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Analysis of Sodium and Potassium in Total Parenteral Nutrition Bags by ICP-MS and ICP-AES:Critical Influence of the Ingredients

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American Journal of Analytical Chemistry 2011年第5期2卷 573-581页

作者： nicolas marie Claire Verdier Barbara Le Bot Gwenola Burgot 不详

The compounding of total parenteral nutrition solutions (TPN) in the hospital pharmacy is a high-risk activity for which a quality assurance programme is necessary. The complexity of parenteral nutrition solutions containing almost 50 ingredients makes it difficult to measure each of them. On the other hand, the assay of electrolytes such as sodium and potassium is accepted as a quality marker for estimating compounding errors. Thus, the aim of this study was to estimate the influence of ingredients on the accuracy of assays of electrolytes. Experiments were performed with aqueous working simulated solutions of sodium and potassium prepared by the addition of each nutrient step by step, (dextrose, amino acids, lipids, vitamins and trace elements). Sodium and potassium levels were measured by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) and Atomic Emission Spectroscopy (ICP-AES). The performance of these methods was compared using statistical evaluations (t-test and Mann–Whitney test).The study highlights the interference of amino acids, vitamins and trace elements when measuring sodium, but no interference was noted during the measurement of potassium. To reduce the risk and to improve the quality of compounding, we used an automated compounding device but, even in this case, the acceptance criterion for sodium and potassium determination was not <10%.

关键词： Inorganic Cations Electrolytes Total Parenteral Nutrition Atomic Emission Spectrometry

Prevalence and Factors Associated with Anemia among People Living with Human Immunodeficiency Virus Followed at the Outpatient Treatment Centre of Panzi General Referral Hospital in Eastern Democratic Republic of Congo

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Journal of Biosciences and Medicines 2024年第3期12卷 259-274页

作者： Marlene Abedi Zalufa Jean-Paulin Mbo Mukonkole Aline Byabene Kusinza nicolas Vignier Dieudonné Bihehe Masemo David Willy Shamputi Omari Mukanga Lampard marie Hatem Department of Internal Medicine Panzi General Referral Hospital Bukavu Democratic Republic of Congo Evangelical University in Africa Bukavu Democratic Republic of Congo Statistics Department Panzi General Referral Hospital Bukavu Democratic Republic of Congo International Centre for The Advanced Research and Training-ICART Panzi Foundation Bukavu Democratic Republic of Congo Faculty of Medicine University of Kisangani Kisangani Democratic Republic of Congo Service des Maladies Infectieuses et Tropicales CHU Avicenne Bobigny France Sorbonne University Paris France School of Public Health Department of Social and Preventive Medicine Université de Montréal Montréal Canada

Introduction: Anemia in people living with human immunodeficiency virus (PLHIV) is a major health problem. Although anemia often responds to combination antiretroviral therapy, many patients remain anemic despite treatment, and such persistent anemia continues to adversely affect prognosis, regardless of drug response. Scientists have identified some of the factors involved. However, the mechanisms put in place have not been effective in overcoming them. Examples include the withdrawal of zidovudine from antiretroviral treatment lines, iron and folate supplementation, etc. Anemia is still a major concern in HIV-positive patients. The aim of this study is to assess the prevalence of anemia and its associated factors among PLHIV followed up at the outpatient treatment centre (CTA) of the Panzi General Reference Hospital (HGR) in South Kivu, Democratic Republic of Congo (DRC). Method: We conducted a cross-sectional, comparative study of 276 HIV-infected adults on antiretroviral therapy (ART) followed up at the CTA of Panzi HGR. Socio-demographic and nutritional parameters were collected using a survey questionnaire, and clinical assessment and nutritional status were performed at the centre. Hemoglobin, seric albumin and viral load determinations were performed at the HGRP laboratory. We constructed univariate and multivariate logistic regression models to assess factors associated with anemia in people living with HIV/AIDS. Results: We found a prevalence of anemia of 39.4%, including 4.1% severe anemia, 17.7% moderate anemia and 17.5% mild anemia. After multivariate adjustment, the factors associated with anemia in our PLHIV were: moderate undernutrition (aOR = 1.26;95% CI: 1.50 - 4.20;p = 0.001), severe undernutrition (aOR = 115.4;95% CI: 2.04 - 164.52;p = 0.021), hypoalbuminemia (aOR = 2.11;95% CI: 1.87 - 5.10;p = 0.004) and the lower degree of dietary diversity (aOR = 1.56;95% CI: 1.10 - 4.32;p = 0.034). Conclusion: The prevalence of anemia in PLHIV on ART is high.

