检索结果-南通市图书馆

Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

Journal of Animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi Ribas-Maynou Isabel Barranco Camila Peres Rubio Sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P>0.05),chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;P<0.01);this index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;P<0.001).Conclusion While sperm DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the *** could be related to the OSi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions Pig Protamination Sperm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

引用

Journal of Animal Science and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero Estel Vinolas‑Vergés Sergi Bonet Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Institution for Research and Advanced Studies(ICREA) Barcelona ES‑08010Spain

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently *** First, the q PCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater *** These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

关键词： Fertility Mitochondria Motility mtDNA copy number qPCR Sperm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Primary carbohydrate metabolism genes participate in heat-stress memory at the shoot apical meristem of Arabidopsis thaliana

引用

Molecular Plant 2024年第4期17卷 676-676页

作者： Justyna Jadwiga olas Federico Apelt Maria Grazia Annunziata Sheeba John Sarah Isabel Richard Saurabh Gupta Friedrich Kragler Salma Balazadeh Bernd Mueller-Roeber 不详

(Molecular Plant 14,1508-1524,September 62021)After the original publication of the above article,attentive readers identified errors in Figure 4H that needed correction as *** 4H contained the erroneous labels“10 DAP(7 DAT),”which should have each read,“7 DAP(4 DAT).”To avoid any confusion and ensure consistency across our figures,we modified some of the Col-0 images in a corrected version of Figure 4H below to resemble the exact same Col-0 images as in Figure *** details of the changes are as follows:rotation(Col-0 Primed,all timepoints;Col-0 Triggered,3 DAP and 5 DAP;Col-0 Primed/triggered,3 DAP and 5 DAP),replacement(Col-0 Triggered,7 DAP),and different cropping(Col-0 Primed/triggered,7 DAP).

关键词： Figure metabolism Prime

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of drug therapies on self-reported chemosensory outcomes after COVID-19

引用

World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 88-96页

作者： Marco A.Fornazieri Bruno M.Cunha Samuel P.nicácio Lucas K.Anzolin JoséL.B.da Silva Aristides Fernandes Neto Deusdedit Brandão Neto Richard L.Voegels Fábio D.R.Pinna Department of Surgery Londrina State UniversityLondrinaBrazil Department of Medicine Pontifical Catholic University of ParanáLondrinaBrazil Department of Otorhinolaryngology University of São PauloSao PauloBrazil Department of Otorhinolaryngology:Head and Neck Surgery Perelman School of MedicineSmell and Taste CenterUniversity of PennsylvaniaPhiladelphiaPennsylvaniaUSA

Objective:The aim of this study was to assess the relative efficacy of medications used following severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)infection on self-reported alterations in taste and/or smell ***:Seven hundred and fourteen persons with self-reported postcoronavirus disease 2019(post-COVID-19)chemosensory disorders were personally interviewed regarding specific medications they were administered following the acute phase of the *** dependent measure—self-reported total recovery of chemosensory symptoms—was subjected to stepwise logistic *** predictors included demographic and clinical variables,in addition to specific medications used to mitigate disease symptoms(i.e.,systemic corticosteroids,oseltamivir,vitamin C,ibuprofen,hydroxychloroquine,azithromycin,ivermectin,nitazoxanide,anticoagulants,and zinc).Results:The median time between COVID-19 symptom onset and the interviews was 81 days(interquartile range:60-104).Of the 714 subjects,249(34.9%)reported total recovery of their chemosensory function;437(61.2%)had at least one treatment since the beginning of the *** and those with more comorbidities had undergone more *** recovery rates of the treated and nontreated groups did not differ ***,respondents who had used nitazoxanide tended to have a higher rate of self-reported taste or smell *** who took oral zinc were less likely to ***:No medication employed during the first months after SARS-CoV-2 infection had a clear positive effect on returning self-reported smell or taste function to normal,although nitrazoxide trended in a positive *** zinc had a negative effect on the reported recovery of these senses.

关键词： chemosensory disorder coronavirus infections COVID-19 drug therapy SARS-CoV-2 smell loss taste loss

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Manipulation of polarization topology using a Fabry-Pérot fiber cavity with a higher-order mode optical nanofiber

引用

Photonics Research 2023年第6期11卷 1029-1037页

作者： MAKI MAEDA JAMEESH KELOTH SÍLE nic CHORMAIC Okinawa Institute of Science and Technology Graduate University OnnaOkinawa 904-0495Japan

Optical nanofiber cavity research has mainly focused on the fundamental ***,a Fabry-Pérot fiber cavity with an optical nanofiber supporting the higher-order modes(TE01,TM01,HEo21,and HEe21)is *** cavity spectroscopy,with mode imaging and analysis,we observed cavity resonances that exhibited complex,inhomogeneous states of polarization with topological features containing Stokes singularities such as C-points,Poincarévortices,and *** situ tuning of the intracavity birefringence enabled the desired profile and polarization of the cavity mode to be *** believe these findings open new research possibilities for cold atom manipulation and multimode cavity quantum electrodynamics using the evanescent fields of higher-order mode optical nanofibers.

