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Science China Chemistry 2025年

作者： Lulu Chen Yichao Huang Jiajun Wang Meihong Liao Zhiyuan Liu Meiqi Liu Hanqing Cai Lin Wang Lidong Gao Dawei Hu Lianming Zhao ning pu Zhuangjun Fan State Key Laboratory of Chemical Safety School of Materials Science and Engineering China University of Petroleum (East China) School of Mechanical and Electronic Engineering Qingdao Binhai University Qingdao Chuangqi New Energy Catalysis Technology Co.Ltd. Sinopec Research Institute of Petroleum Processing Co.Ltd. Institute of Energy Hefei Comprehensive National Science Center

Insight into the growth behavior and electronic regulation of platinum （Pt） on various transition metal supports is paramount in developing high-performing electrocatalysts for hydrogen evolution reactions （HER）.Herein,we studied the influence of molybdenum-based supports （MoX,X=C,N,P,and S） on the growth behavior and electronic regulation of *** found that the formation energy variations between Pt single atom and clusters on MoX supports play a pivotal role in the growth behavior of ***,the electronic regulation of Pt induced by metal-support interaction may reflect the valence changes of Pt in Pt-MoX/*** Pt-MoC/C catalyst with a moderate valence state of Pt exhibits the best HER activity with an overpotential of 12.0 mV at 10 mA cm-2and a mass activity of 27.1 A mgPt-1,12.3 times as high as that of commercial 20 wt% Pt/*** work provides constructive guidance for the design of high-performance HER catalysts.

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Meta- analysis of association between K469E polymorphism of the ICAM-1 gene and retinopathy in type 2 diabetes

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 603-607页

作者： Wen-Ying Fan ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalBeijing Ophthalmology and Visual Sciences Key LaboratoryCapital Medical University

AIM: To collectively evaluate the association of intercellular adhesion molecule-1(ICAM-1) gene K469 E polymorphism(rs5498) with diabetic retinopathy(DR) in patients with type 2 diabetic mellitus(T2DM). METHODS: Overall review of available literatures relating K469 E polymorphism to the risk of DR was conducted on 4 electronic databases. Meta-analysis was performed by Stata 12.0 to calculate pooled odds ratios(ORs). Potential sources of heterogeneity and bias were ***: Seven studies with genotype frequency data including 1120 cases with DR and 956 diabetic controls free of DR were included. Meta-analysis did not show significant association of K469 E polymorphism with DR(P >0.05). A statistically significant association was detected between the K469 E polymorphism and proliferative DR(PDR) in Asians only in dominant model(GG+AG vs AA) with pooled OR of 0.729(95%CI: 0.564-0.942, P=0.016, P heterogeneity=0.143), however, this association was not detected in recessive model(AG +AA vs GG;OR=1.178, 95%CI: 0.898-1.545, P =0.236, P heterogeneity=0.248)or allelic model(G vs A; OR=0.769, 95% CI: 0.576-1.026,P =0.074, P heterogeneity=0.094). No publication bias was found by Funnel plot, Begg’s and Egger’s test. CONCLUSION: This research found no statistically significant association between ICAM-1 gene K469 E polymorphism and DR in patients with T2 DM, but showed significant association of the K469 E polymorphism with PDR in Asian diabetic patients only in dominant model. Further investigation would be required to consolidate the conclusion.

关键词： K469E polymorphism rs5498 intercellular adhesion molecule-1 diabetic retinopathy type 2 diabetes Meta-analysis

Functional characterization of a Δ6 fatty acid desaturase gene and its 5′-upstream region cloned from the arachidonic acid-rich microalga Myrmecia incisa Reisigl(Chlorophyta)

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Journal of Oceanology and Limnology 2018年第6期36卷 2308-2321页

作者： ZHANG Li CAO Haisheng ning pu ZHOU Zhigang Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Conferred by Ministry of Education Shanghai Ocean University National Demonstration Center for the Experimental Teaching of Fisheries Science Shanghai Ocean University International Research Center for Marine Biosciences Conferred by Ministry of Science and Technology Shanghai Ocean University

