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A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine

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Journal of Bionic Engineering 2022年第4期19卷 1109-1123页

作者： Peng Bai Gangcheng Kong Weihua Qiao Yu Song Yixuan Wang Jiawei Shi Nan Zhang Chungeng Liu Chong Chu Tixiusi Xiong Ying Zhou Cuifen Lu Lin Wang nianguo dong Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430032China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Research Center for Tissue Engineering and Regenerative Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Endocrinology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430032China Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry Hubei UniversityWuhan 430062China Department of Clinical Laboratory Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Cardiac valve replacement is an effective method to treat valvular heart disease.Artificial valves used routinely in clinic still have defects.In our study,we explored a novel method to modify the performance of Decellularized Heart Valve(DHV)scaffold.The decellularized porcine aortic valve was prepared using sequential hydrophile and lipophile solubilization method.The sericin was extracted from silk fibroin-deficient silkworm cocoon by lithium bromide method.First,DHV was immersed in sericin solution to produce the sericin–DHV composite scaffold.Then,we modified the DHV by making a Polydopamine(PDA)coating on the DHV first and then binding the sericin.The physical properties and biological compatibility of our composite scaffold were assessed in vitro and in vivo.Sericin were successfully prepared,combined to DHV and improved its biocompatibility.PDA coating further promoted the combination of sericin on DHV and improved the physical properties of scaffolds.The decay rate of our modified valve scaffold was decreased in vivo and it showed good compatibility with blood.In conclusion,our modification improved the physical properties and biocompatibility of the valve scaffold.The combination of PDA and sericin promoted the recellularization of decellularized valves,showing great potential to be a novel artificial valve.

关键词： Decellularized heart valve Sericin Polydopamine Biocompatibility

Current progress on scaffolds of tissue engineering heart valves

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Frontiers of Medicine 2008年第3期2卷 229-234页

作者： nianguo dong Jiawei SHI Ping HU Si CHEN Hao HONG Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Polymer Science&Engineering Tsinghua UniversityBeijing 100084China

Tissue engineering heart valves(TEHV)may be the most promising valve substitute,but the study has been relatively stagnant in the recent five years due to the special position,function and mechanical property of heart valves.It is one of the key factors to select an ideal scaffold material in the construction of TEHV.And this article will briefly review the current research and progress on the scaffolds of TEHV,especially based on Chinese works.

关键词： tissue engineering heart valve scaffold

Experimental and computational models for tissue-engineered heart valves:a narrative review

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Biomaterials Translational 2021年第4期2卷 361-375页

作者： Ge Yan Yuqi Liu Minghui Xie Jiawei Shi Weihua Qiao nianguo dong Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Valvular heart disease is currently a common problem which causes high morbidity and mortality worldwide.Prosthetic valve replacements are widely needed to correct narrowing or backflow through the valvular orifice.Compared to mechanical valves and biological valves,tissue-engineered heart valves can be an ideal substitute because they have a low risk of thromboembolism and calcification,and the potential for remodelling,regeneration,and growth.In order to test the performance of these heart valves,various animal models and other models are needed to optimise the structure and function of tissue-engineered heart valves,which may provide a potential mechanism responsible for substantial enhancement in tissue-engineered heart valves.Choosing the appropriate model for evaluating the performance of the tissue-engineered valve is important,as different models have their own advantages and disadvantages.In this review,we summarise the current state-of-the-art animal models,bioreactors,and computational simulation models with the aim of creating more strategies for better development of tissue-engineered heart valves.This review provides an overview of major factors that influence the selection and design of a model for tissue-engineered heart valve.Continued efforts in improving and testing models for valve regeneration remain crucial in basic science and translational researches.Future research should focus on finding the right animal model and developing better in vitro testing systems for tissue-engineered heart valve.

关键词： animal model bioreactor computational modelling tissue-engineered heart valve

A retrospective cohort study of methylprednisolone therapy in severe patients with COVID-19 pneumonia

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1979-1981页

作者： Yin Wang Weiwei Jiang Qi He Cheng Wang Baoju Wang Pan Zhou nianguo dong Qiaoxia Tong Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Rheumatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Hand Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China

Dear Editor,Corona Virus Disease 2019(COVID-19)was first reported in late December 2019,in Wuhan,China.There are over 1,800,000 confirmed cases worldwide.1 The pathological process of severe COVID-19 pneumonia is an inflammation reaction characterized by the destruction of the deep airway and alveolar.2 It is currently considered that lung injury is not only associated with the direct virus-induced damage,but also the immune responses triggered by COVID-19 that lead to the activation of immune cells to release a large number of pro-and anti-inflammatory cytokines.Histologic examination has shown diffuse alveolar damage and mucinous exudate,which is similar to acute respiratory distress syndrome.2 Aggravation of symptoms always occurs during 5–7 days after onset in patients with COVID-19 pneumonia and severe cases develop rapidly to acute respiratory failure.3 Therefore,it is important to strengthen the treatment to suppress the proinflammatory response and control the cytokine storm at this stage.Methylprednisolone are the classical immunosuppressive drugs,which are important to stop or delay the progress of the pneumonia,and have been proved to be effective for the treatment of acute respiratory distress syndrome(ARDS).

关键词： pneumonia damage drugs

A Rare Long Term Survival Case of Complete Pentalogy of Cantrell in Adult after One-Staged Surgery

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Congenital Heart Disease 2021年第5期16卷 513-518页

作者： Hao Hong nianguo dong Mingxing Xie Lin Li Si Chen Wengang Sun Xiaoqing Hu Department of Cardiovarscular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Ultrasound Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Radiology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Pentalogy of Cantrell is a rare congenital abnormality.Even with high-quality medical care and staged corrective surgeries in professional hospitals,the mortality rate is still very high and long-term prognosis is poor.Survival is largely determined by the complexity and severity of cardiac abnormalities,the efficiency of abdominal wall closure and postoperative complications.A 24-year-old male patient with complete pentalogy of Cantrell was diagnosed.One-stage surgical repair of all abnormalities were completed.The patient recovered well and had been follow-up for 7 years.He has a good cosmetic outcome,with no signs of cardiac dysfunction.No complications were noted.

关键词： Pentalogy of cantrell adult one-stage surgery

Clinical application and management of temporary mechanical circulatory support: A clinical consensus

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中华医学杂志(英文版) 2024年第10期137卷 2023 Dec 26页

作者： dong nianguo Chen Liangwan Chen Xin Wang Huishan Xiao Yingbin Wang Xianqiang Huang Jinsong Yan Yang Bonde Pramod Sodha Neel Atluri Pavan Maureira Juan Pablo Vincentelli André Yanase Masanobu Wang Yixuan Zhang Haitao Hou Xiaotong Takami Yoshijuki Leprince Pascal Folliguet Thierry Sellke Frank W Chinese Society for Thoracic and Cardiovascular Surgery Chinese Association of Cardiovascular Surgeons Cardiac Critical Care Professional Committee of Chinese Medical Association Chinese Society of Extracorporeal Life Support European Society of Cardiovascular and Endovascular Surgery French Society of Thoracic and Cardiovascular Surgery Japanese Society for Cardiovascular Surgery Japanese Society of Artificial Organs Department of Cardiothoracic Surgery Alpert Medical School Brown University Rhode Island Hospital Providence RI USA Department of Cardiovascular Surgery Department of Surgery University of Pennsylvania Philadelphia PA USA Department of Cardiovascular Surgery and Transplantation University Hospital of Nancy University of Lorraine Vandoeuvre-lès-Nancy France Department of Cardiovascular Surgery University Hospital of Lille University of Lille Lille France Department of Cardiology Fujita Health University School of Medicine Toyoake Aichi-1192 Japan Department of Adult Surgical Intensive Care Unit Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Department of Cardiac Intensive Care Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing Anzhen Hospital Capital Medical University Beijing China Department of Cardiovascular Surgery Fujita Health University School of Medicine Toyoake Aichi-1192 Japan Department of Cardiovascular Surgery Groupe Hospitalier Pitié-Salpêtrière University Pierre et Marie Curie APHP Paris France Department of Cardiac Surgery APHP H?pitaux Universitaires Henri Mondor Créteil F-94010 France Department of Cardiovascular Surgery Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei China Department of Cardiovascular Surgery Fujian Medical University Union Hospital Fuzhou Fujian China Department of Thoracic and Cardiovascular Surgery Nanjing First Hospital Nanjing Medical University Nanjing Jiangsu China Department of Cardiovascular Surgery General Hospital of Northern Theater Command Shenyang Liaoning China Department of Cardiovascular Surgery Xinqiao Hospital Third Military Medical University Chongqing China Department of Cardiovascular Surgery Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing Chi

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小鼠心肌缺血再灌注损伤模型改良及评价

中华胸心血管外科杂志 2024年第4期40卷 214-221页

作者：宋远王一卉高瑭董念国谢明星华中科技大学同济医学院附属协和医院超声医学科武汉430022 湖北省影像医学临床研究中心武汉430022 分子影像湖北省重点实验室武汉430022 华中科技大学同济医学院附属协和医院心脏大血管外科武汉430022

目的改良小鼠心肌缺血再灌注损伤(MIRI)造模方法,并对其系统性评价。方法将70只SPF级雄性C57BL/6小鼠随机分为假手术组(Sham)、经典组(CM)、单圈活结组(SM)和改良活结组(MM)。通过结扎冠状动脉左前降支30 min后再灌注24 h建立MIRI模型,... 详细信息

目的改良小鼠心肌缺血再灌注损伤(MIRI)造模方法,并对其系统性评价。方法将70只SPF级雄性C57BL/6小鼠随机分为假手术组(Sham)、经典组(CM)、单圈活结组(SM)和改良活结组(MM)。通过结扎冠状动脉左前降支30 min后再灌注24 h建立MIRI模型,记录并统计各组小鼠建模时间、模型存活率及实时心电图的变化。采用2,3,5-氯化三苯基四氮唑(TTC)、HE、Masson和TUNEL染色,比较MIRI心肌组织病理改变。生化仪测定血清谷草转氨酶(AST)、肌酸激酶(CK)、肌酸激酶同工酶MB(CK-MB)和乳酸脱氢酶(LDH)水平。ELISA法分析肿瘤坏死因子α(TNF-α)、白细胞介素2(IL-2)、白细胞介素6(IL-6)和单核细胞趋化因子1(MCP-1)水平。结果MM组小鼠心电图ST段明显抬高;MM组建模时间(36.2 min)显著少于CM组(66.2 min,P<0.0001),且MM组相较于CM组存活率显著提升(P<0.05)。MM组小鼠心肌组织梗死区细胞肿胀变形,心肌纤维化程度高于其他组并明显发生凋亡。CM组和MM组小鼠心肌酶AST、CK、CK-MB、LDH水平较Sham组均显著升高,其中MM组的CK和CK-MB水平较CM组明显增加(P<0.05)。CM组和MM组小鼠炎性因子TNF-α(P<0.01)、IL-6(P<0.05)较Sham组显著升高,CM组IL-2水平显著高于Sham组和MM组(P<0.05),MCP-1水平各组间差异无统计学意义。结论与经典和单圈活结造模方式相比,利用改良活结法建立的小鼠MIRI模型,造模时间缩短,模型更稳定,小鼠存活率显著提高。

关键词：心肌缺血再灌注损伤结扎动物模型小鼠

Expert consensus on the use of human serum albumin in adult cardiac surgery

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Chinese Medical Journal 2023年第10期136卷 1135-1143页

作者： Fei Xiang Fuhua Huang Jiapeng Huang Xin Li nianguo dong Yingbin Xiao Qiang Zhao Liqiong Xiao Haitao Zhang CuiZhang Zhaoyun Cheng Liangwan Chen Jimei Chen Huishan Wang Yingqiang Guo Nan Liu Zhe Luo Xiaotong Hou Bingyang Ji Rong Zhao Zhenxiao Jin Robert Savage Yang Zhao Zhe Zheng Xin Chen on behalf of Chinese Association of Cardiovascular Surgeons Chinese Society of Thoracic and Cardiovascular Surgery Chinese Society of Cardiothoracic and Vascular Anesthesiology Department of Thoracic and Cardiovascular Surgery Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China Department of Anesthesiology and Perioperative Medicine University of LouisvilleLouisvilleKentucky 40292United States Department of Cardiovascular Surgery Zhongshan HospitalFudan UniversityShanghai 200031China Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430000China Department of Cardiovascular Surgery the Second Affiliated HospitalArmy Medical UniversityChongqing 400037China Department of Cardiovascular Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200031China Department of Critical Care Fuwai HospitalChinese Academy of Medical SciencesBeijing 100033China Department of Critical Care Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China Department of Cardiac Surgery People’s Hospital of Henan ProvincePeople’s Hospital of Zhengzhou UniversityFuwai Central China Cardiovascular Disease HospitalZhengzhouHenan 450003China Department of Cardiac Surgery Fujian Medical University Union HospitalFuzhouFujian 350001China Department of Cardiovascular Surgery Guangdong Cardiovascular InstituteGuangdong Provincial Key Laboratory of South China Structural Heart DiseaseGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouGuangdong 510050China Department of Cardiovascular Surgery General Hospital of Shenyang Military Area CommandShenyangLiaoning 110055China Department of Cardiovascular Surgery West China Hospital of Sichuan UniversityChengduSichuan 610041China Center for Cardiac Intensive Care Beijing Anzhen HospitalBeijing Institute of HeartLung and Blood Vessel DiseasesCapital Medical UniversityBeijing 102218China Department of Cardiopulmonary Bypass Fuwai HospitalChinese Academy of Medical SciencesBeijing 100033China Department of Cardiovascular Surgery Xijing HospitalFourth Militar

Introduction Albumin,which is a 65-kDa liver-synthesized protein,accounts for nearly 50%of total plasma protein and contributes to approximately 80%of intravascular oncotic pressure.Albumin helps maintain microvascular integrity,functions as an antioxidant,and transports hormones,fatty acid,bile salts,bilirubin,electrolytes(e.g.,calcium,magnesium,copper,zinc,et al.),and drugs.[1–3]Human serum albumin(HSA)is a sterile,liquid albumin product derived from large pools of human plasma by fractionation and pasteurization.The medical use of HAS could date back to approximately the time of World War II.

关键词： Albumin drugs surgery

精准外科理念在心脏移植围手术期管理中的应用

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实用器官移植电子杂志 2024年第3期12卷 262-265页

作者：胡修齐乔韡华张菁董念国华中科技大学同济医学院附属协和医院心脏大血管外科湖北武汉430022

心脏移植是目前终末期心脏病最重要的治疗方法[1-3]。经过多年的发展,心脏移植技术日臻成熟,术后并发症发生率不断降低,远期生存率不断延长。尽管如此,心脏移植的整体疗效仍被供心保护效果有限、特殊受体移植技术困难、免疫排斥反应与... 详细信息

心脏移植是目前终末期心脏病最重要的治疗方法[1-3]。经过多年的发展,心脏移植技术日臻成熟,术后并发症发生率不断降低,远期生存率不断延长。尽管如此,心脏移植的整体疗效仍被供心保护效果有限、特殊受体移植技术困难、免疫排斥反应与其他并发症复杂多发等难题掣肘。实现准确的术前评估、合适的术中规划以及细致的术后管理是提升心脏移植质量的关键。随着诊疗技术不断进步,以术前精准评估、术中精细操作和术后加速康复为核心的“精准外科”理念逐步渗透诸多外科领域,成为外科医学发展的必然趋势[4],也正与心脏移植对围手术期各环节的严格要求不谋而合。

关键词：围手术期管理精准外科心脏移植外科医学远期生存率免疫排斥反应术前评估终末期心脏病