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蝉花多糖酶法辅助双水相提取工艺优化及其抗氧化、降血糖和降血脂活性分析

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食品工业科技 2024年第12期45卷 179-188页

作者：李金婷钱心燚雍一丹吴萌萌孙华锴王雅楠陈安徽邵颖尼再中徐州工程学院食品与生物工程学院江苏徐州221018

蝉花多糖是蝉花主要成分之一,具有多种生物活性。本研究以蝉花子实体为原料,采用纤维素酶和果胶酶辅助双水相体系提取蝉花多糖,以期为蝉花多糖的进一步开发利用和深入研究提供参考。通过单因素实验分析了纤维素酶添加量、果胶酶添加量、... 详细信息

蝉花多糖是蝉花主要成分之一,具有多种生物活性。本研究以蝉花子实体为原料,采用纤维素酶和果胶酶辅助双水相体系提取蝉花多糖,以期为蝉花多糖的进一步开发利用和深入研究提供参考。通过单因素实验分析了纤维素酶添加量、果胶酶添加量、(NH_(4))2 SO_(4)质量分数以及聚乙二醇(polyethylene glycol,PEG)质量分数对蝉花多糖得率的影响。采用响应面法优化了蝉花多糖的提取工艺,并对其体外抗氧化、降血糖和降血脂生物活性进行了研究。结果表明,蝉花多糖的最优提取条件为:纤维素酶添加量2.01%,果胶酶添加量2.07%,(NH_(4))2 SO_(4)质量分数17.72%,PEG质量分数20.87%,此条件下蝉花多糖得率达到25.34%。在1.0~5.0 mg/mL浓度范围内,蝉花多糖的Fe2+螯合率和ABTS+自由基清除率最大值分别为16.33%±1.68%和17.38%±3.07%。总还原力测定表明蝉花多糖吸光度最大值为0.53±0.005。另外,蝉花多糖对α-葡萄糖苷酶和α-淀粉酶活性的最大抑制率分别为30.96%±4.53%和49.40%±1.30%。对胆酸钠、牛磺胆酸钠和胆固醇的最大吸附率分别为47.24%±1.89%,44.88%±0.82%和70.19%±2.54%。综上,蝉花多糖具有显著的抗氧化、降血糖和降血脂活性。本研究将为蝉花多糖相关产品的开发,特别是临床应用提供一定的理论依据。

关键词：蝉花多糖提取优化抗氧化降血糖降血脂

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Observation of intra-group and extra-group copulation and reproductive characters in free ranging groups of western black crested gibbon(Nomascus concolor jingdongensis)

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Integrative Zoology 2013年第4期8卷 427-440页

作者： Bei HUANG Zhenghua GUAN Qingyong ni Joseph D.ORkIN Pengfei FAN Xuelong JIANG State key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnanChina Graduate School of Chinese Academy of Sciences BeijingChina College of Animal Science and Technology Sichuan Agriculture UniversityYa’anSichuanChina Department of Anthropology Washington University in St.LouisSt.LouisMissouriUSA Institute of Eastern-Himalaya Biodiversity Research Dali UniversityDaliYunnanChina

Black crested gibbons(Nomascus concolor)are 1 of only 3 gibbon species that have been observed in long-term polygynous groups,but their mating behavior and reproductive characters have never been *** on population monitoring over 7 years and direct observation for 26 months of the study groups in Wuliang Mountain,central Yunnan,we describe for the first time the copulation behavior and breeding pattern of free-ranging western black crested *** gestation period of black crested gibbons is estimated to be between 6 and 7 *** average breeding interval is 3.5 years,with infant independence at approximately 2.5 *** observed 2 intra-group copulations and 5 extra-group *** were initiated when a female gave a‘solicitation’*** a male made any mating attempt,the female might refuse *** results demonstrate direct female mate *** male and female gibbons dispersed from their natal groups and sometimes replaced paired adults in other *** observed no evidence of infanticide during inter-group conflicts or after replacement of *** with extra-group copulations,these phenomena indicate a flexible social organization and complex mating *** also observed a male-biased sex ratio among *** genetic work is necessary to describe the effects of inter-group copulation and the genetic diversity of this population.

