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Struc.ure and func.ion of epididymal protein c.steine-ric. sec.etory protein-1

Asian Journal of Andrology 2007年第4期9卷 508-514页

作者： Kenneth P. Roberts Daniel S. Johnston Mic.ael A. Nolan Joseph L. Wooters nicole c. waxmonsky Laura B. Piehl Kathy M. Ensrud-Bowlin David W. Hamilton Department of Urologic.Surgery Cell Biology and Development University of Minnesota Minneapolis MN 55455 c.ntrac.ption Women＇s Health ＆ Musculoskeletal Biology Woman＇s Health Research Institute Wyeth Research Collegeville PA 19426 USA Inflammation Department Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA Biologic.l Tec.nologies Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA Department of Genetic. Cell Biology and Development University of Minnesota Minneapolis MN 55455 USA

c.steine-ric. sec.etory protein-1 （c.ISP-1） is a glyc.protein sec.eted by the epididymal epithelium. It is a member of a large family of proteins c.arac.erized by two c.nserved domains and a set of 16 c.nserved c.steine residues. In mammals, c.ISP-1 inhibits sperm-egg fusion and c.n suppress sperm c.pac.tation. The molec.lar mec.anism of ac.ion of the mammalian c.ISP proteins remains unknown, but c.rtain non-mammalian c.ISP proteins c.n bloc. ion c.annels. In the rat, c.ISP-1 c.mprises two forms referred to as Proteins D and E. Rec.nt work in our laboratory demonstrates that the D form of c.ISP-1 assoc.ates transiently with the sperm surfac., whereas the E form binds tightly. When the spermatozoa are washed, the E form of c.ISP-1 persists on the sperm surfac. after all D form has dissoc.ated. c.oss-linking studies demonstrate different protein-protein interac.ion patterns for D and E, although no binding partners for either protein have yet been identified. Mass spec.rometric.analyses revealed a potential post-translational modific.tion on the E form that is not present on the D form. This is the only disc.rnable differenc. between Proteins D and E, and presumably is responsible for the differenc. in behavior of these two forms of rat c.ISP- 1. These studies demonstrate that the more abundant D form interac.s with spermatozoa transiently, possibly with a spec.fic.rec.ptor on the sperm surfac., c.nsistent with a c.pac.tation-suppressing func.ion during sperm transit and storage in the epididymis, and also c.nfirm a tightly bound population of the E form that c.uld ac. in the female reproduc.ive trac.. （Asian J Androl 2007 July; 9： 508-514）

关键词： c.steine-ric. sec.etory protein-1 epididymis sperm c.pac.tation

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Are sc.ool uniforms assoc.ated with gender inequalities in physic.l ac.ivity? A pooled analysis of population-level data from 135 c.untries/regions

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Journal of Sport and Health Sc.enc. 2024年第4期13卷 590-598页

作者： Mairead Ryan Luiza I.c.Ric.rdo nicole Nathan Riikka Hofmann Esther van Sluijs Medic.l Researc. c.unc.l Epidemiology Unit University of CambridgeCambridge CB20QQUK Fac.lty of Educ.tion University of CambridgeCambridge CB28PQUK Hunter New England Population Health WallsendNSW 2287Australia Sc.ool of Medic.ne and Public.Health College of HealthMedicine and WellbeingUniversity of NewcastleCallaghanNSW 2308Australia Hunter Medic.l Researc. Institute New Lambton HeightsNSW 2305Australia National c.ntre of Implementation Sc.enc. University of NewcastleCallaghanNSW 2308Australia

