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Genetic linkage mapping and QTL identification for salinity tolerance in Indian mustard(Brassica juncea *** and Coss.)using SSR markers

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Oil Crop Science-中国油料作物学报英文版 2023年第3期8卷 191-205页

作者： Rekha Patel Sumit Jangra Ram Avtar neelam R.yadav Ram C.yadav Department of Molecular Biology Biotechnology&BioinformaticsCCS Haryana Agricultural UniversityHisar-125004India Department of Genetics and Plant Breeding CCS Haryana Agricultural UniversityHisar125004India

Soil salinity is one of the major environmental constraints that limits crop yield and nearly 7%of the total area worldwide is affected by ***-induced oxidative stress causes membrane damage during germination and seedling *** mustard is a major oilseed crop in India and its production and productivity are severely affected by salt *** Brassica cultivars for salinity tolerance by conventional means is very difficult and ***,understanding the molecular components associated with salt tolerance is needed to facilitate breeding for salt tolerance in *** this investigation,quantitative trait loci(QTLs)associated with salt tolerance were identified using F_(2:3)mapping population developed from a cross between CS52(salinity tolerant)and RH30(salinity sensitive).Parents and F_(2:3)were evaluated under controlled and salinity stress conditions for 14 morpho-physiological traits for two consecutive generations(F2 and F_(2:3)),explaining proportion of the phenotypic variance under control *** sequence repeat(SSR)markers were used for mapping studies.A genetic linkage map based on 42 simple sequence repeats(SSRs)markers was constructed covering 2298.5 cM(Haldane)to identify the loci associated with salt tolerance in Brassica ***-one SSRs showing polymorphism in the parents(CS52 and RH30)were mapped on 8 linkage groups(C1–C8).One marker(nga 129)did not map to any of the linkage group and was excluded from *** group 5(C5;317.9 cM)was longest and linkage group 1(C1,255.0 cM)was ***,we identified 15 QTLs controlling 8 traits using F_(2:3)*** QTLs explained 12.44–60.63%of the phenotypic variation with a LOD score range of 3.62–*** of these QTLs,QMI4.1 related to membrane injury showed 51.28%phenotypic variance with a LOD score of *** QBYP8.1 related to biological yield per plant showed 60.63%phenotypic variance at a LOD score of *** highest LO

关键词： Brassica juncea Salt stress tolerance SSR markers Linkage mapping QTL identification

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High-Throughput Phenotyping:A Platform to Accelerate Crop Improvement

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Phenomics 2021年第2期1卷 31-53页

作者： Sumit Jangra Vrantika Chaudhary Ram C.yadav neelam R.yadav Department of Molecular Biology Biotechnologyand BioinformaticsCCS Haryana Agricultural UniversityHisar 125004India

Development of high-throughput phenotyping technologies has progressed considerably in the last 10 *** technologies provide precise measurements of desired traits among thousands of field-grown plants under diversified environments;this is a critical step towards selection of better performing lines as to yield,disease resistance,and stress tolerance to accelerate crop improvement ***-throughput phenotyping techniques and platforms help unrave-ling the genetic basis of complex traits associated with plant growth and development and targeted *** review focuses on the advancements in technologies involved in high-throughput,field-based,aerial,and unmanned *** of user-friendly data management tools and softwares to better understand phenotyping will increase the use of field-based high-throughput techniques,which have potential to revolutionize breeding strategies and meet the future needs of stakeholders.

关键词： High-throughput phenotyping Imaging Technologies Platform Crop improvement Biotic and abiotic stress

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Biodiversity,current developments and potential biotechnological applications of phosphorus-solubilizing and-mobilizing microbes:A review

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Pedosphere 2021年第1期31卷 43-75页

作者： Divjot KOUR Kusam Lata RANA Tanvir KAUR neelam yadav Ajar Nath yadav Manish KUMAR Vinod KUMAR Harcharan Singh DHALIWAL Anil Kumar SAXENA Department of Biotechnology Dr.Khem Singh Gill Akal College of AgricultureEternal UniversityBaru SahibSirmour 173101(India Gopi Nath P.G.College Veer Bahadur Singh Purvanchal UniversityGhazipur 275201(India Amity Institute of Biotechnology Amity UniversityGwalior 474005(India Biochemistry Forage SectionCollege of AgricultureCCS Agricultural UniversityHaryana 125004India ICAR-National Bureau of Agriculturally Important Microorganisms Kusmaur 275103(India)

