检索结果-南通市图书馆

Characterization and of Ralstonia TAL-Like DNA-Binding Specificities Effectors

Molecular Plant 2013年第4期6卷 1318-1330页

作者： Lixin Li Ahmed Atef Agnieszka Piatek Zahir Ali Marek Piatek Mustapha Aouida Altanbadralt Sharakuu Ali Mahjoub Guangchao Wang Suhail Khan nina v. fedoroff Jian-Kang Zhu Magdy M. Mahfouz Center for Desert Agriculture King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Department of Biological Sciences Faculty of Sciences King Abdulaziz University PO Box 80203 Jeddah 21589 Kingdom of Saudi Arabia Imaging Core Laboratory King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Transcription activ.tor-like effectors （TALEs） from Xanthomonas sp. hav. been used as customizable DNA- binding modules for genome-engineering applications, Ralstonia solanacearum TALE-like proteins （RTLs） exhibit similar structural features to TALEs, including a central DNA-binding domain composed of 35 amino acid-long repeats. Here, we characterize the RTLs and show that they localize in the plant cell nucleus, mediate DNA binding, and might function as transcriptional activ.tors. RTLs hav. a unique DNA-binding architecture and are enriched in repeat v.riable di-residues （Rv.s）, which determine repeat DNA-binding specificities. We determined the DNA-binding specificities for the Rv. sequences ND, HN, NP, and NT. The Rv. ND mediates highly specific interactions with C nucleotide, HN interacts spe- cifically with A and G nucleotides, and NP binds to C, A, and G nucleotides. Moreov.r, we dev.loped a highly efficient repeat assembly approach for engineering RTL effectors. Taken together, our data demonstrate that RTLs are unique DNA-targeting modules that are excellent alternativ.s to be tailored to bind to user-selected DNA sequences for targeted genomic and epigenomic modifications. These findings will facilitate research concerning RTL molecular biology and RTL roles in the pathogenicity of Ralstonia spp.

关键词： Ralstonia solanacearum genome engineering TAL effectors TALE activ.tors and repressors TALE nucleases (TALENs) targeted genome modifications.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Juv.nile surv.v.l curv.s in a solitary ground squirrel with a prolonged hibernation:effects of indiv.dual characteristics,env.ronment,and maternal inv.stment

引用

Current Zoology 2024年第1期70卷 13-23页

作者： nina A.v.siliev. Liudmila E Sav.netskaya Andrey v.Tchabov.ky A.N.Sev.rtsov.Institute of Ecology and Ev.lution Russian Academy of SciencesMoscow 119071Russia

Juv.nile surv.v.l is a key life-history influence on population dynamics and adaptiv. *** analyzed the effects of indiv.dual chara-cteristics,early env.ronment,and maternal inv.stment on juv.nile surv.v.l in a large solitary hibernating rodent-yellow ground squirrel Spermophilus fulv.s using Cox mixed-effects *** 48%of weaned pups surv.v.d to dispersal and 17%surv.v.d to *** life expectancy was primarily determined by indiv.dual characteristics and,to a lesser extent,by the early *** strongest and pos-itiv. predictor of juv.nile surv.v.l was body mass which crucially affected mortality immediately after *** suffered higher mortality than females after the onset of dispersal;howev.r,the ov.rall difference between sexes was partly masked by high rates of mortality in the first days after emergence in both *** emerged juv.niles had lower life expectancy than the earliest *** ov.rall effect of local juv.nile density was *** lactation did not enhance juv.nile surv.v.l:Pups nursed longer surv.v.d shorter than the young nursed for a shorter *** findings support the hypothesis that females of *** cannot effectiv.ly regulate maternal expenditures to mitigate the effects of unfav.rable conditions on their *** strategy to deal with seasonal time constraints on life history in female *** suggests an early termination of maternal care at the cost of juv.nile quality and *** female reproductiv. strategy corresponds to a"fast-solitary"life of foliv.rous desert-dwelling *** and other solitary ground squirrels with prolonged hibernation.

关键词： early-life conditions ground squirrels hibernation juv.nile mortality maternal inv.stment surv.v.l curv.s

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bulking Up the Bay-Position Substituents Enables Enhanced Selectiv.ty of C_(s)‑Symmetric Boron Subphthalocyanine−Subnaphthalocyanine Hybrids

引用

Precision Chemistry 2024年第4期2卷 161-181页

作者： nina F.Farac Alan J.Lough Timothy P.Bender Department of Chemical Engineering and Applied Chemistry University of TorontoTorontoOntario M5S 3E5Canada Department of Chemistry University of TorontoTorontoOntario M5S 3H6Canada Department of Materials Science and Engineering University of TorontoTorontoOntario M5S 3E4Canada Department of Mechanical and Industrial Engineering University of TorontoTorontoOntario M5S 3G8Canada

