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Influence of chelator and near-infrared dye labeling on biocharacteristics of dual-labeled trastuzumab-based imaging agents

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Chinese Journal of Cancer Research 2016年第3期28卷 362-369页

作者： Xuejuan Wang Melissa B Aldrich Zhi Yang nina zhou Qing Xie Chen Liu Eva Sevick-Muraca Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Nuclear MedicinePeking University Cancer Hospital&InstituteBeijing 100142China Center for Molecular Imaging the Brown Foundation Institute of Molecular MedicineUniversity of Texas Health Science Center at HoustonHouston TX 77030USA

Objective： To investigate the effect of fluorescent dye labeling on the targeting capabilities of 111 In- （DTPA）.-trastuzumab-（IRDye 800）m. Methods： Trastuzumab-based conjugates were synthesized and conjugated with diethylenetriaminepentaacetic acid （DTPA） at molar ratios of I, 2, 3 and 5 and with a fluorescent dye （IRDye 800CW） at molar ratios of 1, 3 and 5. Immunoreactivity and internalization were assessed on SKBR-3 cells, overexpressing human epidermal growth factor receptor 2. The stability in human serum and phosphate-buffered saline （PBS） was evaluated. The biodistribution of dual-labeled conjugates was compared with that of 111In-（DTPA）2-trastuzumab in a SKBR-3 xenograft model to evaluate the effect of dye-to-protein ratio. Results： All trastuzumab-based conjugates exhibited a high level of chemical and optical purity. Flow cytometry results showed that increasing dye-to-protein ratios were associated with decreased immunoreactivity. Stability studies revealed that the conjugate was stable in PBS, while in human serum, increased degradation and protein precipitation were observed with increasing dye-to-protein ratios. At 4 h, the percentages of internalization of dual-labeled conjugates normalized by dye-to-protein ratio （m） were 24.88%±2.10%, 19.99%±0.59%, and 17.47%±1.26% for ＂m＂ equal to 1, 3, and 5, respectively. A biodistribution study revealed a progressive decrease in tumor uptake with an increase in the dye-to-protein ratios. The liver, spleen and kidney showed a marked uptake with increased dye-to-protein ratios, particularly in the latter. Conclusions： With non-specific-site conjugation of the fluorescent dye with a protein based on imaging agent, the increase in dye-to-protein ratios negatively impacted the immunoreactivity and stability, indicating a reduced tumor uptake.

关键词： Influence near infrared dye trastuzumab human epidermal growth factor receptor 2 dual-labeled

A Modified T-test Feature Selection Method and Its Application on the HapMap Genotype Data

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Genomics,Proteomics & Bioinformatics 2007年第Z1期 242-249页

作者： nina zhou and Lipo Wang School of Electrical and Electronic Engineering,Nanyang Technological University,Singapore.

Single nucleotide polymorphisms (SNPs) are genetic variations that determine the differences between any two unrelated individuals. Various population groups can be distinguished from each other using SNPs. For instance,the HapMap dataset has four population groups with about ten million SNPs. For more insights on human evolution,ethnic variation,and population assignment,we propose to find out which SNPs are significant in determining the population groups and then to classify different populations using these relevant SNPs as input features. In this study,we developed a modified t-test ranking measure and applied it to the HapMap genotype data. Firstly,we rank all SNPs in comparison with other feature importance measures including F-statistics and the informativeness for assignment. Secondly,we select different numbers of the most highly ranked SNPs as the input to a classifier,such as the support vector machine,so as to find the best feature subset corresponding to the best classification accuracy. Experimental results showed that the proposed method is very effective in finding SNPs that are significant in determining the population groups,with reduced computational burden and better classification accuracy.

关键词： SNP modified t-test F-statistics SVM

领导-部属交换感知匹配对员工工作表现的影响

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经济与管理研究 2010年第7期31卷 67-76页

作者：周妮娜中山大学岭南学院

本文通过410个领导与部属的配对样本分析了领导-部属交换(LMX)的感知匹配及其对员工工作表现的影响。基于自评-他评概念模型,本文提出了LMX感知的四种匹配类型:双高匹配(高领导LMX/高部属LMX)、双低匹配(低领导LMX/低部属LMX)、员工高估... 详细信息

本文通过410个领导与部属的配对样本分析了领导-部属交换(LMX)的感知匹配及其对员工工作表现的影响。基于自评-他评概念模型,本文提出了LMX感知的四种匹配类型:双高匹配(高领导LMX/高部属LMX)、双低匹配(低领导LMX/低部属LMX)、员工高估(低领导LMX/高部属LMX)和员工低估(高领导LMX/低部属LMX)。分析结果表明:(1)双高匹配LMX对员工工作表现有正向影响;(2)双低匹配LMX对员工工作表现有负向影响;(3)员工高估LMX可以正向预测工作满意度和组织承诺,员工低估LMX可以正向预测工作绩效和组织公民行为。本文研究验证了LMX感知匹配模型在中国情境下的有效性。