As one of the most important and essential macronutrients next to nitrogen,phosphorus(P)is important for plant development,but it is the least mobile nutrient element in plant and ***,P is mined from geological sediments and added to agricultural soils so as to meet the critical requirements of crop plants for agronomic *** exists in soil in both organic and inorganic *** various inorganic forms of the element in soil are salts with calcium,iron,and aluminum,whereas the organic forms come from decaying vegetation and microbial *** is a huge diversity of plant microbiomes(epiphytic,endophytic,and rhizospheric)and soil microbiomes that have the capability to solubilize the insoluble P and make it available to *** main mechanism for the solubilization of inorganic P is by the production of organic acids,which lowers soil pH,or by the production of acid and alkaline phosphatases,which causes the mineralization of organic *** P-solubilizing and-mobilizing microorganisms belong to all three domains,comprising archaea,bacteria,and *** strains belonging to the genera Arthrobacter,Bacillus,Burkholderia,Natrinema,Pseudomonas,Rhizobium,and Serratia have been reported as efficient and potential P *** use of P solubilizers,alone or in combination with other plant growth-promoting microbes as an eco-friendly microbial consortium,could increase the P uptake of crops,increasing their yields for agricultural and environmental sustainability.

关键词： crop improvement gene expression microbial diversity organic acid production P mineralization P solubilizer phosphatase production plant growth-promoting traits

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Is the speed of adjusting to environmental change condition dependent?An experiment with house mice(Mus musculus)

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Current Zoology 2024年第3期70卷 350-360页

作者： Karem Lopez-Hervas neelam Porwal Mathilde Delacoux Alexandros Vezyrakis Anja Guenther RG Behavioural Ecology of Individual Differences Max Planck Institute for Evolutionary Biology24306 PlönGermany Department of Evolutionary Biology Faculty of BiologyAdam Mickiewicz UniversityWieniawskiego 161-712 PoznańPoland Department for Collective Behaviour Max Planck Institute of Animal Behaviour78464 ConstanceGermany Centre for the Advanced Study of Collective Behaviour University of Konstanz78464 ConstanceGermany Animal Ecology Institute for Biochemistry and BiologyUniversity of Potsdam14469 PotsdamGermany

Environmental conditions change constantly either by anthropogenic perturbation or naturally across space and ***,a change in behavior is the frst response to changing *** fexibility can potentially improve an organism’s chances to survive and ***,we lack an understanding on the time-scale such behavioral adjustments need,how they actually affect reproduction and survival and whether behavioral adjustments are suffcient in keeping up with changing *** used house mice(Mus musculus)to test whether personality and life-history traits can adjust to an experimentally induced food-switch fexibly in adulthood or by intergenerational plasticity,that is,adjustments only becoming visible in the offspring *** lived in 6 experimental populations of semi-natural environments either on high or standard quality food for 4 *** showed previously that high-quality food induced better conditions and a less risk-prone ***,we tested whether the speed and/or magnitude of adjustment shows condition-dependency and whether adjustments incur ftness ***-history but not personality traits reacted fexibly to a food-switch,primarily by a direct reduction of reproduction and sloweddown *** whose parents received a food-switch developed a more active stress-coping personality and gained weight at a slower rate compared with their respective ***,the modulation of most traits was condition-dependent,with animals previously fed with high-quality food showing stronger *** study highlights that life-history and personality traits adjust at different speed toward environmental change,thus,highlighting the importance of the environment and the mode of response for evolutionary models.

关键词： animal personality context dependence environmental change Match-mismatch non-genetic inheritance phenotypic flexibility phenotypic plasticity

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利用体外培养选育芥菜型油菜和白芥种间杂种改良抗黑斑病的性状(英文)

利用体外培养选育芥菜型油菜和白芥种间杂种改良抗黑斑病的性状(...

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第十二届国际油菜大会

作者： R.C.yadav Dhiraj Singh neelam yadav Somveer Nimbal Naveen Chandra Omender Sangwan Amit Singh Harbinder Singh Deptt.of Biotechnology and Molecular Biology CCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） Deptt.of Biotechnology and Molecular Biology CCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） Oilseeds section Deptt.of Plant BreedingCCS Haryana Agricultural UniversityHisar-125004 （India） National Research Centre on Rapeseed-Mustard SewarBharatpur 321303 （India）

白芥（Brassica alba）是黑斑病抗性的重要资源,能为印度芥菜型油菜转移该性状提供有价值的资源材料。遗憾的是,传统的方法不能成功获得种间杂种,而种间杂种对改良印度芥菜型油菜的黑斑病抗性至关重要。本研究通过体外培养来挽救种间杂... 详细信息

