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Journal of Electromagnetic Analysis and Applications 2019年第4期11卷 41-54页

作者： Vinícius José Oliveira Werneck de Carvalho Luiz Carlos de Carvalho Benyosef Altair souza de assis Departamento de Geofísica Observatório Nacional Rio de Janeiro Brazil Departamento de Matemática Aplicada Universidade Federal Fluminense Niterói Brazil

In general, micropulsation is generated by the free energy within the magnetosphere, which is mainly caused by the magnetosphere-solar wind interactions and/or magnetic field reconnections in the magnetospheric tail. In this article, we studied the types of low-frequency magnetospheric micropulsations Pc5 and Pc6 (1 - 6 mHz), during three magnetic storms, using ground magnetic observations. One of the main motivations of this study was to produce scientific knowledge on the subject in order to fill knowledge gaps in this region, collaborating with existing bibliographies. The data were recorded in a series of six Brazilian stations close to the Dip Equator (dip = 0), with using data from the Ascension Island observatory. Pulse trains were found to occur simultaneously with almost identical waveforms, with significant amplitude increase near magnetic local noon. Amplitude spectra showed a clear similarity in all equatorial seasons. The results obtained were compared with studies performed in regions with similar low latitudes. The possible mechanisms that induced these micropulsations and the peculiarities presented by these data were also discussed in this article.

关键词： Magnetospheric Micropulsations Electrojet Equatorial Alfvén Waves Geomagnetic Storms

Study on enzymes of industrial interest in digestive viscera: Greater amberjack (Seriola dumerili)

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Journal of Coastal Life Medicine 2017年第6期5卷 233-238页

作者： Vagne Melo Oliveira Thiago PajeúNascimento Caio Rodrigo Dias assis Ranilson de souza Bezerra Ana Lúcia Figueiredo Porto Laboratory of Bioactive Product Technology–LABTECBIO Department of Morphology and Animal PhysiologyFederal Rural University of PernambucoRecife-PEBrazil Laboratory of Enzymology–LABENZ Department of BiochemistryFederal University of PernambucoRecife-PEBrazil Laboratory of Immunopathology Keizo Asami Federal University of PernambucoRecife-PEBrazil

Objective:To investigate the presence of industrial enzymes (trypsin and chymotrypsin) as well as collagenolytic and fibrinolytic activities of the neglected processing waste of greater amberjackSeriola dumerili(S. dumerili). Methods: The investigations on trypsin, chymotrypsin, collagenolytic and fibrinolytic activities in residues in digestive viscera were performed. The enzymes trypsin and chymotrypsin were physicochemically characterized (including pH- and thermo-stabilities and sensitivity to metal ions and inhibitors). The kinetic parameters were also determined. Collagenolytic and fibrinolytic activities of the crude extract were also investigated. Results:The crude extract ofS. dumerili showed enzymatic activity of (1.4600 ± 0.0011 and (0.7100 ± 0.0079) IU/mg protein for trypsin and chymotrypsin, respectively. The optimal temperature and pH was 60°C and 9 for trypsin and 40°C and 8 for chymotrypsin. The main inhibitory ions were in the following order: Fe3+> Cu2+> Zn2+> Cd2+> Hg2+ (trypsin) and Hg2+> Fe3+> Zn2+> Cd2+> Mn2+(chymotrypsin). TLCK and benzamidine were the strongest inhibitors for both enzymes. The crude extract also presented collagenolytic activity [(42.44 ± 0.01) IU/mg] and fibrinolytic activity ((26.70 ± 0.05) IU/mL)Conclusions:The crude extract ofS. dumerili showed potential for reuse of its residues and to provide biomolecules of industrial interest like trypsin and to be a source of fibrinolytic enzymes.

