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Open Journal of Forestry 2013年第3期3卷 79-87页

作者： Daniabla Natacha Edwige Thiombiano niéyidouba lamien Ana M.Castro-Euler Barbara Vinceti Dolores Agundez Issaka Joseph Boussim Laboratoire de Biologie et d’Ecologie Vegetales Universite de OuagadougouOuagadougouBurkina Faso Departement de Production Forestiere Institut de l’Environnement et de Recherches AgricolesKoudougouBurkina Faso Instituto Estadual de Floresta Embrapa Amapa Rodovia Juscelino KubitschekMacapaBrazil Bioversity International CGIARHeadquaters:Via Dei TreDenariRomeItaly IniA-CIFOR Dpto Sistemas y Recursos ForestalesCarretera de la CorunaMadridSpain

We examined demand and supply of Food Tree Species (FTS) products in Burkina Faso. The hypotheses were: 1) demand for FTS products by local communities exceeds what can be sustainably extracted, and 2) local communities of the Sudanian zone have access to more diverse FTS products compared to Sahelian zone. Surveys were conducted in 300 households and 360 quadrats in landscapes surrounding 6 villages to determine the diversity, richness and availability of FTS. The results indicate that local communities tend to exploit FTS which are rare to find or absent in the landscape surrounding their village. While the range of FTS largely exploited tends to coincide across the two ecological zones, the diversity and density of the preferred FTS are discordant between the two zones. The results of the present study further support the need for conservation and restoration strategies to sustain the local communities demand for FTS products.

关键词： Human Feeding Food Security Indigenous Knowledge Quantitative Ethnobotany Diversity Threats

Multi-outputs Gaussian process for predicting Burkina Faso COVID-19 spread using correlations from the weather parameters

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Infectious Disease Modelling 2022年第3期7卷 448-462页

作者： Souleymane Zio Bernard lamien Sibiri Tiemounou Yoda Adaman Inoussa Tougri Mohamed Beidari Ouedraogo W.Y.S.Boris Institut du Genie Informatique et Telecom Ecole Polytechnique de OuagadougouOuaga2000Burkina Faso Institut du Genie Industriel et Textile Ecole Polytechnique de OuagadougouOuaga2000Burkina Faso Agence Nationale de la Meteorologie du Burkina Faso ANAMBurkina Faso

The novel coronavirus has affected all regions of the world,but each country has experienced different rates of *** Africa,in particular,infection rates remain low as compared to other parts of the *** heterogeneity in the spread of COVID-19 raises a lot of questions that are still ***,some studies point out that people's mobility,size of gatherings,rate of testing,and weather have a great impact on the COVID-19 *** this work,we first evaluate the correlation between meteorological parameters and COVID-19 cases using Spearman's rank ***,multi-output Gaussian processes(MOGP)are used to predict the daily confirmed COVID-19 cases by exploring its relationships with meteorological *** number of daily reported COVID-19 cases,as well as,weather variables collected from March 9,2020,to October 18,2021,were used in the *** weather variables considered in the analysis are the mean temperature,relative humidity,wind direction,insolation,precipitation,and wind *** predicting model was constructed exploiting the correlation between the data of the daily confirmed COVID-19 cases and data of the weather *** results show that a significant correlation between the daily confirmed COVID-19 cases was found with humidity,wind direction,wind speed,and *** parameters are used to construct the predictive model using the Multi-Output Gaussian process(MOGP).Different combinations of the data of meteorological parameters together with the data of daily reported COVID-19 cases were used to derive different *** found that the best predictor is obtained using the combination of Humidity and *** model is then used to predict the daily confirmed COVID-19 cases knowing the humidity and Insolation.

关键词： Multi-Output Gaussian process COVID-19 Meteorology Correlation Burkina Faso

Edible Wild Fruit Highly Consumed during Food Shortage Period in Togo： State of Knowledge and Conservation Status

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Journal of Life Sciences 2011年第12期5卷 1046-1057页

作者： Abalo Atato Kperkouma Wala Komlan Batawila nieyidouba lamien Koffi Akpagana Laboratoire de Botanique et Ecologie Vegetale Faculte des Sciences Universite de Lome Lome B.P. 1515 Togo Centre Regional de Recherches Environnementales et Agricoles （CRREA） du Centre Koudougou B.P. 10 Burkina Faso

