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Comparative Study between Endometrial Resection and Electrocoagulation in Patients with Abnormal Uterine Bleeding

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Open Journal of Obstetrics and Gynecology 2017年第3期7卷 312-325页

作者： Leonardo Vieira Elias Daniel Spadoto-Dias nilton josé leite Flávia Neves Bueloni-Dias Gustavo Filipov Peres Carlos Roberto Padovani Rogério Dias Department of Gynecology and Obstetrics Botucatu Medical School S&atilde o Paulo State University—UNESP S&atilde o Paulo Brazil Department of Biostatistics Botucatu Institute of Biosciences S S&atilde o Paulo State University—UNESP S&atilde o Paulo Brazil The Gynecological Endoscopy and Family Planning Sector Botucatu Medical School Hospital S&atilde o Paulo State University—UNESP and Principal Investigator S&atilde o Paulo Brazil

Objective: To compare clinical outcomes between two first-generation endometrial ablation techniques. Design: Prospective comparative coorte. Setting: Tertiary public hospital, university teaching center. Seventy-three patients with abnormal uterine bleeding unresponsive to clinical treatment submitted to endometrial ablation from October 2011 to September 2013. Methods and Main Outcome Measures: Patients were assigned to either monopolar U-shaped electrode resection with rollerball electrocoagulation (group A, n = 36) or rollerball electrocoagulation alone (group B, n = 37). Mean follow-up length was 359 (280 - 751) and 370 days (305 - 766) in groups A and B, respectively. Bleeding pattern, associated symptoms, failure/success rates were assessed 30, 90, 180 and 360 days post-procedure. Findings: Patient characteristics were similar in both groups (P ≥ 0.05). Surgery duration (mean of 48.5 [±12.0] vs. 31.9 [±5.6] min, P Conclusions: Endometrial ablation using rollerball electrocoagulation alone may be considered safer than resection with rollerball electrocoagulation, which requires shorter surgical time and less distention medium, and is associated with lower postoperative vaginal discharge incidence. Success rate did not statistically differ between groups, but study parameters in absolute values and percents were superior in group B.

关键词： Uterine Bleeding Hysteroscopy Surgical Procedures Endometrial Ablation Techniques Patient Satisfaction

Quantitative alpha-defensin testing: Is synovial fluid dilution important?

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World Journal of Orthopedics 2022年第8期13卷 760-767页

作者： Rodrigo Calil Teles Abdo Riccardo Gomes Gobbi Chilan Bou Ghosson leite Sandra Gofinet Pasoto Elaine Pires Leon Ana Lucia Lei Munhoz Lima Eloisa Bonfa joséRicardo Pécora Marco Kawamura Demange Instituto de Ortopedia e Traumatologia Hospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSao Paulo 05403-010Brazil Orthopaedic Department HCor-Hospital do CoraçãoSao Paulo 04004-030Brazil Division of Rheumatology Hospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSao Paulo 05403-010Brazil

BACKGROUND Alpha-defensin has been widely studied for the diagnosis of periprosthetic joint infection(PJI).However,there is a lack of detailed information regarding the proper laboratory technique of the enzyme-linked immunosorbent assay(ELISA)method,such as sample *** To assess the influence of dilution in the synovial fluid during ELISA for the diagnosis of knee PJI;and determine which dilution presents a better *** Forty samples of synovial fluid from arthroplasty knees were included,17 in the infected group and 23 in the aseptic group,according to Musculoskeletal Infection Society ***,five synovial fluid samples from each group were assessed for quantitative analysis of alpha-defensin using *** dilution ratios(1:10,1:100,1:500,1:1000 and 1:5000)were tested based on the predetermined cutoff value of 5.2 mg/*** dilutions that performed better were used to compare the results of all *** For infected cases,a gradual increase in the dilution of synovial fluid samples led to an equivalent increase in alpha-defensin *** same was not observed in the aseptic *** 1:1000 and 1:5000 dilutions presented satisfactory results to differentiate infected and aseptic *** analyses were performed using 1:1000 and 1:5000 for all 40 *** 1:1000 dilution resulted in a sensitivity of 88.2%(95%CI,66%-98%)and specificity of 95.7%(95%CI,79%-99%),whereas the 1:5000 dilution presented a sensitivity of 94.1%(95%CI,73%-99%)and a specificity of 100%(95%CI,86%-100%).CONCLUSION The synovial fluid dilution had an important influence on the alpha-defensin ELISA *** of 1:5000 showed the best performance for the diagnosis of knee *** results of this study set the basis for a more reliable and reproducible alpha-defensin ELISA during the investigation of PJI,contributing to the expansion of this technique in different treatment centers worldwide.