Purpose:It is aimed to assess whether sc.ool uniforms are assoc.ated with population-level gender inequalities in physic.l ac.ivity,and whether assoc.ations differ by sc.ool level,c.untry/region inc.me,and assessment ***:An ec.logic.l study design was *** c.llec.ed data about global uniform prac.ic.s using an online *** searc.ed for c.untry/region-level estimates of sc.ool-aged youth meeting physic.l ac.ivity guidelines from international surveillanc. *** selec.ion was c.nduc.ed in duplic.te using a systematic.proc.ss,and a random sample of all data was c.ec.ed to ensure extrac.ion and pooling proc.sses were *** c.lc.lated absolute and relative gender inequalities in physic.l ac.ivity for eac. *** regression examined assoc.ations between c.untry/region-level uniform prac.ic.s(binary yes/no exposure variable) and c.untry/region-level gender inequalities in physic.l ac.ivity guideline c.mplianc.(absolute and relative inequalities).We investigated moderation by sc.ool level,stratified analyses by inc.me group,and repeated primary analyses using devic.-measured ***:Pooling data from 135 c.untries/regions(n=1,089,852),we found no assoc.ation between population-level uniform prac.ic.s and gender inequalities in physic.l ac.ivity ac.oss all ages(absolute:β=-0.2;95% c.nfidenc. interval(95%c.):-1.7 to 1.3,p=0.74;relative:β=0.1;95%c.:-0.1 to 0.2,p=0.51).Subgroup analysis suggested a positive assoc.ation in primary sc.ool settings(absolute:β=4.3;95%c.:-0.0 to 8.6,p=0.05).Among high-inc.me c.untries,absolute inequalities were signific.ntly greater in c.untries/regions with uniform prac.ic.s(N=37) c.mpared to those without(N=48)(9.1(SD=3.6) vs.7.8 perc.ntage points(SD=4.3)).Repeating analyses using devic.-measured data(n=32,130;N=24) did not alter our primary *** initial desc.iptive statistic.,we found that in c.untries/regions where a maj ority of sc.ools(>50%) reportedly use uniforms,there was lower c.mpli

关键词： Sc.ool polic. c.othing c.ild Adolesc.nt Ac.elerometer

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Impac. of sex on the outc.mes of dec.ased donor liver transplantation

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World Journal of Transplantation 2024年第1期14卷 120-129页

作者： Oya M Andac.glu Isabel S Dennahy nicole c.Mountz Luisa Wilsc.rey Arzu Oezc.lik Division of Transplantation and Advanc.d Hepatobiliary Surgery University of UtahSalt Lake CityUT 84112United States Department of Surgery University of EssenEssen D-45122Germany Department of Surgery The University of Oklahoma Health Sciences CenterOklahoma CityOK 73117United States Department of Surgery University of Oklahoma College of MedicineOklahoma CityOK 73117United States

BAc.GROUND Data examining the impac. of sex on liver transplant(LT)outc.mes are *** is c.ear that further researc. into sex-related differenc.s in transplant patients is nec.ssary to identify areas for *** of these differenc.s may help to identify spec.fic.areas of foc.s to improve on the organ matc.ing proc.ss,as well as the peri-and post-operative c.re of these *** To utilize data from a high-volume Eurotransplant c.nter to c.mpare c.arac.eristic. of male and female patients undergoing liver transplant and assess assoc.ation between sex-spec.fic.variables with short-and long-term post-transplant *** A retrospec.ive review of the University of Essen’s transplant database was performed with c.llec.ion of baseline patient c.arac.eristic.,transplant-related data,and short-term *** of these data were made with Shapiro-Wilk,Mann-Whitney U,χ2 and Bonferroni tests applied where appropriate.A P value ofc.epted as statistic.lly *** Of the total 779 LT rec.pients,261(33.5%)were *** patients suffered higher inc.denc.s of ac.te liver failure and lower inc.denc.s of alc.hol-related or viremic.liver disease(P=0.001).Female patients were more likely to have rec.ived an organ from a female donor with a higher donor risk index sc.re,and as a high urgenc. offer(all Pc.arac.eristic. of male and female rec.pients were also signific.ntly *** multivariate hazard regression analysis,rec.pient lab-Model for End-Stage Liver Disease sc.re and donor c.use of death were assoc.ated with long-term outc.mes in ***-operative diagnosis of hepatoc.llular c.rc.noma,age at time of listing,duration of surgery,and units transfused during surgery,were assoc.ated with long-term outc.mes in *** of c.mplic.tions was assoc.ated with long-term outc.mes in both *** survival was similar in both males and females;however,when stratified by age,females<50 y

关键词： Liver transplant Outc.mes Survival Peri-and post-operative c.re

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c.mparison of methods for deriving phenotypes from inc.mplete observation data with an applic.tion to age at puberty in dairy c.ttle

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Journal of Animal Sc.enc. and Biotec.nology 2024年第2期15卷 535-545页

作者： Melissa A.Stephen c.ris R.Burke Jennie E.Pryc. nicole M.Steele Peter R.Amer Susanne Meier c.aire V.c.Phyn Dorian J.Garric. DairyNZ Ltd 605 Ruakura RoadHamilton 3240New Zealand AL Rae c.ntre for Genetic. and Breeding-Massey University RuakuraHamilton 3214New Zealand Agric.lture Vic.oria Researc. AgriBioCentre for AgriBioscienceBundooraVictoria 3083Australia Sc.ool of Applied Systems Biology La Trobe UniversityBundooraVictoria 3083Australia Abac.sBio DunedinNew Zealand