The aim of this work is to inventory Edible Wild Fruit Species （EWFS） highly consumed by local people during food shortage periods in Togo. Ethnobotanical surveys were carried out in four ecological zones （I, II, III ＆ IV） involving a sample of 433 persons from 29 ethnic groups. Semi-structured interview, field observation, group discussions were used to collect data. Food shortage periods were defined using the agricultural calendar of main crops in three ecological zones （I, II, ＆ III）. One hundred and one EWFS belonging to 84 genera and 39 families were inventoried. The three main types of use of the EWFS were direct consumption, condiments and medicines. Twenty among the 101 EWFS recorded were highly consumed during food shortage periods with respectively 15 EWFS in ecological I, 14 in zone 1I and 12 in ecological zone III. All edible fruits consumed during food shortage periods were fresh fruits with abundant pulp. Six fruit species were sold to bring income to local households. These species were those which benefit from conservation measures through their husbandry in agroforestry systems.

关键词： Ethnic group ethnobotany food crisis household uses Togo

Imaging Findings of Children Stroke in Burkina Faso and Etiological Approach

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Open Journal of Radiology 2019年第1期9卷 20-35页

作者： Bénilde Marie Ange Tiemtore-Kambou Prosper Précieux Sagbohan Dominique Bicaba nina-Astrid Nde-Ouedraogo Zakari nikiema Prosper David lamien Adama Gnoumou Ousséï ni Diallo Rabiou Cisse Faculty of Health Sciences Ouaga 1 Pr Joseph Ki-Zerbo University Ouagadougou Burkina Faso Bogodogo Teaching Hospital Ouagadougou Burkina Faso SanouSouroTeaching Hospital Bobo-Dioulasso Burkina Faso Yati Clinic Ouagadougou Burkina Faso Yalgado Ouedraogo Teaching Hospital Ouagadougou Burkina Faso

Introduction: Stroke is defined as a sudden onset focal neurological deficit of presumed vascular origin. Although it is essentially adult and elderly condition, stroke can occur in children. Their diagnosis essentially radiological is based on two main imaging modalities namely Computed Tomography (CT) and/or Magnetic Resonance Imaging (MRI). Our study aimed to study radiological features of stroke in children with an etiologic approach in three health facilities of Ouagadougou. Material and Method: We conducted a retrospective study with descriptive focus during 11-year period from January 1st, 2006 to September 1st, 2017 among children aged between 02 months and 16 years who underwent brain CT scan and/or MRI;in whom diagnosis of stroke had been established in radiology department of Yalgado Ouedraogo Teaching Hospital (CHU-YO), Schiphra Methodist Medical Center (CM Schipphra) and Saint Camille Hospital of Ouagadougou (HOSCO). Results: 40 cases of pediatric stroke were collected including 23 at HOSCO, 15 at CHU-YO and 03 at CM Schipphra. Mean age was 6.45 years, with extremes of 2 months and 192 months. Unilateral contralateral motor weakness was the most common clinical presentation (32.5%) followed by seizures (17.5%). Ischemic stroke dominated in 82.5% of cases. Sino-venous thrombosis was rare and suspected in only one case. Sylvian artery was mostly affected in ischemic stroke for 56.25% of patients. The main etiologic factors found were hematologic (67.50%) then infectious factors (25%). Sickle cell disease was always incriminated in hematologic factor. Homozygous SS patients were the most numerous (82%). Conclusion: Stroke diagnosis in children is based on etiologic investigation, biology with a major role of medical imaging. Sickle cell disease accounts a lot in occurrence of stroke in children in our context.

关键词： Stroke Children Brain CT/MRI Ouagadougou Burkina Faso

Pneumatosis Cystoides Intestinalis Revealed by an Occlusive Syndrome: A Case Report

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Open Journal of Pathology 2023年第4期13卷 210-216页

作者： Franck Auguste Hermann Adémayali Ido Aimé Sosthène Ouédraogo Ibrahim Savadogo Rakiswendé Alexis Ouédraogo Souleymane Ouattara Assita lamien-Sanou Olga Mélanie Lompo Department of Anatomy and Pathological Cytology CHU Tengandogo Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU Bogododgo Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU-R of Ouahigouya Ouahigouya Burkina Faso Histo-Embryo-Cytogenetics Department Bogododgo University Hospital Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU Yalgado Ouédraogo Ouagadougou Burkina Faso

