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番茄自然群体磷利用效率分析及耐低磷种质筛选

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园艺学报 2024年第5期51卷 993-1004页

作者：李品甘宁陈家伟项思翔沈静漪欧阳波卢永恩华中农业大学园艺林学学院果蔬园艺作物种质创新与利用全国重点实验室武汉430070

以蛭石为基质,通过营养液控制,在幼苗期对包括196份材料的番茄自然群体进行了正常磷浓度及其10%和1%(830、83和8.3μmol·L^(-1)HPO_(4)^(2-))磷水平处理,并以幼苗地上部干、鲜质量、株高和叶绿素含量等指标为依据,对该自然群体进行了... 详细信息

以蛭石为基质,通过营养液控制,在幼苗期对包括196份材料的番茄自然群体进行了正常磷浓度及其10%和1%(830、83和8.3μmol·L^(-1)HPO_(4)^(2-))磷水平处理,并以幼苗地上部干、鲜质量、株高和叶绿素含量等指标为依据,对该自然群体进行了磷效率评估和耐低磷材料筛选。研究结果表明,地上部鲜质量和干质量在所检测指标中变异较大,材料间存在丰富的遗传变异。以低磷处理地上部鲜质量、干质量与正常磷处理的比值作为耐低磷评价指标,通过聚类分析,分别获得了1%磷水平下3个耐低磷番茄材料TS-653、yf4和pl1,和10%磷水平下3个耐低磷番茄材料TS-26、TS-643和TS-653,并分析了它们的相对磷含量以及地上部和地下部的磷含量比值。利用模糊隶属函数法计算各材料的磷效率综合值,基于散点图,筛选出在3个磷水平下均表现为高效的番茄种质TS-614。

关键词：番茄种质资源耐低磷磷高效磷效率综合值

Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification

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Journal of Pharmaceutical Analysis 2022年第6期12卷 913-922页

作者： Liu Liu Juncheng Hong Wenhai Wang Shu Xiao Hongzhen Xie Qiqin Wang ning gan School of Material Science and Chemical Engineering Ningbo UniversityNingboZhejiang315211China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China

In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen *** this system,bar 1 was labeled with the *** ***,and then bar 2 was functionalized with *** *** *** introduced,pathogen-Apt complexes would form and be released into the supernatant from bar *** agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on ***,the encoded probes would be detached from bar 2 to generate FL signals in the ***,the pathogens can resume their free state and initiate next *** swim between the two bars,and the FL signals can be gradually enhanced to maximum after several *** FL signals from released encoded probes can be used to detect the *** particular,live pathogens can be distinguished from dead ones by using an *** detection limits and linear range for *** *** 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,***,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.

关键词： Aptasensor Live bacteria Multiple detection Multicolor perovskite quantum dots Dual-stirring-bar

The Structure of Vector Bundles on Non-primary Hopf Manifolds

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Chinese Annals of Mathematics,Series B 2020年第6期41卷 929-938页

作者： ning gan Xiangyu ZHOU School of Sciences Jimei Uni vers计yXiamen 361021FujianChina Institute of Mathematics Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China

Let X be a Hopf manifold with non-Abelian fundamental group and E be a holomorphic vector bundle over X,with trivial pull-back to C^n-{0}.The authors show that there exists a line bundle L over X such that E■L has a nowhere vanishing *** is proved that in case dim(X)≥3,π*(E)is trivial if and only if E is filtrable by vector *** the structure theorem,the authors get the cohomology dimension of holomorphic bundle E over X with trivial pull-back and the vanishing of Chern class of E.

关键词： Hopf manifolds Holomorphic vector bundles Exact sequence Cohomology Filtration Chern class

The Cohomology of Line Bundles on Non-primary Hopf Manifolds

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Acta Mathematica Sinica,English Series 2007年第2期23卷 289-296页

作者： ning gan Xiang Yu ZHOU Institute of Mathematics Academy of Mathematics and Systems ScienceChinese Academy of Sciences

The purpose of the present paper is to give an elementary method for the computation of the cohomology groups Hq（X,Ω^p X（L））, （0 ≤q ≤ n） of an n-dimensional non-primary Hopf manifold X with arbitrary fundamental group. We use the method of Zhou to generalize the results for primary Hopf manifolds and non-primary Hopf manifold with an Abelian fundamental group.

