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冠心病介入術後服用氯吡格雷治療出現不良心血管事件與CYp2C19基因多態性關係的薈萃分析

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镜湖医学 2018年第1期18卷 35-42,53页

作者：鍾桃娟柯于梵蕭湛潮梁逸倫李兆文澳門鏡湖醫院心血管内科中國

目的探討經皮冠狀動脈介入治療(percutaneous Coronary Intervent ion,pCI)術後應用氯吡格雷治療發生主要不良心血管事件(Major Adverse Cardiovascular Events,MACE)與攜帶CYp2C19*2和(或)*3基因者之間的關像,為pCI術後患者醢床應用氯吡格雷治療提供更進一-步認識。方法在The Cochrane Library、pubMed、EMbase、萬方、知網資料庫檢索相關文獻,由兩位研究員獨立進行文獻篩選和評價,探用RevMan5.2整理資料薈萃分析。結果納入39篇文獻,共33989例研究對象。薈萃分析結果顯示:與非攜帶組比較,攜帶組發生MACE(OR,1.48;9S%CI,1.24~1.76;pppp20.02)的風險高;但兩組發生心源性死亡.出血事件的風險無統計學意義。結論Cp2C19基因多態性與冠心病pCI術後口服氯吡格雷治療的患者出現MACE存在正相關,提示攜帶CYp2C19功能缺失基因者發生支架内血栓、非致死性心肌梗死、TLR/TVR的風險更高。

关键词：經皮冠狀動脈介入治療氯吡格雷細胞色素p450酶 CYp2C19 基因多態性不良心血管事件薈萃分析

维普期刊数据库评论

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世界核心医学期刊文摘（皮肤病学分册） 2005年第6期1卷 18-18页

作者： Chuh A.A.T. Zawar V. Wong W.C.W. lee A. 牛新武 Dept. of Comm. and Family Medicine Chinese University of Hong Kong Prince of Wales Hospital ShatinChina HongKong

previous studies on the association of smoking with acne vulgaris have reported conflicting results. The objective of this study was to investigate such an association. Our setting was three primary carepractices in HongKong and one primary care practice in India. patient characteristics in all four practices are similar. All medical records in these practices incorporate a section in which the smoking habits of each patient is routinely documented. We searched our database and retrieved 632 records of patients with acne seen in the previous 5 years. We also retrieved 632 records of age-andsex-matched controls. Fifty- three out of 379 male patients with acne and 25 out of 379 male controls were smokers (p= 0.001; OR, 2.3; 95% CI, 1.43.8). Six out of 253 female patients with acne and three out of 253 female controls were smokers (OR, 2.0; 95% CI, 0.5- 9.4). We conclude that smoking is likely to bear a positive correlation with acne for men. Our numbers are too small for a definite conclusion to be drawn for females.

关键词：痤疮患者寻常性痤疮吸烟习惯病历记录女性痤疮中所

E型沙眼衣原体重组主外膜蛋白疫苗对恒河猴的免疫效应

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中华皮肤科杂志 2012年第7期45卷 492-495页

作者：李宜儒姚卫锋盛彩虹冯斌展小飞李玲杰尤聪李艳飞刘原君齐蔓莉刘全忠天津医科大学总医院皮肤性病科天津性传播疾病研究所300052

目的探讨E型重组主外膜蛋白（rMOMp）对恒河猴诱导产生的特异性免疫应答效应。方法恒河猴分3组，每组2只，分别为佐剂蛋白组、佐剂组、对照组。各组均于第0（免疫起始周）、2、4周双侧肱三头肌注射蛋白疫苗、佐剂和磷酸盐缓冲液（pBS）... 详细信息