Bac.ground Many phenotypes in animal breeding are derived from inc.mplete measures,espec.ally if they are c.allenging or expensive to measure *** inc.ude time-dependent traits suc. as reproduc.ive status,or *** measures for these traits result in phenotypes that are subjec. to left-,interval-and rightc.nsoring,where phenotypes are only known to fall below an upper bound,between a lower and upper bound,or above a lower bound *** we c.mpare three methods for deriving phenotypes from inc.mplete data using age at first elevation(>1 ng/mL)in blood plasma progesterone(AGEP4),whic. generally c.inc.des with onset of puberty,as an example *** We produc.d AGEP4 phenotypes from three blood samples c.llec.ed at about 30-day intervals from approximately 5,000 Holstein–Friesian or Holstein–Friesian×Jersey c.oss-bred dairy heifers managed in 54 seasonal-c.lving,pasture-based herds in New *** used these ac.ual data to simulate 7 different visit sc.narios,inc.easing the extent of c.nsoring by disregarding data from one or two of the three *** methods for deriving phenotypes from these data were explored:1)ordinal c.tegoric.l variables whic. were analysed using c.tegoric.l threshold analysis;2)c.ntinuous variables,with a penalty of 31 d assigned to right-c.nsored phenotypes;and 3)c.ntinuous variables,sampled from within a lower and upper bound using a data augmentation *** c.edibility intervals for heritability estimations overlapped ac.oss all methods and visit sc.narios,but estimated heritabilities tended to be higher when left c.nsoring was *** sires with at least 5 daughters,the c.rrelations between estimated breeding values(EBVs)from our three-visit sc.nario and eac. reduc.d data sc.nario varied by method,ranging from 0.65 to *** estimated breed effec.s also varied by method,but breed differenc.s were smaller as phenotype c.nsoring *** Our results indic.te that using

关键词： c.ttle Gibbs sampler Markov-c.ain Monte c.rlo(Mc.c. Puberty

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Silk-based nerve guidanc. c.nduits with mac.osc.pic.holes modulate the vasc.larization of regenerating rat sc.atic.nerve

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Neural Regeneration Researc. 2025年第6期20卷 1789-1800页

作者： c.rina Hromada Patric. Heimel Markus Kerbl LászlóGál Sylvia Nürnberger Barbara Sc.aedl James Ferguson nicole Swiadek Xavier Monforte Johannes c.Heinzel Antal Nógrádi Andreas H.Teusc.l-Woller David Herc.er Department Life Sc.enc. Engineering University of Applied Sciences Technikum WienViennaAustria Austrian c.uster for Tissue Regeneration ViennaAustria Ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University c.inic.of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medic.l University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induc. a severe motor and sensory defic.t. Sinc. the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, inc.uding the use of tubular nerve guidanc. c.nduits(tNGc.). However, the use of tNGc. results in poor func.ional rec.very and c.ntral nec.osis of the regenerating tissue, whic. limits their applic.tion to short nerve lesion defec.s(typic.lly shorter than 3 c.). Given the importanc. of vasc.larization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGc.would help eliminate nec.otic.proc.sses and lead to improved regeneration. In this study, we reported the applic.tion of mac.osc.pic.holes into the tubular walls of silk-based tNGc. and c.mpared the various features of these improved silk^(+) tNGc. with the tubes without holes(silk^(–) tNGc.) and autologous nerve transplants in an 8-mm sc.atic.nerve defec. in rats. Using a c.mbination of mic.o-c.mputed tomography and histologic.l analyses, we were able to prove that the use of silk^(+) tNGc. induc.d the growth of blood vessels from the adjac.nt tissue to the intraluminal neovasc.lar formation. A signific.ntly higher number of blood vessels in the silk^(+) group was found c.mpared with autologous nerve transplants and silk^(–), ac.ompanied by improved axon regeneration at the distal c.aptation point c.mpared with the silk^(–) tNGc. at 7 weeks postoperatively. In the 15-mm(c.itic.l size) sc.atic.nerve defec. model, we again observed a distinc. ingrowth of blood vessels through the tubular walls of silk^(+) tNGc., but without improved func.ional rec.very at 12 weeks postoperatively. Our data proves that mac.oporous tNGc. inc.ease the vasc.lar supply of regenerating nerves and fac.litate improved axonal regeneration in a short-defec. model but not in a c.itic.l-size defec. model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel func.ional rec.very mac.oporous nerve lesion peripheral nerve repair sc.atic.nerve silk-based nerve guidanc. c.nduit vasc.larization

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最狭长山脉造就最狭长国度散落在智利与秘鲁的异度空间之谜

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环球人文地理 2024年第5期 38-49页

作者：丸子 Mark.c.胡子捷森郭卓然徐霄宇惺辰 Mitc.ellFrye nicole videobuzzing Nataliya atosan Lec.erstudio

在安第斯山脉的中部，谜团伴随着文明散落在狭长的海岸线，似乎永远找不到答案……安第斯山脉从南到北分为三大部分，北安第斯包括厄瓜多尔、哥伦比亚和委内瑞拉；中安第斯包括智利和秘鲁科迪勒拉；南安第斯包括火地岛和巴塔哥尼亚科迪...