关键词： non-primary Hopf manifold cohomology line bundles Douady sequence

化瘀解痉方联合尼莫地平治疗动脉瘤性蛛网膜下腔出血后脑血管痉挛患者的疗效及对其血管内皮功能的影响

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世界中西医结合杂志 2024年第7期19卷 1398-1402,1408页

作者：齐雯婧甘宁臧伟胡博李佳单玉超徐智杰赵陈祎田楠保定市第一中心医院体检科河北保定071000 保定市第一中心医院神经外三科河北保定071000 保定市第一中心医院医务处河北保定071000

目的探讨化瘀解痉方联合尼莫地平治疗动脉瘤性蛛网膜下腔出血(Subarachnoid hemorrhage,SAH)后脑血管痉挛患者的疗效及对其血管内皮功能的影响。方法选取2022年1月-2023年1月期间保定市第一中心医院收治的SAH后脑血管痉挛患者90例,按随... 详细信息

目的探讨化瘀解痉方联合尼莫地平治疗动脉瘤性蛛网膜下腔出血(Subarachnoid hemorrhage,SAH)后脑血管痉挛患者的疗效及对其血管内皮功能的影响。方法选取2022年1月-2023年1月期间保定市第一中心医院收治的SAH后脑血管痉挛患者90例,按随机数字表法分为对照组和观察组,每组各45例。对照组给予尼莫地平治疗,观察组在对照组基础上联合化瘀解痉方治疗,均连续治疗15 d。观察两组患者治疗前后神经功能缺损评分(National institute of health stroke scale,NIHSS)、Hunt-Hess分级评分、意识障碍程度评分(Glasgow coma scale,GCS)、血管内皮功能指标[内皮素-1(Endothelin-1,ET-1)、血管内皮生长因子(Vascular endothelial growth factor,VEGF)]、脑血流指标[大脑中动脉平均流速(Mean flow velocity of middle cerebral artery,MCA-Vm)、颈内动脉颅外段平均流速(Mean extracranial velocity of internal carotid artery,VICA-Vm)、脑血管痉挛(Cerebral vasospasm,CAS)指数]改善情况,临床总有效率及不良反应情况。结果治疗后两组患者NIHSS、Hunt-Hess评分均较治疗前降低,GCS评分均较治疗前升高,差异有统计学意义(P<0.05);且观察组NIHSS、Hunt-Hess评分均明显低于对照组,GCS评分明显高于对照组,差异有统计学意义(P<0.05)。治疗后两组患者ET-1、VEGF水平均较治疗前降低,差异有统计学意义(P<0.05);且观察组ET-1、VEGF水平均明显低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者MCA-Vm、VICA-Vm及CAS指数均较治疗前降低,差异有统计学意义(P<0.05);且观察组MCA-Vm、VICA-Vm及CAS指数均明显低于对照组,差异有统计学意义(P<0.05)。治疗后观察组临床总有效率91.11%(41/45)明显高于对照组73.33%(33/45),差异有统计学意义(P<0.05)。治疗期间,两组患者均未发生不良反应,也未出现死亡病例。结论化瘀解痉方联合尼莫地平能够改善SAH后脑血管痉挛患者的血管内皮功能,增加脑血流灌注,减轻神经功能损伤,控制病情发展,疗效显著,安全性高。

关键词：脑血管痉挛动脉瘤蛛网膜下腔出血化瘀解痉方尼莫地平神经功能缺损脑血流指标血管内皮功能

Amperometric detection of gold by differential pulse voltammetry using a DNA biosensor

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Rare Metals 2007年第2期26卷 169-175页

作者： gan ning WANG Zhiying XU Weiming PAN Jianguo The State Key Laboratory Base of Novel Functional Materials and Preparation Science Ninbo University Ningbo 315211 China

A DNA biosensor with [Ru（DA-bpy）3]Cl2（DA-bpy：4,4＇-diamino-2,2＇-bipyridine）（RuL） as the electrochemical probe was prepared on pyrolytic graphite electrode （PGE） through the supramolecular interaction between RuL complex and DNA template. Cyclic voltammetry of RuL-DNA film showed a pair of stable and reversible peaks corresponding to the Ru（Ⅲ）/Ru（Ⅲ） redox potential of-0.165 V versus AglAgCl in pa 7.4 0.1 mol·L^-1Tris-HCl. The electron transfer was expected across the double-strand DNA by an ＂electron tunneling＂ mechanism. When the DNA biosensor was immerged in gold （Ⅲ） buffer solution, the current peak signal （I） of the RuL-DNA supramolecular depressed and △I was linear in the concentration range of Au ion from 1×10^-7 to 2×10^-5 mol·L^-1 with a regression coefficient of 0.9879. The detection limit was 5×10^-8 mol·L^-1. The developed procedures were applied to the analysis of synthetic samples of real materials with good sensitivity and selectivity.