关键词： copulation behavior mating system Nomascus concolor reproductive characters Wuliang Mountain

A nondestructive method for estimating the total green leaf area of individual rice plants using multi-angle color images

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Journal of Innovative Optical Health Sciences 2015年第2期8卷 7-18页

作者： ni Jiang Wanneng Yang Lingfeng Duan Guoxing Chen Wei Fang Lizhong Xiong Qian Liu Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology 1037 Luoyu Rd. Wuhan 430074P.R.China National key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Huazhong Agricultural University Wuhan 430070 P.R.China College of Engineering Huazhong Agricultural University Wuhan 430070 P.R.China MOA key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China

Total green leaf area(GLA)is an important trait for agronomic ***,existing methods for estimating the GLA of individual rice plants are destructive and labor-intensive.A nondestructive method for estimating the total GLA of individual rice plants based on multi-angle color images is *** projected areas of the plant in images,linear,quadratic,exponential and power regression models for estimating total GLA were *** demonstrated that the side-view projected area had a stronger relationship with the actual total leaf area than the top-projected *** power models fit better than other *** addition,the use of multiple side-view images was an efficient method for reducing the estimation *** inclusion of the top-view projected area as a seoond predictor provided only a slight improvement of the total leaf area est *** the projected areas from multi angle images were used,the estimated leaf area(ELA)using the power model and the actual leaf area had a high correlation cofficient(R2>0.98),and the mean absolute percentage error(MAPE)was about 6%.The method was capable of estimating the total leaf area in a nondestructive,accurate and eficient manner,and it may be used for monitoring rice plant growth.

关键词： Agri photonics image processing plant phenotyping regression model visible light imaging

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猴头菌培养液乙酸乙酯提取物的化学成分研究

湖南中医药大学学报 2024年第2期44卷 245-250页

作者：姚清颖张小娟曾妮付港李斌王炜湖南中医药大学药学院湖南长沙410208 湖南中医药大学中医药民族医药国际联合实验室湖南长沙410208

目的研究猴头菌培养液乙酸乙酯提取物的化学成分。方法通过凝胶柱色谱、硅胶柱色谱、半制备高效液相色谱等技术进行分离和纯化;根据其理化性质数理和波谱对得到的化合物进行结构鉴定。结果从猴头菌培养液乙酸乙酯提取物中分离并鉴定得... 详细信息

目的研究猴头菌培养液乙酸乙酯提取物的化学成分。方法通过凝胶柱色谱、硅胶柱色谱、半制备高效液相色谱等技术进行分离和纯化;根据其理化性质数理和波谱对得到的化合物进行结构鉴定。结果从猴头菌培养液乙酸乙酯提取物中分离并鉴定得到22个化合物,分别为3-甲氧基-4-羟基苯甲醛(1),吲哚甲酸甲酯(2),2-呋喃甲酸(3),苯甲酸(4),2(3H)-苯并恶唑酮(5),香草酸(6),没食子酸(7),polisin C(8),bis(2-ethylhexyl)benzene-1,2-dicarboylate(9),p-tyrosyl acetate(4-hydroxyphenethyl acetate)(10),1-(4-hydroxy-3,5-dimethoxyphenyl)ethanon(11),对羟基苯乙酮(12),1-苯基-1,2-丙二醇(13),6-羟基异苯并呋喃-1(3H)-酮(14),对羟基苯乙醛(15),1H-吲哚-3-吡咯甲醛(16),环(脯氨酸-缬氨酸)(17),环(脯氨酸-亮氨酸)(18),环(苯丙氨酸-缬氨酸)(19),环(异亮氨酸-苯丙氨酸)(20),苜蓿素(21),dihydro-4-hydroxy-2(3H)-furanone(22)。结论除化合物16和21外,其他化合物均为首次从猴头菌培养液中分离得到。

关键词：猴头菌培养液化学成分小分子化合物环肽类化合物生物碱

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固本祛湿化瘀方对小鼠银屑病复发模型皮损和脾脏水转运相关物质的影响

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中国中西医结合杂志 2024年第1期44卷 63-69页

