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Antioxidant and anti-inflammatory activities of Orostachys japonicus

Asian Pacific Journal of Tropical Biomedicine 2020年第11期10卷 516-522页

作者： Eun-Sun Hwang nhuan do thi School of Wellness Industry Convergence Food&NutritionHankyong National University327 Chungang-roAnseong-siGyeonggi-do 17579Korea

Objective: To determine the bioactive compounds of Orostachys japonicus(O. japonicus)(Maxim.) A. Berger extraction by different solvents(70% ethanol or water) and to evaluate the in vitro antioxidant and anti-inflammatory activities. Methods: Total polyphenol, flavonoid, and anthocyanin contents in O. japonicus extract were measured. Antioxidant activities were determined by DPPH, ABTS, and superoxide radical-scavenging ability assays. Anti-inflammatory activities were evaluated by nitric oxide production, tumor necrosis factor-α, and interleukin-6 expression techniques. Results: Extraction with 70% ethanol yielded the highest total polyphenol(60.03 mg/g dry weight) and flavonoid(55.50 mg/g dry weight) contents. The total anthocyanin contents in 70% ethanol and water extracts were 57.25 and 91.71 mg/g dry weight, respectively. The 70% ethanol extract also showed stronger antioxidant activity than the water extract. Antioxidant activity and reducing power increased with the increasing concentration of O. japonicus extract. O. japonicus extract at 0-400 μg/m L did not affect the growth of RAW 264.7 cells, whereas dose-dependent inhibition of nitric oxide, tumor necrosis factor-α, and interleukin-6 production was ***: O. japonicus inhibits oxidative and inflammatory reactions with potentially positive health-related effects.

关键词： Antioxidant inflammatory oxidative

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Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第12期8卷 586-592页

作者： nhuan do thi Eun-Sun Hwang Department of Nutrition and Culinary Science Hankyong National University

Objective: To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9(MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities of the leaf extract. Methods: The effect of aronia leaf extract on cancer prevention was investigated. SK-Hep1 human liver cancer cell line was treated with aronia leaf extract at various concentractions. MTT assay was used to measure cancer cell growth inhibition, and wound migration assay was used for metastasis determination. The expression of MMP-2/-9 was measured at the protein level using zymography and the expression of MMP-2/-9 and MT-1 MMP was examined at the gene level by RT-PCR. Raw 264.7 macrophage cells were stimulated with lipopolysaccharides to induce inflammation, and then the inhibition of inflammation was evaluated by treatment of aronia leaf extract. Expressions of interleukin-6, tumor factor-d. Results: SK-Hep1 cell growth was inhibited毩, and nitric oxide(NO) were also determine in proportion to the concentration of aronia leaf extract. In migration assay, aronia leaf extract showed 61.3%-96.3% wound size inhibtion after treating 50-200 μg/mL of aronia leaf extract for 24 h. At the protein level, the expression of MMP-2 and MMP-9 decreased as the concentration of aronia leaf extract treated with SK-Hep1 cells increased. In addition, the same pattern as in the protein was also observed in the mRNA levels. The expressions of MMP-2 and MMP-9 protein were inhibited by 92.2% and 53.8%, respectively after treatment with 200 μg/mL aronia leaf extract. In addition, Raw 264.7 cells treated with aronia leaf extract did not affect cell survival. There was dose dependent inhibition of interleukine-6, tumor necrosis factor-a and nitric oxide after treating aronia leaf extract in lipopolysaccharides-treated Raw cell. Conclusions: The results show that aronia leaf has anticancer and and antimetastatic properties in SK-Hep1 and Raw 264.7 cells.

关键词： Aronia leaf Cancer Metatasis Lipopolysaccharides-induced Inflammation

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