关键词： Alpha-defensin Enzyme-linked immunosorbent assay Periprosthetic joint infection Laboratory findings

Understanding how environmental heterogeneity and elevation drives the distribution of woody communities across vegetation types within the campo rupestre in South America

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Journal of Mountain Science 2021年第5期18卷 1192-1207页

作者： Marcelo Leandro BUENO Vanessa leite REZENDE Luiza Fonseca A.DE PAULA João Augusto Alves MEIRA-NETO joséRoberto Rodrigues PINTO Andreza Viana NERI Vanessa PONTARA State University of Mato Grosso do Sul Laboratory of Macroecology and EvolutionMundo Novo79980-000Mato Grosso do SulBrazil Federal University of Lavras Department of BiologyEcology and Conservation SectorLavras37200-000Minas GeraisBrazil Federal University of Minas Gerais Department of GeneticsEcology and EvolutionBelo Horizonte31270-901PampulhaMinas GeraisBrazil Federal University of Viçosa Department of Plant BiologyLaboratory of Ecology and Evolution of PlantsCampus Universitário36570-900ViçosaMinas GeraisBrazil University of Brasília Department of Forest EngineeringBrasília70910-900Federal DistrictBrazil

The campo rupestre sensu lato is a vegetation type that occurs in South American mountains,supports a distinctive flora characterized by high rates of endemism,high herbaceous species richness and often-neglected but also species-rich of the arboreal *** aimed to investigate how environmental factors and elevation are associated with the distribution and diversity of woody species in different rupestrian vegetation types across South *** a database of 2,049 woody species from 185 sites across four vegetation types within the campo rupestre,we assessed how the vegetation types were grouped according to their floristic composition and number of shared indicator species,as well as by using different beta diversity *** most important variables from a set of 27 variables(***,geo-edaphic and climatic)explaining species distribution were identified using redundancy analysis(RDA)and variation partitioning *** distribution of vegetation types was related to both environmental and spatial fractions,with a set of 17 variables retained(***,grass cover and temperature seasonality as the most important variables).There was an association between the floristic composition of each vegetation type and the elevation *** the identified vegetation types are floristically related,they are distinguished by exclusive and habitat-specialist woody *** uniqueness of vegetation types should be considered in terms of complementarity for the conservation of campos rupestres.

关键词： Beta diversity Campo rupestre Elevational gradient Rupestrian vegetation Tropical mountain Variation partitioning

Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration

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Neural Regeneration Research 2018年第1期13卷 100-104页

作者： Marcela Fernandes Sandra Gomes Valente Rodrigo Guerra Sabongi Joao Baptista Gomes dos Santos Vilnei Mattioli leite Henning Ulrich Arthur Andrade Nery Maria josé da Silva Fernandes Division of Hand and Upper Limb Surgery Department of Orthopedics and Traumatology Universidade Federal de Sao Paulo Sao Paulo Brazil Department of Biochemistry Institute of Chemistry Universidade de Sao Paulo Sao Paulo Brazil Division of Neurosciences Department of Neurology and Neurosurgery Universidade Federal de Sao Paulo Sao Paulo Brazil

Studies have confirmed that bone marrow-derived mesenchymal stem cells （MSCs） can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs （BMSCs） is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs （ADSCs） have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups： sham （only sciatic nerve exposed）, Matrigel （MG; sciatic nerve injury ＋ intravenous transplantation of MG vehicle）, ADSCs （sciatic nerve injury ＋ intravenous MG containing ADSCs）, and BMSCs （sciatic nerve injury ＋ intravenous MG containing BMSCs） groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury,increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios （axonal diameter/fiber diameter ratios） in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment.

关键词： nerve regeneration mesenchymal stem cells adipose-derived mesenchmal stem cells sciatic nerve Matrigel sciatic functional index neural regeneration

Isoflavone and Mineral Content in Conventional and Transgenic Soybean Cultivars

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American Journal of Plant Sciences 2015年第13期6卷 2051-2059页

作者： Gabriela Rezende Costa nilton de Oliveira Couto e Silva joséMarcos Gontijo Mandarino Rodrigo Santos leite Nilson César Castanheira Guimaraes Roberto Goncalves Junqueira Renata Adriana Labanca Postgraduate Program in Food Science Faculty of PharmacyFederal University of Minas Gerais(UFMG)Belo HorizonteBrazil Laboratory of Metal Contaminants(LCM) Division of Health and Environmental Surveillance(DIVISA)Octavio Magalh&atildees Institute(IOM)Funda&ccedil&atildeo Ezequiel Dias(FUNED)Rua Conde Pereira CarneiroBelo HorizonteBrazil Brazilian Agricultural Research Corporation-Embrapa Soja LondrinaBrazil National Agricultural Laboratory/LANAGRO-Ministry of Agriculture Livestock and Food Supply/MAPAPedro LeopoldoBrazil

