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pOLYMERASE CHAIN REACTION IN DETECTION OF HUMAN pApILLOMAVIRUS DNA AND TYpES OF CONDYLOMA ACUMINATA

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中华医学杂志(英文版) 1993年第2期106卷 141-144页

作者：朱文元 Craig Leonardi neal S.penneys Department of Dermatology Nanjing Medical CollegeNanjing 210029 Department of Dermatology and Cutaneotls Surgery University of Miami School of MedicineMiamiFlorida 33101USA Department Of Dermatology and Cutaneous Surgery University of Miami School of MedicineMiamiFlorida 33101USA

Sections from 88 paraffin-embedded tissues identifiedhistopathologically as condyloma acuminata were assayed forthe presence of human papillomavirus (HpV) types 6,11,16,18 and 33 using polymerase chain reaction followed by *** DNA sequences were found in all tissuesof condyloma *** type 6 was present in 71tissues,type 11 in 16,and type 16 in *** man was doublyinfected with HpV types 6 and *** HpV types 18 and 33were *** conclude that condyloma acuminata is mainlyproduced by HpV types 6 and 11.

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Genomic Stability of palmer amaranth plants Derived by Macro-Vegetative propagation

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American Journal of plant Sciences 2014年第21期5卷 3302-3310页

作者： neal Dwaine Teaster Robert Edward Hoagland US Department of Agriculture-Agricultural Research Service Crop Production Systems Research Unit Stoneville USA

qpCR (quantitative polymerase chain reaction) and random amplified polymorphic DNA (RApD) were utilized to investigate genetic stability of palmer amaranth cloned plants over 10 generations. DNA from original parent palmer amaranth plants (grown from seeds) was re-analyzed using qpCR, and confidence levels for determining ΔΔCt (threshold crossing) values were established. ANOVA was used to determine variation (margin of error) of these ΔΔCt values. This margin of error was applied to qpCR analysis of DNA from eight individual parent plants and their descendants (10th generation) so that possible differences in EpSpS (5-enolpyruvylshikimate-3-phosphate synthase) gene copy number could be ascertained. This method (and the associated error) indicated a lack of agreement in ΔΔCt values of DNA from plants of these two generations. qpCR analysis showed that in five out of eight clones, EpSpS gene copy number varied more than the calculated error (p = 0.05). A second technique to monitor genetic stability, RApD was used to determine possible changes in genomic DNA due to extended cloning of these regenerated plants. RApD analysis showed that four out of the eight clones differed when the profiles of the two generations were compared. Results show that qpCR and RApD analysis point to the fact that several palmer amaranth clones experienced changes in genome structure over 10 generations. Although the glyphosate resistance trait was retained, results suggest that during cloning studies, the genetic stability of macro-vegetatively propagated lines should be monitored.

关键词： Amaranthus palmeri EpSpS Gene Copy Number Glyphosate-Resistant Weeds palmer amaranth pigweed qpCR RApD

Zero-waste emission design of sustainable and programmable actuators

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SusMat 2023年第2期3卷 207-221页

作者： Linchao Sun Lixuan Che Ming Li William George neal Xu Leng Yaojia Long Yifeng Jia Yunhu Gao Matteo palma Yao Lu Department of Chemistry School of Physical and Chemical SciencesQueen Mary University of LondonLondonUK State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalianChina Chemistry Research Laboratory Department of ChemistryUniversity of OxfordOxfordUK Department of Engineering University of CambridgeCambridgeUK

Moisture-responsive actuators are widely used as energy-harvesting devices due to their excellent ability to spontaneously and continuously convert external energy into kinetic ***,it remains a challenge to sustainably synthesize moisture-driven ***,we present a sustainable zero-waste emission methodology to prepare soft actuators using carbon nano-powders and biodegradable polymers through a water evaporation *** to the water solubility and recyclability of the matrixes employed here,the entire synthetic process achieves zero-waste *** composite films featured strong figures of merit and capabilities with a 250◦maximum bending angle under 90%relative *** motions and intelligent bionic applications,including walkers,smart switches,robotic arms,flexible excavators,and hand-shaped actuators,were further achieved by modulating the geometry of the *** sustainable method for actuators’fabrication has great potential in large-scale productions and applications due to its advantages of zero-waste emission manufacturing,excellent recyclability,inherent adaptive integration,and low cost.