It is suggested that Δ6 fatty acid desaturase(FAD) plays a critical role in the biosynthesis of polyunsaturated fatty acids in plants and microalgae. But why does it adapt to the changed environments such as nitrogen starvation is seldom understood. One Δ6 FAD gene( MiD6 fad) from an arachidonic acidrich microalga M yrmecia incisa Reisigl(Chlorophyta) was first heterologously expressed in S accharomyces cerevisiae for the identification of function. The fatty acid profile of transgenic yeast detected by gas chromatography-mass spectrometry illustrated that the enzyme MiD6 FAD could convert linoleic and ?-linolenic acids to γ-linolenic and stearidonic acids, respectively, demonstrating that M iD6 fad encoded a Δ6 FAD. A 1 965-bp fragment of the cloned 2 347-bp 5′-upstream region of M iD6 fad was next subcloned and fused upstream with green fluorescent protein(GFP) gene to replace the GAL1 promoter of the vector pYES2. The generated construct was transformed into S. cerevisiae for function determination. Confocal microscopic images of the transformed line illustrated that this inserted fragment could drive GFP expression, which was further verified by fluorescence intensity quantification and Western blot analysis using antiGFP antibody. The conversion efficiency(approximately 2%-3%) of MiD6 FAD was much lower than the reported ? 3 FAD and Δ6 elongase in this microalga, suggesting that MiD6 FAD catalysed the possible ratelimiting step for ArA biosynthesis. The presence of several putative c is-acting regulatory elements in this identified promoter sheds new light on the regulation mechanism research of Δ6 FAD transcription for the ArA production in M. incisa in changing environmental factors.

关键词： arachidonic acid(ArA) fatty acid desaturase(FAD) green fl uorescent protein(GFP) green microalga Saccharomyces cerevisiae 5′-upstream region(5′-USR)

The relationship between insulin resistance/β-cell dysfunction and diabetic retinopathy in Chinese patients with type 2 diabetes mellitus： the Desheng Diabetic Eye Study

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International Journal of Ophthalmology(English edition) 2018年第3期11卷 493-500页

作者： Yun-Yun Li Xiu-Fen Yang Hong Gu Torkel Snellingen Xi-pu Liu ning-pu Liu Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Sciences Key Laboratory Beijing 100730 China Department of Ophthalmology Friendship Hospital Capital Medical University Beijing 100050 China Department of Ophthalmology Lihuili Hospital Ningbo 315040 Zhejiang Province China Sekwa Institute of Medicine Beijing 100035 China

AIM： To investigate the relationship between insulin resistance （IR）/β-cell dysfunction and diabetic retinopathy （DR） in Chinese patients with type 2 diabetes mellitus （T2DM）, and to explore further whether there were differences in the relationship among diabetic patients with higher and lower body mass index （BMI）. METHODS： Cross-sectional study. A total of 1466 subjects with T2DM were recruited in a local Desheng Community of urban Beijing from November 2009 to June 2012 for the cohort of Beijing Desheng Diabetic Eye Study. Standardized evaluation was carried out for each participant, including questionnaire, ocular and anthropometric examinations, and laboratory tests. Seven fields 30° color fundus photographs were used for DR grading according to the Early Treatment Diabetic Retinopathy Study protocols. Homeostatis Model Assessment （HOMA） method was employed for IR and β-cell function assessment. RESULTS： After excluding those participants who were treated with insulin （n=352） or had missing data of fasting insulin （n=96）, and further excluding those with poor quality of retinal photographs （n=10）, a total of 1008 subjects were included for the final analysis, 406 （40.3%） were men and 602 （59.7%） were women, age ranging fiom 34 to 86 （64.87±8.28）y. Any DR （levels 14 and above） was present in 278 （27.6%） subjects. After adjusting for possible covariates, the presence of any DR did not correlate with HOMA IR [odds ratio （OR） 1.51, 95% confidence interval （Cl） 0.87-2.61, P=0.14] or HOMA β-cell （OR 0.71, 95%CI 0.40-1.26, P=0.25）. After stratification by BMI, the presence of any DR was associated positively with HOMA IR （OR 2.46, 95%CI： 1.18-5.12, P=0.016）, and negatively with HOMA β-cell （OR 0.40, 95%CI： 0.19-0.87, P=0.021） in the group of patients with higher BMI （225 kg/m2）. In the group of patients with lower BMI （〈25 kg/m2）, the presence of any DR was not associated with HOMA IR （OR 1.00, 95%C1： 0.43-2.33, P=I.00） or HOMA β-cell （OR 1.41, 95%CI： 0.60-3.32, P=

