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Wh.tefly feeding beh.vior and retention of a foregut-borne crinivirus exposed to artificial diets with.different ph.values

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Insect Science 2017年第6期24卷 1079-1091页

作者： Jaclyn S. Zh.u Angel Y. S. Ch.n Martin Drucker nicole h. lopez Alyssa Carpenter James C. K. Ng Department of Plant Path.logy and Microbiology and Center for Disease Vector Research University of California Riverside California USA INRA UMR BGPI Plant Pathogen Interactions Montpellier Cedex 5 France Barry University Miami Florida USA East Stroudsburg University E. Stroudsburg Pennsylvania USA

Transmission of plant viruses by ph.toph.gous h.mipteran insects encompasses complex interactions underlying a continuum of processes involved in virus acquisition, retention and inoculation combined with.vector feeding beh.vior. h.re, we investigated th. effects of dietary ph.on wh.tefly （Bemisia tabaci） feeding beh.vior and release of Lettuce infectious yellows virus （LIYV） virions retained in th. vector＇s foregut. Electrical penetration graph.analysis revealed th.t variables associated with.wh.tefly probing and ingestion did not differ significantly in ph.（4, 7.4, and 9） adjusted artificial diets. To investigate virus retention and release, wh.teflies allowed to acquire LIYV virions in a ph.7.4 artificial diet were fed ph.4, 7.4, or 9 virion-free artificial （clearing） diets. Immunofluorescent localization analyses indicated th.t virions remained bound to th. foreguts of approximately 20%-24% of vectors after th.y fed on each.of th. 3 ph. adjusted clearing diets. Wh.n RNA preparations from th. clearing diets were analyzed by reverse transcription （RT） nested-PCR and, in some cases, real-time qPCR, successful amplification of LIYV-specific sequence was infrequent but consistently repeatable for th. ph.7.4 diet but never observed for th. ph.4 and 9 diets, suggesting a weak ph.dependent effect for virion release. Viruliferous vectors th.t fed on each.of th. 3 ph.adjusted clearing diets transmitted LIYV to virus-free plants. Th.se results suggest th.t ch.nges in ph.values alone in artificial diet do not result in observable ch.nges in wh.tefly feeding beh.viors, an observation th.t marks a first in th. feeding of artificial diet by wh.tefly vectors; and th.t th.re is a potential causal and contingent relationsh.p between th. ph.in artificial diet and th. release/inoculation of foregut bound virions.

关键词： Crinivirus dietary ph inoculation noncirculative semipersistent transmission retention wh.tefly

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Blockade of Rh.-associated kinase prevents inh.bition of axon regeneration of periph.ral nerves induced by anti-ganglioside antibodies

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Neural Regeneration Research 2024年第4期19卷 895-899页

作者： Andrés Berardo Cristian R.Bacaglio Bárbara B.Báez Rubén Sambuelli Kazim A.Sh.ikh.Pablo h.h.lopez Laboratorio de Neurobiología Instituto de Investigación Médica Mercedes y Martin Ferreyra(INIMEC)-Consejo Nacional de Investigaciones Cientificas y Técnicas(CONICET)Universidad Nacional de Córdoba Departamento de Química Biológica-Centro de Investigaciones en Química Biológica de Córdoba(CIQUIBIC)-Consejo Nacional de Investigaciones Cientificas y Técnicas(CONICET) Facultad de Cs.QuímicasUniversidad Nacional de Córdoba Servicio de Anatomía Patológica Clínica Universitaria Reina FabiolaUniversidad Católica de Córdoba Department of Neurology University of Texas Medical School at Houston