关键词： Biomolecules Enzymes Residues Digestive viscera

Conception of a Web Operation System for Processing Petroleum Related Drilling Data: A Focus on Pre-Salt Real-Time Automation and Optimization

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Journal of Software Engineering and Applications 2019年第4期12卷 61-71页

作者： Yuri Soares Pinheiro Lucas Campos Vieira Andreas Nascimento Francisco de assis souza dos Santos Mauro Hugo Mathias Gerhard Thonhauser Asad Elmgerbi Julian Hunt Department of Engineering and Technology Petroleum Engineering Programs Universidade Federal do Espirito Santo (UFES/DETEC) Sao Mateus Brazil Department of Computational and Electronics Universidade Federal do Espirito Santo (UFES/DCEL) Sao Mateus Brazil Department of Petroleum Engineering Chair of Drilling and Completion Engineering Montanuniversit&#228 t Leoben (MUL/DPE/CDC) Leoben Austria International Institute of Applied System Analysis (IIASA/ASA-ENE) Laxenburg Austria Mechanic Department Faculty of Engineering Universidade Estadual Paulista (UNESP/FEG-DME/PRH48-ANP) Guaratingueta Brazil

Petroleum and Natural Gas still represent a considerable share in terms of energy consumption in the current global matrix, so that its exploration/exploitation is present in the market and driving activities in locations of specific complexities, as the ones along unconventional hydrocarbon resources from the Brazilian pre-salt. The daily cost of well drilling under harsh conditions can exceed US $1 million a day, turning any type of downtime or necessary maintenance during the activities to be very costly, moment in which processes optimization starts to be a key factor in costs reduction. Thus, new technologies and methods in terms of automating and optimizing the processes may be of great advantages, having its impact in total related project costs. In this context, the goal of this research is to allow a computation tool supporting achieving a more efficient drilling process, by means of drilling mechanics parameters choosiness aiming rate of penetration (ROP) maximization and mechanic specific energy (MSE) minimization. Conceptually, driven by the pre-operational drilling test curve trends, the proposed system allows it to be performed with less human influences and being updateable automatically, allowing more precision and time reduction by selecting optimum parameters. A Web Operating System (Web OS) was designed and implemented, running in online servers, granting accessibility to it with any device that has a browser and internet connection. It allows processing the drilling parameters supplied and feed into it, issuing outcomes with optimum values in a faster and precise way, allowing reducing operating time.

关键词： Trend Curves Web OS Optimization Pre-Salt Petroleum

Mesenchymal Stem Cells Express Low Levels of Cardiomyogenic Genes and Show Limited Plasticity towards Cardiomyogenic Phenotype

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Journal of Life Sciences 2013年第9期7卷 950-964页

作者： Juliana Lott de Carvalho Danilo Roman Campos Maira souza Oliveira Jader Santos Cruz Nathalia Martins Breyner Dawidson assis Gomes Alfredo Miranda de Goes Department of Biochemistry and Immunology Institute of Biological Sciences Federal University of Minas Gerais Belo Horizonte 30123-970 Minas Gerais Brazil Department of Pharmacology Columbia University Presbyterian Hospital New York 10032 United States Department of Clinic and Surgery College of Veterinary Medicine Federal University of Minas Gerais Belo Horizonte 30123-970 Minas Gerais Brazil Institut National de Recherche Agronomique Paris 75338 France

Mesenchymal stem cell differentiation towards osteogenic, chondrogenic and adipogenic lineages have been extensively described and reproduced in the literature. In contrast, cardiomyogenic differentiation still remains largely controversial. In this study the authors aim to shed new light into this unclear phenomenon and test whether BMMSC （bone marrow mesenchymal stem cells） and ATMSC （adipose tissue derived mesenchymal stem cells） are able to differentiate into functional cardiomyocytes, investigating two differentiation protocols. AT and BMMSC behaved differently when cultured in differentiation media and presented lower levels of proliferation and alkaline phosphatase production, expression of cardiomyocyte-specific transcription factors such as GATA-4, Nkx2-5 and proteins such as ct and 13 Myosin Heavy Chains. Furthermore, MSC started to express higher levels of Connexin-43 and c~ sarcomeric actinin protein. Unfortunately, though, MSC did not present cardiomyocyte-like electrophysiological properties. In order to analyze a possible explanation for such limited plasticity, the authors decided to address the issue using a quantitative approach. Gene expression was quantified by Real time PCR, and, for the first time, the authors show that a possible explanation for limited plasticity of MSC is that even though differentiated cells presented differential gene expression, the levels of key cardiomyogenic genes did not reach expression levels presented by adult cardiomyocytes, nor were maintained along differentiation, reaching peaks at 4 days of stimulation, and decaying thereafter.