关键词： type 2 diabetes mellitus diabetic retinopathy insulin resistance β-cell function body mass index

The Beijing Desheng Diabetic Eye Study:rationale,design,methodology and baseline data

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 108-116页

作者： Yun-Yun Li Xiu-Fen Yang Hong Gu Xi-pu Liu Torkel Snellingen ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology and VisualSciences Key LaboratoryBeijing 100730China Department of Ophthalmology Friendship HospitalCapitalMedical UniversityBeijing 100050China Department of Ophthalmology Ningbo Medical TreatmentCenter Lihuili HospitalNingbo 315040Zhejiang ProvinceChina Sekwa Institute of Medicine Beijing 100035China

AIM： To present the rationale, design, methodology, and the baseline data of the Beijing Desheng Diabetic Eye Study（BDDES）, and to determine the prevalence of diabetic retinopathy（DR） and possible risk factors in patients with type 2 diabetes mellitus（T2DM） in an urban community of Beijing, ***： Community-based prospective cohort study of persons diagnosed with T2DM aged 30 y or older. The main variables of interest are the presence and progression of DR as determined by the standardized ETDRS grading of seven fields fundus photographs. The presence and severity of DR were analyzed for possible correlations to non-genetic and genetic ***： A total of 1438 participants with data available for analysis, the prevalence of any DR was 35.4%. The prevalence of mild non-proliferative diabetic retinopathy（NPDR）, moderate NPDR, severe NPDR, and proliferative diabetic retinopathy was 27.7%, 2.6%, 0.5% and 4.5%, respectively. By multiple logistic regression analysis, risk factors for the presence of any DR included male（P=0.031）, lower income level（P=0.011）, lower education background（P=0.022）, longer duration of diabetes（P=0.001）, younger age at diabetic onset（P=0.001）, higher systolic blood pressure（P=0.007）, higher glycosylated hemoglobin A1 c levels（P=0.001）, high albuminuria（P=0.03）, and use of insulin（P〈0.001）. For vision-threatening DR, four factors were significant： younger age at diabetic onset（P〈0.001）,higher systolic blood pressure（P=0.042）, high albuminuria（P〈0.001）, and use of insulin（P〈0.001）. CONCLUSION： The BDDES is the first large-scale ongoing cohort study of a Chinese urban population of persons with type 2 diabetes. Using standardized grading system comparable to large cohort studies from western populations, our baseline data shows that the prevalence of DR and major risk factors in this Chinese ethnic population are comparable to that found in the western population studies.

关键词： type 2 diabetes mellitus diabetic retinopathy risk factors cohort study

Visual impairment with possible macular changes after a high dose of sildenafil in a healthy young woman

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International Journal of Ophthalmology(English edition) 2018年第2期11卷 340-342页

作者： Yun-Yun Li Ya-Jie Yu Xi-pu Liu ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Sekwa Institute of Medicine Beijing 100035China

Dear Editor,Sildenafil citrate（Viagra;Pfizer Pharmaceuticals,New York,NY,USA）,a selective inhibitor of phosphodiesterase type 5（PDE-5）,is widely used for erectile *** clinical recommended dosage is 25 to 100 mg pe... 详细信息

关键词： Visual impairment with possible macular changes after a high dose of sildenafil in a healthy young woman PDE Figure

Association of candidate gene polymorphisms with diabetic retinopathy in Chinese patients with type 2 diabetes

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International Journal of Ophthalmology(English edition) 2020年第2期13卷 301-308页

