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Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： yan Ren yan-ni Cui Hong-Wei Wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all Pnificantly

关键词： Hematopoietic stem cells nicotinamide Concentration Proliferation Differentiation Sirtuin 1

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Feedback enhanced tumor targeting delivery of albumin-based nanomedicine to amplify photodynamic therapy by regulating AMPK signaling and inhibiting GSTs

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Chinese Chemical Letters 2024年第8期35卷 481-485页

作者： Jiaqi Huang Renjiang Kong yanmei Li ni yan Yeyang Wu Ziwen Qiu Zhenming Lu Xiaona Rao Shiying Li Hong Cheng School of Biomedical Engineering&Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical UniversityGuangzhou 510515China School of Pharmaceutical Sciences Guangzhou Medical UniversityGuangzhou 511436China

Oxidative therapies receive a limited antitumor efficiency due to the insufficient reactive oxygen species(ROS)levels at focal sites and the evolvement of antioxidant defense ***,we develop an albumin-based nanomedicine to co-deliver chlorin e6(Ce6)and COH-SR4(CS),which can simultaneously enhance the yield and lethality of intracellular ROS for amplified photodynamic therapy(PDT).In which,CS acts as both an activator of AMP-activated protein kinase(AMPK)and an inhibitor of glutathione S-transferases(GSTs).Benefiting from it,the prepared HSA-Ce6@COH-SR4(HCCS)enables positive feed-back uptake by promoting AMPK phosphorylation,leading to rapid and extensive tumor accumulation of *** a result,HCCS obviously increases the ROS production to elevate intracellular oxidative ***,HCCS can inhibit GSTs to disturb the antioxidant defense system of tumor cells,intensifying the oxidative damage of ***,the PDT of HCCS is significantly strengthened by improving the ROS yield and lethality,which greatly declines the proliferation of breast cancer in *** study may open a window in the development of drug co-delivery system for enhanced oxidative therapy of tumors.

关键词： Human serum albumin Drug delivery Reactive oxygen species Positive feedback uptake Oxidative damage

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Development and validation of a novel non-invasive test for diagnosing nonalcoholic fatty liver disease in Chinese children

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World Journal of Pediatrics 2024年第4期20卷 413-421页

作者： Zhe-Wen Qin Qian-Nan Ren Hong-Xi Zhang Ya-Ru Liu Ke Huang Wei Wu Guan-Ping Dong yan ni Jun-Fen Fu Division of Endocrinology National Clinical Research Center for Child HealthNational Children’s Regional Medical CenterThe Children’s HospitalDepartment of EndocrinologyNational Clinical Research Center for Child HealthNational Children’s Regional Medical CenterThe Children’s HospitalZhejiang University School of MedicineHangzhou310052China

With the exploding prevalence of obesity,many children are at risk of developing nonalcoholic fatty liver *** anthropometric and laboratory parameters,our study aimed to develop a model to quantitatively evaluate liver fat content(LFC)in children with *** A well-characterized cohort of 181 children between 5 and 16 years of age were recruited to the study in the Endocrinology Department as the derivation *** external validation cohort comprised 77 *** assessment of liver fat content was performed using proton magnetic resonance *** and laboratory metrics were measured in all subjects.B-ultrasound examination was carried out in the external validation *** Kruskal-Wallis test,Spearman bivariate correlation analyses,univariable linear regressions and multivariable linear regression were used to build the optimal predictive *** The model was based on indicators including alanine aminotransferase,homeostasis model assessment of insulin resistance,triglycerides,waist circumference and Tanner *** adjusted R^(2)of the model was 0.589,which presented high sensitivity and specificity both in internal[sensitivity of 0.824,specificity of 0.900,area under curve(AUC)of 0.900 with a 95%confidence interval:0.783–1.000]and external validation(sensitivity of 0.918 and specificity of 0.821,AUC of 0.901 with a 95%confidence interval:0.818–0.984).Conclusions Our model based on five clinical indicators was simple,non-invasive,and inexpensive;it had high sensitivity and specificity in predicting LFC in ***,it may be useful for identifying children with obesity who are at risk for developing nonalcoholic fatty liver disease.

