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Enhanced Osteogenesis of Nanosized Cobalt-substituted Hydroxyapatite

Journal of Bionic Engineering 2015年第4期12卷 604-612页

作者： nenad ignjatovic Zorica Aj dukovic Jelena Rajkovic Stevo Najman Dragan Mihailovic Dragan Uskokovic Centre for Fine Particles Processing and Nanotechnologies Institute of Technical Sciences of the Serbian Academy of Science and Arts Knez Mihailova 35/4 11000 Belgrade Serbia University of Nis Faculty of Medicine Department of Prosthodontics Clinic of Stomatology Nis Serbia University of Nis Department of Biology and Ecology Faculty of Science and Mathematics Nis Serbia University of Nis Faculty of Medicine Institute of Pathology Nis Serbia

Hydroxyapatite （HAp） is an extensively studied material with known biocompatible and osteoconductive properties in bone tissue reconstruction. The improvement of the osteogenetic potential of HAp has been tested through modification of its structure, by replacing Ca2＋ ions with Co2＋ ions. In our study, we comparatively analyze the osteogenetic potential of the syn- thesized HAp and Co2＋-substituted HAp （HAp/Co） designed on the nano-scale with the aim of specifically stimulating osteo- genesis in vivo. We present a quantitative study of the microscopic organization and structure of the newly formed tissue in a bone defect after 12 weeks and 24 weeks. A quantitative analysis of the calcium, magnesium and phosphorus content in the defect and its close environment was used to determine the deposition of minerals after bone reconstruction. The defect recon- structed with HAp/Co nanoparticles （Co2＋ content 12 wt%） was filled with a new tissue matrix composed of dense collagen fibers containing centers of mineralization after 24 weeks. The mineral deposition rate was also higher when the defect was reconstructed with HAp/Co than when it was filled with pttre HAp. A histological analysis confirmed that the alveolar bone, in which osteoporosis-induced defects were repaired using HAp/Co nanoparticles, was recuperated.

关键词： advanced osteogenesis cobalt-substituted hydroxyapatite nanoparticles histomorphometry

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Comparative morphological and crystallographic analysis of copper powders obtained under different electrolysis conditions

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Transactions of Nonferrous Metals Society of China 2019年第6期29卷 1275-1284页

作者： Nebojsa D. NIKOLIC Ljiljana AVRAMOVIC Evica R. IVANOVIC Vesna M. MAKSIMOVIC Zvezdana BASCAREVIC nenad ignjatovic ICTM-Department of Electrochemistry University of Belgrade Njego.eva 12 Belgrade Serbia Mining and Metallurgy Institute Zeleni Bulevar 35 Bor Serbia Faculty of Agriculture University of Belgrade Nemanjina 6 Belgrade-Zemun Serbia Vinca Institute of Nuclear Sciences University of Belgrade Belgrade Serbia Institute for Multidisciplinary Research University of Belgrade Kneza Viseslava 1a Belgrade Serbia Institute of Technical Sciences of the Serbian Academy of Science and Arts Knez Mihailova 35/IV Belgrade Serbia

Production of copper powders by the potentiostatic electrolysis under different hydrogen evolution conditions was investigated. Copper powders were characterized by the scanning electron microscope(SEM),X-ray diffraction(XRD),particle size distribution(PSD), and by the determination of the specific surface area(SSA) of the formed powders. Depending on quantity of hydrogen generated during electrolysis, the two types of particles were formed: dendrites and cauliflower-like particles. The dendrites were formed without, while cauliflower-like particles with the quantity of evolved hydrogen enough to achieve strong effect on hydrodynamic conditions in the near-electrode layer. Although macro structure of the particles was very different, they showed similar micro structure. Namely, both types of the particles consisted of smalal agglomerates of approximately spherical Cu grains at the micro level. The existence of the spherical morphology was just responsible for random orientation of Cu crystallites in both types of particles. The SSA of cauliflower-like particles was more than two times larger than that of the dendrites, while their size was considerably smaller than that of the dendritic particles. In this way, the useful benefit of Cu powder formation in the conditions of vigorous hydrogen evolution is shown.

关键词： electrolysis copper particles specific surface area(SSA) particle size distribution(PSD)

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