The objective of this study was to evaluate the differences in composition among six brands of conventional soybean and six genetically modified cultivars (GM). We focused on the isoflavones profile and mineral content questioning the substantial equivalence between conventional and GM organisms. The statement of compliance label for conventional grains was verified for the presence of genetic modified genes by real time polymerase chain reaction (PCR). We did not detect the presence of the 35S promoter in commercial samples, indicating the absence of transgene insertion. For mineral analysis, we used the method of inductively coupled plasma-optical emission spectrometry (ICP-OES). Isoflavones quantification was performed by high performance liquid chromatography (HPLC). The results showed no statistical difference between the conventional and transgenic soybean groups concerning isoflavone content and mineral composition. The concentration of potassium, the main mineral component of soy, was the highest in conventional soybeans compared to that in GM soy, while GM samples presented the highest concentrations of iron.

关键词： Glycine max Genetically Modified Organism Bioactive Compounds ICP-OES HPLC

Multi-objective forest harvesting under sustainable and economic principles

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林业研究:英文版 2023年第5期34卷 1379-1394页

作者： Talles Hudson Souza Lacerda Luciano Cavalcante de Jesus Franca Isáira leite e Lopes Sammilly Lorrayne Souza Lacerda Evandro OrfanóFigueiredo Bruno Henrique Groenner Barbosa Carolina Souza Jarochinski e Silva Lucas Rezende Gomide Suzano SA Sao JoséDos CamposSao PauloBrazil Institute of Agricultural Sciences Federal University of Uberlandia–UFUMonte CarmeloMinas GeraisBrazil Department of Forest Sciences Federal University of Lavras–UFLALavrasMinas GeraisBrazil Brazilian Agricultural Research Corporation–EMBRAPA Rio BrancoAcreBrazil Department of Systems and Automation Federal University of Lavras–UFLALavrasMinas GeraisBrazil

Selective logging is well-recognized as an effective practice in sustainable forest ***,the ecological efficiency or resilience of the residual stand is often in *** time depends on operational variables,diversity,and forest *** logging is excellent but is open to *** may be resolved by mathematical programming and this study integrates the economic-ecological aspects in multi-objective function by applying two evolutionary *** function maximizes remaining stand diversity,merchantable logs,and the inverse of distance between trees for harvesting and log landings *** Brazilian rainforest database(566 trees)was used to simulate our 216-ha *** log landing design has a maximum volume limit of 500 *** nondominated sorting genetic algorithm was applied to solve the main optimization *** parallel,a sub-problem(p-facility allocation)was solved for landing allocation by a genetic *** frontier analysis was applied to distinguish the gradientsα-economic,β-ecological,andγ-*** expected,the solutions have high diameter changes in the residual stand(average removal of approximately 16 m^(3) ha^(-1)).All solutions showed a grouping of trees selected for harvesting,although there was no formation of large clearings(percentage of canopy removal<7%,with an average of 2.5 ind ha^(-1)).There were no differences in floristic composition by preferentially selecting species with greater frequency in the initial stand for *** implies a lower impact on the demographic rates of the remaining *** methodology should support projects of reduced impact logging by using spatial-diversity information to guide better practices in tropical forests.

关键词： Amazon rainforest management Computational intelligence Multi-objective functions Evolutionary computing

Water availability drives gradients of tree diversity,structure and functional traits in the Atlantic-Cerrado-Caatinga transition,Brazil

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Journal of Plant Ecology 2018年第6期11卷 803-814页

作者： Marcela de Castro Nunes Santos Terra Rubens Manoel dos Santos Jamir Afonso do Prado Júnior josé Marcio de Mello josé Roberto Soares Scolforo Marco Aurélio leite Fontes Ivan Schiavini Aliny Aparecida dos Reis Inacio Thomaz Bueno Luiz Fernando Silva Magnago Hans ter Steege Departamento de Ciências Florestais Universidade Federal de LavrasCampus UniversitárioPO Box 3037LavrasMinas Gerais 37200-000Brazil Instituto de Biologia Universidade Federal de UberlândiaCampus Umuarama-Bloco 2DPO Box 593UberlândiaMinas Gerais 38400-902Brazil Applied Ecology and Conservation Lab Programa de Pós‐graduação Ecologia e Conservação da BiodiversidadeUniversidade Estadual de Santa CruzIlhéusBahia 45662-900Brazil Naturalis Biodiversity Center Vondellaan 55Postbus 95172300 RA LeidenThe Netherlands Coordenação de Botânica Museu Paraense Emílio GoeldiAv.Magalhães Barata 376PO Box 399BelémPara´66040-170Brazil