关键词：领导-部属交换领导-部属交换感知匹配员工工作态度员工工作行为

Prognostic value of ^(18)F-fluorodeoxyglucose positron emission tomography using Deauville criteria in diffuse large B cell lymphoma treated with autologous hematopoietic stem cell transplantation

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Chinese Journal of Cancer Research 2019年第1期31卷 162-170页

作者： Zhitao Ying Lan Mi nina zhou Xuejuan Wang Zhi Yang Yuqin Song Xiaopei Wang Wen Zheng Ningjing Lin Meifeng Tu Yan Xie Lingyan Ping Chen Zhang Weiping Liu Lijuan Deng Jun Zhu Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of LymphomaPeking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Peking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Nuclear MedicinePeking University Cancer Hospital&InstituteBeijing 100142China

Objective: High-dose chemotherapy(HDC) followed by autologous hematopoietic stem cell transplantation(auto-HSCT) plays an important role in improving outcomes of diffuse large B cell lymphoma(DLBCL) patients.18 F-fluorodeoxyglucose(18 F-FDG) positron emission tomography(PET)/computed tomography(CT) has been widely accepted in response assessment and prediction of prognosis in DLBCL. Here, we report the value of 18 FFDG PET/CT pre-and post-HSCT in predicting outcomes of patients with ***: DLBCL patients who had PET/CT scan before and after HSCT were included. PET results were interpreted based upon Deauville criteria. The prognostic value of 18 F-FDG PET/CT in auto-HSCT was ***: Eighty-four patients were enrolled. In univariate analysis, pre-and post-HSCT PET findings were correlated with 3-year progression-free survival(PFS) [hazard ratio(HR)=4.391, P=0.001; HR=7.607, P<0.001] and overall survival(OS)(HR=4.792, P=0.008; HR=26.138, P<0.001). Patients receiving upfront auto-HSCT after firstline treatment had better outcomes than relapsed/refractory DLBCL patients(3-year PFS, P<0.001; 3-year OS,P<0.001). In the relapsed/refractory patients, pre-and post-HSCT PET findings were also associated with 3-year PFS(P=0.003 vs. P<0.001) and OS(P=0.027 vs. P<0.001). A significant correlation was observed between clinical response to chemotherapy before auto-HSCT and outcomes of patients in the entire cohort(3-year PFS, P<0.001;3-year OS, P<0.001) and in the subgroup of 21 patients with positive pre-HSCT PET(3-year PFS, P=0.084; 3-year OS, P=0.240). A significant association between survival and post-HSCT PET findings was observed in multivariate analysis(HR=5.168, P<0.001).Conclusions: PET results before and after HSCT are useful prognostic factors for DLBCL patients receiving HSCT. Patients who responded to chemotherapy, even those with positive pre-HSCT PET, are appropriate candidates for auto-HSCT.

关键词： Positron emission tomography computed tomography autologous hematopoietic stem cell transplantation high-dose chemotherapy diffuse large B cell lymphoma

Radioprotection of Astragalus polysaccharide(APS) Against Genomic DNA Damage of mesenchymal stem cell Induced by Radiation

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Radioprotection of Astragalus polysaccharide(APS) Against Ge...

Experimental Bone Marrow Failure in Mice Ameliorated by OCH via Tippling the Balance of Released Cytokines from Th1 to Th2

第十届全国免疫学学术大会

作者： Liying Zhang Yongqi Liu nina zhou Yun Su Jiaxin He Yue Yang Zhiwei Lu Chunlu Yan Key Laboratory of Dunhuang Medicine and Transformation(Gansu University of Traditional Chinese Medicine)at Provincial and Ministerial Level Lanzhou

Objective:To study the effects of Astragalus Polysaccharide(APS)on the proliferation level and the protective effect on DNA damage of uman bone marrow mesenchymal stem cells which induced by the X-ray ***:using CCK-8 assay screening of the the optimal concentration of Astragalus Polysaccharide(APS)which act on HMSC-bm cells;The irradiation group was treated with 2Gy doses of X radiation,dosing group with the best concentration of drug interfere in irradiated HMSC-bm;cytoplasmic split block micronucleus technology(referred to as CB micronucleus assay)to detect the HMSC-bm micronucleus after rate X ray irradiation;immunofluorescence test gammaH2AX immunofluorescence cluster set focus(FOCI).Results:CCK8 screening drug concentration experiments showed,the concentration of Astragalus Polysaccharide in50ug/ml which promotes the highest proliferation,it is the best concentration,compared with control group(0ug/ml),the difference is statistically ***:Astragalus polysaccharide has protective effect on cell genome DNA damage the X ray radiation induced.

