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Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production

Systems Microbiology and Biomanufacturing 2022年第2期2卷 369-379页

作者： Aswathy Udayan nidhin sreekumar Muthu Arumugam Microbial Processes and Technology Division National Institute for Interdisciplinary Science and Technology(NIIST)CSIRIndustrial EstatePappanamcodeThiruvananthapuramKerala 695019India Academy of Scientifc and Innovative Research(AcSIR) Ghaziabad 201002India Accubits Invent Accubits Technologies Inc.ThiruvananthapuramKerala 695004India

Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical *** strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major *** the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product *** this study,the efect of plant growth regulators together with basal microalgal cultivation medium on biomass,total lipid,and EPA production was studied using the Plackett–Burman model and Response surface *** traditional one-factor-at-a-time optimization approach is laborious,time-consuming,and requires more experiments which makes the process and analysis more *** Designed PB model was found to be signifcant for biomass(396 mg/L),lipid(254 mg/L),and EPA(5.6%)production with a P value<*** major objective of this study is to formulate a medium for EPA production without compromising the growth ***,we had formulated a new media using RSM to achieve the goal and the signifcant variables selected were NaNO_(3),NaH_(2)PO_(4),and IAA and was found to be signifcant with 16.72%EPA production with a biomass production of 893 mg/L with a P value<*** formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica.

关键词： Microalgae Omega 3 fatty acids EPA Plackett–Burman Response surface methodology Plant growth regulators

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Lipid enhancement in microalgae by temporal phase separation:Use of indigenous sources of nutrients

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Chinese Journal of Chemical Engineering 2018年第1期26卷 175-182页

作者： nidhin sreekumar Ajit Haridas G.S.Godwin N.Selvaraju National Institute of Technology(NITC) CalicutIndia National Institute for Interdisciplinary Science and Technology(NIIST) Council of Scientific&Industrial Research(CSIR)ThiruvananthapuramIndia

Microalgae have been recommended as superior candidate for fuel production because of their advantages of higher photosynthetic efficiency, biomass & lipid productivity, and faster growth rate as compared to other energy crops. To meet up all these criteria, we have developed a continuous outdoor micro-algal raceway pond reactor(RPR) and a lab scale indoor tubular photo bioreactor(PBR) for biofuel production. An attempt to utilise indigenous sources of nutrients to improve the economics also revealed that micro-algal culturing can also be used as a mode of nutrient removal and water treatment. The photosynthetic rate and lipid production were enhanced by arresting daytime cell division and promoting night-time cell division. A 50% lipid improvement was observed for the particular algal consortia. Microscopic studies revealed that temporal phase separation could be achieved by adjusting nutrient distribution pattern. To monitor temporal phase separation, it is required to know DNA multiplication model. Quantification of g DNA in RPR confirmed that cell division happens during the night which positively affects the photosynthetic efficiency and lipid productivity of microalgae.

关键词： Temporal phase separation Lipid enhancement Microalgae Biodiesel

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Emerging industrial applications of microalgae:challenges and future perspectives

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Systems Microbiology and Biomanufacturing 2021年第4期1卷 411-431页

作者： Aswathy Udayan Ashutosh Kumar Pandey Pooja Sharma nidhin sreekumar Sunil Kumar CSIR-National Environmental Engineering Research Institute(CSIR-NEERI) Nehru MargNagpurMaharashtra 440020India Accubits Invent Accubits Technologies Inc.ThiruvananthapuramKerala 695004India

Microalgae are unicellular photosynthetic organisms that have been recently attracted potential interests and have applications in food,nutraceuticals,pharmaceuticals,animal feed,cosmetics,and biofertilizers *** are rich in a variety of high-value bioactive compounds which have potential benefits on human health and can be used for the prevention and curing of many disease *** scale-up and safety issues remain a major challenge in the commercialization of microalgal products in a cost-effective ***,techniques have been developed to overcome these challenges and successfully selling the products derived from microalgae as food,cosmetics and pharmaceutical *** are rich in many nutrients and can be used for the production of functional food and nutraceuticals,safety and regulatory issues are major concerns and extensive research is still needed to make microalgae a commercial success in the *** practical difficulties are involved in making the microalgal food industry commercially *** present review focuses on the industrial applications of microalgae and the challenges faced during commercial production.

关键词： Microalgae Functional foods Nutraceuticals Pharmaceuticals Cosmetics

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