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Site-Specific Conjugation of Cell Wall Polyrhamnose to Protein SpyAD Envisioning a Safe Universal Group A Streptococcal Vaccine

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Infectious Microbes & Diseases 2021年第2期3卷 87-100页

作者： Nina J.Gao Satoshi Uchiyama Lucy Pill Samira Dahesh Joshua Olson Leslie Bautista Shilpa Maroju Aym Berges Janet ZLiu Raymond HZurich Nina Mvan Sorge Jeff Fairman neeraj kapoor Victor Nizet Division of Host-Microbe Systems and Therapeutics Department of PediatricsUC San DiegoLa JollaCaliforniaUSA Vaxcyte Inc.Foster CityCaliforniaUSA Department of Medical Microbiology and Infection Prevention Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Netherlands Reference Laboratory for Bacterial Meningitis Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Skaggs School of Pharmacy and Pharmaceutical Sciences UC San DiegoLa JollaCaliforniaUSA

Development of an effective vaccine against the leading human bacterial pathogen group A Streptococcus(GAS)is a public health *** species defining group A cell wall carbohydrate(GAC,Lancefield antigen)can be engineered to remove its immunodominant N-acetylglucosamine(GlcNAc)side chain,implicated in provoking autoimmune cross-reactivity in rheumatic heart disease,leaving its polyrhamnose core(GACPR).Here we generate a novel protein conjugate of the GACPR and test the utility of this conjugate antigen in active *** of conjugation to a standard carrier protein,we selected SpyAD,a highly conserved GAS surface protein containing both B-cell and T-cell epitopes relevant to the bacterium that itself shows promise as a vaccine *** was synthesized using the XpressTM cell-free protein expression system,incorporating a non-natural amino acid to which GACpr was conjugated by site-specific click chemistry to yield high molecular mass SpyAD-GACPR conjugates and avoid disruption of important T-cell and B-cell immunological *** conjugated SpyAD-GACPR elicited antibodies that bound the surface of multiple GAS strains of diverse M types and promoted opsonophagocytic killing by human *** immunization of mice with a multivalent vaccine consisting of SpyAD-GACPR,together with candidate vaccine antigens streptolysin O and C5a peptidase,protected against GAS challenge in a systemic infection model and localized skin infection model,without evidence of cross reactivity to human heart or brain tissue *** general approach may allow GAC to be safely and effectively included in future GAS subunit vaccine formulations with the goal of broad protection without autoreactivity.

关键词： group A Streptococcus Streptococcus pyogenes group A carbohydrate Lancefield antigen conjugate vaccine XpressCFTM non-natural amino acids SpyAD Introduction

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Design and evaluation of Swift routing for payment channel network

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Blockchain(Research and Applications) 2024年第2期5卷 62-72页

作者： neeraj Sharma Kalpesh kapoor V.Anirudh Department of Computer Science and Engineering Indian Institute of Technology GuwahatiGuwahati 781039AssamIndia Department of Mathematics Indian Institute of Technology GuwahatiGuwahati 781039AssamIndia

Payment Channel Networks(PCNs)are a promising alternative to improve the scalability of a blockchain network.A PCN employs off-chain micropayment channels that do not need a global block confirmation procedure,thereby sacrificing the ability to confirm transactions *** uses a routing algorithm to identify a path between two users who do not have a direct channel between them to settle a *** performance of most of the existing centralized path-finding algorithms does not scale with network *** rapid growth of Bitcoin PCN necessitates considering distributed ***,the existing decentralized algorithms suffer from resource *** present a decentralized routing algorithm,Swift,focusing on fee *** concept of a secret path is used to reduce the path length between a sender and a receiver to optimize the ***,we reduce a network structure into combinations of cycles to theoretically study fee optimization with changes in cloud *** secret path also helps in edge load sharing,which results in an improvement of *** routing achieves up to 21%and 63%in fee and throughput optimization,*** results from the simulations follow the trends identified in the theoretical analysis.

关键词： Blockchain Payment channel networks Bitcoin Lightning network Routing protocols Layer 2 protocols

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