关键词： Biochemical markers Children Magnetic resonance spectroscopy Nonalcoholic fatty liver disease Pediatric obesity

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对苏格拉底德性是否可教的误读与澄清

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教育学报 2024年第4期20卷 14-26页

作者：倪艳安徽工程大学马克思主义学院安徽芜湖241000 南京师范大学马克思主义学院南京210023

有不少学者提出苏格拉底的德性可教命题充满悖论,理由是苏格拉底反对从实践(σκητν)获得德性。然而从σκητν的古希腊文原义出发,σκητν所指的并不是“实践”而是“训练”,那么学者们基于“实践”推导出的结论就不可靠。基... 详细信息

有不少学者提出苏格拉底的德性可教命题充满悖论,理由是苏格拉底反对从实践(σκητν)获得德性。然而从σκητν的古希腊文原义出发,σκητν所指的并不是“实践”而是“训练”,那么学者们基于“实践”推导出的结论就不可靠。基于苏格拉底的哲学立场可知,他的结论实际是:真正知识的德性可教,非知识的德性不可教。且结合柏拉图的诸多早中期对话,发现苏格拉底不但没有否认通过实践可以获得德性,而且认可通过训练可以获得德性。苏格拉底的德性教育不只是一种启发式教育,更是引导学生进行理性“训练”的过程。对σκητν的词义追溯和文本分析可知,《美诺》中的德性可教并非矛盾,由此澄清了苏格拉底对理性训练的重视和对实践的真实态度,回归到苏格拉底德性可教的本意。

关键词：苏格拉底德性可教实践训练

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SENP3 Promotes Mantle Cell Lymphoma Development through Regulating Wnt10a Expression

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Current Medical Science 2024年第1期44卷 134-143页

作者： yan-ni MA Yun-ding ZOU Zhi-long LIU Gui-xian WU Yuan-ze ZHOU Cheng-xin LUO Xiang-tao HUANG Ming-ling XIE Shuang-nian XU Xi LI Department of Hematology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Hematology the Third Affiliated Hospital(Daping Hospital)Third Military Medical University(Army Medical University)Chongqing 400042China

Objective SUMO-specific protease 3(SENP3),a member of the SUMO-specific protease family,reverses the SUMOylation of SUMO-2/3 *** of SENP3 has been proven to be involved in the development of various ***,its role in mantle cell lymphoma(MCL),a highly aggressive lymphoma,remains *** study was aimed to elucidate the effect of SENP3 in *** The expression of SENP3 in MCL cells and tissue samples was detected by RT-qPCR,Western blotting or *** cells with stable SENP3 knockdown were constructed using short hairpin *** proliferation was assessed by CCK-8 assay,and cell apoptosis was determined by flow *** sequencing(mRNA-seq)was used to investigate the underlying mechanism of SENP3 knockdown on MCL development.A xenograft nude mouse model was established to evaluate the effect of SENP3 on MCL growth in *** SENP3 was upregulated in MCL patient samples and *** of SENP3 in MCL cells inhibited cell proliferation and promoted cell ***,the canonical Wnt signaling pathway and the expression of Wnt10a were suppressed after SENP3 ***,the growth of MCL cells in vivo was significantly inhibited after SENP3 knockdown in a xenograft nude mouse *** SENP3 participants in the development of MCL and may serve as a therapeutic target for MCL.

关键词： mantle cell lymphoma SENP3 cell proliferation apoptosis

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GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

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World Journal of Stem Cells 2024年第5期16卷 538-550页

作者： yang-Zhou Jiang Lan-Yue Hu Mao-Shan Chen Xiao-Jie Wang Cheng-ning Tan Pei-Pei Xue Teng Yu Xiao-yan He Li-Xin Xiang yan-ni Xiao Xiao-Liang Li Qian Ran Zhong-Jun Li Li Chen Laboratory of Radiation Biology Department of Blood TransfusionLaboratory Medicine CenterThe Second Affiliated HospitalThird Military Medical UniversityChongqing 400037China Hematopoietic Acute Radiation Syndrome Medical and Pharmaceutical Basic Research Innovation Center Ministry of Education of the People’s Republic of ChinaChongqing 400037China