关键词： Anemia HIV/AIDS Undernutrition PANZI Hospital

Morphological Characterization of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere According to the Age of Xanthosoma sagittifolium L. Schott Plants in the Field

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American Journal of Plant Sciences 2024年第3期15卷 161-179页

作者： Audrey Maguy Bengono Nyimiebolo Astride Carole Djeuani Hermann Désiré Mbouobda Antoine marie Kevin Tiki Theresa Akinimbom Moma Diobe Motassy Manuela Samuel Brice Adounga Christophe Fendju Pangueko Jones Nshanji Issofa Nguetrapouna Rose Theophine Derricka Djem Moutamal A. Ziem Amang Amang nicolas Niemenak Laboratory of Plant Physiology Department of Biological Sciences Higher Teacher Training College (HTTC) University of Yaoundé I Yaoundé Cameroon Department of Plant Biology Faculty of Sciences University of Yaoundé I Yaoundé Cameroon Department of Biology Higher Teacher Training College Bambili (HTTC) The University of Bamenda Bamenda Cameroon Departments of Microbiology Faculty of Science University of Yaoundé 1 Yaoundé Cameroon

The objective of this work was to carry out a morphological characterization of arbuscular mycorrhizal fungi in the rhizosphere of Xanthosoma sagittifolium L. Schott plants. The plant material used was the white and red cultivars of X. sagittifolium, belonging to age intervals of 3 - 6, 6 - 9, and 9 - 12 months. Three harvest sites were chosen in the Central Region of Cameroon. In each site, soil from the rhizosphere and plant roots was collected in a randomized manner. In the field, the agronomic parameters were evaluated. The physicochemical characteristics of the soils, the mycorrhization index, and the morphological characterization of the mycorrhizal types of each site were carried out. The results obtained show that the agronomic growth parameters varied significantly using the Student Newman and Keuls Test depending on the harvest sites. The soils’ pH in all sites was acidic and ranged between 4.6 and 5.8. The Nkometou site has a loamy texture while the Olembe and Soa sites have loam-clay-sandy and loam-clay textures respectively. The highest mycorrhization frequencies appeared at the Nkometou site, with 75 and 87.33% of the white and red cultivars plant roots at 6 - 9 and 3 - 6 months. The relative abundance of AMF arbuscular mycorrhizal fungal spores in the rhizosphere of X. sagittifolium plants varied with age and cultivar. There were 673 spores between 9 - 12 months in Nkometou in the red cultivar. Six AMF genera were identified in all the different soils collected: Acaulospora sp., Funneliformis sp., Gigaspora sp., Glomus sp., Scutellospora sp., and Septoglomus sp. The genus Glomus sp. was the most present at all age intervals in both cultivars.

关键词： Xanthosoma sagittifolium L. Schott Rhizosphere Harvest Site Arbuscular Mycorrhizal Fungi Diversity

Effect of Biostimulants Based on Natural Products on the Growth and Nutritional Value of Bean (Phaseolus vulgaris L.)

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American Journal of Plant Sciences 2024年第7期15卷 492-518页

作者： Aimé Giresse Tzeuton Flore Eliane Eyenga Arouna Meshuneke Gaston Mbang Elock Fabrice Damien Wassom Walter Jospin Timma Kom marie Paule Djam Kengoum Erica Cabrelle Damtse Damtse Landry Silatsa Fotsing William Asah Che Marlyne Josephine Mananga nicolas Niemenak Cécile Annie Ewané Laboratory of Plant Physiology and Biochemistry Higher Teachers Training College University of Yaound 1 Yaound Cameroon Department of Biochemistry Faculty of Sciences University of Yaound 1 Yaound Cameroon Laboratory of Food Sciences and Metabolism University of Yaoundé 1 Yaoundé Cameroon Laboratory of Phytoprotection and Valorisation of Genetic Resources of the Biotechnology Centre University of Yaound 1 Yaound Cameroon Department of Biological Sciences Higher Teachers Training College University of Yaound 1 Yaound Cameroon

Beans (Phaseolus vulgaris L.) are widely grown in Cameroon and play a key role in the fight against food insecurity, malnutrition and poverty. However, its cultivation encounters problems due to abiotic and biotic stresses, which leads to the use of synthetic fertilizers and pesticides, which cause significant damage to the environment and human health due to the presence of synthetics residues in the seeds, pods and in the leaves that are eaten. Promoting the use of natural products is becoming a necessity for organic and eco-responsible agriculture that limits contamination problems and improves people’s purchasing power. This study aims to assess the effect of biostimulants based on natural products on the growth and nutritional value of common bean (Phaseolus vulgaris L.). Bean seedlings from white variety (MEX-142) and red variety (DOR-701) were treated every seven days in the field from their pre-emergence, emergence and growth to their maturation under a randomized block experimental design. Six treatments and three repetitions with the biostimulants based on natural products and controls were thus performed and the agromorphological parameters were measured. After 120 days, the contents of growth biomarkers and defense-related enzymes were evaluated in leaves, while the contents of macromolecules, minerals and antinutrients were evaluated in seeds. These biostimulants significantly increased (P P < 0.0001) of antinutrients including oxalates, phytates, tannins and saponins in seeds compared to controls (T+ and T−). Treatment with biostimulants, in particular BS4, improves the performance of bean plants in the field as well as the biofortification of seeds regardless of the variety.