目的探讨E型重组主外膜蛋白（rMOMp）对恒河猴诱导产生的特异性免疫应答效应。方法恒河猴分3组，每组2只，分别为佐剂蛋白组、佐剂组、对照组。各组均于第0（免疫起始周）、2、4周双侧肱三头肌注射蛋白疫苗、佐剂和磷酸盐缓冲液（pBS）。末次免疫后2周，酶联免疫吸附试验（ELISA）检测恒河猴血清中沙眼衣原体特异性IgG抗体和干扰素γ（IFN-γ），以及阴道冲洗液中沙眼衣原体特异性sIgA抗体，M1Tr法检测恒河猴淋巴细胞特异性增殖反应，观察恒河猴的迟发型超敏反应，以及恒河猴血清抗体中和试验，同源攻击后免疫荧光检测衣原体。用SpSS14．0软件进行单向方差分析和LSD法进行组间多重比较。结果佐剂蛋白组恒河猴血清衣原体特异性IgG抗体A。值为1．718±0．213，淋巴细胞增殖指数为7．012±1．026，IFN-γ水平为（1086．000±121．730）ng／L，迟发型超敏反应皮丘直径为（11．000±2．134）mm；佐剂组分别为0．841±0．315、4．473±1．850、（409．000±53．440）ng／L、（3．000±0．914）mm；对照组分别为0．791±0．437、1．426±1．104、（162．000±48．046）ng／L、0。佐剂蛋白组各项指标与佐剂组和对照组比较，差异均有统计学意义。提示蛋白疫苗能诱导对沙眼衣原体血清E型株的特异性免疫应答。同源攻击后佐剂蛋白组衣原体持续阳性5周后转阴性，佐剂组及对照组持续阳性。结论沙眼衣原体rMOMp能刺激恒河猴产生特异性的体液和细胞免疫应答，能诱导恒河猴产生抗沙眼衣原体保护效应。

关键词：衣原体沙眼细菌外膜蛋白质类疫苗动物实验

水稻HD-Zip基因OsHox24的表达与功能分析

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分子植物育种 2019年第4期17卷 1061-1068页

作者：付亚雄刘思琪汪琨璧朱骞 Sadia Nadir 熊海波李伟孙明姬李梦婷周丽冯天李东宣陈丽娟云南农业大学稻作研究所昆明650201 科技大学化学系巴基斯坦巴努28100 云南农业大学省部共建云南生物资源保护与利用国家重点实验室昆明650201

同源异型-亮氨酸拉链(homeodomain-leucine-zipper, HD-Zip)转录因子参与高等植物形态建成、生长发育和环境中各种逆境的调控。本研究旨在利用融合蛋白表达、RNA干扰载体(p OsHox24:GUS, pCaMV35S:OsHox24-EGFp, pCaMV35S:OsHox24-RNAi)遗传转化等分子生物学方法,解析水稻中同源异型域亮氨酸拉链转录因子OsHox24在水稻生殖生长过程中的时空表达特性及其生物学功能。生物信息学分析表明,Os-Hox24基因在水稻第2号染色体上,具有2个外显子,1个内含子,编码的蛋白质由261个氨基酸组成,在该蛋白第61～121位氨基酸残基位置处有一个典型的HB类转录因子的DNA结合区(DNA-binding domain)。系统进化分析表明该基因与OsHox22同源性很高。GUS组织染色与RT-pCR实验显示OsHox24在水稻根尖、茎节和节间、叶鞘及不同时期的幼穗中均有表达。洋葱内表皮细胞亚细胞定位显示OsHox24在细胞膜和细胞核中表达。与野生型植株相比,RNA干扰植株的结实率显著降低。本研究初步探明由OsHox24形成的转录因子参与了水稻的生殖发育的调控过程,为进一步探索水稻HD-Zip转录因子功能和育种方面的应用研究提供科学依据。

关键词： HD-Zip转录因子基因表达 OsHox24 功能分析

应用冰冻保存的人体卵巢组织行自体异位移植以恢复卵巢功能

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世界核心医学期刊文摘（妇产科学分册） 2005年第4期1卷 37-37页

