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Optimization of Acetic Acid Production Rate by Thermotolerant Acetobacterspp.

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Advances in Microbiology 2017年第11期7卷 749-759页

作者： Tahsina Islam Farzana Diba Roni Miah Ayesha Siddiqa Nafisa Azmuda Shamsun Nahar nihad Adnan Shuvra Kanti Dey Ali Azam Talukder Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Microbiology University of Dhaka Dhaka Bangladesh

Thermotolerant microorganisms were collected, identified and characterized under different physiological conditions from various rotten fruits in Bangladesh for vinegar production. Among the 15-isolates characterized previously, the strains F-1, F-3 and F-10 represented Staphylococcus, Bacillus and Acetobacter spp., respectively. After checking various parameters for growth, acetic acid production rate was optimized further. Among the 3-starins analyzed here, the strain F-10 gave maximum acetic acid (7.0 g/100 ml) at 37°C in 2% ethanol concentration. The strain F-10 is capable of producing high yield of acetic acid at relatively high temperature, which is an ideal condition for vinegar production, which may reduce the water cooling expenses as well as the risk of contamination.

关键词： Decomposed Fruits Materials Thermotolerant Microorganisms Acetobacter Spp. Acetic Acid Vinegar Production

Inexpensive Procedure for Measurement of Ethanol: Application to Bioethanol Production Process

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Advances in Microbiology 2017年第11期7卷 743-748页

作者： Roni Miah Ayesha Siddiqa Jamsheda Ferdous Tuli Noyon Kumar Barman Shuvra Kanti Dey nihad Adnan Mamoru Yamada Ali Azam Talukder Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Biological Chemistry Yamaguchi University Yamaguchi Japan

We have developed a spectrophotometric method for measurement of ethanol concentration in any unknown sample using a solvent tri-n-butyl phosphate [TBP, non-alcoholic solvent, density = 0.975 to 0.976, solubility in water = 0.028% (w/v)]. Solvent TBP separates ethanol from the non-hydrolyzed substrates that could interrupt the desired result by reacting with dichromate reagent. Oxidation of ethanol with dichromate reagent imparts blue green-colour to the solvent, which is easily detected by Spectrophotomer at 595 nm. Our established method showed similar results performed by relatively expensive Gas Chromatography (GC) method. In our present study we put forth a cheap alternate method for determining ethanol concentration in any aqueous solution.

关键词： Bioethanol Yeasts Saccharomyces cerevisiae

Intramedullary Metastasis of Medulloblastoma: A Case Report

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Open Journal of Modern Neurosurgery 2021年第2期11卷 90-96页

作者： Abderrazzak Bertal Alberic Fabrice Bocco Ismaïl Mahazou Asmaa Harrach nihad da Silva Mohamed Yassine Haouas Khadija Ibahioin Said Hilmani Mehdi Karkouri Abdelhakim Lakhdar Department of Neurosurgery Ibn Rochd University Hospital Center Casablanca Morocco Department of Hematology and Pediatric Oncology Ibn Rochd University Hospital Center Casablanca Morocco Department of Pathology Ibn Rochd University Hospital Center Casablanca Morocco Laboratory of Research on Neurologic and Neurosensorial Diseases and Handicap Faculty of Medicine Hassan II University Casablanca Morocco

Medulloblastoma (MB) is a malignant brain tumor with a usual potential for leptomeningeal spread. Intramedullary metastases of MB are rare and there are very few cases reported in the literature. Here, we report the case of an 18-year-old man with intramedullary spinal cord metastasis of MB occurring 9 years after the first diagnosis. The patient presented a 2-month history of progressive weakness in both lower limbs associated with urinary incontinence. Magnetic resonance imaging (MRI) demonstrated a large intramedullary spinal cord tumor extending from T10 to L1. The patient underwent surgical decompression and adjuvant therapy. Histological examination confirmed the diagnosis of classic MB metastasis. Postoperatively, the neurological status was stationary. Intramedullary metastasis of medulloblastoma is rare and difficult to manage with a poor prognosis. Comprehensive studies on the medulloblastoma dissemination mechanisms and clinical trials are needed to assess combined therapeutic approaches on metastases of MB.

关键词： Medulloblastoma Intramedullary Metastasis Classic Spinal Cord

Biochar potentially enhances maize tolerance to arsenic toxicity by improving physiological and biochemical responses to excessive arsenate

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Biochar 2023年第1期5卷 1222-1242页

作者： Md.Mezanur Rahman Ashim Kumar Das Sharmin Sultana Protik Kumar Ghosh Md.Robyul Islam Sanjida Sultana Keya Minhaz Ahmed Sheikh Arafat Islam nihad MdArifur Rahman Khan Mylea C.Lovell Md.Abiar Rahman S.M.Ahsan Touhidur Rahman Anik Pallavi Fnu Lam-Son Phan Tran Mohammad Golam Mostofa Department of Plant Soil ScienceInstitute of Genomics for Crop Abiotic Stress ToleranceTexas Tech UniversityLubbockTX 79409USA Department of Agroforestry EnvironmentBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Department of Agronomy Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Plant Pathology Division Bangladesh Rice Research InstituteGazipur 1701Bangladesh Department of Agriculture Bangabandhu Sheikh Mujibur Rahman ScienceTechnology UniversityGopalganj 8100Bangladesh Department of Energy Plant Research Laboratory Michigan State UniversityEast LansingMI 48824USA Plant Resilience Institute Michigan State UniversityEast LansingMI 48824USA Department of Biochemistry and Molecular Biology Michigan State UniversityEast LansingMI 48824USA

