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Geographical distribution of GmTfl1 alleles in Chinese soybean varieties

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The Crop Journal 2015年第5期3卷 371-378页

作者： Guifeng Liu lin Zhao benjamin J.Averitt Ying Liu bo Zhang Ruzhen Chang Yansong Ma Xiaoyan Luan Rongxia Guan Lijuan Qiu Soybean Research Institute Northeast Agricultural University Department of Crop and Soil Environmental Sciences VA Polytechnic Institute and State University The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences Heilongjiang Academy of Agricultural Sciences

Stem growth habit is an important agronomic trait in soybean and is subject to artificial selection. This study aimed to provide a theory for genotypic selection of stem growth habit for breeding purposes by analyzing the alleles of Gm Tfl1 gene in Chinese soybean varieties and establishing a database of Gm Tfl1 variation. Using knowledge of insertion and deletion(Indel) in the non-coding region and four single-nucleotide polymorphisms(SNPs) in the coding sequences of the Gm Tfl1 gene, four CAPS and one Indel markers were developed and used to test 1120 Chinese soybean varieties. We found that the dominant Gm Tfl1 allele was prevalent in accessions from the Northern ecoregion, whereas the recessive allele, Gmtfl1, was more common in the Southern ecoregion, and the proportions of Gm Tfl1 and recessive alleles were respectively 40.1% and 59.9% in the Huang-Huai ecoregion. The proportion of Gm Tfl1 decreased and that of Gmtfl1 increased, gradually from north to south. Allele Gm Tfl1-a was present in higher proportions in the Huang-Huai spring, Huang-Huai summer, and Northern spring sub-ecoregions than that in the other sub-ecoregions. Gm Tfl1-b was common in the Northeast spring, Northern spring and Southern summer sub-ecoregions. Gmtfl1-ta was found mainly in the Huang-Huai spring,Huang-Huai summer and Southern spring sub-ecoregions. The Gmtfl1-ab allele was distributed in all six soybean sub-ecoregions. The Gmtfl1-bb allele was distributed mainly in the Huang-Huai spring and summer and Southern spring and summer sub-ecoregions,but the Gmtfl1-tb allele was detected only in the Huang-Huai summer sub-ecoregion. The distributions of Gm Tfl1 and Gmtfl1 have shown no large changes in nearly 60 years of breeding, but the frequency of the recessive genotype Gmtfl1 has shown a rising trend in the last 20 years. This study provides a theoretical foundation for breeding new soybean varieties for different ecoregions.

关键词： Soybean Stem growth habit Gm Tfl1 Distribution

Thermoacoustic assessment of hematocrit changes in human forearms

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Chinese Physics b 2021年第9期30卷 284-289页

作者： Xue Wang Rui Zhao Yi-Tong Peng Zi-Hui Chi Zhu Zheng En Li lin Huang Hua-bei Jian School of Electronic Science and Engineering(National Exemplary School of Microelectronics) University of Electronic Science and Technology of ChinaChengdu 611731China Center for Information in Medicine University of Electronic and Technology of ChinaChengdu 611731China Department of Medical Engineering University of South FloridaTampa FL 33620USA School of Optoelectronic Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China

Abnormal hematocrit(Hct)is associated with an increased risk of pre-hypertension and all-cause death in general population,and people with a high Hct value are susceptible to arterial cardiovascular disease and venous *** this study,we report for the first time on the ability of thermoacoustic imaging(taI)for in vivo evaluating Hct changes in human *** vitro blood samples with different Hct values from healthy volunteers(n=3)were prepared after *** was performed using these samples in comparison with the direct measurements of *** vivo taI was conducted in the forearm of healthy volunteers(n=7)where Hct changes were produced through a vascular occlusion stimulation over a period of *** results of in vitro blood samples obtained from the 3 healthy subjects show that the thermoacoustic(ta)signals changes due to the variation of blood conductivity are closely related to the changes in *** addition,the in vivo ta signals obtained from the 7 healthy subjects consistently increase in the artery/muscle and decrease in the vein during venous or arterial occlusion because of the changed Hct value in their *** findings suggest that taI has the potential to become a new tool for monitoring Hct changes for a variety of pre-clinical and clinical applications.

关键词： thermoacoustic imaging hematocrit change human forearm

Co-rrp基因小干扰RNA载体的构建及烟草中的遗传转化

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中南林业科技大学学报 2010年第10期30卷 68-71,77页

作者：包梅荣谭晓风乌云塔娜张琳中南林业科技大学经济林育种与栽培国家林业局重点实验室中南林业科技大学林学院国家油茶科学中心生物技术实验室湖南长沙410004

为研究油茶种子成熟调控蛋白基因的功能,以pCAMbIA1304质粒为载体,根据油茶种子成熟调控蛋白基因的cDNA序列设计了小干扰RNA(Si RNA)序列,构建了油茶种子成熟调控蛋白基因小干扰RNA植物表达载体pCAMbIA1304-si RNA,并借助农杆菌介导叶... 详细信息