Anti-ganglioside antibodies are associated with.delayed/poor clinical recovery in Guillain-Barrèsyndrome,mostly related to h.lted axon ***-linking of cell surface gangliosides by anti-ganglioside antibodies triggers inh.bition of nerve repair in in vitro and in vivo paradigms of axon *** effects involve th. activation of th. small GTPase Rh. A/ROCK signaling path.ays,wh.ch.negatively modulate growth.cone cytoskeleton,similarly to well stablish.d inh.bitors of axon regeneration described so *** aim of th.s work was to perform a proof of concept study to demonstrate th. effectiveness of Y-27632,a selective ph.rmacological inh.bitor of ROCK,in a mouse model of axon regeneration of periph.ral nerves,wh.re th. passive immunization with.a monoclonal antibody targeting gangliosides GD1a and GT1b was previously reported to exert a potent inh.bitory effect on regeneration of both.myelinated and unmyelinated *** results demonstrate a differential sensitivity of myelinated and unmyelinated axons to th. pro-regenerative effect of *** with.a total dosage of 9 mg/kg of Y-27632 resulted in a complete prevention of anti-GD1a/GT1b monoclonal antibody-mediated inh.bition of axon regeneration of unmyelinated fibers to skin and th. functional recovery of mech.nical cutaneous *** contrast,th. same dose sh.wed toxic effects on th. regeneration of myelinated ***,scale down of th. dosage of Y-27632 to 5 mg/kg resulted in a significant alth.ugh.not complete recovery of regenerated myelinated axons exposed to anti-GD1a/GT1b monoclonal antibody in th. absence of toxicity in animals exposed to only ***,th.se findings confirm th. in vivo participation of Rh. A/ROCK signaling path.ays in th. molecular mech.nisms associated with.th. inh.bition of axon regeneration induced by anti-GD1a/GT1b monoclonal *** findings open th. possibility of th.rapeutic ph.rmacological intervention targeting Rh. A/R

关键词： anti-ganglioside antibodies anti-glycan antibodies axon regeneration ganglioside Guillain-Barrésyndrome nerve repair ROCK Y-27632

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Th. association of diet quality and ph.sical activity with.cardiovascular disease and mortality in 85,545 older Australians:A longitudinal study

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Journal of Sport and h.alth.Science 2024年第6期13卷 841-850页

作者： Ding Ding Joe Van Buskirk Steph.nie Partridge Ph.lip Clare Edward Giovannucci Adrian Bauman nicole Freene Robyn Gallagh.r Binh.Nguyen Sydney Sch.ol of Public h.alth Faculty of Medicine and Healththe University of SydneyCamperdownNSW 2006Australia Ch.rles Perkins Centre the University of SydneyCamperdownNSW 2006Australia Sydney Local h.alth.District CamperdownNSW 2050Australia Sch.ol of h.alth.Sciences Faculty of Medicine and Healththe University of SydneyCamperdownNSW 2006Australia National Drug and Alcoh.l Research.Centre UNSW SydneySydneyNSW 2052Australia Departments of Nutrition and Epidemiology Harvard T.H.Chan School of Public HealthBostonMA 02115USA Faculty of h.alth University of CanberraCanberraACT 2617Australia Susan Wakil Sch.ol of Nursing and Midwifery the University of SydneyCamperdownNSW 2050Australia

Background:A quality diet and an active lifestyle are both.important cornerstones of cardiovascular disease(CVD)***,despite th.ir interlinked effects on metabolic h.alth.th. 2 beh.viors are rarely considered jointly,particularly with.n th. context of CVD *** examined th. independent,interactive,and joint associations of diet and ph.sical activity with.CVD h.spitalization,CVD mortality,and all-cause ***:CVD-free Australian participants aged 4574 years(n=85,545)reported ph.sical activity,diet,sociodemograph.c,and lifestyle ch.racteristics at baseline(20062009)and follow-up(20122015),and data were linked to h.spitalization and death.registries(03/31/2019 for CVD h.spitalization and all-cause mortality and 12/08/2017 for CVD mortality).Diet quality was categorized as low,medium,and h.gh.based on meeting dietary *** activity was operationalized as(a)total moderate-to-vigorous ph.sical activity(MVPA)as per guidelines,and(b)th. composition of MVPA as th. ratio of vigorous-intensity ph.sical activity(VPA)to total *** used a left-truncated cause-specific Cox proportional h.zards model using time-varying ***:During a median of 10.7 years of follow-up,6576 participants were admitted to th. h.spital for CVD and 6581 died from all causes(876 from CVD during 9.3 years).A h.gh.quality diet was associated with.a 17%lower risk of all-cause mortality th.n a low-quality diet,and th. h.gh.st MVPA category(compared with.th. lowest)was associated with.a 44%and 48%lower risk of CVD and all-cause mortality,*** interactions between diet and ph.sical activity were *** all outcomes,th. lowest risk combinations involved a h.gh.quality diet and th. h.gh.st MVPA *** for total MVPA,some VPA was associated with.furth.r risk reduction of CVD h.spitalization and all-cause ***:For CVD prevention and longevity,one sh.uld adh.re to both.a h.alth. d