关键词： Phaseolus vulgaris L. Biostimulants Biofortification Growth and Defense Biomarkers Nutritional Value

Stimulatory Effect of Tithonya diversifolia-by Products on Plantain Banana Vivoplants in Nursery (A Review)

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American Journal of Plant Sciences 2024年第9期15卷 726-745页

作者： Cécile Annie Ewané Arouna Meshuneke Gaston Elock Mbang Fabrice Damien Wassom William Asah Che Glen Takang Beyang Craftsman Ndula Ndula-Nan Landry Fotsing Silatsa Jospin Walter Kom Timma marie-Paule Kengoum Djam Rosine Arckange Barakielle Bindzi Abah nicolas Niemenak Department of Biochemistry Faculty of Science University of Yaounde 1 Yaounde Cameroon Laboratory of Biochemistry and Vegetal Physiology of Higher Teachers Training College University of Yaounde 1 Yaounde Cameroon Laboratory of Phytoprotection and Valorisation of Genetic Resources of The Biotechnology Centre University of Yaounde 1 Yaounde Cameroon Department of Biological Science Higher Teachers Training College University of Yaounde 1 Yaounde Cameroon

Plantain banana is an important cash crop that serves as stable food for millions of people around the world and contributes to income generation. Indeed, they provide a major staple food crop for millions of people and play an important role in the social fabric of many rural communities. Plantain banana cultivation encounters major problem of seedlings unavailability that are essential for the creation of new plantations, as well as parasitic constraints. Mycosphaerella fijiensis is the main pathogen attack constraints of banana plant responsible of black Sigatoka disease, and viruses, which can severely reduce the photosynthetic leaf area, leading to banana production losses of more than 80% in plantations with soil fertility problems. The repeated use of synthetic input is the origin of contamination to the environment, different pollution sources of plants and human health, as well as resistance to some strains of pathogens and plant fertilization problems over time. Recent works carried out in nursery have shown that vivoplants of plantains treated with biostimulants based on natural products notably Tithonia diversifolia biopromote good growth and less susceptibility to M. fijiensis. Indeed, an increase in agromorphological characteristics, good accumulation of growth and defense biomarkers was also observed. In this context, Tithonia diversifolia is shown to be involved in the stimulatory effect mechanism of growth promotion and defensive reaction of plantain vivoplants against various pathogens and it is suggested to be acting as a vital stimulator. This article reviews the current state of knowledge on plantain banana cultivation constraints and on the potential of Tithonia diversifolia in relation with its different stimulatory effects on plantain vivoplants.

关键词： Tithonia diversifolia Plantain Banana Black Sigatoka Disease Growth Biopromotion Bioprotection Induced Resistance Biofungicidal Effect

Impact of nanocrystallinity segregation on the growth and morphology of nanocrystal superlattices

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Nano Research 2013年第8期6卷 611-618页

作者： Yanfen Wan Herve Portals nicolas Goubet Alain Mermet marie-Paule Pileni Université Pierre et marie Curie Paris 6 UMR 7070 LM2N 8P 52 4 place Jussieu 75005 Paris France Centre National de la Recherche Scientifique Unitd Mixte de Recherche 7070 LM2N 4 place Jussieu 75005 Paris France Université Lyon I Institut Lumiére Matiére Unité Mixte de Recherche 5306 CNRS Bāt. A. Kastler 10 rueAda Byron 69622 Villeurbanne France

A colloidal solution of 5 nm Au tetradecanethiol-coated nanoparticles is syn-thesized. After fast evaporation of one drop, ordered monolayers both composed of single domain and polycrystalline nanocrystals are obtained. On increasing the amount of materials and the evaporation time, nanocrystal films with irregular outlines are produced together with close-packed 3D superlattices exhibiting a truncated-tetrahedral shape. Using low-frequency micro-Raman scattering spectroscopy and electron microscopy the building block nanocrystallinity is characterized. Spontaneous nanocrystallinity segregation is revealed： the truncated-tetrahedral supracrystals are shown to mainly contain single domain building blocks while the supracrystalline films are composed of a mixture of single domain and polycrystalline nanocrystals. This observation points out the correlation between the nanocrystallinity segregation involved in the growth of the nanocrystal superlattices and their morphology.