关键词： Mesenchymal stem cells cardiomyogenic differentiation limited plasticity in vitro differentiation

Phylogeny of Bacteria from Steelmaking Wastes and Their Acidic Enrichment Cultures

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Advances in Microbiology 2014年第12期4卷 816-828页

作者： mariana P. Reis Flaviane A. Pinheiro Patrícia S. Costa Ana Paula C. Salgado Paulo S. assis Edmar Chartone-souza Andréa M. A. Nascimento Departamento de Biologia Geral Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil Departamento de Engenharia Metalúrgica e de Materiais Escola de Minas Universidade Federal de Ouro Preto Ouro Preto Brazil

Currently, millions of tons of steel are produced worldwide. This has become a serious economic and environmental challenge because the ores used for steel production are nonrenewable resources and the production generates huge amount of waste. In this study, we identified and investigated the ability of bacteria from steelmaking waste with low and high zinc concentration to promote leaching of zinc, when enriched by acidic (pH 2) culture conditions. The bioleaching assays indicated removal of Zn, as in chemical leaching. Bacterial communities from crude and enrichment culture wastes were characterized by the 16S rRNA gene. Phylogenetic analysis of the generated clone libraries revealed predominance of Proteobacteria and Firmicutes. The Actino- bacteria, Bacteroidetes, Cyanobacteria, and Deinococcus-Thermus phyla were also encountered. The clones were most closely related to cultivable heterotrophic bacteria. Different genera were identified including iron redox cycling and leaching bacteria such as Chromobacterium, Aeromonas, Escherichia, Bacillus, and Ochrobactrum. These data add significant new information on bacteria which survive in extremely acidic conditions. They are distantly related to typical acidophiles responsible for the leaching process, which makes them good candidates for future studies on metal bioleaching.

关键词： Bacteria 16S rRNA Diversity Zinc Steelmaking Wastes

Effect of ions on the activity of brain acetylcholinesterase from tropical ifsh

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Journal of Coastal Life Medicine 2015年第7期 505-514页

作者： Caio Rodrigo Dias assis Amanda Guedes Linhares Vagne Melo Oliveira Renata Cristina Penha Franca Juliana Ferreira Santos Elba Verônica Matoso Maciel Carvalho Ranilson souza Bezerra Luiz Bezerra Carvalho Jr Laboratory of Enzymology-LABENZ Biochemistry Department and Laboratory of Immunopathology Keizo AsamiFederal University of PernambucoRecife-PEBrazil Academic Unit of Serra Talhada Federal Rural University of PernambucoSerra Talhada-PEBrazil Laboratory of Glycoproteins Biochemistry DepartmentFederal University of PernambucoRecife-PEBrazil