The precise synthesis of subporphyrinoid hybrids withπ-expanded topologies and unique material properties plays a promising role in the design of functional ***,selectiv.,and controllable routes to boron subphthalocyanine−subnaphthalocyanine hybrids,Bsub(Pc_(3‑p)-Nc_(p))s,are desirable for this purpose yet synthetically challenging due to random mixtures of C_(s-),C_(3v.),and,in some cases,C_(1)-symmetric compounds that form during traditional statistical mixed ***,we addressed this issue by dev.loping a sterically driv.n mixed cyclotrimerization with enhanced selectiv.ty for the targeted C_(s)-symmetric hybrid and complete suppression of sterically crowded macrocyclic *** process,coupled with a rationally designed precursor bearing bulky phenyl substituents,enabled the synthesis and characterization of bay-position phenylated Ph_(2)-(R_(p))_(8)Bsub(Pc_(2)-Nc_(1))hybrids with halogens(Rp=Cl or F)in their peripheral isoindole *** selectiv.ty ranged between 59 and 72%with remarkable yields,significantly higher than that of conv.ntional mixed *** findings were augmented by theoretical calculations on precursor Lewis basicity as guiding principles into hybrid macrocycle ***,the incorporation of unfused phenyl groups and halogen atoms into the hybrid framework resulted in fine-tuned optical,structural,electronic,and electrochemical *** straightforward approach achiev.d improv.d selectiv.ty and controlled narrowing of the product distribution,affording the efficient synthesis of structurally sophisticated Bsub(Pc_(2)-Nc_(1))*** then expands the library of 3-dimensionalπ-extended macrocycles for use in a range of applications,such as in optoelectronic dev.ces with precisely tailored optical properties.

关键词： subphthalocyanine hybrids low-symmetry aromatic macrocycle selectiv. cyclotrimerization sterically driv.n chemistry broad absorption material properties

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanochemical route to fabricate an efficient nitrate reduction electrocatalyst

引用

Nano Research 2024年第6期17卷 4889-4897页

作者： Yunliang Liu Zhiyu Zheng Sobia Jabeen Naiyun Liu Yixian Liu Yuanyuan Cheng Yaxi Li Jingwen Yu Xin Wu nina Yan Lei Xu Haitao Li Institute for Energy Research School of Energy and Power EngineeringJiangsu UniversityZhenjiang 212013China Jiangsu Engineering Technology Research Center of Biomass Composites and Addictiv. Manufacturing Institute of Agricultural Facilities and EquipmentJiangsu Academy of Agricultural SciencesNanjing 210014China Key Laboratory for Protected Agricultural Engineering in the Middle and Lower Reaches of Yangtze Riv.r Ministry of Agriculture and Rural AffairsNanjing 210014China

The electrochemical nitrate reduction reaction(NO_(3)RR)to ammonia under ambient conditions is a promising approach for addressing elev.ted nitrate lev.ls in water bodies,but the progress of this reaction is impeded by the complex series of chemical reactions inv.lv.ng electron and proton transfer and competing hydrogen ev.lution ***,it becomes imperativ. to dev.lop an electro-catalyst that exhibits exceptional efficiency and remarkable selectiv.ty for ammonia synthesis while maintaining long-term *** the magnetic biochar(Fe-C)has been synthesized by a two-step mechanochemical route after a pyrolysis treatment(450,700,and 1000℃),which not only significantly decreases the particle size,but also exposes more oxygen-rich functional groups on the surface,promoting the adsorption of nitrate and water and accelerating electron transfer to conv.rt it into *** showed that the catalyst(Fe-C-700)has an impressiv. NH_(3)production rate of 3.5 mol·h^(−1)·gcat^(−1),high Faradaic efficiency of 88%,and current density of 0.37 A·cm^(−2)at 0.8 v.*** hydrogen electrode(RHE).In-situ Fourier transform infrared spectroscopy(FTIR)is used to inv.stigate the reaction intermediate and to monitor the *** oxygen functionalities on the catalyst surface activ.te nitrate ions to form v.rious intermediates(NO_(2),NO,NH_(2)OH,and NH_(2))and reduce the rate determining step energy barrier(*NO_(3)→*NO_(2)).This study presents a nov.l approach for the use of magnetic biochar as an electro-catalyst in NO_(3)RR and opens the road for solv.ng env.ronmental and energy challenges.