作者： Wen-Ying Fan Hong Gu Xiu-Fen Yang Chong-Yang She Xi-pu Liu ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology and Visual Sciences Key LaboratoryBeijing 100730China Department of Ophthalmology Lihuili HospitalNingbo 315010Zhejiang ProvinceChina Department of Ophthalmology Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Ophthalmology Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Sekwa Eye Hospital Sekwa Institute of MedicineBeijing 100035China

●AIM:To investigate the association between a set of six candidate genes and the risk of diabetic retinopathy(DR)in an urban community cohort of Chinese patients with type 2 diabetes mellitus(T2 DM).●METHODS:A population-based cross-sectional *** diabetic subjects were recruited from an urban community in Beijing and categorized into groups of proliferative diabetic retinopathy(PDR),non-proliferative diabetic retinopathy(NPDR),or diabetic without any retinopathy(DWR)based on the fundus photography and duration of *** candidate genes,including advanced glycation end product specific receptor(AGER),aldose reductase(AKR1 B1),inducible nitric oxide synthase(i NOS),pigment epithelium derived factor(PEDF),tumor necrosis factor-alpha(TNF-α),and paraoxonase 1(PON1),were chosen based on Meta-analysis of genetic association studies for DR and biochemical pathways implicated in DR *** allele and genotype distribution of 21 functional singlenucleotide polymorphisms(SNPs)in those 6 candidate genes were investigated using MassARRAY genotyping system.●RESULTS:Among 1461 diabetic patients recruited from community,569 were selected in following genotyping analysis,including 97 patients with PDR,217 with NPDR,and 255 with *** the promoter variant rs1051993 in AGER gene,the distribution of allele and genotype in PDR group differed from that in DWR group(allele:P=0.011;genotype:P=0.01).Compared with DWR,patients with PDR had lower frequencies of heterozygous genotype GT(9.8%for DWR,1%for PDR,OR:0.10,95%CI:0.01-0.72)and minor allele T(4.9%for DWR,0.5%for PDR,OR:0.10,95%CI:0.01-0.75).In multivariate model,the distribution of genotype for rs1051993 in PDR group was significantly different from that in DWR group(GT vs GG:OR:0.07,95%CI:0.01-0.61,P<0.001).No association with DR was observed in other genotyped SNPs.●CONCLUSION:The data suggest a significant association of the promoter variant rs1051993 in AGER gene with PDR in Chinese cohort with T2DM.

关键词： polymorphisms diabetic retinopathy AGER AKR1B1 iNOS PEDF TNF-α PON1

C-reactive protein and diabetic retinopathy in Chinese patients with type 2 diabetes mellitus

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International Journal of Ophthalmology(English edition) 2016年第1期9卷 111-118页

作者： Xiu-Fen Yang Yu Deng Hong Gu Apiradee Lim Torkel Snellingen Xi-pu Liu ning-Li Wang Amitha Domalpally Ronald Danis ning-pu Liu Department of Ophthalmology the Friendship HospitalCapital Medical University Beijing 100050 China Beijing Tongren Eye Center Beijing Ophthalmology and Visual Sciences Key Laboratory Beijing Tongren HospitalCapital Medical University Beijing 100730 China Department of Mathematics and Computer Science Faculty of Science and Technology Prince of Songkla UniversityPattani Campus Muang Pattani 9400 Thailand Sekwa Research Institute Beijing 100088 China Fundus Photograph Reading Center Department of Ophthalmology and Visual Sciences University of Wisconsin Madison 53705 Wisconsin USA