Introduction: Pneumatosis cystoides intestinalis is a rare condition characterized by the presence of gas cysts in the intestinal wall. It can affect different segments of the digestive tract. Male patients over 50 years old seem to be more affected. Classically, one opposes the primary or idiopathic forms and the secondary forms. Case Report: We report the case of a 62-year-old female patient with a history of treated peptic ulcers, who presented with episodes of intermittent atypical abdominal pain that worsened abruptly. The physical examination revealed a surgical abdomen with tenderness to palpation. The patient underwent a jejunal resection. The pathological examination showed macroscopically an intestinal fragment with confluent subserosal and intestinal intraparietal bullae. On opening, the portion containing the bullae was thickened, infarcted, congestive, hemorrhagic, and brittle with narrowing of the intestinal lumen. The bullae are tense and leave serosities. On histological examination, the intestinal wall was very congestive with hemorrhagic patches of suffusion. There were optically empty cystic formations that do not communicate with the digestive lumen. The adjacent mucosa is the site of a moderate subacute inflammatory reaction. Conclusion: Pneumatosis cystoides intestinalis is a rare and usually benign pathology. The symptoms, when they exist, are not specific. Its physiopathology is still very much debated. The prognosis is generally good. The knowledge of this pathology is necessary to avoid invasive explorations and heavy treatment without interest for the patient.

关键词： Cysts Gas Intestinal Pathology

A Case of Cerebral Aspergillosis in a Patient with Chronic Lymphoid Leukemia

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Open Journal of Pathology 2023年第4期13卷 217-223页

作者： Aimé Sosthène Ouédraogo Franck Auguste Hermann Adémayali Ido Ibrahim Savadogo Souleymane Ouattara Rakiswendé Alexis Ouédraogo Assita lamien Sanou Olga Mélanie Lompo Pathological Anatomy Service of Bogodogo Teaching University Hospital Ouagadougou Burkina Faso UFR/SDS Joseph Ki-Zerbo University Ouagadougou Burkina Faso Pathological Anatomy Service of Tingandogo Ouagadougou Burkina Faso Pathological Anatomy Service of Ouahigouya Ouahigouya Burkina Faso Embryology Cytogenetics and Reproductive Biology of Bogodogo Teaching University Hospital Ouagadougou Burkina Faso Pathological Anatomy Service of Yalgado Ouédraogo Ouagadougou Burkina Faso

Cerebral aspergillosis commonly affects immunocompromised hosts, primarily patients on immunosuppressive therapy and those with acquired or immunodeficient states such as AIDS. We report a case of cerebral aspergillosis in a 39-year-old woman with a history of treated chronic lymphocytic leukemia (CLL). Multiple fragments of fixed tissues with formalin were freshly sent to the laboratory. An extemporaneous examination was performed by spreading. The rest of the sample was embedded in paraffin and studied according to the standard histological method with special stains and an immunohistochemical study. A microscopic observation showed abundant clusters of tangled mycelial filaments, Grocott+. At the edge, a nervous tissue was observed remodeled by an abundant inflammatory infiltrate consisting essentially of small lymphocytes and plasma cells. Additional immunohistochemistry was performed using anti-CD20, anti-CD3, anti-CD5, anti-CD23, anti-Bcl2, anti-Ki67, anti-IgD and anti-Kappa and Lamda antibodies. All taken together there were no further founding in support of a secondary localization of CLL. Cerebral aspergillosis is quite rare and often occurs in a context of immunosuppression. This case indicates the importance of a close collaboration between clinicians and pathologists for optimal patient care.

关键词： Cerebral Aspergillosis CLL Grocott Gomori Immunohistochemistry

Radio-Histopathological Aspects of Breast Nodules in a Sub-Saharan African City (Ouagadougou, Burkina Faso)

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Open Journal of Medical Imaging 2018年第3期8卷 89-96页

作者： nina-Astrid Nde Ouedraogo Bénilde Ma Kambou Tiemtore Yobi Alexis Sawadogo Boubakar Ouattara Prosper David lamien Madina Napon Ousséini Diallo Abel Bamouni Claudine Lougue Sorgho Rabiou Cisse Radiology Department of Charles de Gaulle Teaching Paediatric Hospital Ouagadougou Burkina Faso Gynaeco-Obstetric Department of Bogodogo Teaching Hospital Ouagadougou Burkina Faso Radiology Department of Yalgado Ouedraogo Teaching Hospital Ouagadougou Burkina Faso Radiology Department of Charles de Gaulle Teaching Paediatric Hospital Ouagadougou Burkina Faso