关键词： supracrystals close-packed self-assembly metal nanoparticles Raman scatteringspectroscopy

Benchmarks of 3D Laplace Equation Solvers in a Cubic Configuration for Streamer Simulation

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Plasma Science and Technology 2016年第5期18卷 538-543页

作者： Joseph-marie PLEWA Olivier DUCASSE Philippe DESSANTE Carolyn JACOBS Olivier EICHWALD nicolas RENON Mohammed YOUSFI Universite de Toulouse UPS INPT LAPLACE (Laboratoire Plasma et Conversion d'Energie) Toulouse Cedex 9 F-31062 France Group of Electrical Engineering-Paris (GeePs) UMR 8507 CNRS CentraleSupelec Universite Paris-Sud-UPMC 11 rue Joliot-Curie Plateau de Moulon 91192 Gif-sur-Yvette Cedex F-91192 France CALMIP Universite de Toulouse-CNRS-INPT-INSA-UPS UMS 3667 France CNRS LAPLACE Toulouse F-31062 France

The aim of this paper is to test a developed SOR R＆B method using the Chebyshev accelerator algorithm to solve the Laplace equation in a cubic 3D configuration. Comparisons are made in terms of precision and computing time with other elliptic equation solvers proposed in the open source LIS library. The first results, obtained by using a single core on a HPC, show that the developed SOR R＆B method is efficient when the spectral radius needed for the Chebyshev acceleration is carefully pre-estimated. Preliminary results obtained with a parallelized code using the MPI library are also discussed when the calculation is distributed over one hundred cores.

关键词： numerical methods for elliptic equations high performance computing 3Dstreamer simulation SOR IDR BiCGSTAB

Synthesis of Branched Poly(butylene succinate):Structure Properties Relationship

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Chinese Journal of Polymer Science 2016年第7期34卷 873-888页

作者： marie Vandesteene nicolas Jacquel Rene Saint-Loup Nadège Boucard Christian Carrot Alain Rousseau Francoise Fenouillot Unive Lyon INSA-LyonIngenierie des Materiaux PolymeresIMPUMR5223F-69621VilleurbanneFrance Univ Lyon Universite Jean MonnetIngenierie des Materiaux PolymeresIMPUMR5223F-42023 Saint-EtienneFrance Roquette Freres.Polymer Chemistry Department F-62136 LestremFrance MBD Texinov F-38110 Saint-Didier-de-la-TourFrance

A series of branched poly（butylene succinate）（PBS） were synthesized with several branching agents namely trimethylol propane（TMP）, malic acid, trimesic acid, citric acid and glycerol propoxylate. The structure of the branched polymers was analyzed by SEC and ~1H-NMR. The effect of branching agent structure on crystallization was also investigated and played a significant role. Isothermal studies showed that glycerol propoxylate could act as a nucleating agent. By contrast high content of TMP disturbed the regularity of the chain and hindered the crystallization of PBS. From the non-isothermal kinetic study, it was found that glycerol propoxylate increased noticeably the crystallization rate due to the flexible structure of the branching agent. A secondary nucleation was observed with glycerol propoxylate attributed to the crystallization of amorphous fraction included between crystallites formed at the primary crystallization. Chain topology was obtained through rheological investigations and the synthesized polymers showed a typical behavior of a mixture of linear and randomly branched PBS. The incorporation of branches improved the processability of PBS for film blowing application and the modulus and the stress at break of the resulting film were significantly increased.

关键词： Poly(butylene succinate) Branching Crystallization Properties

A case of atypical neurological symptoms and high cerebro-spinal fluid acetone level due to transdermal exposure to isopropanol

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World Journal of Emergency Medicine 2023年第2期14卷 152-154页

作者： Côme Fruit Sophie Guillotin marie Wolfrum Thomas Lanot Florent Battefort Pierre Girardie marie Rafiq nicolas Delcourt Poison Control Center Toulouse University HospitalToulouse 31509France Neurology Department Toulouse University HospitalToulouse 31509France Pharmacokinetics and Toxicology Laboratory Federative Institute of BiologyToulouse University HospitalToulouse 31509France Poison Control Center and INSERM 1214 Toulouse University HospitalToulouse 31509France

Dear editor, Isopropanol, also named 2-propanol or isopropyl alcohol(IPA), is a colorless, volatile liquid found in numerous household chemicals, such as cleaners and disinfectants, which typically contain a 70% solut... 详细信息

关键词： acetone typically alcohol