Metalloid pollution,including arsenic poisoning,is a serious environmental issue,plaguing plant productivity and quality of life ***,a carbon-rich material,has been known to alleviate the negative effects of environmental pollutants on ***,the specific role of biochar in mitigating arsenic stress in maize remains relatively ***,we elucidated the functions of biochar in improving maize growth under the elevated level of sodium arsenate(Na_(2)AsO_(4),AsV).Maize plants were grown in pot-soils amended with two doses of biochar(2.5%(B1)and 5.0%(B2)biochar Kg^(−1) of soil)for 5 days,followed by exposure to Na_(2)AsO_(4)(’B1+AsV’and’B2+AsV’)for 9 *** plants exposed to AsV only accumulated substantial amount of arsenic in both roots and leaves,triggering severe phytotoxic effects,including stunted growth,leaf-yellowing,chlorosis,reduced photosynthesis,and nutritional imbalance,when compared with control ***,biochar addition improved the phenotype and growth of AsV-stressed maize plants by reducing root-to-leaf AsV translocation(by 46.56 and 57.46%in‘B1+AsV’and‘B2+AsV’plants),improving gas-exchange attributes,and elevating chlorophylls and mineral levels beyond AsV-stressed *** pretreatment also substantially counteracted AsV-induced oxidative stress by lowering reactive oxygen species accumulation,lipoxygenase activity,malondialdehyde level,and electrolyte *** oxidative stress in‘B1+AsV’and‘B2+AsV’plants likely supported by a strong antioxidant system powered by biochar-mediated increased activities of superoxide dismutase(by 25.12 and 46.55%),catalase(51.78 and 82.82%),and glutathione S-transferase(61.48 and 153.83%),and improved flavonoid levels(41.48 and 75.37%,respectively).Furthermore,increased levels of soluble sugars and free amino acids also correlated with improved leaf relative water content,suggesting a better osmotic acclimatization mechanism in biochar-pretreated AsV-exposed plants.

关键词： Arsenic contamination Antioxidant defense system Biochar Gas-exchange attributes Nutrient dynamics Oxidative stress

Isolation, cytotoxic activity and phylogenetic analysis of Bacillus sp.bacteria associated with the red sea sponge Amphimedon ochracea

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Advances in Bioscience and Biotechnology 2012年第7期3卷 815-823页

作者： Haiam M. Aboul-Ela Mohamed A. Shreadah nihad M. Abdel-Monem Galila A. Yakout Rob W. M. van Soest Department of Biochemistry Faculty of Science Alexandria University Alexandria Egypt Department of Marine Zoology Netherlands Centre for Biodiversity Leiden The Netherlands Marine Chemistry Laboratory the National Institute of Oceanography and Fisheries Alexandria Egypt

Most of marine sponges harbor dense and diverse microbial communities of bioactivity importance. Four Gram positive bacterial cultures (HA-21, HA-68, HA- MS-105 and HA-MS-119) were isolated from the sponge Amphimedon ochracea, collected from the Red Sea coast of Egypt. Bacterial species were identified based on the phylogenetic analysis of the nucleotide sequences of their 16S rDNA genes. The Sequences similarity values of 98% - 100% to other strains in the NCBI database showed strong similarities with the 16S rDNA genes of firmicutes (Bacillus sp.). The four bacterial species were submitted to the GenBank database and had accession numbers of: HA-21 [JQ-768238];HA-68 [JQ751264];HA-MS-105 [JQ768239];HAMS-119 [JQ768240]. The cytotoxic activities of the bacterial isolates were tested against three established human cancer cell lines;HepG2 (hepatocellular carcinoma), HCT (colon carcinoma) and MCF-7 (breast carcinoma). The inhibitory effect on these cell lines, measured by MTT cell assay protocol, revealed promising cytotoxic activity of the four isolates (IC50 values (μg/mL) were: HA-21: 13.2, 9.3 and 12.2;HA-68: 10.42, 4.3 and 5.5;HA-MS-105: 46.9, 28.6 and 21.3;HAMS-119: 10.42, 6.3 and 22.1;respectively). The recovery of bacterial strains with cytotoxic activity suggests that marine invertebrates remain a rich source for the isolation of culturable isolates capable of producing novel bioactive secondary metabolites.

关键词： Sponge Associated Bacteria Bacillus Sp. Bioactive Metabolites Cytotoxic Activity Phylogenetic Analysis

Effectiveness of hepatitis B virus vaccination program in Egypt:Multicenter national project

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World Journal of Hepatology 2015年第22期7卷 2418-2426页

作者： Iman I Salama Samia M Sami Zeinab Nabil Ahmed Said Manal H El-Sayed Lobna A El Etreby Thanaa M Rabah Dalia M Elmosalami Amany T Abdel Hamid Somaia I Salama Aida M Abdel Mohsen Hanaa M Emam Safaa M Elserougy Amal I Hassanain Naglaa F Abd Alhalim Fatma A Shaaban Samia A Hemeda nihad A Ibrahim Ammal M Metwally Community Medicine Research Department National Research Center Child Health Department National Research Center Micro--biology and Immunology Department Faculty of Medicine (for girls)Al-Azhar University Pediatrics Department Faculty of MedicineAin-Shams University Dermatology and Venereology Department National Research Center Environmental and Occupational Medicine Department National Research Center

AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated ***:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory.

关键词： Hepatitis B virus Immunization Seroprotection Brea