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ***,the positive regulators of ANKRD26 are still *** To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 *** Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family *** to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activi

关键词： Ankyrin repeat domain containing 26 GATA binding protein 2 Thrombocytopenia 2 Transcriptional regulation Myeloid-derived cell lines

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Retinal nerve fiber layer defects and chronic kidney disease:the Kailuan Eye Study

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International Journal of Ophthalmology(English edition) 2024年第9期17卷 1696-1706页

作者： Qian-Qian Wan Jin-Qiong Zhou Li-Jian Fang Ya-Xing Wang Ye-Nan Wang Qian Wang yan-ni yan Xuan yang Shou-Ling Wu Shuo-Hua Chen Jost B Jonas Wen-Bin Wei Beijing Tongren Eye Center Beijing Key Laboratory of Intraocular Tumor Diagnosis and TreatmentBeijing Ophthalmology&Visual Sciences Key LabMedical Artificial Intelligence Research and Verification Key Laboratory of the Ministry of Industry and Information TechnologyBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Ophthalmology the Second Hospital of Anhui Medical UniversityHefei 230000Anhui ProvinceChina Department of Ophthalmology Beijing Liangxiang HospitalCapital Medical UniversityBeijing 102400China Beijing Institute of Ophthalmology Beijing Key Laboratory of Ophthalmology and Visual SciencesBeijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Ophthalmology Xuanwu HospitalCapital Medical UniversityBeijing 100730China Department of Cardiology Kailuan General HospitalTangshan 063000Hebei ProvinceChina Health Care Center Kailuan GroupTangshan 063000Hebei ProvinceChina Department of Ophthalmology Medical Faculty Mannheim of the Ruprecht-Karls-University of HeidelbergHeidelberg 69117Germany

AIM:To investigate whether retinal nerve fiber layer defects(RNFLDs)is a potential risk factor for chronic kidney disease(CKD)in Chinese ***:The Kailuan Eye Study was a populationbased study that included 14440 *** participants underwent detailed assessments,RNFLDs were diagnosed using color fundus ***:Overall,12507 participants[8533 males(68.23%)]had complete systemic examination data and at least one evaluable fundus *** were found in 621 participants[5.0%;95%confidence interval(CI):4.6%-5.34%],and 70 cases of multiple RNFLDs were found(11.27%).After adjusting multiple factors,RNFLDs was significantly associated with CKD severity,the ORs of CKD stage 3,stage 4 and stage 5 were 1.698,4.167,and 9.512,*** RNFLDs were also associated with CKD severity after adjusting multiple factors,the ORs of CKD stage 3 and stage 5 were 4.465 and 11.833 ***,2294 participants had CKD(18.34%,95%CI:17.68%-18.99%).After adjusting for other factors,CKD presence was significantly correlated with the presence of ***:The strongest risk factors for RNFLDs are CKD and ***,RNFLDs can be an ocular feature in patients with *** can help detect systemic diseases,and assessment for RNFLDs should be considered in CKD patients.

关键词： chronic kidney disease retinal nerve fiber layer defects Kailuan Eye Study fundus examination

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Decitabine induces IRF7-mediated immune responses in p53-mutated triple-negative breast cancer:a clinical and translational study

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Frontiers of Medicine 2024年第2期18卷 357-374页

作者： Haoyu Wang Zhengyuan Wang Zheng Wang Xiaoyang Li Yuntong Li ni yan Lili Wu Ying Liang Jiale Wu Huaxin Song Qing Qu Jiahui Huang Chunkang Chang Kunwei Shen Xiaosong Chen Min Lu Department of General Surgery Comprehensive Breast Health CenterRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine(Shanghai)Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Department of Hematology Shanghai Institute of HematologyRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Department of Hematology Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200025China