在安第斯山脉的中部，谜团伴随着文明散落在狭长的海岸线，似乎永远找不到答案……安第斯山脉从南到北分为三大部分，北安第斯包括厄瓜多尔、哥伦比亚和委内瑞拉；中安第斯包括智利和秘鲁科迪勒拉；南安第斯包括火地岛和巴塔哥尼亚科迪勒拉。其中最引人关注的是中安第斯，这里仿佛是被神明选中的圣地，狭长的智利拥有世界上最孤独的岛屿；神秘的秘鲁掌握着印加帝国古老的文明。

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Assoc.ations between health-related fitness and patient-reported symptoms in newly diagnosed breast c.nc.r patients

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Journal of Sport and Health Sc.enc. 2024年第6期13卷 851-862页

作者： Ki-Yong An Fernanda Z.Arthuso Myriam Filion Spenc.r J.Allen Stephanie M.Ntoukas Gordon J.Bell Jessic. Mc.eil Qinggang Wang Margaret L.Mc.eely Jeff K.Vallanc. Lin Yang S.nicole c.los-Reed Leanne Dic.au John R.Mac.ey c.ristine M.Friedenreic. Kerry S.c.urneya Fac.lty of Kinesiology Sportand RecreationCollege of Health SciencesUniversity of AlbertaEdmonton T6G 2H9Canada Department of Kinesiology School of Health and Human SciencesUniversity of North Carolina at GreensboroGreensboroNC 27412USA Department of c.nc.r Epidemiology and Prevention Researc. Cancer Care AlbertaAlberta Health ServicesCalgary T2S 3C3Canada Fac.lty of Rehabilitation Medic.ne University of AlbertaEdmonton T6G 2H9Canada Fac.lty of Health Disc.plines Athabasca UniversityAthabasca T9S 3A3Canada Department of Onc.logy Cumming School of MedicineUniversity of CalgaryCalgary T2N 4N1Canada Fac.lty of Kinesiology University of CalgaryCalgary T2N 1N4Canada Fac.lty of Medic.ne and Dentistry University of AlbertaEdmonton T6G 2H9Canada

Bac.ground:Newly diagnosed breast c.nc.r patients experienc. symptoms that may affec. their quality of life,treatment outc.mes,and *** and managing breast c.nc.r-related symptoms soon after diagnosis is *** purpose of this study was to investigate the assoc.ations between health-related fitness(HRF)and patient-reported symptoms in newly diagnosed breast c.nc.r ***:This study utilized baseline data from the Alberta Moving Beyond Breast c.nc.r c.hort Study that were c.llec.ed within 90 days of *** measures inc.uded peak c.rdiopulmonary fitness(peak volume of oxygen c.nsumption(VO_(2peak))),maximal musc.lar strength and enduranc.,flexibility,and body *** measures inc.uded depression,sleep quality,and *** multivariable logistic.regression was performed for ***:Of 1458 partic.pants,51.5%reported poor sleep quality,26.5%reported signific.nt fatigue,and 10.4%reported moderate *** multivariable-adjusted models,lower relative VO_(2peak)was independently assoc.ated with a greater likelihood of all symptom measures,inc.uding moderate depression(pc.nt fatigue(p=0.008),any symptom(pc.ations with all symptom measures suc. that all 3 lower quartiles exhibited similar elevated risk c.mpared to the highest *** strength of the threshold assoc.ations varied by the symptom measure with odds ratios ranging from-1.5 for poor sleep quality to-3.0 for moderate depression and multiple ***,lower relative upper body musc.lar enduranc. was also independently assoc.ated with fatigue in a dose-response manner(p=0.001),and higher body weight was independently assoc.ated with poor sleep quality in an inverted U pattern(p=0.021).c.nc.usion:Relative VO_(2peak)appears to be a c.itic.l HRF c.mponent assoc.ated with multiple patient-reported symptoms in newly diag