Aims Climate and soil are among the most important factors determin-ing variation in tree communities,but their effects have not been thoroughly elucidated to date for many vegetation *** this study,we evaluate how climate and soil gradients affect gradients of vegetation composition,species diversity and dominance,structure and functional traits(seed mass and wood density)using over 327000 trees in 158 sites distributed along environmental gradients in the transitions among the Atlantic forest,Cerrado and Caatinga in Minas Gerais State(MG),Brazil(nearly 600000 km2).Methods Gradients in species,genus and family abundance in addition to basal area,stem density,species diversity(Fisher’s alpha),domin-ance percentage,seed mass and wood density were correlated using multiple regressions with environmental variables,as summarized in four principal component analysis axes(two climatic-precipi-tation seasonality and temperature range-and two edaphic-soil fertility and soil moisture).Additionally,ordinary kriging maps were used to better illustrate the *** Findings Multiple regression models indicate that all variables but domin-ance percentage were affected by one or more of the environmental gradients,but the average R2 was low(26.25%).Kriging maps rein-forced the patterns observed in the regression *** seasonality and soil moisture gradients were the most important gra-dients affecting vegetation *** finding suggests that water availability is an important determinant of vegetation features in these vegetation transitions.

关键词： climate environmental gradient soil southeastern Brazil vegetation

Enamel microabrasion: An overview of clinical and scientific considerations

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World Journal of Clinical Cases 2015年第1期3卷 34-41页

作者： Núbia Inocencya Pavesi Pini Daniel Sundfeld-Neto Flavio Henrique Baggio Aguiar Renato Herman Sundfeld Luis Roberto Marcondes Martins josé Roberto Lovadino Débora Alves Nunes leite Lima Department of Restorative Dentistry Piracicaba Dental SchoolUniversity of CampinasPiracicabaSão Paulo 13414-903Brazil Department of Restorative Dentistry Araçatuba Dental SchoolSão Paulo State UniversityAraçatubaSão Paulo 16015-050Brazil

Superficial stains and irregularities of the enamelare generally what prompt patients to seek dental intervention to improve their smile. These stains or defects may be due to hypoplasia, amelogenesis imperfecta, mineralized white spots, or fluorosis, for which enamel microabrasion is primarily indicated. Enamel microabrasion involves the use of acidic and abrasive agents, such as with 37% phosphoric acid and pumice or 6% hydrochloric acid and silica, applied to the altered enamel surface with mechanical pressure from a rubber cup coupled to a rotatory mandrel of a lowrotation micromotor. If necessary, this treatment can be safely combined with bleaching for better esthetic results. Recent studies show that microabrasion is a conservative treatment when the enamel wear is minimal and clinically imperceptible. The most important factor contributing to the success of enamel microabrasion is the depth of the defect, as deeper, opaque stains, such as those resulting from hypoplasia, cannot be resolved with microabrasion, and require a restorative approach. Surface enamel alterations that result from microabrasion, such as roughness and microhardness, are easily restored by saliva. Clinical studies support the efficacy and longevity of this safe and minimally invasive treatment. The present article presents the clinical and scientific aspects concerning the microabrasion technique, and discusses the indications for and effects of the treatment, including recent works describing microscopic and clinical evaluations.

关键词： Dental bleaching Enamel microabrasion Enamel surface Esthetic treatment Fluorosis Hypoplasia

Social Rights and Work on Digital Platforms in Brazil:The Case of“Breque dos Apps”

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Journal of Sociology Study 2023年第3期13卷 126-140页

作者： Raphaela Magnino Rosa Portilho Ricardo joséleite de Sousa University of the State of Rio de Janeiro(UERJ) Brazil

The movement known as“Breque dos Apps”was a social movement of a striker nature,which took place in Brazil in July *** movement was organized by workers which provided services to delivery apps(like iFood,Loggi,Uber Eats and Rappi).They demanded better working conditions and social protection measures,since they were out of the protection of the Brazilian legal *** reason for this lies in the fact that there is a debate about the classification of these workers-the maximum expression of the gig economy-as employees under Brazilian *** a result,and with the exponential growth in the use of these applications due to the Covid-19 pandemic,thousands of workers were left on the fringes of any social *** study aims to analyze the aforementioned movement and its demands within the paradigm of social rights,especially the ones regarding collective representation,which are guaranteed by the Brazilian *** terms of methodology,the content analysis method will be applied,considering that the established objective presupposes the study of theoretical and legal texts that allow the structuring of concepts applicable to the *** research technique to be adopted will be the analysis of indirect documentation,that is,literature review,to be carried out through bibliographic and documentary research,focused on literature and legal texts related to the topic.

关键词： Breque dos Apps strike delivery apps digital platform workers social rights