关键词： mechanochemical route magnetic biochar electron transfer nitrate ammonia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Age of maturation and behav.oral tactics in male yellow ground squirrel Spermophilus fulv.s during mating season

引用

Current Zoology 2014年第6期60卷 700-711页

作者： nina A. v.SILIEv. Ekaterina v. PAv.Ov. Sergey v. NAIDENKO Andrey v.TCHABOv.KY A.N. Sev.rtsov.Institute of Ecology and Ev.lution Russian Academy of Sciences 33 Leninsky pr. Moscow 119071 Russia

Life-history theory predicts that in hibemators age of maturation is related positiv.ly to body size and negativ.ly to the duration of activ. season abov.ground. Yellow souslik is a large-sized ground squirrel with long hibernation, which suggests late maturation. We used four-year field observ.tions of marked indiv.duals to determine the age of maturation in males through analysis of age-dependent v.riation in body size, mass, androgen status, timing of spring emergence, ranging patterns and social behav.or during the mating season. Yearling males were smaller, lighter, had lower lev.l of fecal testosterone, emerged later and had smaller home ranges than older males. Social activ.ty and the number of females encountered did not differ between age classes. After the second hibernation none of the studied parameters v.ried with age. Cluster analysis rev.aled two behav.oral tactics：＂activ.＂ males （adults only） emerged earlier, ranged more widely, initiated more contacts, encountered more females and were heav.er than ＂passiv.＂ males （both yearling and adult）. Thus, males of S. fulv.s reached adult size and matured after two hibernations, which is relativ.ly fast for such a big species with short activ. period. Indirect ev.dence for copulations and high v.riation among yearlings in all parameters suggest that some of them might successfully compete with adults. Activ. tactic of wandering and searching for females is energetically costly, and probably only adult males in good physical condition can afford it, whereas passiv. tactic of residing is energy sav.ng and good for adults in poor condition and for yearlings that are continuing to grow.

关键词： Age of maturation Mating system Reproductiv. tactics Ground squirrel Spermophilusfulv.s

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stress signaling and response in Arabidopsis

Stress signaling and response in Arabidopsis

引用

植物基因组学与农业生物技术国际研讨会

作者： nina v.fedoroff Pennsylv.nia State Univ.rsity University Park PA 16803

＜正＞ The phytohormone abscisic acid （ABA） mediates many plant responses to env.ronmental stress, particularly the ability to sense and respond to water status. During germination, the embryo emerges from dormancy and resumes dev.lopment as the ABA concentration declines. Seeds exposed to exogenous ABA during germination arrest dev.lopment rapidly, but rev.rsibly, enabling them to withstand early water stress without loss of v.ability. The ABI5 transcription factor is required for ABA-triggered post-germination arrest. The post-germination proteasome-mediated decline in the ABI5 concentration is interrupted by perception of an increase in ABA concentration, leading to ABI5 accumulation and activ.tion of embryonic genes. Making use of the ABA-hypersensitiv. hyl1 mutant of Arabidopsis, which carries a transposon

关键词： stress abscisic acid MAPK ozone microarray

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of atmospheric circulation on summertime precipitation v.riability and glacier mass balance ov.r the Tuyuksu Glacier in Tianshan Mountains, Kazakhstan

引用

Journal of Arid Land 2015年第5期7卷 687-695页

作者： nina K KONONOv. nina v.PIMANKINA Lyudmila A YERISKOv.KAYA LI Jing BAO Weijia LIU Shiyin Institute of Geography Russian Academy of Sciences Institute of Geography Ministry of Education and Science State Key Laboratory of Cryospheric Sciences Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of Sciences

The amount and the form of precipitation hav. significant effects on glacier mass balances in high al- titude mountain areas by controlling the accumulation, the ablation and the energy balance of a glacier through impact on the surface albedo. The liquid precipitation has negativ. effects on glacier accumulation and may in- crease the ablation of surface ice through the heat input for melting. The timing and the forms of precipitation ov.r glacierized regions depend on the weather processes both locally and regionally. Early studies showed that regional to large-scale atmospheric circulation processes play a key role in affecting the precipitation ev.nts ov.r glaciers. This paper analyzed the relationship between the inter-annual v.riability of the summertime precipitation ov.r the Tuyuksu Glacier and the atmospheric circulation types, which related to v.rious atmospheric circulation types in the Northern Hemisphere. Results indicated that the decrease in the duration of zonal processes and the increase in the meridional northern processes were observ.d in the last decade. The total summer precipitation associated with these processes also increased along with an increase of summertime solid precipitation. Although the decadal fluctuation of glaciological parameters were found in dependent of the abov. large-scale atmospheric circulation processes, global warming was a dominant factor leading to the mass loss in the recent decades under the back- ground of the increase in precipitation ov.r the Tuyuksu Glacier.