关键词： gold(Ⅲ) determination differential pulse voltammetry (DPV) DNA biosensor

酸性媒蓝紫-微分吸附计时电位法测定合金钢中微量钙

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冶金分析 2006年第2期26卷 5-8页

作者：干宁宁波大学理学院化学系浙江宁波315211 南京大学化学系江苏南京210009

采用4-羟基-3-(2-羟基萘偶氮)苯磺酸钠(酸性媒蓝紫,缩写为SVRS)-微分吸附计时电位法(DACOP)测定合金钢中微量钙。在0.2 mol/L NH4Cl-NH3.H2O-8×10-4mol/LSVRS(pH 8.0)底液中,Ca-SVRS络合物在-0.9 V电位处产生灵敏还原峰,峰高的增量与... 详细信息

采用4-羟基-3-(2-羟基萘偶氮)苯磺酸钠(酸性媒蓝紫,缩写为SVRS)-微分吸附计时电位法(DACOP)测定合金钢中微量钙。在0.2 mol/L NH4Cl-NH3.H2O-8×10-4mol/LSVRS(pH 8.0)底液中,Ca-SVRS络合物在-0.9 V电位处产生灵敏还原峰,峰高的增量与钙浓度成正比。用于钙的定量分析,线性范围为1×10-7~1.5×10-5mol/L,相对标准偏差为1.9%(n=10),检出限为5.0×10-8mol/L。对实际合金钢样品中钙进行分析,并与ICP-AES法进行比较,结果吻合。

关键词：酸性媒蓝紫钙合金钢微分吸附计时电位法

Intermittent fasting boosts antitumor immunity by restricting CD11b^(+)Ly6C^(low)Ly6G^(low) cell viability through glucose metabolism in murine breast tumor model

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Food Science and Human Wellness 2024年第4期13卷 2327-2345页

作者： Chenghao Fu Zhehao Liang Zemiao Niu ning Chen Yuemin Li Zhenhua Liang Yanwei Huo Hao Xi Rong Wang Yonghuan Yan Xiaoruo gan Mengtian Wang Yun Huang Yan Zhang Mingming Gao Pin Lü Postdoctoral Station for Basic Medicine Cardiovascular Medical Science CenterDepartment of Cell BiologyKey Laboratory of Neural and Vascular Biology of Ministry of EducationHebei Medical UniversityShijiazhuang 050017China Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050091China Laboratory of Lipid Metabolism Department of Biochemistry and Molecular BiologyKey Laboratory of Medical Biotechnology of Hebei ProvinceShijiazhuang 050017China

Intermittent fasting can benefit breast cancer patients undergoing chemotherapy or ***,it is still uncertain how to select immunotherapy drugs to combine with intermittent *** we observed that two cycles of fasting treatment significantly inhibited breast tumor growth and lung tissue metastasis,as well as prolonged overall survival in mice bearing 4T1 and 4T07 breast *** this process,both the immunosuppressive monocytic-(M-)and granulocytic-(G-)myeloid-derived suppressor cell(MDSC)decreased,accompanied by an increase in interleukin(IL)7R^(+)and granzyme B^(+)T cells in the tumor ***,we observed that Ly6G^(low)G-MDSC sharply decreased after fasting treatment,and the cell surface markers and protein mass spectrometry data showed potential therapeutic *** investigation revealed that glucose metabolism restriction suppressed the splenic granulocytemonocyte progenitor and the generation of colony-stimulating factors and IL-6,which both contributed to the accumulation of *** the other hand,glucose metabolism restriction can directly induce the apoptosis of Ly6G^(low)G-MDSC,but not Ly6G^(high)*** summary,these results suggest that glucose metabolism restriction induced by fasting treatment attenuates the immune-suppressive milieu and enhances the activation of CD3^(+)T cells,providing potential solutions for enhancing immune-based cancer interventions.

关键词： Intermittent fasting Ly6G^(low)myeloid-derived suppressor cell apoptosis Extramedullary hematopoiesis Colony stimulating factor Glucose metabolism restriction

Investigation of the Buffering Function of Aluminum being in Equilibrium with the Mineral Kaolinite in Acidic Natural Water

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Chinese Chemical Letters 2000年第7期11卷 655-658页

作者： Feng LIU Shu Ping BI ning gan(Department of Chemistry, State Key Laboratory of Pollution Control & Resource Reuse,Nanjing University, Nanjing 210093) Department of Chemistry State Key Lab. Poll. Contr. Rsrc. R. Nanjing University Nanjing 210093 China

This paper presents a computer model to investigate the buffering function of aluminum being in equilibrium with mineral kaolinite in acidic natural water. Factors affecting the theoretical pH values are discussed. Th... 详细信息

关键词： kaolinite aluminum buffering function