作者： Kim S.S. Hwang I.-T. lee H.-C. 刘淑娟

To investigate ovarian function after heterotopic autotransplantation of human ovarian tissue banked at -196°C. A clinical case study. University medical center. A 37-year-old woman with cervical cancer (stage Ib). Frozenthawed human ovarian tissue was transplanted to two different heterotopic sites. Ovarian function of the grafts was monitored sequentially by blood sampling for the hormonal profiles and by ultrasound. The hormonal profile remained at the postmenopausal level until 10 weeks after transplantation. By 14 weeks, the return of ovarian function was evidenced by the elevation of the serum E2 level (57.5 pg/mL). While monitoring hormonal profiles every 2 days for 5 weeks, we observed the LH surge (69.8 IU/L) followed by the elevation of the p4 concentration (9.6 ng/mL), suggesting presumptive ovulation. The ultrasound revealed a dominant follicle on the rectus muscle in the abdominal site. However, there was no sign of follicle development in the breast site. Ovarian function ceased around 28 weeks after transplantation. Heterotopic autotransplantation of cryobanked human ovarian tissue can be a practical strategy for restoration of ovarian function after cancer treatment. As a site for transplantation, a space between the rectus sheath and the rectus muscle appeared to be effective.

关键词：卵巢组织冰冻保存异位移植腹直肌鞘右卵巢卵泡发育病例研究大学医学中心周左可行性策略

宫颈癌放疗后人类表皮生长因子受体家族(EGFR,HER2,HER3,HER4)、磷酸化Akt(p-Akt)与临床预后的相关性

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世界核心医学期刊文摘（妇产科学分册） 2006年第2期2卷 52-52页

作者： lee C.M. Shrieve D.C. ZempolichK.A. D.K. Gaffney 张丽娟 Department of Radiation Oncology Huntsman Cancer Hospital University of Utah Medical Center 1950 Circle of Hope Salt Lake City UT 84112 United State

Objective. To investigate prognostic significance of and correlations between HER1 (EGFR), HER2 (c-erb-B2), HER3 (c-erb-B3), HER4 (c-erb-B4), and phosphorylated Akt (p-Akt) in patients treated with radiation for cervical carcinoma. Methods. Fifty-five patients with stages I-IVA cervical carcinoma were treated with definitive radiotherapy. Tumor expression of each biomarker was quantitatively scored by an automated immunohistochemical imaging system. parametric correlations were performed between biomarkers. Univariate and multivariate analysis was performed with disease-free survival (DFS) and overall survival (OS) as primary endpoints. Results. Correlations were observed between expression of HER2 and HER4 (p = 0.003), and HER3 and HER4 (p = 0.004). Decreased HER2, HER4, and p-Akt expressions were significant for diminished DFS on univariate analysis (p = 0.04, p = 0.008, and p = 0.02, respectively). Increased EGFR, and diminished HER2, HER4, and p-Akt expression were significant or showed trends toward significance for diminished OS on univariate analysis (p = 0.07, p = 0.008, p = 0.09, and p = 0.08, respectively). After controlling for pretreatment factors, multivariate analysis revealed HER2 associated with improved OS (p = 0.05). Thesedata emphasize that significant correlations exist between the differential expression of various HER family receptors. Multivariate analysis revealed only increased HER2 expression associated with improved OS after controlling for pretreatment clinical factors. Conclusions. These data emphasize the importance of continued basic and translational research on the HER family of receptors in cervical carcinoma.

关键词： p-Akt 预后意义无瘤生存率临床因素转化性研究局部放射治疗单变量分析生物标记物原发病

社会心理因素与宫颈上皮内瘤样病变发展相关性的前瞻性研究

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世界核心医学期刊文摘（妇产科学分册） 2005年第12期1卷 38-38页

作者： Tiersma E.S.M. Van Der lee M.L. Garssen B. 李跃萍 Department of Obstetrics and Gynecology H3- P Leiden University Medical Center Postbox 9600 2300 RC Leiden Netherlands

Objective. To investigate the influence of psychosocial factors on the course of cervical intra-epithelial neoplasia (CIN). Methods. A group of 93 patients with CIN 1 or 2 was followed for 2.25 years by half-yearly colposcopy and cytology. Negatively-rated life events, social support, and coping style were studied in relation to distress during follow-up and in relation to time till progression and regression of CIN. Human papillomavirus (HpV) infection was controlled for as well as sick role bias caused by suspicion of having cervical cancer and distress due to the abnormal cervical smear. Results. During follow-up, progression was found in 20 patients (22% ), stable disease in 22 patients (24% ), and regression in 51 patients (55% ). Negatively-rated life events and lack of social support predicted distress longitudinally. No association was found between progression or regression of CIN and negatively-rated life events, lack of social support, coping style, and distress. Conclusion. We found no evidence that psychosocial factors influence the course of CIN.