Objective: To determine the diagnostic contribution of breast microbiopsy percutaneously under ultrasound guidance. Methodology: Retrospective descriptive study conducted from January 2014 to October 2017. The included patients had an ultrasound-guided breast microbiopsy after a mammographic and ultrasound scan. The variables collected were gender, age, nodule size, ACR classification and anatomopathologic diagnosis. Results: 347 nodules were explored in 313 patients. There were 300 women (95.85%) and 13 men (4.15%), a gender ratio of 23.07. The average age was 44.64 ± 14.18 years. The average size of the nodules was 30.33 ± 19.58 mm. There were 53.89% ACR4 grade nodules, 48% ACR5 nodules and 32.28% ACR3 nodules. 86.49% of ACR3 nodules were benign and 97.92% of ACR5 nodules were actually malignant. Malignant tumors accounted for 50.29% of the nodules and were dominated by invasive breast carcinomas in 98.26%. The average age of patients with malignant tumors was 49.15 ± 11.55 years. 16% of patients with malignancy were aged under 40 years old. Conclusion: The diagnostic yield was satisfactory and there was a good correlation between the rate of malignant and benign lesions and the positive predictive values of malignancy in the literature.

关键词： Breast Microbiopsy Ultrasound Guidance Mammary Nodule Breast Cancer

Anatomo-Clinical Case: Coexistence of Tuberculosis with Axillary Lymph Node Metastasis in Breast Carcinoma

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Open Journal of Pathology 2018年第4期8卷 132-138页

作者： Aimé Sosthène Ouédraogo Hierrhum Aboubacar Bambara Franck Auguste Hermann Ademayali Ido Welbnoaga Norbert Ramdé Rimwaogdo Jeremie Sawadogo Ibrahim Savadogo Souleymane Ouattara Hassami Barry Assita Sanou-lamien Olga Melanie Lompo Pathological Anatomy Unit of Bogodogo District Hospital Ouagadougou Burkina Faso University Department of Research and Training in Health Sciences (UFR/SDS) Ouagadougou Burkina Faso Clinical Oncology and Hematology Unit of Bogodogo District Hospital Ouagadougou Burkina Faso Legal Medicine Unit of Bogodogo District Hospital Ouagadougou Burkina Faso MD MPH CPH Research Institute JPS Health Network Fort Worth TX USA Pathological Anatomy Unit of Ouahigouya Regional Hospital Centre Ouagadougou Burkina Faso Pathological Anatomy Unit of Blaise Compaoré University Hospital Centre Ouagadougou Burkina Faso Yalgado Ouedraogo University Hospital Centre Ouagadougou Burkina Faso

Introduction: The coexistence of tuberculosis with axillary lymph node metastasis in breast carcinoma is uncommon. Observation: We report a case of a patient aged 59 years presenting a painless nodule in the right breast for one year. The scan and mammography revealed a long-axis node of 3 × 2 × 1 cm in the upper outer quadrant of the right breast ranked stage IV by the American College of Radiology (ACR), associated with a set of axillary lymph nodes and the largest one measuring 15 × 15 × 20 millimeters (mm). The breast biopsy helped diagnose a Scarff Bloom Richardson (SBR) grade II non-specific invasive carcinoma, modified by Ellis and Elston. A right mastectomy associated with a lymph node dissection was performed. We noticed a not well defined and whitish 5 mm tumor mass associated with 16 lymph nodes removed. The histological examination confirmed the diagnosis of SBR grade II non-specific invasive carcinoma with invasion of 7 lymph nodes (N+ = 7/16). In 3 metastatic lymph nodes, there were epithelioid and gigantocellular granulomas with full central necrosis. The Ziehl Neelsen staining had highlighted acid-fast bacilli. The tumor was oestrogen and progesteron receptor, without an overexpression of the oncoprotein human epidermal growth factor receptor 2 (HER2), which corresponds to a 0 score and the Ki 67 proliferation index assessed at 10%. The patient was given an anti-tuberculosis treatment combining Rifampicin (H), Isoniazid (I), Pyrazinamid (Z), Ethambutol (E) over 2 months and secondly a combination of Rifampicin and Isoniazid over 4 months (2RHZE/4 RH). The anti-tumor chemotherapy used a protocol combining 3 FAC60+ 3 Docetaxel (F = Fluorouracil®A = Adriblastin®, C = Cyclophosphamid). Conclusion: This coexistence is uncommon, of incidental discovery and necessitates a multidisciplinary care.

关键词： Breast Carcinoma Tuberculosis Lymph Node Histopathology

Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

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American Journal of Molecular Biology 2023年第4期13卷 276-290页

作者： Théodora Mahoukèdè Zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina Marie Angèle Traoré Abdoul Karim Ouattara Florencia Wenkunni Djigma Albert Théophane Yonli Assita Sanou-lamien Olga Mélanie Lompo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. The cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: This study aims to detect the presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. Methods: This descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. Results: The mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV DNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso