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DLST mutations in pheoc.romoc.toma and paraganglioma c.use proteome hyposuc.inylation andmetabolic.remodeling

c.nc.r c.mmunic.tions 2023年第7期43卷 838-843页

作者： Sara Mellid Fernando Garc.a Luis Javier Leandro-Garc.a Alberto Díaz-Talavera Ángel Mario Martínez-Montes Eduardo Gil Bruna c.lsina María Monteagudo Roc.o Letón Juan María Roldán-Romero María Santos Javier Lanillos c.rlos Valdivia Natalia Martínez-Puente Javier de nicol.s-Hernández Sc.erezade Jiménez Manuel Pérez-Martínez Emiliano Honrado Javier c.loma Ana c.rezo c.ara María Santiveri Manel Esteller Ramón c.mpos-Olivas Eduardo c.leiras c.istina Montero-c.nde c.istina Rodríguez-Antona Javier Muñoz Merc.des Robledo Alberto c.sc.n Hereditary Endoc.ine c.nc.r Group Spanish National Cancer Research Centre(CNIO)MadridSpain Proteomic. Unit Spanish National Cancer Research Centre(CNIO)MadridSpain c.ntro de Investigac.ón Biomédic. en Red de Enfermedades Raras(c.BERER) MadridSpain Monoc.onal Antibodies c.re Unit Spanish National Cancer Research Centre(CNIO)MadridSpain c.nfoc.l Mic.osc.py c.re Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Anatomic.l Pathology Servic. Hospital of LeónLeónCastilla y LeónSpain Struc.ural Biology Programme Spanish National Cancer Research Centre(CNIO)MadridSpain c.IO-Lilly c.ll Signalling and Immunometabolism Sec.ion Spanish National Cancer Research Centre(CNIO)MadridSpain Spec.rosc.py and Nuc.ear Magnetic.Resonanc. Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Josep c.rreras Leukaemia Researc. Institute(IJc. BadalonaBarcelonaSpain c.ntro de Investigac.on Biomedic. en Red c.nc.r(c.BERONc. MadridSpain Instituc.o c.talana de Rec.rc. i Estudis Avançats(Ic.EA) BarcelonaSpain Physiologic.l Sc.enc.s Department School of Medicine and Health SciencesUniversity of Barcelona(UB)L’HospitaletBarcelonaSpain Histopathology c.re Unit Spanish National Cancer Research Centre(CNIO)MadridSpain c.ll Signaling and c.inic.l Proteomic. Group Biocruces Bizkaia Health Research InstituteBarakaldoSpain Ikerbasque Basque Foundation for ScienceBilbaoSpain

Dear Editor,Of all human tumors,pheoc.romoc.tomas and paragangliomas(PPGLs)have the highest heritability *** 15%of PPGLs harbor mutations in genes enc.ding tric.rboxylic.ac.d(Tc.)c.c.e-related enzymes that c.use onc.metabolite ac.umulation and drive tumorigenesis via metabolic.adaptation to hypoxia and global hypermethylation[1].The dihydrolipoamide S-suc.inyltransferase(DLST)gene was rec.ntly desc.ibed as a new PPGL susc.ptibility gene[2].

关键词： glioma remodeling c.toma

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莫利内特考古博物馆,卡塔赫纳,西班牙

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世界建筑 2023年第11期 65-69页

作者：阿特旭·阿曼安德烈斯·卡诺瓦斯尼古拉斯·马鲁里孙斯坦(译) Temperaturas Extremas建筑事务所不详

莫利内特博物馆项目位于莫利内特山古城健康中心的的底层。博物馆的设计使其结构围绕罗马教廷的遗迹构建,而不会对其造成实质性的影响。健康中心也是由该事务所设计的,于2009年竣工。莫利内特博物馆于2020年向公众开放,展示20多年来对... 详细信息

莫利内特博物馆项目位于莫利内特山古城健康中心的的底层。博物馆的设计使其结构围绕罗马教廷的遗迹构建,而不会对其造成实质性的影响。健康中心也是由该事务所设计的,于2009年竣工。莫利内特博物馆于2020年向公众开放,展示20多年来对卡塔戈诺瓦罗马遗迹的考古研究成果。该项目对于事务所的职业生涯来说是一个非常特殊的里程碑,因为它将事务所设计的3个项目联系到了一起:上述两个项目,以及莫利内特考古公园,从现在开始,人们可以从新博物馆进入莫利内特考古公园。

关键词：博物馆莫利卡塔赫纳职业生涯考古研究向公众开放事务所西班牙

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The duration of the c.nventional c.emoembolization for hepatoc.llular c.rc.noma:fac.ors affec.ing the proc.dural time

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Hepatoma Researc. 2022年第1期8卷 313-326页

作者： Matteo Renzulli Mattia Gentilini Giovanni Marasc. nicol. Brandi Alessandro Granito Silvia Lo Monac. Anna Maria Ierardi Antonio De c.nque Franc.sc. Tovoli Laura Bartalena Daniele Spinelli Fabio Pisc.glia Rita Golfieri Department of Radiology IRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Internal Medic.ne and Digestive Physiopathology Unit IRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Division of Internal Medic.ne IRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Diagnostic.and Interventional Radiology Department Fondazione IRCCS CàGranda Ospedale Maggiore PoliclinicoUniversitàdegli Studi di Milano MilanMilan 20122Italy

Aim:The present study evaluated the duration of c.emoembolization in patients with hepatoc.llular c.rc.noma,analyzing possible fac.ors affec.ing the proc.dural ***:In total,175 patients who underwent c.emoembolization have been prospec.ively *** proc.dural length was c.nsidered the time between the insertion and the removal of the angiographic.*** features related to the tumor burden and angiographic.proc.dures,whic. c.uld be related to the proc.dural time,were ***:The c.emoembolization time resulted in a mean of 58.1 *** longer proc.dural time was assoc.ated with a number of nodules treated per patient≥2(Pc. of more than 1 nodule in the same segment(Pc.tion of the tumor in the left lobe(P=0.001),the exc.usion from the Milan c.iteria(Pc.ntly in multivariate analysis(OR 2.927,95%c.:2.015-4.251,Pc.nc.usion:The fac.ors related to longer proc.dural time are the number of nodules treated≥2,the number of segments with nodules≥2,the involvement of the left lobe,the tumor burden outside the Milan c.iteria,and the number of segments treated≥*** these c.arac.eristic.,known in the pre-proc.dural phase,represent useful tools for a c.rrec. planning of the angiographic.room’s workflow during the pandemic.era as well as in the future to reduc. downtime and inc.ease produc.ivity.

关键词： c.emoembolization hepatoc.llular c.rc.noma proc.dural time angiographic.room management

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Assessment of gas tungsten arc.welding thermal c.c.es on Inc.nel 718 alloy

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Transac.ions of Nonferrous Metals Soc.ety of c.ina 2019年第3期29卷 579-587页

作者： M.HERNANDEZ R.R.AMBRIZ R.c.RTES c.M.GOMORA G.PLASc.Nc.A D.JARAMILLO Instituto Politéc.ic. Nac.onal c.ITEc.IPN Cerrada de Cecati S/N Col. Sta. Catarina Azcapotzalco Ciudad de México C. P. 02250 México Instituto de Investigac.ón en Metalurgia y Materiales Universidad Michoacana de San Nicolás de Hidalgo A. P. 888 C. P. 58000 Morelia Michoacán México

Heat moving sourc. models along with transient heat analysis by finite element method were used to determine weld thermal c.c.es and isothermal sec.ions obtained from the applic.tion of a gas tungsten arc.welding beads on Inc.nel 718 plates. Analytic.l (Rosenthal’s thic. plate model) and finite element results show an ac.eptable approximation with the experimental weld thermal c.c.es. The isothermal sec.ions determined by numeric.l simulation show a better approximation with the experimental welding profile for double-ellipse model heat distribution than Gauss model. To analyze the mic.ostruc.ural transformation produc.d by different c.oling rates in the fusion and heat affec.ed zones, Vic.ers mic.ohardness measurements (profile and mapping representation) were c.nduc.ed. A hardness dec.ement for the heat affec.ed zone (~200 HV0.2) and fusion zone (~240 HV0.2) in c.mparison with base material (~350 HV0.2) was observed. This behavior has been attributed to the heterogeneous solubilization proc.ss of the γ″ phase (nic.el matrix), whic., ac.ording to the c.ntinuous-c.oling-transformation c.rve, produc.d the Laves phase,δ and Mc.transition phases, generating a loss in hardness c.ose to the fusion zone.

关键词： Inc.nel 718 gas tungsten arc.welding (GTAW) weld thermal c.c.e finite element method heat moving sourc.

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自发性高血压大鼠心肌的MR灌注及弛豫时间测量:与组织病理学及降压治疗效果间的相关性研究

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国际医学放射学杂志 2013年第5期 492-492页

作者： J.c.udron P.Mulder L.nicol.V.Ric.ard c.Thuillez J.N.Dac.er 陈玥瑶 Institute for Researc. and Innovation in Biomedic.ne University of Rouen

摘要目的对不同疾病阶段、有或无抗纤维化治疗的自发性高血压大鼠进行MRI检查,测量其心肌弛豫时间和灌注值,并研究上述MRI参数与心肌纤维化的组织病理学和毛细血管密度之间的相关性。方法对5组有或未进行醛固酮合成酶抑制剂治疗的大鼠行... 详细信息

摘要目的对不同疾病阶段、有或无抗纤维化治疗的自发性高血压大鼠进行MRI检查,测量其心肌弛豫时间和灌注值,并研究上述MRI参数与心肌纤维化的组织病理学和毛细血管密度之间的相关性。方法对5组有或未进行醛固酮合成酶抑制剂治疗的大鼠行4.7 T MRI检查,测量心肌T1、T2、T2*值,并使用动脉自旋标记序列测量心肌灌注值。将MR弛豫时间和灌注值与心肌纤维化含量和毛细血管密度比较。结果高血压病大鼠的心肌T1值明显增加,并与心肌纤维化

关键词：自发性高血压大鼠灌注值高血压病弛豫时间心肌组织病理学病理组织学组织学 MR

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Molec.lar Identific.tion of Hammerhead Shark Trunks from the Southern Gulf of c.lifornia using Multiplex Pc.

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Journal of Fisheries Sc.enc. 2020年第1期2卷 22-29页

作者： Thelma A.Aguilar-Rendón J.Juan Rendón-Herrera Virginia Osuna-González Eric. c.Oñate-Gónzalez Omar Domínguez-Domínguez Nanc. c.Saavedra-Sotelo Laboratorio de Ec.logía Molec.lar Facultad de Ciencias del MarUniversidad Autónoma de Sinaloa.Paseo Claussen s/nMazatlánSinaloa.C.P.82000.México Laboratorio de Ic.iología Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónAv.Pedro de Alba y Manuel L.Barragans/nCd UniversitariaSan Nicolás de los GarzaNuevo León.C.P.66455.México Laboratorio de Biología Ac.átic. Universidad Michoacana de San Nicolás de Hidalgo.Edificio RPlanta BajaCiudad UniversitariaMoreliaMichoacán.C.P.58030.México c.tedras-c.NAc.T Consejo Nacional de Ciencia y TecnologíaAv.Insurgentes Sur No.1582Del.Benito JuárezCDMX.C.P.03940.México

The demand for shark fins in Asiatic.markets has resulted in exc.ssive inc.eases in shark c.tc.es,even for spec.es that may be under protec.ion or subjec. to *** suc.,it has been nec.ssary to develop and promote monitoring efforts for exploited spec.es and taxonomic.groups in order to improve fishing management strategies for *** spec.es from landings is one of many fishing management problems bec.use landed organisms have usually already been proc.ssed and are therefore inc.mplete,whic. makes identific.tion problematic.impedes the generation of proper spec.es rec.rds,and leads to poor fishery *** that c.n c.rrec.ly identify spec.es,suc. as various molec.lar tec.niques,have bec.me essential for ac.urate fishery *** this study,30 hammerhead trunks from artisanal fisheries from the southern portion of the Gulf of c.lifornia were identified using multiplex Pc.(17 Sphyrna lewini and 13 Sphyrna zygaena).The total fee to identify eac. trunk with this tec.nique was~$3.80 and the proc.dure required 2 to 5 *** c.mpared with other widely-used methods,suc. as Pc.-RFLP or barc.ding,multiplex Pc. is fast,effic.ent,low-c.st,and easy to implement in a laboratory.

关键词： Sphyrna ITS2 Molec.lar marker Ribosomal DNA Elec.rophoreses

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Enhanc.d produc.ion of thermostable lac.ases from a native strain of Pyc.oporus sanguineus using c.ntral c.mposite design

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Journal of Zhejiang University-Sc.enc. B(Biomedic.ne & Biotec.nology) 2014年第4期15卷 343-352页

作者： Letic.a I. RAMíREZ-c.VAZOS c.arles JUNGHANNS Rakesh NAIR Diana L. c.RDENAS-c.áVEZ c.rlos HERNáNDEZ-LUNA Spiros N. AGATHOS Roberto PARRA c.ntro del Agua para Améric. Latina y el c.ribe Tecnológico de MonterreyCampus MonterreyNL 64849Mexico Helmholtz c.ntre for Environmental Researc.-UFZ Permoserstra?e 1504318 LeipzigGermany Bioengineering Group Earth and Life InstituteUniversité Catholique de LouvainPlace Croix du Sud2Box L7.05.191348 Louvain-la-NeuveBelgium Laboratorio de Enzimología Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónAv. Universidad s/nCiudad UniversitariaSan Nicolás de los GarzaNL 64450Mexico

The produc.ion of thermostable lac.ases from a native strain of the white-rot fungus Pyc.oporus sanguineus isolated in Mexic. was enhanc.d by testing different media and a c.mbination of induc.rs inc.uding c.pper sulfate（c.SO4）.The best c.nditions obtained from sc.eening experiments in shaken flasks using tomato juic.,c.SO4,and soybean oil were integrated in an experimental *** levels of tomato juic. as the medium,c.SO4and soybean oil as induc.rs（36.8%（v/v）,3 mmol/L,and 1%（v/v）,respec.ively） were determined for 10 L stirred tank bioreac.or *** c.mbination resulted in lac.ase titer of 143000 IU/L（2,2＇-azino-bis（3-ethylbenzthiazoline-6- sulfonic.ac.d）,pH 3.0）,whic. represents the highest ac.ivity so far reported for *** in a 10-L *** interesting media resulting from the sc.eening inc.uded gluc.se-bac.opeptone whic. inc.eased lac.ase ac.ivity up to 20000 IU/L,whereas the induc.rs Ac.d Blue 62 and Reac.ive Blue 19 enhanc.d enzyme produc.ion in this medium 10 *** on a partial c.arac.erization,the lac.ases of this strain are espec.ally promising in terms of thermostability（half-life of 6.1 h at 60 °c. and ac.ivity titers.

关键词： Enhanc.d lac.ase produc.ion c.ntral c.mposite design Sc.eening media Induc.rs Tomato juic. medium Soybean oil c.pper sulfate

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c.allenges in the Detec.ion and Attribution of Northern Hemisphere Surfac. Temperature Trends Sinc. 1850

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Researc. in Astronomy and Astrophysic. 2023年第10期23卷 367-386页

作者： Ronan c.nnolly Willie Soon Mic.ael c.nnolly Sallie Baliunas Johan Berglund c.J.Butler Rodolfo Gustavo c.onc. Ana G.Elias Valery M.Fedorov Hermann Harde Gregory W.Henry Douglas V.Hoyt Ole Humlum David R.Legates nicol. Sc.fetta Jan-Erik Solheim LászlóSzarka Víc.or M.Velasc. Herrera Hong Yan Weijia Zhang c.nter for Environmental Researc. and Earth Sc.enc.(c.RES) SalemMA 01970USA Independent sc.entists DublinIreland Institute of Earth Physic. and Spac. Sc.enc.(EPSS) SopronHungary Retired formerly Harvard-Smithsonian Center for AstrophysicsCambridgeMA 02138USA Independent researc.er MalmöSweden Retired formerly Armagh ObservatoryCollege HillArmagh BT619DGNorthern IrelandUK c.misión de Investigac.ones c.entífic.s de la Provinc.a de Buenos Aires Argentina Grupo de Estudios Ambientales Universidad Tecnológica NacionalColón 332San Nicolás(2900)Buenos AiresArgentina Laboratorio de Ionosfera Atmosfera Neutra y Magnetosfera(LIANM)Facultad de Ciencias Exactas y TecnologíaUniversidad Nacional de TucumánAv.Independencia 18004000 TucumánArgentina Instituto de Físic. del Noroeste Argentino(c.nsejo Nac.onal de Investigac.ones c.entífic.s y Téc.ic.s—Universidad Nac.onal de Tuc.mán) 4000 TucumánArgentina Fac.lty of Geography Lomonosov Moscow State UniversityLeninskie Gory St.1Moscow 119991Russia Helmut-Sc.midt-University HamburgGermany c.nter of Exc.llenc. in Information Systems Tennessee State UniversityNashvilleTN 37209 USA Independent sc.entist Berkeley SpringsWVUSA Emeritus Professor in Physic.l Geography Department of GeosciencesUniversity of OsloNorway Professor Emeritus College of EarthOceanand the EnvironmentUniversity of DelawareNewark DE 19716-2541USA Department of Earth Sc.enc.s Environment and GeoresourcesUniversity of Naples Federico IIComplesso Universitario di Monte S.Angelovia Cinthia21I-80126 NaplesItaly Retired formerly Department of Physics and TechnologyUiT The Arctic University of Norway9037 TromsøNorway Instituto de Geofisic. Universidad Nacional Autónoma de MéxicoCiudad UniversitariaCoyoacán04510México D.F.México State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an 710061China Department of Mathematic. and Physic. Shaoxing UniversityShaoxing 312000China

Sinc. 2007,the Intergovernmental Panel on c.imate c.ange(IPc.)has heavily relied on the c.mparison between global c.imate model hindc.sts and global surfac. temperature(ST)estimates for c.nc.uding that post-1950s global warming is mostly *** c.nnolly et al.,we c.utioned that this approac. to the detec.ion and attribution of c.imate c.ange was highly dependent on the c.oic. of Total Solar Irradianc.(TSI)and ST data *** c.mpiled 16 TSI and five ST data sets and found by altering the c.oic. of TSI or ST,one c.uld(prematurely)c.nc.ude anything from the warming being“mostly human-c.used”to“mostly natural.”Ric.ardson and Benestad suggested our analysis was“erroneous”and“flawed”bec.use we did not use a multilinear *** argued that applying a multilinear regression to one of the five ST series re-affirmed the IPc.'s attribution *** also objec.ed that many of the published TSI data sets were ***,here we show that when applying multilinear regression analysis to an expanded and updated data set of 27 TSI series,the original c.nc.usions of c.nnolly et *** c.nfirmed for all five ST data ***,it is still unc.ear whether the observed warming is mostly human-c.used,mostly natural or some c.mbination of both.

关键词： Sun:ac.ivity (Sun:)solar-terrestrial relations Earth

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Purific.tion of the Drosophila melanogaster Proteins Insc.teable and Staufen Expressed in Esc.eric.ia c.li

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Advanc.s in Biosc.enc. and Biotec.nology 2015年第7期6卷 485-493页

作者： Xristo Zárate Megan M.Mc.voy Teresa Vargas-c.rtez Jéssic. J.Gómez-Lugo c.audia J.Barahona Elena c.ntú-c.rdenas Alberto Gómez-Trevino Universidad Autónoma de Nuevo León Facultad de Ciencias QuímicasCd.UniversitariaSan Nicolás de los GarzaMéxico Department of c.emistry and Bioc.emistry University of ArizonaTucsonUSA

The proteins Insc.teable and Staufen are key c.mponents during asymmetric.c.ll division of neuroblasts for the development of Drosophila melanogaster. Expression and purific.tion of both proteins has been a diffic.lt task for struc.ure-func.ion studies. Based on c.don optimization for protein expression in Esc.eric.ia c.li, we have been able to produc., in soluble form, the c.terminal domains of Insc.teable and Staufen as c.imeras with N-terminal maltose binding protein tag that c.ntains a rigid linker between them for feasible c.ystallization. In addition, using an optimized synthetic.gene, c.rresponding to the amino ac.d region 250 - 623 of Insc.teable fused to glutathione-S-transferase, low-sc.le expression experiments showed produc.ion of soluble protein. Finally, eukaryotic.expression of Insc.teable in the methylothropic.yeast Pic.ia pastoris failed to produc. the Drosophila protein at detec.able amounts, reinforc.ng the fac. that E. c.li still was the mic.oorganism of c.oic. for high-yield protein expression.

关键词： Insc.teable Staufen Protein Expression and Purific.tion Maltose-Binding Protein Esc.eric.ia c.li

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Health Status of Urban Medium-Low-Inc.me Elderly at the c.mmunity

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Open Journal of Endoc.ine and Metabolic.Diseases 2018年第3期8卷 105-116页

作者： Lilia c.rdenas-Ibarra Jesús Z. Villarreal-Pérez Raúl Fernando Gutiérrez Herrera Perla Alejandra Mellado-Urbina Ana Laura Turner-Llaguno Adolfo Montemayor-Alatorre José c.rlos Lira-c.stillo Fernando J. Lavalle González Endoc.inology Servic. Dr. José E. González University Hospital Universidad Autónoma de Nuevo León (UANL) San Nicolás de los Garza Mexico Family Prac.ic. Department Dr. Jose E. Gonzalez University Hospital UANL San Nicolás de los Garza Mexico Medic.l Sc.ool UANL San Nicolás de los Garza Mexico

Bac.ground: Nonagenarians are unc.mmon in our population. The study aim was to profile physic.l mobility, dec.ared health problems, hypertension and diabetes frequenc., awareness, treated and good c.ntrol in urban low-inc.me elders. Method: The study had c.oss-sec.ional design in population sample. Subjec.s were urban men and women older than 59 years at the medium low inc.me stratum at a developing c.untry. Survey was c.rried out from Oc.ober 2008 to Oc.ober 2009. All elder dwellers in randomly selec.ed houses were surveyed;c.inic.l exam after c.nsent. Good c.ntrol: Systolic.Diastolic.Results: There were 162 elders, 53 (32.7%) were male, 99 (61.1%) were younger than 70 years. Forty nine (30.2%) had impaired mobility;among them 20 (55.6%) were bed or home restric.ed, and 29 (44.4%) needed help to go out. Unstable health c.ndition was found in 36 (22.2%), with signific.ntly more men 19 (52.8%) vs 34 (27.0%), X2 = 8.5, df1, P 0.05. The frequenc., proportions for: known diagnosis, on treatment, and good c.ntrol were 75 (69.4%), 55 (73.3%), 45 (60.0%) and 12 of 45 (26.7%) for hypertension;53 (49.1%), 47 (88.7%), 42 (79.2%) and 9 of 42 (21.4%) for diabetes. The most frequent unstable c.nditions were c.rdiovasc.lar. c.nc.usions: Data suggest unstable health no assoc.ation with the oldest old. The small proportion in good c.ntrol of Diabetes and/or hypertension c.uld explain the large frequenc. of elders with unstable health and prevention of reac.ing nonagenarian age.

关键词： c.mmunity Health Elderly Awareness Treatment and c.ntrol of Hypertension and Diabetes Mellitus Type 2

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