白芥（Brassica alba）是黑斑病抗性的重要资源,能为印度芥菜型油菜转移该性状提供有价值的资源材料。遗憾的是,传统的方法不能成功获得种间杂种,而种间杂种对改良印度芥菜型油菜的黑斑病抗性至关重要。本研究通过体外培养来挽救种间杂种。将两个优良的芥菜型油菜栽培品种即Varuna和RH0345与白芥进行种间杂交,于授粉后20天取含有正在发育的种子的未成熟角果,经表面消毒后将正在发育的种子培养在经改良的Murashige and Skoog（1962）培养基中。然后将杂种幼苗培养在两种MS培养基中,一种不含生长调节剂而添加500mg/l酪蛋白水解物,另一种添加2.5mg/l激动素。结果发现两种培养基都非常适合挽救种间杂种,在培养中植株表现出双亲的形态特征。这些植株通过茎段培养保存,下一季节种植在田间进行鉴定,筛选对黑斑病抗性和其它农艺性状都好的杂种。

关键词： Indian mustard (Brassica juncea Czern & Coss.) White mustard (Brassica alba) Interspecific hybridization In vitro culture Alternaria blight

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Management of proximal tibial stress fracture associated with advanced knee osteoarthritis:A systematic review

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Chinese Journal of Traumatology 2024年第3期27卷 147-152页

作者： Mukesh Kumar Saini Mahendra Singh Devendra Singh Prem Manohar Seervi Pera Jayavardhan Reddy neelam Ramana Reddy Department of Orthopaedics Dr SN Medical CollegeJodhpur342011India Department of Orthopaedics Star HospitalsB Block Banjara HillsHyderabad500034India

Purpose:Tibial stress fracture associated with knee osteoarthritis is an unusual and difficult clinical *** is no clear existing treatment guideline for this uncommon clinical *** aim of this study is to review the impact of various treatment options for patients with advanced knee osteoarthritis associated with proximal tibial stress ***:The study was performed using the databases of PubMed and *** index for non-randomized studies score was used to evaluate the included studies’*** concluded data included the treatment approach,reported outcome measure,and time to fracture *** literature search was started in December 2021 and accomplished in January 2022.A narrative description of the different methods and comparison of their results were ***:Out of total assessed 69 studies,9 studies were included in our *** commonest treatment approach used was total knee arthroplasty by long tibial stem *** mean preoperative knee society score and knee functional score were 30.62 and 23.17,*** mean postoperative knee society knee score was 86.87,while the functional score was *** average reported time to achieve fracture union was 4 months(a range of 2.07-5.50 months).Conclusion:The optimal clinical outcome for treating either acute or mobile tibial stress fracture in patients with advanced knee osteoarthritis can be achieved with long stem total knee ***,due to heterogeneity of data,comparison of different treatment options for chronic proximal tibial stress fracture mal-union/non-union coexisting with knee osteoarthritic and such inferences need to be judged cautiously.

关键词： Tibial stress fracture Knee osteoarthritis Total knee arthroplasty

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Geospatial Analysis of Urban Heat Island Effects and Tree Equity

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Open Journal of Forestry 2024年第1期14卷 1-18页

作者： Jillian Gorrell Sharon R. Jean-Philippe Paul D. Ries Jennifer K. Richards neelam C. Poudyal Rochelle Butler School of Natural Resources University of Tennessee 203 Third Creek Building Knoxville Tennessee USA

In recent decades, Urban Heat Island Effects have become more pronounced and more widely examined. Despite great technological advances, our current societies still experience great spatial disparity in urban forest access. Urban Heat Island Effects are measurable phenomenon that are being experienced by the world’s most urbanized areas, including increased summer high temperatures and lower evapotranspiration from having impervious surfaces instead of vegetation and trees. Tree canopy cover is our natural mitigation tool that absorbs sunlight for photosynthesis, protects humans from incoming radiation, and releases cooling moisture into the air. Unfortunately, urban areas typically have low levels of vegetation. Vulnerable urban communities are lower-income areas of inner cities with less access to heat protection like air conditioners. This study uses mean evapotranspiration levels to assess the variability of urban heat island effects across the state of Tennessee. Results show that increased developed land surface cover in Tennessee creates measurable changes in atmospheric evapotranspiration. As a result, the mean evapotranspiration levels in areas with less tree vegetation are significantly lower than the surrounding forested areas. Central areas of urban cities in Tennessee had lower mean evapotranspiration recordings than surrounding areas with less development. This work demonstrates the need for increased tree canopy coverage.