Objective:To investigate the effect of ions on brain acetylcholinesterase(AChE;EC 3.***.1.7)activities from economic important fish[pirarucu,Arapaima gigas;tambaqui,Colossoma macropomum;cobia,Rachycentron canadum(***)and Nile tilapia,Oreochromis niloticus(***)]comparing with a commercial enzyme from electric eel[Electrophorus electricus(***)].Methods:Thein vitro exposure was performed at concentrations ranging from 0.001 to 10 mmol/L(except for ethylene diamine tetraacetic acid;up to 150 mmol/L).Inhibition kinetics *** *** were also observed through four methods(Michaelis-Menten,Lineweaver-Burk,Dixon and Cornish-Bowden plots)in order to investigate the type of inhibition produced by some ***:Hg^(2+),As^(3+),Cu^(2+),Zn^(2+),Cd^(2+)caused inhibition in all the species under ***^(2+),Mg^(2+)and Mn^(2+)induced slight activation *** enzyme while Pb^(2+),Ba^(2+),Fe^(2+),Li+inhibited the AChE from some of the analyzed *** lowest IC50 and Ki values were estimated *** AChE in presence of Hg^(2+),Pb^(2+),Zn^(2+).Under our experimental conditions,the results *** ***,As^(3+),Cu^(2+),Cd^(2+),Pb^(2+)and Zn^(2+)showed a non-competitive/mixed-type inhibition,while Hg^(2+)inhibited the enzyme in a mixed/competitive-like ***:*** AChE activity was affected by ten of fifteen ions under study showing that this enzyme could undergo interference by these ions when used as pesticide biosensor in environmental *** hindrance would be less relevant for the crude extracts.

关键词： Ions Acetylcholinesterase Biomarkers Fish

Mechanical Circulatory Support in Patients with Sepsis： A Case Series

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Journal of Pharmacy and Pharmacology 2018年第5期6卷 541-546页

作者： Helmgton Jose Brito de souza Henrique Louzan Machado Thiago do Amaral Cavalcante Leonardo Jadyr Santos Rodrigues Alves Diogo assis souza Rafael Ramos Amaral Felipe Bruno Santos da Cunha Marcus Vinicius Nasimento dos Santos Glauco Kalil da Silva Pina Isaac Azevedo Silva Ricardo Borros Corso The Department of Medicine University Center of Brasilia Brasilia 70790-075 Brazil

MCS （mechanical circulatory support） has shown great efficacy in treatment of respiratory mid cardiovascular diseases when conventional therapy is insufficient. The use of ECMO （extracolporeal membrane oxygenation） for the treatment of cardiogenic shock secondary to sepsis is still controversial. Studies have shown that ECMO should be considered as a valuable therapeutic option in cases of refractory cardiovascular dysfunction in severe septic patients, although more studies ale needed to consolidate its efficacy. We report the case of two patients, without previous history of cardiopathy, subjected to temporary MCS, with use of ECMO for the treatment of refractory cardiogenic shock secondary to sepsis.

关键词： ECMO MCS sepsis cardiogenic shock.

Immune response to Helicobacter pylori infection and gastric cancer development

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World Journal of Meta-Analysis 2021年第3期9卷 257-276页

作者： Breno Bittencourt de Brito Fabian Fellipe Bueno Lemos Caroline da Mota Carneiro Andressa Santos Viana Nilo Manoel Pereira Vieira Barreto Gabriela Alves de souza assis Barbara Dicarlo Costa Braga maria Luísa Cordeiro Santos Filipe Antônio França da Silva Hanna Santos Marques Natália Oliveira e Silva Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45031900BahiaBrazil

Gastric adenocarcinoma is a global health concern,and Helicobacter pylori(***)infection is the main risk factor for its *** note,the immune response against the pathogen seems to be a determining factor for gastric oncogenesis,and increasing evidence have emphasized several host and bacterium factors that probably influence in this *** development of an inflammatory process against *** involves a wide range of mechanisms such as the activation of pattern recognition receptors and intracellular pathways resulting in the production of proinflammatory cytokines by gastric epithelial *** process culminates in the establishment of distinct immune response profiles that result from the cytokine-induced differentiation of T naïve cells into specific T helper *** released from each type of T helper cell orchestrate the immune system and interfere in the development of gastric cancer in idiosyncratic ***,variants in genes such as single nucleotide polymorphisms have been associated with variable predispositions for the occurrence of gastric malignancy because they influence both the intensity of gene expression and the affinity of the resultant molecule with its *** addition,various repercussions related to some *** virulence factors seem to substantially influence the host immune response against the infection,and many of them have been associated with gastric tumorigenesis.