关键词： Diet Ph.sical activity Cardiovascular disease Mortality Coh.rt studies

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Generation of lossy mode resonances(LMR)using perovskite nanofilms

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Opto-Electronic Advances 2024年第2期7卷 33-40页

作者： Dayron Armas Ignacio R.Matias M.Carmen lopez-Gonzalez Carlos Ruiz Zamarreño Pablo Zubiate Ignacio del Villar Beatriz Romero Electrical Electronic and Communications Engineering DepartmentPublic University of NavarraPamplona 31006Spain Institute of Smart Cities(ISC) Public University of NavarraPamplona 31006Spain Experimental Science and Tech.ology Sch.ol Rey Juan Carlos UniversityMostoles 28933Spain

Th. results presented h.re sh.w for th. first time th. experimental demonstration of th. fabrication of lossy mode resonance(LMR) devices based on perovskite coatings deposited on planar waveguides. Perovskite th.n films h.ve been obtained by means of th. spin coating tech.ique and th.ir presence was confirmed by ellipsometry, scanning electron microscopy, and X-ray diffraction testing. Th. LMRs can be generated in a wide wavelength.range and th. experimental results agree with.th. th.oretical simulations. Overall, th.s study h.gh.igh.s th. potential of perovskite th.n films for th. development of novel LMR-based devices th.t can be used for environmental monitoring, industrial sensing, and gas detection, among oth.r applications.

关键词： perovskite th.n films slab waveguide lossy mode resonance

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Exercise training mode effects on myokine expression in h.alth. adults:A systematic review with.meta-analysis

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Journal of Sport and h.alth.Science 2024年第6期13卷 764-779页

作者： Francesco Bettariga Dennis R.Taaffe Daniel A.Galvao Pedro lopez Ch.is Bish.p Anna Maria Markarian Valentina Natalucci Jin-Soo Kim Robert U.Newton Exercise Medicine Research.Institute Edith Cowan UniversityJoondalupWA 6027Australia Sch.ol of Medical and h.alth.Sciences Edith Cowan UniversityJoondalupWA 6027Australia Pleural Medicine Unit Institute for Respiratory HealthPerthWA 6009Australia Medical Sch.ol Faculty of Health&Medical SciencesUniversity of Western AustraliaPerthWA 6009Australia Grupo de Pesquisa em Exercicio para Populacoes Clinicas(GPCLIN) Universidade de Caxias do SulCaxias do SulRio Grande do Sul 95070-560Brazil London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK Department of Path.ph.siology and Transplantation University of MilanMilan 20133Italy Sch.ol of h.man Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4072Australia

Background:Th. benefits of exercise are well known;h.wever,many of th. underlying molecular mech.nisms are not fully *** muscle secretes myokines,wh.ch.mediate muscleorgan *** regulate satellite-cell proliferation and migration,inflammatory cascade,insulin secretion,angiogenesis,fatty oxidation,and cancer *** date,th. effects of different exercise modes(namely,aerobic and resistance exercise)on myokine response remain to be *** is crucial considering th. clinical implementation of exercise to enh.nce general h.alth.and wellbeing and as a medical ***:A systematic search.was undertaken in PubMed,MEDLINE,CINAh.,Embase,SPORTDiscus,andWeb of Science in April *** studies examining th. effects of a single bout of exercise on interleukin15(IL-15),irisin,secreted protein acidic and rich.in cysteine(SPARC),oncostatinM(OSM),and decorin were included.A random-effects meta-analysis was also undertaken to quantify th. magnitude of ***:Sixty-two studies were included(n=1193).Overall,exercise appeared to induce small to large increases in myokine expression,with.effects observed immediately after to 60 min post-exercise,alth.ugh.th.se were mostly not statistically *** aerobic and resistance exercise resulted in ch.nges in myokine levels,with.ut any significant difference between training modes,and with.th. magnitude of ch.nge differing across *** levels returned to baseline levels with.n 180 min to 24 h.***,owing to potential sources of h.terogeneity,most ch.nges were not statistically significant,indicating th.t precise conclusions cannot be ***:Knowledge is limited but expanding with.respect to th. impact of overall and specific effects of exercise on myokine expression at different time points in th. systemic *** research.is required to investigate th. effects of different exercise modes at multiple time points on myok

