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<i>At</i>L1 a Non-LTR Retrotrasposon Fragment in the Genome of <i>Arabidopsis thaliana </i>with Homology to Plants and Animals

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American Journal of Plant Sciences 2013年第4期4卷 806-816页

作者： Giovanna Visioli Elena Maestri Eugenia Polverini Angelo Pavesi nelson marmiroli Department of Life Sciences University of Parma Parma Italy Department of Physics and Earth Sciences “Macedonio Melloni” University of Parma Parma Italy

We report the isolation of AtL1, a 249 bp non-LTR retrotransposon fragment from Arabidopsis thaliana by fingerprinting mRNAs extracted from A. thaliana plants, ecotype Columbia, in different heat stress conditions. Southern blot and PCR analysis suggested that AtL1 occurs as a single- or low-copy insert in the genome of A. thaliana ecotype Columbia. The presence of AtL1 in the genome of different Arabidopsis ecotypes was confirmed by PCR amplification and sequencing thus excluding all possible contamination. A preliminary scan of the AtL1 nucleotide sequence against the EMBL and NCBI databases revealed a high degree of similarity to a group of LINE type L1 retrotransposons of mammals and with a cDNA sequence of Artemisia annua. A phylogenetic analysis of LINE elements from animals and plants placed AtL1 and A. annua sequences in close proximity to some mammalian sequences but distant from the other plants LINE elements including those from Arabidopsis.

关键词： Arabidopsis Heat Shock Plant Retrotransposons LINE Retrotransposons Horizontal Transposon Transfer (HTT)

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In Vivo Phenotyping for the Early Detection of Drought Stress in Tomato

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Plant Phenomics 2019年第1期1卷 142-151页

作者： Michela Janni Nicola Coppede Manuele Bettelli Nunzio Briglia Angelo Petrozza Stephan Summerer Filippo Vurro Donatella Danzi Francesco Cellini nelson marmiroli Domenico Pignone Salvatore Iannotta Andrea Zappettini Institute of Materials for Electronics and Magnetism(IMEM) National Research Council(CNR)Parco Area delle Scienze 37/A43124 ParmaItaly Institute of Bioscience and Bioresources(IBBR) National Research Council(CNR)Via Amendola 165/A70126 BariItaly Universitàdegli Studi della Basilicata Dipartimento delle Culture Europee e del Mediterraneo:ArchitetturaAmbientePatrimoni Culturali(DICEM)Via S.RoccoI-75100 MateraItaly ALSIA Centro Ricerche Metapontum Agrobios s.s.Jonica 106km 4482MetapontoMT 75010It aly 5Department of Chemistry Life Sciences and Environmental SustainabilityUniversity of ParmaParco Area delle Scienze11/A43124 ParmaItaly

Drought stress imposes a major constraint over a crop yield and can be expected to grow in importance if the climate change predicted comes *** methods are needed to facilitate crop management via the prompt detection of the onset of ***,we report the use of an in vivo OECT(organic electrochemical transistor)sensor,termed as bioristor,in the context of the drought response of the tomato *** device was integrated within the plant’s stem,thereby allowing for the continuous monitoring of the plant’s physiological status throughout its life *** was able to detect changes of ion concentration in the sap upon drought,in particular,those dissolved and transported through the transpiration stream,thus efficiently detecting the occurrence of drought stress immediately after the priming of the defence *** bioristor’s acquired data were coupled with those obtained in a high-throughput phenotyping platform revealing the extreme complementarity of these methods to investigate the mechanisms triggered by the plant during the drought stress event.

关键词： drought thereby prompt

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