关键词：上皮内瘤心理因素病变发展人乳头瘤病毒瘤样病变细胞学涂片阴道镜社会支持度心理应对不良应激

双眼外直肌后退术和单眼后退-缩短术对主视眼外斜视的矫治疗效比较

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世界核心医学期刊文摘（眼科学分册） 2006年第9期2卷 12-12页

作者： Jeoung J. W. lee M. J. Hwang J. - M. 王大江(译) Department of Ophthalmology Seoul National University College of Medicine Seoul South Korea 不详

pURpOSE:To compare the surgical outcomes of bilateral lateral rectus recession and unilateral recess-resect(RR)procedure on the nondominant eye for the patients of exotropia with a dominant fixating ***:prospective randomized comparative clinical ***:One hundred twenty-four patients of exotropia with an invariably fixating eye were enrolled to this *** were assigned randomly to two groups,those who underwent bilateral lateral rectus(BLR)-recessions(BLR group)or unilateral RR procedures on the nondominant eye(RR group);surgical outcomes were *** outcome was considered satisfactory if there was between 10 prism diopters of exophoria/tropia and 10 prism diopters of esophoria/tropia at 6 months after ***:In the BLR group,28 of the 58 patients(48.3%)had a satisfactory outcome,and 30 patients(51.7%)had *** was no case of overcorrection in the BLR *** the RR group,55 of the 66 patients(83.3%)had a satisfactory outcome;6 patients(9.1%)had recurrence,and 5 patients(7.6%)were overcorrected(p patients in the RR group had poor stereoacuity and constant exotropia before the *** cumulative probability of surgical success was significantly higher in the RR group than in the BLR group(p =.012,log rank test).CONCLUSIONS:In the patients with exotropia with a dominant eye,the unilateral RR procedure resulted in a better outcome than BLR recession ***,the overcorrection rate was significantly higher in the unilateral RR procedure group,especially in those patients with a poor preoperative stereopsis status and constant exotropia.

关键词：双眼外直肌疗效比较矫治效果主视眼后退术外斜视缩短术单眼

细胞通过Anillin和Mid1铆钉收缩环的机理

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科学新闻 2016年第1期 98-98页

作者： Lingfei Sun Ruifang Guan I-Ju lee Yajun Liu Mengran Chen Jiawei Wang Jian-Qiu Wu 陈柱成清华大学生命科学院

文章简介Anillin和Mid1分别是动物细胞和真菌在细胞分裂时把收缩环铆钉在细胞中央的关键脚手架蛋白。在这个研究工作中,科研人员报道了人源Anillin和裂殖酵母Mid1的结构与功能分析。综合各种数据表明,Anillin包含一个隐藏的C2结构域以... 详细信息

文章简介Anillin和Mid1分别是动物细胞和真菌在细胞分裂时把收缩环铆钉在细胞中央的关键脚手架蛋白。在这个研究工作中,科研人员报道了人源Anillin和裂殖酵母Mid1的结构与功能分析。综合各种数据表明,Anillin包含一个隐藏的C2结构域以及一个结合RhoA的结构域。与pH结构域一起,三个结合细胞膜的结构域协同地结合Rhoh和磷脂,从而把Anillin铆钉在细胞的分裂沟上。出乎意料的是,Mid1也是通过一个隐藏的C2结构域结合细胞膜。Mid1的二聚化使它对pI(4,5)p2磷脂有较高的亲和性和选择性。这些特点让它能够绕开对Rho(G蛋白)的依赖,稳定地铆钉在细胞的分裂面。

关键词：铆钉紧固件收缩环结构域 Mid1 Anillin 细胞