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Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

Journal of Animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi ribas-Maynou Isabel Barranco Camila peres rubio Sergi Bonet Jordi roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for research and Advanced Studies(ICrEA) S08010 BarcelonaSpain

Background protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SrF)and post sperm-rich(pSrF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(Sp),with differences being also observed within the SrF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the SrF(SrF-p1),the remaining portion of the sperm-rich fraction(SrF-p2),and the post sperm-rich fraction(pSrF).While chromatin protamination was found to be similar between the different ejaculate fractions(p>0.05),chromatin condensation was seen to be greater in SrF-p1 and SrF-p2 than in the pSrF(p=0.018 and p=0.004,respectively).regarding DNA integrity,no differences between fractions were observed(p>0.05).As the SrF-p1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in Sp,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SrF-p1 than in SrF-p2 and pSrF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;prved to be correlated to the sperm concentration of each fraction(rs=0.973;pperm DNA integrity was not found to differ between ejaculate fractions,SrF-p1 and SrF-p2 were observed to exhibit greater chromatin condensation than the *** could be related to the OSi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions pig protamination Sperm

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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero Estel Vinolas‑Vergés Sergi Bonet Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Institution for research and Advanced Studies(ICrEA) Barcelona ES‑08010Spain

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qpCr method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently *** First, the q pCr method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater *** These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

关键词： Fertility Mitochondria Motility mtDNA copy number qpCr Sperm

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primary carbohydrate metabolism genes participate in heat-stress memory at the shoot apical meristem of Arabidopsis thaliana

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Molecular plant 2024年第4期17卷 676-676页

作者： Justyna Jadwiga olas Federico Apelt Maria Grazia Annunziata Sheeba John Sarah Isabel richard Saurabh Gupta Friedrich Kragler Salma Balazadeh Bernd Mueller-roeber 不详

(Molecular plant 14,1508-1524,September 62021)After the original publication of the above article,attentive readers identified errors in Figure 4H that needed correction as *** 4H contained the erroneous labels“10 DAp(7 DAT),”which should have each read,“7 DAp(4 DAT).”To avoid any confusion and ensure consistency across our figures,we modified some of the Col-0 images in a corrected version of Figure 4H below to resemble the exact same Col-0 images as in Figure *** details of the changes are as follows:rotation(Col-0 primed,all timepoints;Col-0 Triggered,3 DAp and 5 DAp;Col-0 primed/triggered,3 DAp and 5 DAp),replacement(Col-0 Triggered,7 DAp),and different cropping(Col-0 primed/triggered,7 DAp).

关键词： Figure metabolism prime

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Effect of drug therapies on self-reported chemosensory outcomes after COVID-19

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 88-96页

作者： Marco A.Fornazieri Bruno M.Cunha Samuel p.nicácio Lucas K.Anzolin JoséL.B.da Silva Aristides Fernandes Neto Deusdedit Brandão Neto richard L.Voegels Fábio D.r.pinna Department of Surgery Londrina State UniversityLondrinaBrazil Department of Medicine Pontifical Catholic University of ParanáLondrinaBrazil Department of Otorhinolaryngology University of São PauloSao PauloBrazil Department of Otorhinolaryngology:Head and Neck Surgery Perelman School of MedicineSmell and Taste CenterUniversity of PennsylvaniaPhiladelphiaPennsylvaniaUSA

Objective:The aim of this study was to assess the relative efficacy of medications used following severe acute respiratory syndrome coronavirus-2(SArS-CoV-2)infection on self-reported alterations in taste and/or smell ***:Seven hundred and fourteen persons with self-reported postcoronavirus disease 2019(post-COVID-19)chemosensory disorders were personally interviewed regarding specific medications they were administered following the acute phase of the *** dependent measure—self-reported total recovery of chemosensory symptoms—was subjected to stepwise logistic *** predictors included demographic and clinical variables,in addition to specific medications used to mitigate disease symptoms(i.e.,systemic corticosteroids,oseltamivir,vitamin C,ibuprofen,hydroxychloroquine,azithromycin,ivermectin,nitazoxanide,anticoagulants,and zinc).results:The median time between COVID-19 symptom onset and the interviews was 81 days(interquartile range:60-104).Of the 714 subjects,249(34.9%)reported total recovery of their chemosensory function;437(61.2%)had at least one treatment since the beginning of the *** and those with more comorbidities had undergone more *** recovery rates of the treated and nontreated groups did not differ ***,respondents who had used nitazoxanide tended to have a higher rate of self-reported taste or smell *** who took oral zinc were less likely to ***:No medication employed during the first months after SArS-CoV-2 infection had a clear positive effect on returning self-reported smell or taste function to normal,although nitrazoxide trended in a positive *** zinc had a negative effect on the reported recovery of these senses.

关键词： chemosensory disorder coronavirus infections COVID-19 drug therapy SArS-CoV-2 smell loss taste loss

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学校的手机管理令人忧心中快

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商业周刊（中文版） 2024年第15期 20-21页

作者： nic Querolo 王非(译) 不详

在美国,超过三分之二的孩子在13岁生日前就拥有了一部智能手机,而智能手机的普及让家长、教育工作者和地方官员对学校的手机管理忧心仲。

关键词：教育工作者智能手机管理

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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs.