为研究油茶种子成熟调控蛋白基因的功能,以pCAMbIA1304质粒为载体,根据油茶种子成熟调控蛋白基因的cDNA序列设计了小干扰RNA(Si RNA)序列,构建了油茶种子成熟调控蛋白基因小干扰RNA植物表达载体pCAMbIA1304-si RNA,并借助农杆菌介导叶盘法将pCAMbIA1304-si RNA转化到野生型烟草中,获得了稳定表达的转基因烟草苗。为下一步的油茶种子成熟调控蛋白基因的功能鉴定奠定了基础。

关键词：油茶种子成熟调控蛋白基因植物表达载体小干扰RNA 转基因烟草

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热膨胀法测定玻璃热性能的应用评估

玻璃 2024年第1期51卷 19-23页

作者：柏晓强曹国荣邢文忠章林洪秀成赵刚巨石集团有限公司桐乡314500

热膨胀法可以测定玻璃的热性能,包括:热膨胀系数α与应变点T_(st)、转变点T_(g)、退火点T_(a)、膨胀软化点T_(d)等特征温度点。对热膨胀法测定玻璃热性能的应用进行评估,评估结果表明:(1)玻璃的热膨胀系数α会随着温度段变化而变化,同... 详细信息

热膨胀法可以测定玻璃的热性能,包括:热膨胀系数α与应变点T_(st)、转变点T_(g)、退火点T_(a)、膨胀软化点T_(d)等特征温度点。对热膨胀法测定玻璃热性能的应用进行评估,评估结果表明:(1)玻璃的热膨胀系数α会随着温度段变化而变化,同时在一定范围内,升温速率越大,热膨胀系数越小;(2)热膨胀法测定玻璃特征温度点的重复性偏差在±10℃以内,一定范围内,升温速率越大,特征温度点温度越高;(3)热膨胀法测定玻璃热性能的升温速率优选3℃/min;(4)玻璃中b_(2)O_(3)含量越高,同时CaO含量越低,那么玻璃的热膨胀系数α越小,玻璃的应变点T_(st)与转变温度T_(g)越小,退火加工温度范围也就越大。

关键词：玻璃热膨胀系数玻璃转变点膨胀软化点退火

基于超声辅助的血管分级管理在治疗性单采患者中的应用效果分析

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现代医药卫生 2024年第18期40卷 3132-3136页

作者：石洁许志强陆婧媛林玉凤蔡志云厦门大学附属中山医院血液科福建厦门361004 厦门大学附属中山医院静脉治疗专科门诊福建厦门361004

目的探讨基于超声辅助的血管分级管理在治疗性单采(ta)患者中的应用效果。方法选取2019年1月至2023年11月在厦门市某三甲医院血液科行ta的174例患者为研究对象,根据治疗时间分为观察组(2022年1月至2023年11月)和对照组(2019年1月至202... 详细信息

目的探讨基于超声辅助的血管分级管理在治疗性单采(ta)患者中的应用效果。方法选取2019年1月至2023年11月在厦门市某三甲医院血液科行ta的174例患者为研究对象,根据治疗时间分为观察组(2022年1月至2023年11月)和对照组(2019年1月至2021年12月),各87例。对照组患者由ta团队根据个人经验置入静脉通路,观察组患者则基于b超辅助进行血管分级管理。比较2组患者在一次性穿刺成功率、中心静脉导管(CVC)使用率、静脉导管并发症、采集相关不良反应及静脉导管费用、患者满意度方面的差异。结果 2组患者均完成ta,观察组患者一次性穿刺成功率为93.1%,高于对照组的81.6%,差异有统计学意义(P0.05)。结论基于超声辅助的血管分级管理能提高ta患者一次性置管成功率及患者满意度,减少ta患者的CVC插管率及插管费用。

关键词：超声辅助外周血管装置血管分级管理治疗性单采中心静脉导管

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广东曲江罗坑自然保护区广东松群落特征

武汉植物学研究 2008年第1期26卷 53-58页

作者：王厚麟缪绅裕吴伟东薛锡塔金建华广州大学华南生物多样性研究所广州510006 中山大学生命科学学院广州510275

应用植被样地调查法研究了广东曲江罗坑省级自然保护区的广东松群落的物种组成、科属地理成分和种群年龄结构,为国家保护植物广东松的保育提供基础资料。结果表明,3400m2样方中有维管植物237种,隶属于74科126属。群落中维管植物的数量... 详细信息

应用植被样地调查法研究了广东曲江罗坑省级自然保护区的广东松群落的物种组成、科属地理成分和种群年龄结构,为国家保护植物广东松的保育提供基础资料。结果表明,3400m2样方中有维管植物237种,隶属于74科126属。群落中维管植物的数量优势科为壳斗科(Fagaceae)、樟科(Lauraceae)、杜鹃花科(Ericaceae)、蔷薇科(Rosaceae)、山茶科(Theaceae)和冬青科(Aquifoliaceae);优势种为广东松(Pinus kwangtungensis)、疏齿木荷(Schima remotiserrata)、猴头杜鹃(Rhododendron simiarum)和五列木(Pentaphylax euryoides)。植物科、属的地理成分以泛热带和热带亚洲分布型为主,体现了南亚热带向中亚热带过渡的特点。种群年龄结构分析表明,建群种广东松接近衰退种群阶段,而优势种疏齿木荷、猴头杜鹃和五列木等属于基本稳定种群,倒卵叶青冈(Cyclobalanopsis obovatifolia)和小叶罗汉松(Podocarpus macrophyllus ***)的更新幼苗数量多,属于典型的增长种群。