AIM：To investigate the relationship between Creactive protein（CRP）and diabetic retinopathy（DR）in a cohort of Chinese patients with type 2 diabetes mellitus（T2DM）.·METHODS：Community-based observational cohort *** were 1131 participants recruited from November 2009 to September 2011 in Desheng community in urban *** diagnosed T2DM were recruited and underwent a standardized evaluation consisting of a questionnaire,ocular and anthropometric examinations and laboratory *** presence and severity of DR were assessed by seven fields 30°color fundus *** were then classified into groups with no DR,any DR,or vision-threatening *** was analyzed from serum of study subjects.·RESULTS：A total of 1007 patients with T2DM were included for analysis,including 408（40.5%）men and 599（59.5%）*** median CRP level was 1.5 mg/L for women and 1.1 mg/L for men（=0.004,OR 0.37,95%CI0.18-0.74）.After adjusting for possible covariates,higher levels of CRP were associated with lower prevalence of any DR（=0.02,OR 0.55,95%CI 0.35-0.89）,but not associated with vision-threatening DR（=0.62,OR 0.78,95%CI 0.28-2.14）.After stratification by sex,the inverse association between CRP and DR was found to be statistically significant in men（=0.006,OR 0.35,95%CI0.16-0.73）,but not in women（=0.58,OR 0.88,95%CI0.29-1.16）.·CONCLUSION：The data drawn from a Chinese population with T2DM suggest that increasing CRP levels may be inversely associated with development of DR.

关键词： type 2 diabetes mellitus C-reactive protein diabetic retinopathy inflammation

Measurement of macular pigment optical density among healthy Chinese people and patients with early-stage age-related macular degeneration

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International Journal of Ophthalmology(English edition) 2015年第6期8卷 1190-1195页

作者： Xue-Tao Ren Hong Gu Xu Han Jun-Yan Zhang Xue Li Xiu-Fen Yang Jun Xu Torkel Snellingen Xi-pu Liu ning-Li Wang ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical University Beijing Ophthalmology and Visual Sciences Key Laboratory Sekwa Eye Hospital Department of Ophthalmology the First Hospital of Tsinghua University

AIMTo measure the macular pigment optical density (MPOD) in healthy Chinese people and patients with early age-related macular degeneration (AMD).METHODSCross-sectional population based study. Demographic and lifestyle characteristics were ascertained by questionnaire. A food frequency questionnaire was completed for all participants. Participants underwent general physical and ophthalmic examinations and MPOD was measured by heterochromatic flicker photometry. Foveal architecture was measured by optical coherence *** of 225 participants (122 healthy and 103 early AMD) was 0.48±0.18. Patients with early AMD (0.52±0.19) tended to have higher MPOD levels than healthy people (0.47±0.17), but the difference was not statistically significant (P=0.06). Participants with carrot or corn oil intake every week tended to have higher levels of MPOD (P=0.002 and 0.008 respectively) while those with corn intake had relatively lower level of MPOD (P=0.01). MPOD increased with the center foveal thickness (P=0.01).CONCLUSIONOur findings show that there is no statistically significant association between MPOD and early AMD in the studied population. MPOD is related to center foveal thickness and diets would influence MPOD levels.

关键词： age-related macular degeneration diets foveal architecture macular pigment optical density

NUMERICAL ANALYSIS OF LEAKAGE FLOW THROUGH TWO LABYRINTH SEALS

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Journal of Hydrodynamics 2007年第1期19卷 107-112页

作者： WANG Wei-zhe LIU Ying-zheng JIANG pu-ning CHEN Han-ping School of Mechanical and Power Engineering Shanghai Jiaotong University Shanghai 200030 China Shanghai Turbine LTD Shanghai 200240 China

The leakage flow through two labyrinth seals, e.g the interlocking seal and the stepped seal, was numerically investigated. Preliminary calculation of the seal-cavity averaged pressure by using the one-dimensional control volume method showed favorable agreement with the experimental measurements. Subsequently, in-depth understanding of the fluid flow through the labyrinth seals was obtained by employing Computational Fluid Dynamics （CFD） and k -ε turbulence model, which resulted in a potential wealth of information like the streamline pattern, velocity vector field, and distribution of turbulent kinetic energy and static pressure. At the clearance of the seal the turbulent kinetic energy reached the peak value, while in the bulk region of the cavities it decayed fast. The static pressure rapidly dropped as the fluid flow went through the clearance; no distinct difference of the static pressure was inspected in the cavities. Also noted from the numerical results was that the stepped seal showed better sealing performance than the interlocking seal.

关键词： labyrinth seal leakage flow,turbomachinery Computational Fluid Dynamics (CFD)