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畜牧与生物技术杂志(英文版) 2024年第4期 1550页

作者： Marc Llavanera Yentel Mateo-Otero Estel Vi?olas-Vergés Sergi Bonet Marc Yeste

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Lessons from a Marine Spatial planning data management process for Ireland

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International Journal of Digital Earth 2021年第2期14卷 139-157页

作者： Sarah Flynn Will Meaney Adam M.Leadbetter Jeffrey p.Fisher Caitriona nic Aonghusa Marine Institute RinvilleOranmoreCounty GalwayH91 R673Ireland

This paper presents a framework containing ten components to deliver a data management process for the storage and management of data used for Marine Spatial planning(MSp)in *** work includes a data process flow and a recommended solution *** architecture includes a central data catalogue and a spatial storage *** components of the process are presented to maximise the reuse potential of any dataset within an MSp *** terms‘Suitability’and‘readiness’in the MSp context are offered as both formal and considered assessments of data,as is the applicability of a data stewardship maturity *** data contained in such a storage system can be published externally to potential consumers of these data is also *** process presents a means of managing data and metadata to ensure data lineage is optimised by carrying information about the origin of and the processing applied to the data;to evaluate the quality and relevance of geospatial datasets for use in MSp decisions in *** process was piloted in the National Marine planning Framework for Ireland in the development of draft map products;feedback from the public consultation is ongoing and not presented.

关键词： Marine Spatial planning MSp marine data storage&management data governance reuse&integration

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Micro-computed tomography utility for estimation of intraparenchymal spinal cord cystic lesions in small animals

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Neural regeneration research 2021年第11期16卷 2293-2298页

作者： Mahmoud Farrag Dipak D.pukale nic D.Leipzig Integrated Bioscience program The University of AkronAkronOHUSA Department of Chemical Biomolecularand Corrosion EngineeringThe University of AkronAkronOHUSA Department of Chemical Biomolecularand Corrosion EngineeringIntegrated Bioscience ProgramThe University of AkronAkronOHUSA

precise assessment of spinal cord cystic lesions is crucial to formulate effective therapeutic strategies,yet histological assessment of the lesion remains the primary method despite numerous studies showing inconsistent results regarding estimation of lesion size via *** the other hand,despite numerous advances in micro-computed tomography(micro-CT)imaging and analysis that have allowed precise measurements of lesion size,there is not enough published data on its application to estimate intraspinal lesion size in laboratory animal *** work attempts to show that micro-CT can be valuable for spinal cord injury research by demonstrating accurate estimation of syrinx size and compares between micro-CT and traditional histological *** used a post-traumatic syringomyelia rat model to compare micro-CT analysis to conventional histological *** study showed that micro-CT can detect lesions within the spinal cord very similar to ***,micro-CT appears to provide more accurate estimates of the lesions with more measures(e.g.,surface area),can detect compounds within the cord,and can be done with the tissue of interest(spinal cord)*** summary,the experimental work presented here provides one of the first investigations of the use of micro-CT for estimating the size of intraparenchymal cysts and detecting materials within the spinal *** animal procedures were approved by the University of Akron Institutional Animal Care and Use Committee(IACUC)(protocol#LrE 16-05-09 approved on May 14,2016).

关键词： cyst size histology intraparenchymal cysts lesion size estimate micro-CT post-traumatic syringomyelia spinal cord imaging spinal cord injury spinal cystic lesion syrinx

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Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm‑rich fraction

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畜牧与生物技术杂志(英文版) 2024年第1期

作者： Estel Viñolas-Vergés Jordi ribas-Maynou Isabel Barranco Camila peres rubio Sergi Bonet Jordi roca Marc Yeste

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primary carbohydrate metabolism genes participate in heat-stress memory at the shoot apical meristem of Arabidopsis thaliana

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Molecular plant 2021年第9期14卷 1508-1524页

作者： Justyna Jadwiga olas Federico Apelt Maria Grazia Annunziata Sheeba John Sarah Isabel richard Saurabh Gupta Friedrich Kragler Salma Balazadeh Bernd Mueller-roeber University of potsdam Institute of Biochemistry and BiologyKarl-Liebknecht-Straße 24-25Haus 2014476 PotsdamGermany Max planck Institute of Molecular plant physiology Am Muehlenberg 114476 PotsdamGermany present address:Leiden University PO Box 95002300 RA Leidenthe Netherlands

In plants, the shoot apical meristem (SAM) is essential for the growth of aboveground organs. However, little is known about its molecular responses to abiotic stresses. Here, we show that the SAM of Arabidopsis thaliana displays an autonomous heat-stress (HS) memory of a previous non-lethal HS, allowing the SAM to regain growth after exposure to an otherwise lethal HS several days later. Using rNA sequencing, we identified genes participating in establishing the SAM's HS transcriptional memory, including the stem cell (SC) regulators CLAVATA1 (CLV1) and CLV3, HEAT SHOCK prOTEIN 17.6A (HSp17.6A), and the primary carbohydrate metabolism gene FrUCTOSE-BISpHOSpHATE ALDolasE 6 (FBA6). We demonstrate that sugar availability is essential for survival of plants at high temperature. HEAT SHOCK TrANSCrIpTION FACTOr A2 (HSFA2A) directly regulates the expression of HSp17.6A and FBA6 by binding to the heat-shock elements in their promoters, indicating that HSFA2 is required for transcriptional activation of SAM memory genes. Collectively, these findings indicate that plants have evolved a sophisticated protection mechanism to maintain SCs and, hence, their capacity to re-initiate shoot growth after stress release.

关键词： aldolase carbon metabolism heat stress shoot apical meristem thermomemory thermopriming

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