作者：张慧玲刘丽娟韦妮吴亚运赵亚卢传坚赵瑞芝广州中医药大学第二附属医院省部共建中医湿证重点实验室广州510006 广州中医药大学第二临床医学院广州510006

目的探讨固本祛湿化瘀方(GQHF)治疗及延缓银屑病复发的可能作用机制。方法42只Balb/c小鼠按随机数字表法分为正常(N)组、模型(M)组、复发模型(M+M)组、甲氨蝶呤(MTX)组、固本祛湿化瘀方高、中、低剂量(GQHF-H、M、L)组,每组6只。除N组外... 详细信息

目的探讨固本祛湿化瘀方(GQHF)治疗及延缓银屑病复发的可能作用机制。方法42只Balb/c小鼠按随机数字表法分为正常(N)组、模型(M)组、复发模型(M+M)组、甲氨蝶呤(MTX)组、固本祛湿化瘀方高、中、低剂量(GQHF-H、M、L)组,每组6只。除N组外,其他各给药组从第1天开始灌胃。GQHF-H、M、L组每天给予22.75、11.34、5.69 g/kg;MTX组每天给予MTX 1 mg/kg;N、M组按10 mL/kg容积灌服饮用水,连续15天。第4天,除N、M组外,其余各组小鼠均给予5%咪喹莫特乳膏50 mg建立银屑病样皮损模型;第38天,除N组外,各组给予咪喹莫特建立银屑病复发模型。对银屑病样小鼠进行严重程度指数(PASI)评分、检测各组小鼠脾脏中三磷酸腺苷酶(ATPase)、Ⅱ相代谢酶活性(SULT1 A1、UGT1、GST、SULT1 E1)、水通道蛋白3(AQP3)基因及蛋白表达。结果与M+M组比较,GQHF-H、M、L组和MTX组均显著降低皮损评分(Pk^(+)-ATPase)、钙-镁-三磷酸腺苷酶(Ca^(2+)-Mg^(2+)-ATPase)及总-三磷酸腺苷酶(T-ATPase)活性显著性下降(P0.05);与M+M组比较,GQHF-H组及MTX组脾脏中Na^(+)-k^(+)-ATPase、Ca^(2+)-Mg^(2+)-ATPase、T-ATPase活性显著升高(P<0.05);脾脏中AQP3基因及蛋白的表达显著下降(P<0.01);GQHF-H组在防复发、降低AQP3蛋白表达优于MTX组(P<0.05)。结论GQHF可能是通过升高脾脏ATPase的活性、降低AQP3的表达改善局部内湿环境从而达到治疗及延缓银屑病复发的疗效。

关键词：银屑病复发固本祛湿化瘀方水通道蛋白3 脾脏三磷酸腺苷酶中药复方

Meso-Cenozoic Tectonothermal History of Permian Strata, Southwestern Weibei Uplift: Insights from Thermochronology and Geothermometry

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Acta Geologica Sinica(English Edition) 2019年第6期93卷 1647-1661页

作者： YU Qiang REN Zhanli LI Rongxi TAO ni QI kai JIANG Cheng WANG Baojiang The School of Earth Science and Resources Chang’an UniversityXi’an 710054China key Laboratory of Western China's Mineral Resources and Geological Engineering Ministry of EducationChang'an UniversityXi'an 710054China State key Laboratory of Continental Dynamics Northwest UniversityXi'an 710069China Department of Geology Northwest UniversityXi’an710069China State key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400030China State and Local Joint Engineering Laboratory of Methane Drainage in Complex Coal Gas Seam Chongqing UniversityChongqing 400030China Research Institute of Yanchang Petroleum(Group)Company Limited Xi’anSichuan710075China