关键词： Myokine Resistance exercise Aerobic exercise Cytokine Systemic circulation

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C19MC miRNA-520G induces SP100 antiviral gene transcription and inh.bits melanin production in skin cutaneous melanoma

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Genes & Diseases 2024年第1期11卷 60-63页

作者： Goodwin G.Jinesh.Marian T.Smallin Nino Mtch.dlidze Ish. Godwin Marco Napoli nicole h.ckel Manali S.Ph.dke Avani A.Desh.ande Xiaobo Li Joh. h.Lockh.rt Jaden R.Baldwin Sueh.lay Acevedo-Acevedo Yifeng Gao Mich.lle A.Reiser Keiran S.M.Smalley Elsa R.Flores Andrew S.Broh. Department of Molecular Oncology H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Molecular Medicine Program H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Saveeth. Medical College ChennaiTamil Nadu 602105India Tumor Biology Department H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Sarcoma Department H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA

SP100 is an antiviral protein th.t restricts th. productive stage of h.man papillomavirus(h.V)and multiple oth.r viruses,and viruses in turn block sUMO-1-mediated stabilization of SP100 and promotes its degradation(Table S1).Interferon(IFN)signaling could still produce more SP100 th.ough.transcription to counteract viruses.1 Viruses also disable th. transcriptional up-regulation of SP100 to ach.eve persistent infection in h.sts.

关键词： SP100 melanoma stabilization

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Silk-based nerve guidance conduits with.macroscopic h.les modulate th. vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina h.omada Patrick h.imel Markus Kerbl LászlóGál Sylvia Nürnberger Barbara Sch.edl James Ferguson nicole Swiadek Xavier Monforte Joh.nnes C.h.inzel Antal Nógrádi Andreas h.Teusch.-Woller David h.rch.r Department Life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria Ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University Clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of h.nd- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Periph.ral nerve injuries induce a severe motor and sensory deficit. Since th. availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sough., including th. use of tubular nerve guidance conduits(tNGCs). h.wever, th. use of tNGCs results in poor functional recovery and central necrosis of th. regenerating tissue, wh.ch.limits th.ir application to sh.rt nerve lesion defects(typically sh.rter th.n 3 cm). Given th. importance of vascularization in nerve regeneration, we h.poth.sized th.t enabling th. growth.of blood vessels from th. surrounding tissue into th. regenerating nerve with.n th. tNGC would h.lp eliminate necrotic processes and lead to improved regeneration. In th.s study, we reported th. application of macroscopic h.les into th. tubular walls of silk-based tNGCs and compared th. various features of th.se improved silk^(+) tNGCs with.th. tubes with.ut h.les(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomograph. and h.stological analyses, we were able to prove th.t th. use of silk^(+) tNGCs induced th. growth.of blood vessels from th. adjacent tissue to th. intraluminal neovascular formation. A significantly h.gh.r number of blood vessels in th. silk^(+) group was found compared with.autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at th. distal coaptation point compared with.th. silk^(–) tNGCs at 7 weeks postoperatively. In th. 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth.of blood vessels th.ough.th. tubular walls of silk^(+) tNGCs, but with.ut improved functional recovery at 12 weeks postoperatively. Our data proves th.t macroporous tNGCs increase th. vascular supply of regenerating nerves and facilitate improved axonal regeneration in a sh.rt-defect model but not in a critical-size defect model. Th.s study suggests th.t furth.r optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion periph.ral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

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Outcomes of a non-randomised audit of single pigtail suture stents in urolith.asis management of Asian patients in Singapore

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Asian Journal of Urology 2024年第2期11卷 324-330页

作者： Ee Jean Lim Zh.n Wei Ch.o Resh.a Mangat Pradeep Durai Sarvajit Biligere Yiquan Tan Loh.h.n Yeung Marcus nicole Andrea Seet Li Ting Ch.n Tiong h.ng Stefania Ferreti Vineet Gauh.r Urology Department Singapore General HospitalSingapore Urology Department Tan Tock Seng HospitalSingapore Urology Department Ng Teng Fong HospitalSingapore Urology Department Azienda Ospedaliera-Uiversitaria(Hospital and University of Parma)ParmaItaly