关键词： Gastric cancer Helicobacter pylori Immune response Virulence factors Polymorphisms

Tropical Rainfall Measuring Mission Performance in the Measurement of Precipitation for the MuriaéSub-basin

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Journal of Civil Engineering and Architecture 2020年第6期14卷 333-338页

作者： Renata Locarno Frota Francisco de assis souza Filho Victor Costa Porto Tyhago Aragão Dias DEHA UFCFortalezaCE 60455-900Brazil Department of Hydraulic and Environmental Engineering Federal University of CearáFortalezaCE 60020-181Brazil Instituto Superior de Teologia Aplicada—INTA SobralCE 62050-100Brazil

Correct precipitation data are essential for a hydrological ***,the pluviometric stations provide us quite doubtful *** satellites have explored this ***,the spatial estimates of precipitation can be extremely useful,since they present data of the whole surface,since the pluviometers do not exist in areas of difficult *** objective of this work is to analyze the precipitation data obtained by the rain gauges and the Tropical Rainfall Measuring Mission(TRMM)satellite for the Muriaésub-basin,which belongs to the Paraíba do Sul *** was used Thissen method and free software R for the manipulation of the data and its *** results were satisfactory,showing that the estimates of this satellite can be an alternative source of data.

关键词： Precipitation TRMM satellite

Cryptococcus and Cryptococcosis in Human and Animal Model:An Overview

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Advances in Microbiology 2020年第4期10卷 180-193页

作者： Eriques Goncalves da Silva Diniz Pereira Leite-Jr Margareth Léa da Silva Maia Sandra Marilia de souza Silva Francisco de assis Baroni Marcos Ereno Auler Amanda Latércia Tranches Dias Débora Moreira Rinaldo Ferreira Gandra Regina Teixeira Barbieri Ramos André Gasparetto Elisangela Santana de Oliveira Dantas Valéria Tognato Costa Claudete Rodrigues Paula School of Dentistry University of Sao Paulo(USP)Sao PauloBrazil Specialized Medical Mycology Center Federal University of Mato Grosso(UFMT)CuiabáBrazil Federal University of ABC(UFABC) Santo AndréBrazil Rural Federal University of Rio de Janeiro(UFRRJ) Rio de JaneiroBrazil Faculty of Pharmacy University of the Western Center of Paraná(UNICENTRO)Campus CedetegGuarapuavaBrazil Federal University of Alfenas(UNIFAL) AlfenasBrazil University Hospital State University of West Paraná(UNIOESTE)CascavelBrazil Department of Dentistry State University of Maringa(UEM)MaringáBrazil Institute of Biosciences Federal University of Mato Grosso(UFMT)CuiabáBrazil

Background: Yeasts from the genus Cryptococcus (species C. neoformans and C. gattii) are opportunistic pathogens that afflict humans with low immunity caused by diseases such as AIDS or by the usage of immunosuppressive drugs. It is a fatal disease that has been studied around the whole world and its most fatal form is the brain cryptococcosis. Purpose: The present review describes the disease from pathogen isolation, the various clinical presentations of the disease, the most important virulence factors of yeast in human and animal model and their clinical issues. Methods: On this review, several published studies about the disease are presented. Results: Numerous researches have been done worldwide in order to find a kind of therapy that is more effective against the disease. Amphotericin B, in all forms is still the drug of choice in the treatment of the cryptococcosis. Fluconazole, as well as voriconazole in combination with amphotericin B, is recommended in the cases of treatment failure. Conclusion: This study presented has elucidated a little more about the disease. Further studies should be conducted to find more diagnoses that are accurate as well as more effective treatments for eradicating the disease. In this study, the bibliographic survey makes reference to the world literature;with regard to ecology, taxonomy, mains factors related to virulence, the clinical manifestations, the action of antifungal drugs and histopathological analysis used in an animal model, were the objectives deleterious aspects of this study, thus informing, in a simple way, the importance of this microorganism for research and researchers working with this global disease, called Cryptococcosis.

关键词： Cryptocococcus Cryptococcosis Animal Model Cryptococcus neoformans Cryptococcusgattii Virulence Related Factors