ProvinceChina Gansu Province Institute of Hepatopancreatobiliary Lanzhou 730000Gansu ProvinceChina Gansu Province Key Laboratory Biotherapy and Regenerative Medicine Lanzhou 730000Gansu ProvinceChina Scientific Research and Planning Department The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Clinical Research Center and Scientific Research Center The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107Guangdong ProvinceChina The Fifth Department of General Surgery The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina

BACKGROUND Uric acid is the end product of purine metabolism.Previous studies have found that serum uric acid(SUA)levels are associated with the total cancer risk.However,due to the dual effect of uric acid on cancer,the relationship between the SUA levels and most specific-site cancer remains unclear.AIM To investigate the associations between the SUA levels and incidence of hepatobiliary-pancreatic cancer.METHODS In this prospective cohort study,444462 participants free of cancer from the UK Biobank were included.The SUA levels were measured at baseline,and the incidence of hepatobiliary-pancreatic cancer was determined by contacting the cancer registry.The hazard ratios(HRs)and 95%confidence intervals(CIs)between the SUA levels and hepatobiliary-pancreatic cancer were investigated using multiple adjusted Cox regression models adjusted for potential confounders.RESULTS In total,920 participants developed liver,gallbladder,biliary tract or pancreatic cancer during a median of 6.6 yrs of follow-up.We found that the HR of pancreatic cancer in the highest SUA group was 1.77(95%CI:1.29-2.42)compared with that in the lowest group.After stratifying by gender,we further found that SUA was associated with an increased risk of pancreatic cancer only among the females(highest quartile vs lowest quartile HR 2.04,95%CI:1.35-3.08).Among the males,the SUA levels were positively associated with the gallbladder cancer risk(highest quartile vs lowest quartile HR 3.09,95%CI:1.28-7.46),but a U-shaped association with the liver cancer risk was observed(P-nonlinear=0.03).CONCLUSION SUA is likely to have gender-specific effects on hepatobiliary-pancreatic cancer.High SUA levels are a risk factor for pancreatic cancer in females and gallbladder cancer in males.A U-shaped association with the liver cancer risk was identified.

关键词： Uric acid Liver neoplasms Pancreatic neoplasms Gallbladder neoplasms Biliary tract neoplasms Cohort studies

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Unraveling the evolution of oxygen vacancies in TiO/Cu and its role in COhydrogenation

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Science China Chemistry 2024年

作者： Ke Wang Fanxing Zhang ning Cao Ying Bao mi Yan Keping Yan Pengfei Xie State Key Laboratory of Baiyunobo Rare Earth Resource Researches and Comprehensive Utilization Baotou Research Institute of Rare Earths School of Materials Science and Engineering Zhejiang University Institute of Zhejiang University-Quzhou College of Chemical and Biological Engineering Zhejiang University Department of Chemistry Western Washington University Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering

The remarkable contribution of oxygen vacancies has been revealed by the operando spectroscopies for methanol synthesis from CO2hydrogeneration on the reducible metal oxide-supported copper catalysts. However, a challenge remains in the intrinsic understanding of the evolution and advantage of oxygen vacancies for methanol synthesis. Here we prepare the TiO2–x/Cu with different oxygen vacancy densities by adjusting the ball-milling frequency. At the optimal condition, a TiO2–x/Cu with more oxygen vacancies delivers an excellent methanol yield of 26.5 mmol g-1h-1at 300 °C with a selectivity of more than 70%. The combined analysis of experimental characterizations and theoretical calculations reveals that the mutual dispersions of TiO2–xand Cu driven by mechanical energy induce the electron rearrangement in the d orbital of the Ti atom and relax the Ti–O binding at the interface, which facilitates the formation of oxygen vacancies that further reduce the barrier of CO2hydrogenation to*HCOO due to higher nucleophilicity of titanium ions.

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High-resolution remote thermometry based on Bi3+-doped 0D zinc halide hybrids

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Science China Chemistry 2024年第8期 2599-2605页

作者： Cui-mi Shi Shu-Hua Xue Jin-Yun Wang Liang-Jin Xu Zhong-ning Chen State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China University of Chinese Academy of Sciences

Developing highly sensitive optical thermometers is of great significance due to their capability to enable remote and non-contact temperature measurements, rendering them highly applicable in diverse and harsh environments. Herein we report a temperature-dependent phosphor of 0D metal halide hybrid by incorporating Bi3+into the（MePPh3）2ZnCl4matrix. Through Bi3+doping,the initially non-luminescent（MePPh3）2ZnCl4matrix exhibits a deep-blue emission centered at 453 nm, with a photoluminescence quantum yield（PLQY） of 5.71% and a Stokes shift of 75 nm at room temperature. Experimental characterization demonstrates that exciton-like luminescence of Bi3+is mainly responsible for the blue emission. Single crystals of Bi3+-doped（MePPh3）2ZnCl4show an unusual correlation between photoluminescence（PL） lifetime and temperature. Particularly, the dependence of luminescence lifetime on temperature is most remarkable in the temperature range of 80 to 100 K with an exceptional sensitivity up to 0.09 K-1, representing one of the best levels for thermometry based on PL decay lifetime. Our work not only provides a viable strategy for designing a novel, environmentally friendly, and stable blue emitter, but also paves the way for precise thermometric application at low temperature.