关键词： ionizing radiation bone marrow mesenchymal stem cells DNA damage micronucleus

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Experimental Bone Marrow Failure in Mice Ameliorated by OCH ...

中华医学会第十七次全国儿科学术大会

作者： Xiaohong Qiao,Xiaotian Xie,Shayi Jiang,Wei Shi,Jinqing Tang,nina zhou Department of Paediatrics,School of Medicine,Tongji University

Background OCH was reported to stimulate natural killer T(NKT) cells to produce predominantly T helper type 2(T) *** present study was attempted to evaluate potential protection of OCH on acquired bone marrow failure syndromes(BMFS) in mice model. Methods BMSF in mice model was established by exposure to sublethal irradiation followed by infusion with 5×10 B6 lymph nodes *** were injected intraperitoneally(LP.) with either OCH or a-galactosylceramide(α-GC) at a dose of 100μg kg twice a week for two weeks after *** control mice were *** vehicle alone(10%dimethyl sulfoxide(DMSO) in PBS).Meanwhile,anti-IL-4 monoclonal antibody(mAb)(500 ug /animal) was I.P.2 hours prior to vehicle or OCH *** interferon-y(IFN-y) and interleukin-4(IL-4) levels in the serum were measured by enzyme-linked immunosorbent assay(ELISA).Colony-forming unit-granulocyte-macrophage(CFU-GM) by BM mononuclear cells was *** percentage of NKT cell in BM cells and intracellular cytokines were determined by flow cytometry. Results The treatment of OCH in vivo decreased the IFN-y/ IL-4 ratio in the serum,and increased the transformation of NKT cells into NKT2 *** treatment of OCH in vitro increased the colonies of CFU-GM. Conclusion Our data suggests that OCH ameliorated immune-mediated BMSF in CByB6F1 mice via activation of NKT cells,and shifting the balance from T to T.

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聚苯胺防腐蚀涂料研究进展

化工新型材料 2006年第2期34卷 21-24页

作者：周妮娜李侃社周安宁西安科技大学化学与化工学院西安710054

介绍了聚苯胺防腐涂料的研究进展及不同的防腐机理。综述了聚苯胺掺杂态和本征态的防腐性能,以及不同涂层体系的防腐性能。

关键词：聚苯胺防腐涂料防腐机理

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三例屯南甲骨缀合分歧评议

中国文字研究 2019年第1期29卷 27-33页

作者：周妮娜华东师范大学中国文字研究与应用中心

小屯南地甲骨,因考古属性明确、坑位集中等因素使其在甲骨缀合中占了相当比重。近年来,笔者在对屯南缀合进行全面搜集的时候,发现屯南2558+屯南4465+合集32848、屯南2691+合集4935(遥缀)和屯南2064+屯南2986三例出现了意见分歧。笔者在... 详细信息

小屯南地甲骨,因考古属性明确、坑位集中等因素使其在甲骨缀合中占了相当比重。近年来,笔者在对屯南缀合进行全面搜集的时候,发现屯南2558+屯南4465+合集32848、屯南2691+合集4935(遥缀)和屯南2064+屯南2986三例出现了意见分歧。笔者在依据骨缝边缘、贞问内容、占卜时间、行款走向和字形特征等基本要素对其进行验证的同时,更多的参照了甲骨材料本身的形态属性对这三例分歧进行了评议,作出正误判断。

关键词：屯南甲骨缀合分歧评议

企业动态能力影响创新绩效的路径——基于能力层次论的分析

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企业经济 2022年第7期41卷 13-22页

作者：周妮娜张林李培祥广西财经学院工商管理学院广西南宁530007 广西大学经济学院广西南宁530004 广州理工学院工商管理学院广东广州510540

企业如何提升创新绩效是应对要素约束和复杂环境的重要问题,动态能力理论提供了一种解决思路,能力层次论进一步将动态能力分为基础动态能力和高阶动态能力。通过战略性新兴产业和先进制造业197份样本的实证分析,研究结果表明:高阶联盟... 详细信息

企业如何提升创新绩效是应对要素约束和复杂环境的重要问题,动态能力理论提供了一种解决思路,能力层次论进一步将动态能力分为基础动态能力和高阶动态能力。通过战略性新兴产业和先进制造业197份样本的实证分析,研究结果表明:高阶联盟组合能力显著影响企业创新绩效,并通过基础动态能力对创新绩效产生积极影响。管理实践中,企业不仅要苦练内功,实现创新驱动发展,而且要培育高阶能力,促进创新要素集聚,还要通过强化自身的创新主体地位,组建创新联合体,实现对关键核心技术的创新突破。此外,政府也应充分发挥组织优势和积极引导作用,促进创新联合体的健康发展。

关键词：动态能力创新绩效联盟组合能力吸收能力创新联合体

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