p53 is mutated in half of cancer ***,no p53-targeting drugs have been ***,we reposition decitabine for triple-negative breast cancer(TNBC),a subtype with frequent p53 mutations and extremely poor *** a retrospective study on tissue microarrays with 132 TNBC cases,DNMT1 overexpression was associated with p53 mutations(P=0.037)and poor overall survival(OS)(P=0.010).In a prospective DEciTabinE and Carboplatin in TNBC(DETECT)trial(NCT03295552),decitabine with carboplatin produced an objective response rate(ORR)of 42%in 12 patients with stage IV *** the 9 trialed patients with available TP53 sequencing results,the 6 patients with p53 mutations had higher ORR(3/6 vs.0/3)and better OS(16.0 vs.4.0 months)than the patients with wild-type *** a mechanistic study,isogenic TNBC cell lines harboring DETECT-derived p53 mutations exhibited higher DNMT1 expression and decitabine sensitivity than the cell line with wild-type *** the DETECT trial,decitabine induced strong immune responses featuring the striking upregulation of the innate immune player IRF7 in the p53-mutated TNBC cell line(upregulation by 16-fold)and the most responsive patient with *** integrative studies reveal the potential of repurposing decitabine for the treatment of p53-mutated TNBC and suggest IRF7 as a potential biomarker for decitabine-based treatments.

关键词： p53 mutation triple-negative breast cancer decitabine DNMT1 IRF7 innate immune response

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Interlayer Magnetic Interaction in the CrI_(3)/CrSe_(2) Heterostructure

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Chinese Physics Letters 2024年第5期41卷 85-89页

作者：王秋皓倪美燕李淑静郑法伟路洪艳张平 School of Physics and Physical Engineering Qufu Normal UniversityQufu 273165China Center for Quantum Physics Key Laboratory of Advanced Optoelectronic Quantum Architecture and MeasurementSchool of PhysicsBeijing Institute of TechnologyBeijing 100081China Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems School of PhysicsBeijing Institute of TechnologyBeijing 100081China College of Mathematics and Physics Beijing University of Chemical TechnologyBeijing 100029China Institute of Applied Physics and Computational Mathematics Beijing 100088China

Based on first-principles calculations, we systematically study the stacking energy and interlayer magnetic interaction of the heterobilayer composed of CrI_(3) and CrSe_(2) monolayers. It is found that the stacking order plays a crucial role in the interlayer magnetic coupling. Among all possible stacking structures, the AA-stacking is the most stable heterostructure, exhibiting interlayer antiferromagnetic interactions. Interestingly, the interlayer magnetic interaction can be effectively tuned by biaxial strain. A 4.3% compressive strain would result in a ferromagnetic interlayer interaction in all stacking orders. These results reveal the magnetic properties of CrI_(3)/CrSe_(2) heterostructure, which is expected to be applied to spintronic devices.

关键词： strain stacking interlayer

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博物馆教育项目的设计、执行和评估——国际博物馆协会教育与文化委员会年度“五佳项目”评选的启示

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东南文化 2024年第1期 146-154页

作者：程艳妮黄瑾汉景帝阳陵博物院陕西西安712038 西安青龙寺遗址保管所陕西西安710043

国际博物馆协会教育与文化委员会每年评选五佳项目,旨在指导和规范教育与文化项目的设计和实施。五佳项目在设计、执行、评估阶段各有其遵循的原则,即设计阶段的目的性、相关性和合理性原则,执行阶段的适宜性原则,评估阶段的科学性和客... 详细信息

国际博物馆协会教育与文化委员会每年评选五佳项目,旨在指导和规范教育与文化项目的设计和实施。五佳项目在设计、执行、评估阶段各有其遵循的原则,即设计阶段的目的性、相关性和合理性原则,执行阶段的适宜性原则,评估阶段的科学性和客观性原则,同时还要阐释在人力物力方面的具体要求。对比中国博物馆的特色项目,中国博物馆在设计阶段存在目的性欠开放、科学相关性考量不足,在执行阶段存在贯彻适宜性专业程度不够深入、博物馆教育方法欠灵活,在评估阶段存在问卷设计欠科学、调查方法较单一、问题分析不足等问题,对此,中国博物馆教育项目设计应合理调整三阶段权重分配比例,遵循可持续发展原则,从而促进博物馆更好发挥教育功能。

关键词：博物馆教育项目国际博协教育与文化委员会五佳项目设计执行评估

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