关键词：广东松群落特征曲江罗坑自然保护区

Effect of heavy ion irradiation on the interface traps of AlGaN/GaN high electron mobility transistors

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Chinese Physics b 2022年第3期31卷 428-433页

作者： Zheng-Zhao lin ling Lü Xue-Feng Zheng Yan-Rong Cao Pei-Pei Hu Xin Fang Xiao-Hua Ma School of Microelectronics Xidian UniversityXi'an 710071China School of Mechano-Electronic Engineering Xidian UniversityXi'an 710071China Institute of Modern Physics Chinese Academy of Sciences (CAS)Lanzhou 730000China

AlGaN/GaN high electron mobility transistors(HEMTs)were irradiated with heavy ions at various *** irradiation by 2.1 GeV^(181) ta^(32+) ions,the electrical characteristics of the devices significantly *** threshold voltage shifted positively by approximately 25%and the saturation currents decreased by approximately 14%.Defects were induced in the band gap and the interface between the gate and barrier acted as tunneling sites,which increased the gate current tunneling *** to the pulsed output characteristics,the amount of current collapse significantly increased and more surface state traps were introduced after heavy ion *** time constants of the induced surface traps were mainly less than 10μs.

关键词： gallium nitride radiation effects defects pulse testing

树轮宽度记录的塔吉克斯坦北部160 a平均最低气温变化

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干旱区研究 2019年第2期36卷 290-295页

作者：杨美琳他志杰吴燕良赵鹏于瑞德中国科学院新疆生态与地理研究所荒漠环境研究室新疆乌鲁木齐830011 中国科学院大学北京100049

利用塔吉克斯坦北部土耳其斯坦圆柏(Juniperus turkestanica)树轮宽度标准化年表与区域气候要素进行相关分析,发现当年2—3月平均最低气温与树木径向生长存在显著相关关系。据此对该地区1857—2016年2—3月平均最低气温进行重建,重建方... 详细信息

利用塔吉克斯坦北部土耳其斯坦圆柏(Juniperus turkestanica)树轮宽度标准化年表与区域气候要素进行相关分析,发现当年2—3月平均最低气温与树木径向生长存在显著相关关系。据此对该地区1857—2016年2—3月平均最低气温进行重建,重建方程的解释量为39. 5%,调整自由度后的解释量为37. 5%。研究表明:在过去160 a,该地区经历了6个偏暖阶段,1857—1878年、1885—1890年、1907—1915年、1926—1931年、1960—1967年、1994—2016年; 5个偏冷阶段,1879—1884年、1891—1906年、1916—1925年、1932—1959年、1968—1993年。空间分析结果表明,研究区2—3月平均最低气温的重建结果可以代表塔吉克斯坦地区过去160 a的2—3月平均最低气温的变化。

关键词：树木年轮宽度年表月平均最低气温土耳其斯坦圆柏(Juniperus turkestanica) 塔吉克斯坦

taSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat

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Journal of Integrative Agriculture 2021年第1期20卷 46-54页

作者： MIAO Li-li LI Yu-ying ZHANG Hong-juan ZHANG Hong-ji LIU Xiu-lin WANG Jing-yi CHANG Xiao-ping MAO Xin-guo JING Rui-lian Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme Harbin 150086P.R.China Institute of Crop Resources Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Soybean Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China

Sucrose non-fermenting 1-related protein kinase 2(Sn RK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli. Previous studies showed that ta Sn RK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis. Further experiments were performed to decipher the underlying mechanisms and discover new functions. The genomic sequences of ta Sn RK2.4 s locating on chromosome 3 A, 3 b and 3 D were obtained. Sequencing identified one and 13 variations of ta Sn RK2.4-3 A and ta Sn RK2.4-3 b, respectively, but no variation was detected in ta Sn RK2.4-3 D. The markers 2.4 AM1, 2.4 bM1 and 2.4 bM2 were developed based on three variations. Association analysis showed that both ta Sn RK2.4-3 A and ta Sn RK2.4-3 b were significantly associated with thousand-kernel weight(TKW), and that SNP3 A-T and SNP3 b-C were favorable alleles for higher TKW. Yeast two-hybrid and split luciferase assays showed that ta Sn RK2.4 physically interacted with abiotic stress responsive protein ta LTP3, suggesting that ta Sn RK2.4 enhanced abiotic stress tolerance by activating ta LTP3. Our studies suggested that ta Sn RK2.4 have potential in improving TKW and response to abiotic stress.

关键词： taSnRK2.4 association analysis TKW abiotic stress