关键词： polarization fiber optical

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Structural characterization of thin-walled microbubble cavities

引用

光子学研究:英文版 2023年第8期11卷 I0021-I0027页

作者： MOHAMMED ZIA JALALUDEEN SHILONG LI KE TIAN TOSHIO SASAKI SÍLE nic CHORMAIC Light-Matter Interactions for Quantum Technologies Unit Okinawa Institute of Science and Technology Graduate UniversityOnnaOkinawa 904-0495Japan College of Physics and Optoelectronic Engineering Harbin Engineering UniversityHarbin 150001China Scientific Imaging Section Okinawa Institute of Science and Technology Graduate UniversityOnnaOkinawa 904-0495Japan

Whispering gallery mode(WGM)microbubble cavities are a versatile optofluidic sensing platform owing to their hollow core *** increase the light–matter interaction and,thereby,achieve higher sensitivity,thinwalled microbubbles are ***,a lack of knowledge about the precise geometry of hollow microbubbles prevents us from having an accurate theoretical model to describe the WGMs and their response to external *** this work,we provide a complete characterization of the wall structure of a microbubble and propose a theoretical model for the WGMs in this thin-walled microcavity based on the optical waveguide *** characterization of the wavelength-scale wall is enabled by focused ion beam milling and scanning electron microscopy *** proposed theoretical model is verified by finite element method *** approach can readily be extended to other low-dimensional micro-/nanophotonic structures.

关键词： waveguide characterization bubble

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Blue band nonlinear optics and photodarkening in silica microdevices

引用

Photonics Research 2022年第9期10卷 2073-2080页

作者： KE TIAN JIBO YU FUCHUAN LEI JONATHAN WARD ANGZHEN LI PENGFEI WANG SÍLE nic CHORMAIC Okinawa Institute of Science and Technology Graduate University OnnaOkinawa 904-0495Japan Key Laboratory of In-Fiber Integrated Optics of Ministry of Education College of Physics and Optoelectronic EngineeringHarbin Engineering UniversityHarbin 150001China Department of Microtechnology and Nanoscience Chalmers University of TechnologySE-41296 GothenburgSweden Physics Department University College CorkCorkIreland Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

There are extensive studies to date on optical nonlinearities in microcavities at the near and mid-IR *** this research into the visible region is equally ***,we demonstrate a directly pumped,blue band Kerr frequency comb and stimulated Raman scattering(SRS)at 462 nm in a silica nanofiber-coupled whispering gallery microcavity ***,due to the high optical intensities achieved,photodarkening is unavoidable and can quickly degrade the optical quality of both the coupling optical nanofiber and the microcavity,even at very low pump ***,stable hyperparametric oscillation and SRS are demonstrated in the presence of photodarkening by taking advantage of in-situ thermal *** work highlights the challenges of silica-based,short wavelength nonlinear optics in high-quality,small mode volume devices and gives an effective method to overcome this apparent limitation,thus providing a baseline for optics research in the blue region for any optical devices fabricated from silica.

关键词： pumped pump scattering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Emission spectroscopy of NaYF_(4):Eu nanorods optically trapped by Fresnel lens fibers

引用

Photonics Research 2022年第2期10卷 332-339页

作者： Aashutosh Kumar Asa Asadollahbaik Jeongmo Kim Khalid Lahlil Simon Thiele Alois M.Herkommer Síle nic Chormaic Jongwook Kim Thierry Gacoin Harald Giessen Jochen Flck Universite Grenoble Alpes CNRSInstitut Neel38000 GrenobleFrance 4th Physics Institute and Research Center SCoPE University of Stuttgart70569 StuttgartGermany Universite Paris Saclay CNRSLaboratoire de Physique de la Matiere CondenseeEcole Polytechnique91128 PalaiseauFrance Institute of Applied Optics and Research Center SCoPE University of Stuttgart70569 StuttgartGermany Okinawa Institute of Science and Technology Graduate University Okinawa 904-0495Japan

NaYF_(4):Eu nanorods with high aspect ratios are elaborated and optically trapped using dual fiber optical tweezers in a counterpropagating geometry. High trapping efficiency is observed using converging beams, emitted from diffractive Fresnel lenses directly 3D printed onto cleaved fiber facets. Stable nanorod trapping and alignment are reported for a fiber-to-fiber distance of 200 μm and light powers down to 10 m W. Trapping of nanorod clusters containing one to three nanorods and the coupling of nanorod motion in both axial and transverse directions are considered and discussed. The europium emission is studied by polarization-resolved spectroscopy with particular emphasis on the magnetic and electric dipole transitions. The respective σ and π orientations of the different emission lines are determined. The angles with respect to the nanorod axes of the corresponding magnetic and electric dipoles are calculated. Mono-exponential emission decay with decay time of 4–5 ms is reported. It is shown that the nanorod orientation can be determined by purely spectroscopic means.

关键词： nanorods Fresnel trapping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Micro-computed tomography utility for estimation of intraparenchymal spinal cord cystic lesions in small animals

引用

Neural Regeneration Research 2021年第11期16卷 2293-2298页

作者： Mahmoud Farrag Dipak D.Pukale nic D.Leipzig Integrated Bioscience Program The University of AkronAkronOHUSA Department of Chemical Biomolecularand Corrosion EngineeringThe University of AkronAkronOHUSA Department of Chemical Biomolecularand Corrosion EngineeringIntegrated Bioscience ProgramThe University of AkronAkronOHUSA

Precise assessment of spinal cord cystic lesions is crucial to formulate effective therapeutic strategies,yet histological assessment of the lesion remains the primary method despite numerous studies showing inconsistent results regarding estimation of lesion size via *** the other hand,despite numerous advances in micro-computed tomography(micro-CT)imaging and analysis that have allowed precise measurements of lesion size,there is not enough published data on its application to estimate intraspinal lesion size in laboratory animal *** work attempts to show that micro-CT can be valuable for spinal cord injury research by demonstrating accurate estimation of syrinx size and compares between micro-CT and traditional histological *** used a post-traumatic syringomyelia rat model to compare micro-CT analysis to conventional histological *** study showed that micro-CT can detect lesions within the spinal cord very similar to ***,micro-CT appears to provide more accurate estimates of the lesions with more measures(e.g.,surface area),can detect compounds within the cord,and can be done with the tissue of interest(spinal cord)*** summary,the experimental work presented here provides one of the first investigations of the use of micro-CT for estimating the size of intraparenchymal cysts and detecting materials within the spinal *** animal procedures were approved by the University of Akron Institutional Animal Care and Use Committee(IACUC)(protocol#LRE 16-05-09 approved on May 14,2016).

关键词： cyst size histology intraparenchymal cysts lesion size estimate micro-CT post-traumatic syringomyelia spinal cord imaging spinal cord injury spinal cystic lesion syrinx

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Red list assessments of Chinese higher plants

引用

International Journal of Digital Earth 2023年第1期16卷 2762-2775页

作者： Liping Li Haining Qin Eimear nic Lughadha Yaomin Zheng Huawei Wan Jack Plummer Melanie-Jayne R.Howes Huiyuan Liu Yangming Jiang Tuo Wang Huihui Zhao Zhanfeng Shen Huiping Huang Chinese Academy of Sciences Aerospace Information Research InstituteBeijingPeople’s Republic of China Chinese Academy of Sciences Institute of BotanyBeijingPeople’s Republic of China Royal Botanic Gardens KewRichmondUK School of International Economics and Management Beijing Technology and Business UniversityBeijingPeople’s Republic of China. Satellite Environmental Application Center Ministry of Ecology and EnvironmentBeijingPeople’s Republic of China. University of Chinese Academy of Sciences BeijingPeople’s Republic of China

Based on the two most recent assessments of Chinese higher plants in 2013 and 2020,of 34,450 and 39,330 species,respectively,we analysed the threatened status of Chinese higher *** 2020,around 4,088(10.39%)of the assessed species in China are threatened,2,875(7.31%)Near Threatened,27,593(70.16%)not currently threatened and categorised as Least Concern and 4,752(12.08%)categorised as Data *** in 2013,3,767(10.93%)of the assessed higher plants in China are threatened,2,723(7.90%)Near Threatened,24,296(70.53%)Least Concern and 3,612(10.48%)Data *** of the Red List Index in the two years show different patterns when using different weighting methods with the equal steps weighting method showing a slight decrease(0.91675-0.91495)and the extinction risk weighting method showing a slight increase(0.98792-0.98797).We inferred that China's threatened plant species were likely/relatively effectively ***,attention should also be given to the non-threatened species in the future as an additional strategy for their conservation,to maintain their non-threatened status.

关键词： Chinese higher plants conservation IUCN red list assessment red list index(RLI) sustainable development goals(SDGs)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：