关键词： programmable motions recyclability sustainable actuators zero-waste emission

国际分工与美中贸易逆差：以iphone为例

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金融研究 2011年第3期 198-206页

作者：邢予青 neal Detert 亚洲开发银行研究院日本政策研究大学院大学

本文以iphone为例,来阐明即使那些由美国公司发明的高科技产品,也可能不会增加美国出口,反而会扩大美国贸易逆差。2009年中美iphone贸易逆差约为19亿美元。空前的全球化、组织良好的全球生产网络、迅速发展的国际分工以及廉价的运输成本... 详细信息

本文以iphone为例,来阐明即使那些由美国公司发明的高科技产品,也可能不会增加美国出口,反而会扩大美国贸易逆差。2009年中美iphone贸易逆差约为19亿美元。空前的全球化、组织良好的全球生产网络、迅速发展的国际分工以及廉价的运输成本,都可能影响跨国公司如Apple公司的经营决策,而这些经营决策可能直接恶化美国的贸易逆差。全球生产网络与高度专业化的生产过程,明显改变了贸易形态:像中国这样的发展中国家现在出口iphone这样的高科技产品,而像美国这样的工业化国家则进口原本由自己发明的高科技产品。此外,传统贸易统计方法无法识别制造业产品价值的跨国分配,极度夸大了被跨国公司作为出口平台的国家与目标市场国家之间的双边贸易失衡。

关键词： iphone 国际生产链中美贸易失衡

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Biodynamic practices, Eco-label Wines and Millennial Consumers

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Journal of Agricultural Science and Technology(A) 2012年第12期2卷 1364-1372页

作者： Michael McCullough Eivis Qenani neal MacDougall Agribusiness Department California Polytechnic State University San Luis Obispo CA 93407 USA

Abstract： The objective of this study was to provide insights on the motivations behind the decision of biodynamic producers in the United States to shift to biodynamic practices in the vineyard and winemaking process. The second objective of the study was to evaluate the perceptions of the consumers regarding eco-label wines, with a direct focus on the attitudes of millennial generation. Two surveys, one directed to biodynamic producers in the United States and the other to a group of Californian wine consumers were distributed during spring and fall of 2010. Findings from the first survey reveal that biodynamic producers are engaged in biodynamic practices out of socially motivated reasons rather than economic factors. Results from the consumer survey suggest a generally uninformed generation of consumers about eco-label wines and particularly about the practices associated with these products. This lack of information heavily influences their behavioral decisions and attitudes and points out to the critical need for consumer education.

关键词： Eco-label wine biodynamic wine biodynamic practices millennial consumers Chi-square tests total word count:4 555.

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函数式编程思维

Work Instability among RNs with Sensory Disabilities

作者： (美)neal Ford

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Work Instability among RNs with Sensory Disabilities

第一届上海国际护理大会

作者： Leslie neal-Boylan Yale School of Nursing

＜正＞purpose:Very little is known regarding registered nurses（RN）with sensory *** the *** of Labor Statistics（***）12.9%of individuals between the ages of 21 and 64 years and 30%of individuals between 64 and 75years old have a *** are found in both of these age groups,so while the number of nurses with disabilities is unknown it is likely that the number is significant

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美国现代化客车修理基地

国外铁道车辆 1991年第4期28卷 48-51页

作者： neal Necastro 张雄

本文介绍美国长岛新建的一个现代化客车综合修理基地。该基地的自动化程度较高,采用机器人清洗,可检修转向架、制动机、空调机等各种客车部件。

关键词：维修客车美国车辆

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plant Biotechnology and Genetics : principles, Techniques and Applications

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