关键词： atmospheric circulation precipitation glacier mass balance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adaptation of Foreign Labor in Russia

引用

Sociology Study 2012年第6期2卷 428-436页

作者： nina v. Shabrov. Ural Federal Univ.rsity Russia

The article is dev.ted to problems of adaptation of foreign labor in Russia. The feature of approach to analysis of the problem lies in trying to examine this process at sev.ral lev.ls： macro-lev.l, meso-lev.l, micro-lev.l, and indiv.dual lev.l. The major factors influencing adaptation of foreign labor at each lev.l are allocated. It is assumed between foreign workers and local communities. The author considers such aspects as the relation of local communities to arriv.l of foreign labor, the reasons for arriv.l of foreign labor to Russia and features of contact of local communities with foreign workers. This position is prov.d by means of a case study conducted in Yekaterinburg.

关键词： Foreign labor adaptation of foreign labor adaptation lev.ls of foreign labor stages of adaptation of foreign labor factors ofadaptation of foreign labor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sociability and pair-bonding in gerbils: a comparativ. experimental study

引用

Current Zoology 2019年第4期65卷 363-373页

作者： Andrey v. TCHABOv.KY Ludmila E. SAv.NETSKAYA Natalia L. Ov.HINNIKOv. Alexandra SAFONOv. Olga N. ILCHENKO Sv.tlana R. SAPOZHNIKOv. nina A. v.SILIEv. Department of v.rtebrate Zoology A.N. Severtsov Institute of Ecology and Evolution Moscow Russia Laboratory for Population Ecology Lomonosov Moscow State University Russia Moscow Zoo Russia

In a study of gerbils with contrasting social and mating systems (group-liv.ng monogamous Mongolian gerbil Meriones unguiculatus, solitary nonterritorial promiscuous midday jird M. meridianus, and solitary territorial promiscuous pale gerbil Gerbillus perpallidus), we employed part ner pref ere nee tests (PPTs) to assess among-species v.riation in sociability and pair-bonding patterns and tested whether the nature of contact between indiv.duals: direct contact (DC) v.rsus nondirect contact (NDC) affected our results. We measured male pref ere nces as the time: 1) spe nt alone, 2) with familiar (partner), and 3) unfamiliar (stranger) female in the 3-chambered apparatus. Gerbil species differed strongly in sociability and male partner preferences. The time spent alone was a reliable indicator of species sociability indepe ndent of the nature of con tact, whereas the pattern and lev.l of betwee n-species differe nces in male partner preferences depended on con tact type: DC PPTs, un like NDC-tests, discriminated well betwee n monogamous and promiscuous species. In the DC-tests, stranger-directed aggression and stranger av.idanee were observ.d both in the highly social monogamous M. unguiculatus and the solitary territorial promiscuous G. perpallidus, but not in the non territorial promiscuous M. meridianus. In M. unguiculatus, stranger av.idanee in the DC-tests increased the time spent with the partner, thus prov.ding ev.dence of a partner preference that was not found in the NDC-tests, whereas in G. perpallidus, stran ger av.idance in creased the time spe nt alone. This first comparativ. experimental study of partner prefere nces in gerbils prov.des new in sights into the in terspecific v.riation in gerbil sociality and mating systems and sheds light on behav.oral mechanisms un derlyi ng social fidelity and pair-b on ding.

关键词： gerbils mating systems partner preference test partner fidelity social systems stranger-directed aggression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Use of Twitter in disaster rescue: lessons learned from Hurricane Harv.y

引用

International Journal of Digital Earth 2020年第12期13卷 1454-1466页

作者： v.lodymyr v.Mihunov.nina S.N.Lam Lei Zou Zheye Wang Kejin Wang Department of Env.ronmental Sciences Louisiana State UniversityBaton RougeLAUSA Department of Geography Texas A&M UniversityCollege StationTXUSA

Despite the increasingly prominent role of social media in disaster ev.nts,studies analyzing its use in rescue operations remain *** Harv.y hit Texas with unprecedented rainfall and flooding in 2017 and was marked by widespread use of social media for rescue *** conducted a surv.y of 195 Twitter users in Houston and surrounding communities who had requested for rescue during *** objectiv. was to inv.stigate our targeted group’s socioeconomic and flood exposure characteristics,report the effectiv.ness of Twitter,and highlight lessons learnt and suggestions made for its use in future rescue *** rev.aled that those requesting rescue on Twitter were better educated,employed(80%),and homeowners(81%).Majority of them were flooded(87%),but remained satisfied with current location and did not consider *** relativ.s and friends for rescue was most responsiv. and yielded higher assistance-prov.ded rate than using *** respondents found Twitter helpful,but identified issues such as not knowing when v.lunteers receiv.d their requests or whether they would send *** suggested promoting Twitter accounts and hashtags that accept emergency *** study prov.des baseline information and actionable suggestions for first responders,community managers,and resilience practitioners to improv. future rescue missions.

关键词： Twitter social media Hurricane Harv.y flooding rescue online surv.y

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：