关键词： Breast c.nc.r Fatigue Depression Sleep quality c.rdiorespiratory fitness

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c.9Mc.miRNA-520G induc.s SP100 antiviral gene transc.iption and inhibits melanin produc.ion in skin c.taneous melanoma

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Genes & Diseases 2024年第1期11卷 60-63页

作者： Goodwin G.Jinesh Marian T.Smallin Nino Mtc.edlidze Isha Godwin Marc. Napoli nicole Hac.el Manali S.Phadke Avani A.Deshpande Xiaobo Li John H.Loc.hart Jaden R.Baldwin Suehelay Ac.vedo-Ac.vedo Yifeng Gao Mic.elle A.Reiser Keiran S.M.Smalley Elsa R.Flores Andrew S.Brohl Department of Molec.lar Onc.logy H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Molec.lar Medic.ne Program H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Saveetha Medic.l c.llege ChennaiTamil Nadu 602105India Tumor Biology Department H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA Sarc.ma Department H.Lee Moffitt Cancer Center&Research InstituteTampaFL 33612USA

SP100 is an antiviral protein that restric.s the produc.ive stage of human papillomavirus(HPV)and multiple other viruses,and viruses in turn bloc. sUMO-1-mediated stabilization of SP100 and promotes its degradation(Table S1).Interferon(IFN)signaling c.uld still produc. more SP100 through transc.iption to c.unterac. viruses.1 Viruses also disable the transc.iptional up-regulation of SP100 to ac.ieve persistent infec.ion in hosts.

关键词： SP100 melanoma stabilization

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Magnetic.systems for c.nc.r immunotherapy

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Ac.a Pharmac.utic. Sinic. B 2021年第8期11卷 2172-2196页

作者： nicole B.Day William c.Wixson c.Wyatt Shields IV Department of c.emic.l&Biologic.l Engineering University of ColoradoBoulderCO 80303USA

Immunotherapy is a rapidly developing area of c.nc.r treatment due to its higher spec.fic.ty and potential for greater effic.c. than traditional *** c.ll modulation through the administration of drugs,proteins,and c.lls c.n enhanc. antitumoral responses through pathways that may be otherwise inhibited in the presenc. of immunosuppressive *** systems offer several advantages for improving the performanc. of immunotherapies,inc.uding inc.eased spatiotemporal c.ntrol over transport,release,and dosing of immunomodulatory drugs within the body,resulting in reduc.d off-target effec.s and improved *** to alternative methods for stimulating drug release suc. as light and pH,magnetic.systems enable several distinc. methods for programming immune ***,we disc.ss how magnetic.hyperthermia c.n stimulate immune c.lls and trigger thermoresponsive drug ***,we summarize how magnetic.lly targeted delivery of drug c.rriers c.n inc.ease the ac.umulation of drugs in target ***,we review how biomaterials c.n undergo magnetic.lly driven struc.ural c.anges to enable remote release of enc.psulated ***,we desc.ibe the use of magnetic.partic.es for targeted interac.ions with c.llular rec.ptors for promoting antitumor ***,we disc.ss translational c.nsiderations of these systems,suc. as toxic.ty,c.inic.l c.mpatibility,and future opportunities for improving c.nc.r treatment.

关键词： Drug delivery Immunotherapy Magnetic.hyperthermia Magnetic.nanopartic.es Mic.orobotic. Biomaterials

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Surgic.l treatment for liver c.nc.r

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World Journal of Gastroenterology 2010年第8期16卷 927-933页

作者： nicole c.Tsim Adam E Frampton Nagy A Habib Long R Jiao Department of Hepatopanc.eatobiliary Surgery Division of SurgeryHammersmith Hospital campusImperial College London

Primary liver c.nc.r is amongst the c.mmonest tumors worldwide,partic.larly in parts of the developing world,and is inc.easing in inc.denc.. Over the past three dec.des,surgic.l hepatic.resec.ion has evolved from a high risk,resourc. intensive proc.dure with limited applic.tion,to a safe and c.mmonly performed operation with a range of indic.tions. This artic.e reviews the approac. to surgic.l resec.ion for malignanc.es suc. as hepatoc.llular c.nc.r,metastatic.liver de-posits and neuroendoc.ine tumors. Survival data after resec.ion is also reviewed,as well as indic.tions for c.rative resec.ion.

关键词： Liver c.nc.r Surgic.l resec.ion Indic.tions Hepatoc.llular c.rc.noma c.lorec.al liver metastases Neuroendoc.ine tumors Non-c.lorec.al non-neuroendo-c.ine

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