Objective:Double-J(DJ)ureteric stents are commonly placed perioperatively for semirigid or flexible ureteroscopic renal *** is believed th.t lesser stent material with.n th. bladder mitigates stent-related *** study aimed to evaluate th. J-Fil ureteral stent,a single pigtail suture stent compared with.conventional DJ stent in relation to stent symptoms in an Asian population undergoing ureterorenal ***:Based on internal audit committee recommendation approval,th. records of 50 patients retrieved,available data of 41 patients wh. were prospectively enrolled into two groups(Group 1[J-Fil stent group],n=21 and Group 2[DJ stent group],n=20)between August 2020 to January 2021,were *** compared were nature of procedure,stone location and size,ease of deployment or removal,and complications.A modified universal stent symptom questionnaire was used to assess morbidity of stent symptoms with.n 48 h.of insertion and at ***:Both.groups h.d similar median age,distribution in male to female ratio,and stone *** overall median universal stent symptom questionnaire score at insertion was similar for bladder pain,flank or loin pain,and quality of life between Group 1 and 2;h.wever,at removal Group 1 fared significantly better th.n Group 2,especially for flank or loin pain and pain at *** groups h.d similar ease in insertion with.no h.spital ***:Our audit favoured th. single pigtail suture stent in Asian ureters in mitigating stent-related *** sh.wed a good safety profile with.easy deployment and *** promises a new standard in stenting.

关键词： Stent Ureteroscopy Pigtail suture stent Asianpatient Flexible ureteroscopic renal surgery

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Borderline resectable pancreatic cancer: Definitions and management

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World Journal of Gastroenterology 2014年第31期20卷 10740-10751页

作者： nicole E lopez Cristina Prendergast Andrew M Lowy Division of Surgical Oncology Department of Surgery Moores Cancer Center University of California San Diego

Pancreatic cancer is th. fourth.leading cause of cancer death.in th. United States. Wh.le surgical resection remains th. only curative option, more th.n 80% of patients present with.unresectable disease. Unfortunately, even among th.se wh. undergo resection, th. reported median survival is 15-23 mo, with.a 5-year survival of approximately 20%. Disappointingly, over th. past several decades, despite improvements in diagnostic imaging, surgical tech.ique and ch.moth.rapeutic options, only modest improvements in survival h.ve been realized. Neverth.less, it remains clear th.t surgical resection is a prerequisite for ach.eving longterm survival and cure. Th.re is now emerging consensus th.t a subgroup of patients, previously considered poor candidates for resection because of th. relationsh.p of th.ir primary tumor to surrounding vasculature, may benefit from resection, particularly wh.n preceded by neoadjuvant th.rapy. Th.s stage of disease, termed borderline resectable pancreatic cancer, h.s become of increasing interest and is now th. focus of a multiinstitutional clinical trial. h.re we outline th. h.story, progress, current treatment recommendations, and future directions for research.in borderline resectable pancreatic cancer.

关键词： Pancreatic cancer Borderline resectable pancreatic cancer Neoadjuvant Vascular resection Pancreaticoduodenectomy Wh.pple

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Welfare and Market Impacts of Food Safety Measures in Ch.na: Results from Urban Consumers' Valuation of Product Attributes

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Journal of Integrative Agriculture 2014年第6期13卷 1404-1411页

作者： David L. Ortega h. h.lly Wang nicole J. Olynk Widmar Department of Agricultural Foodand Resource EconomicsMichigan State University Department of Agricultural Economics Purdue University

Th.s study provides an economics assessment of various food safety measures in Ch.na. A ch.ice experiment approach.is used to elicit Ch.nese consumer preferences for various food safety attributes using data from a 2008 urban consumer survey. An alternative welfare calculation is used to model aggregate market impacts of select food safety measures. Our results sh.w th.t th. largest welfare gains are found in th. current government-run certiifcation program. Th. implementation of a th.rd-party certiifcation system, a traceability network and a product label would generate signiifcant value and would h.lp reduce current system inefifciencies in Ch.na. Th.s study builds on previous research.and provides an alternative approach.for calculating consumer valuation of safety and quality attributes th.t can be used to estimate aggregate economic and welfare impacts.

关键词： food safety consumer welfare food policy Ch.na

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