关键词： Spatial Analysis Land Cover Urban Heat Island Effect (UHIE) Evapotranspiration Tree Canopy Impervious Surface GIS Prediction Model GIS Machine Learning

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Microwave gel combustion synthesis and sinterability of Nd:GGG nanopowders

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Journal of Rare Earths 2014年第6期32卷 521-525页

作者： Kiranmala Laishram Rekha Mann neelam Malhan Laser Materials Division Laser Science and Technology Centre DRDO

Neodymium doped gadolinium gallium oxide （Nd：GGG） nanopowders synthesized by microwave gel combustion using alanine as a fuel was reported. Metal nitrates solution with alanine fuel was combusted in microwave to give precursor. The micro-wave precursor powder was calcined at different temperatures from 800 to 1100 ℃. Phase pure Nd：GGG formation took place at 800 to 1100 ℃ as observed by X-ray diffraction （XRD） and Fourier transform infra-red （FTIR） spectroscopy. However particle size in-creased with calcinations temperature from 25 nm at 800 ℃ to 200 nm at 1100 ℃.Nd：GGG nanopowder obtained at different calci-nation temperatures were compacted and sintered at 1550 ℃ for 3 h in air. Most densified ceramic was obtained from Nd：GGG nanopowder calcined at 1100 ℃. Microstructure as observed from scanning electron microscopy （SEM） showed that the most densi-fied ceramic, obtained from nanopowder calcined at a higher calcination temperature, had a more uniform grain-size distribution, fewer pores and greater densification. XRD of sintered sample showed retention of phase purity.

关键词： Nd:GGG microwave gel combustion calcinations densification rare earths

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Current testing strategies for hepatitis C virus infection in blood donors and the way forward

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World Journal of Gastroenterology 2014年第11期20卷 2948-2954页

作者： neelam Marwaha Suchet Sachdev Department of Transfusion Medicine Post Graduate Institute of Medical Education and ResearchChandigarh 160012India

Screening tests for blood donations are based upon sensitivity, cost-effectiveness and their suitability for high-throughput testing. Enzyme immunoassay (EIAs) for hepatitis C virus (HCV) antibodies were the initial screening tests introduced. The ”first generation“ antibody EIAs detected seroconversion after unduly long infectious window period. Improved HCV antibody assays still had an infectious window period around 66 d. HCV core antigen EIAs shortened the window period considerably, but high costs did not lead to widespread acceptance. A fourth-generation HCV antigen and antibody assay (combination EIA) is more convenient as two infectious markers of HCV are detected in the same assay. Molecular testing for HCV-RNA utilizing nucleic acid amplification technology (NAT) is the most sensitive assay and shortens the window period to only 4 d. Implementation of NAT in many developed countries around the world has resulted in dramatic reductions in transfusion transmissible HCV and relative risk is now < 1 per million donations. However, HCV serology still continues to be retained as some donations are serology positive but NAT negative. In resource constrained countries HCV screening is highly variable, depending upon infrastructure, trained manpower and financial resource. Rapid tests which do not require instrumentation and are simple to perform are used in many small and remotely located blood centres. The sensitivity as compared to EIAs is less and wherever feasible HCV antibody EIAs are most frequently used screening assays. Efforts have been made to implement combined antigen-antibody assays and even NAT in some of these countries.

关键词： Hepatitis C virus Screening tests Blood donors Immunoassays Nucleic acid testing

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Single step low temperature synthesis of gadolinium gallium garnet nanopowders

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Journal of Rare Earths 2012年第6期30卷 545-547页

作者： Rekha Mann Kiranmala Laishram neelam Malhan Laser Materials Division Laser Science and Technology CentreDRDOMetcalfe House Delhi-110054India

Solution combustion synthesis of single-phase gadolinium gallium oxide （Gd3GasO12, GGG） nanopowders, by a fuel mixture approach using urea and glycine at a low temperature of 500 ℃, was being reported for the first time. Based on the fact that urea and glycine are good fuels for gallium oxide and gadolinium oxide synthesis, the fuel mixture composition was obtained, which could lead to direct phase pure cubic Gd3Ga5O12 formation without any subsequent calcination step. Combustion was carried out in furnace pre-heated at 500 ℃. Thermogravimetric analysis （TGA） of combustion product showed negligible mass loss indicating direct formation of GGG powder. Fourier transform infrared （FTIR） spectrum of combusted product showed peak characteristic of GGG in case of mixed fuel. X-ray diffraction （XRD） confirmed formation of phase pure GGG at 500 ℃ in preheated furnace. Very fine, well dispersed nanometric particles of size range of 50-100 nm were obtained, being uniform and close to spherical morphology as observed by transmission electron microscope （TEM）.

关键词： direct synthesis solution combustion GGG mixed fuel calcinations free rare earths

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