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High-resolution remote thermometry based on Bi-doped 0D zinc halide hybrids

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Science China Chemistry 2024年

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Reynosin protects neuronal cells from microglial neuroinflammation by suppressing NLRP3 inflammasome activation mediated by NADPH oxidase

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Chinese Journal of Natural Medicines 2024年第6期22卷 486-500页

作者： YANG Yanqiu CHE Yue FANG mingxia YAO Xiaohu ZHOU Di WANG Feng CHEN Gang LIANG Dong LI ning HOU Yue Key Laboratory of Bioresource Research and Development of Liaoning Province College of Life and Health SciencesNorth-eastern UniversityShenyang 110000China National Frontiers Science Center for Industrial Intelligence and Systems Optimization Key Laboratory of Data Analytics and Optimization for Smart IndustryMinistry of EducationNortheastern UniversityShenyang 110000China School of Traditional Chinese Materia Medica Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning provinceKey Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang CityShenyang Pharmaceutical UniversityShenyang 110000China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources School of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGuilin 541000China

Neuroinflammation,mediated by the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing-3(NLRP3)inflammasome,is a significant contributor to the pathogenesis of neurodegenerative diseases(NDDs).Reynos-in,a natural sesquiterpene lactone(SL),exhibits a broad spectrum of pharmacological effects,suggesting its potential therapeutic value.However,the effects and mechanism of reynosin on neuroinflammation remain elusive.The current study explores the effects and mechanisms of reynosin on neuroinflammation using mice and BV-2 microglial cells treated with lipopolysaccharide(LPS).Our findings reveal that reynosin effectively reduces microglial inflammation in vitro,as demonstrated by decreased CD11b expression and lowered interleukin-1 beta(IL-1β)and interleukin-18(IL-18)mRNA and protein levels.Correspondingly,in vivo,results showed a re-duction in the number of Iba-1 positive cells and alleviation of morphological alterations,alongside decreased expressions of IL-1βand IL-18.Further analysis indicates that reynosin inhibits NLRP3 inflammasome activation,evidenced by reduced transcription of NLRP3 and caspase-1,diminished NLRP3 protein expression,inhibited apoptosis-associated speck-like protein containing a CARD(ASC)oli-gomerization,and decreased caspase-1 self-cleavage.Additionally,reynosin curtailed the activation of nicotinamide adenine dinuc-leotide phosphate(NADPH)oxidase,demonstrated by reduced NADP^(+)and NADPH levels,downregulation of gp91^(phox) mRNA,pro-tein expression,suppression of p47^(phox) expression and translocation to the membrane.Moreover,reynosin exhibited a neuroprotective effect against microglial inflammation in vivo and in vitro.These collective findings underscore reynosin’s capacity to mitigate mi-croglial inflammation by inhibiting the NLRP3 inflammasome,thus highlighting its potential as a therapeutic agent for managing neuroinflammation.

关键词： microglia NLRP3 inflammasome Reactive oxygen species NADPH oxidase Neuron Reynosin

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红医文化融入新时代医学生医德教育的实践路径研究

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中国医学伦理学 2024年第6期37卷 651-656页

作者：魏咪刘宁西安医学院陕西西安710021 陕西师范大学马克思主义学院陕西西安710064

红医文化是中国先进革命文化的重要组成部分,诞生于人民卫生事业伟大实践中,是新时代医学生医德教育可借鉴利用的重要资源。当前医学生价值观受到多重影响,而医德教育也存在边缘化、无系统化等问题。红医文化丰富了社会主义医德建设内涵... 详细信息

红医文化是中国先进革命文化的重要组成部分,诞生于人民卫生事业伟大实践中,是新时代医学生医德教育可借鉴利用的重要资源。当前医学生价值观受到多重影响,而医德教育也存在边缘化、无系统化等问题。红医文化丰富了社会主义医德建设内涵,是医德教育的重要抓手,融入医德教育将有助于时代新人培育,加强医学人文教育。可以从以下几方面着手将红医文化融入新时代医学生医德教育:发挥主阵地主渠道作用,优化校园文化环境,用好实践载体的功能,强化思想引领与形势教育,搭建好网络叙事平台。

关键词：红医文化新时代医学生医德

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Multi-wavelength nanowire micro-LEDs for future high speed optical communication

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Opto-Electronic Advances 2024年第3期7卷 1-3页

作者： Ayush Pandey Zetian mi Department of Electrical Engineering and Computer Science University of MichiganAnn ArborMI 48109USA

The future of optoelectronics is directed towards small-area light sources,foremost being microLEDs.However,their use has been inhibited so far primarily due to fabrication and integration challenges,which impair efficiency and yield.Recently,bottom-up nanostructures grown using selective area epitaxy have garnered attention as a solution to the aforementioned issues.Prof.Lan Fu et.***.have used this technique to demonstrate uniform p-i-n core-shell InGaAs/InP nanowire array light emitting diodes.The devices are capable of voltage and geometry-controlled multi-wavelength and high-speed operations.Their publication accentuates the wide capabilities of bottom-up nanostructures to resolve the difficulties of nanoscale optoelectronics.

关键词： communication high operations

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Topological Dirac-vortex microcavity laser for robust on-chip optoelectronics

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Light(Science & Applications) 2024年第4期13卷 591-592页

作者： Yuanpeng Wu Zetian mi Department of Electrical Engineering and Computer Science University of Michigan1301 Beal AvenueAnn ArborMI 48109USA

Dirac-vortex microcavity laser based on InAs/InGaAs quantum dots have been experimentally realized on silicon substrate.The topological laser features a large spectral range and high robustness against variations such... 详细信息

关键词： laser optoelectronics cavity

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