This study provides an integrated interpretation for the Mesozoic-Cenozoic tectonothermal evolutionary history of the Permian strata in the Qishan area of the southwestern Weibei Uplift,Ordos *** fission-track and apatite/zircon(U-Th)/He thermochronometry,bitumen reflectance,thermal conductivity of rocks,paleotemperature recovery,and basin modeling were used to restore the Meso-Cenozoic tectonothermal history of the Permian *** Triassic AFT data have a pooled age of^180±7 Ma with one age peak and P(χ2)=86%.The average value of corrected apatite(U-Th)/He age of two Permian sandstones is^168±4 Ma and a zircon(U-Th)/He age from the Cambrian strata is^231±14 *** reflectance and maximum paleotemperature of two Ordovician mudstones are 1.81%,1.57%and^210℃,~196℃*** undergoing a rapid subsidence and increasing temperature in Triassic influenced by intrusive rocks in some areas,the Permian strata experienced four cooling-uplift stages after the time when the maximum paleotemperature reached in late Jurassic:(1)A cooling stage(~163 Ma to^140 Ma)with temperatures ranging from^132℃to^53℃and a cooling rate of^3℃/Ma,an erosion thickness of^1900 m and an uplift rate of^82 m/Ma;(2)A cooling stage(~140 Ma to^52 Ma)with temperatures ranging from^53℃to^47℃and a cooling rate less than^0.1℃/Ma,an erosion thickness of^300 m and an uplift rate of^3 m/Ma;(3)(~52 Ma to^8 Ma)with^47℃to^43℃and^0.1℃/Ma,an erosion thickness of^500 m and an uplift rate of^11 m/Ma;(3)(~8 Ma to present)with^43℃to^20℃and^3℃/Ma,an erosion thickness of^650 m and an uplift rate of^81 m/*** tectonothermal evolutionary history of the Qishan area in Triassic was influenced by the interaction of the Qinling Orogeny and the Weibei Uplift,and the south Qishan area had the earliest uplift-cooling time compared to other parts within the Weibei *** early Eocene at^52 Ma and the late Miocene at^8 Ma,as two significant turning points after which both the rate of uplift and the rat

关键词： low temperature thermochronology fission track (U-Th)/He basin modeling cooling history uplift Ordos Basin

Simulation of Multi-Fluid MHD Flows Based on Adaptive Mesh Refinement Technique

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Journal of Iron and Steel Research International 2012年第S1期19卷 90-94页

作者： PAN Yi ni Ming-Jiu School of Physical Sciences.Graduate University of Chinese Academy of Sciences

Based on an adaptive mesh refinement(AMR)technique,a multi-fluid MHD(magnetohydrodynamics)flow is directly simulated by using volume of fluid(VOF)method to capture the interface and charge conservation(consistent and conservative)scheme to calculate the Lorentz *** of magnetic field on bubble rising in a liquid are conducted,in which the magnetic strength and magnetic directions decide the trajectory and the velocity of rising bubble. The history of kinetic distribution and trajectory is illustrated to describe the effect of the magnetic field on bubble driven flows.

关键词： multiphase flow MHD consistent and conservative scheme AMR VOF

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数字孪生技术在安全工程实践教学中的应用

华北科技学院学报 2024年第2期21卷 118-124页

作者：倪廉钦李树芳高杰袁安营安徽理工大学深部煤矿采动响应与灾害防控国家重点实验室安徽淮南232001 中国安全生产科学研究院北京100012 山西金晖万峰煤矿有限公司山西孝义032300

为了弥补安全工程专业在传统实践教学中的缺陷和不足,进一步提高实践教学质量和学生创新能力,文章以安全工程专业实践教学为基础,论述了数字孪生技术在安全工程实践教学中的发展空间及其应用路径,总结了数字孪生技术在培养学生实践能力... 详细信息

为了弥补安全工程专业在传统实践教学中的缺陷和不足,进一步提高实践教学质量和学生创新能力,文章以安全工程专业实践教学为基础,论述了数字孪生技术在安全工程实践教学中的发展空间及其应用路径,总结了数字孪生技术在培养学生实践能力和创新能力方面的实践案例,并探讨了基于数字孪生技术的安全工程实践教学新模式的构建。研究结果表明:数字孪生技术能够有效弥补安全工程实践教学面临的学科交叉融合、设备资源短缺的薄弱环节。数字孪生技术为突破传统实践教学模式的时空限制,实现数字化、智能化、交互式的知识传递,达到巩固理论教学、培养学生创新能力的良好效果,为优化安全工程实践教学提供了新思路。

关键词：安全工程数字孪生实践教学教学模式人才培养

On-orbit Identification of Time-varying Moment of Inertia for Spacecraft Based on a Recursive Subspace Method

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On-orbit Identification of Time-varying Moment of Inertia fo...