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温度对短额负蝗成虫存活率及抗氧化酶活性的影响

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环境昆虫学报 2024年

作者：李文博 nawaz haider bashir 李新畅田睿林史树森张星陈欢欢曲靖师范学院/生物资源与食品工程学院吉林农业大学植物保护学院承德市农林科学院内蒙古师范大学生命科学与技术学院内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室

为明确不同温度对短额负蝗Atractomorpha sinensis成虫存活率以及体内抗氧化酶活性的影响。在24～40℃温度范围内，将短额负蝗健康成虫分组，分别置于生化培养箱处理4 h。测定各温度处理下成虫存活率和抗氧化酶（SOD、CAT、POD）的活性... 详细信息

为明确不同温度对短额负蝗Atractomorpha sinensis成虫存活率以及体内抗氧化酶活性的影响。在24～40℃温度范围内，将短额负蝗健康成虫分组，分别置于生化培养箱处理4 h。测定各温度处理下成虫存活率和抗氧化酶（SOD、CAT、POD）的活性、总抗氧化能力（T-AOC）以及丙二醛（MDA）含量的变化。成虫存活率在24～34℃范围内均高于50%，其他温度处理成虫存活率明显降低。试验温度范围内短额负蝗体内3种抗氧化酶活性随温度升高呈先增后减趋势。32℃时雌、雄成虫的SOD、POD和CAT酶活值最高。T-AOC活性与MDA含量与抗氧化酶活性变化趋势相似，二者均在34℃时达最高。短额负蝗对不同温度的适应性存在差异，温度过高不利于其种群存活，此现象与其体内抗氧化酶活性密切相关。短额负蝗雌成虫抗氧化酶活性高于雄成虫，更能适应温度的变化。

关键词：短额负蝗温度抗氧化酶活性总抗氧化能力丙二醛环境适应性

Crop diversity and pest management in sustainable agriculture

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Journal of Integrative Agriculture 2019年第9期18卷 1945-1952页

作者： HE Han-ming LIU Li-na Shahzad Munir nawaz haider bashir WANG Yi YANG Jing LI Cheng-yun State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan/Faculty of Plant Protection Yunnan Agricultural University

Large-scale crop monocultures facilitate the proliferation and increasing prevalence of diseases and pest *** studies highlight the impacts of plant diversification upon pathogens,and the population dynamics of insects and beneficial organism in agricultural *** studies provide evidence that habitat manipulation techniques such as intercropping,relay,and rotation can significantly improve disease and pest *** review introduces the concept of crop diversity,considers recent insights and mechanisms underlying crop diversity,and discusses its potential for improving sustainable agricultural ***,the phytobiomes resulting from increased crop diversity are increasingly recognized for their contribution to disease and pest ***,understanding the interactions between pathogens or pests with their host phytobiome may lead to novel options for the prevention of *** advances in the agricultural systems include:(i)a better understanding of the mechanisms of interactions between crop species and genotypes;(ii)ecological progress including a better understanding of the context-dependency of those interactions;and(iii)the role of microtopographic variation in agricultural systems for priming basal resistance to multiple pests and pathogens by intercropped *** also highlight recent progress in China and the potential options for habitat management and design that enhance the ecological role of biodiversity in agroecosystems.

关键词： crop diversity pattern pest and disease management priming resistance phytobiome holobiont sustainability

Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

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Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain Shahzad Munir Abdullah Jalal Taj Ali Khan Niaz Muhammad Bahar Ullah Khattak Abdullah Khan Irfan Ahmed Zulqarnain Baloch nawaz haider bashir Muhammad Ameen Jamal Kashif Rahim Humaira Mazhar Maira Riaz Noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

云南不同生态区玉米害虫优势种及其天敌优势种的种群动态

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中国植保导刊 2018年第9期38卷 31-37页

作者：禹田贾永超柴正群吴迪 nawaz haider bashir 马丽陈国华李强云南农业大学植物保护学院云南昆明650201 青岛农业大学经济与管理学院山东青岛266109 云南农业大学热带作物学院云南普洱665000

通过对滇东北温凉地区和滇南暖热地区夏玉米田害虫及其天敌种群的系统调查,明确了云南南北两地玉米上的害虫优势种及其天敌优势种的发生动态规律,以期为玉米害虫的预测预报及防治提供理论依据。结果表明,滇东北昭通地区害虫优势种是玉米... 详细信息

通过对滇东北温凉地区和滇南暖热地区夏玉米田害虫及其天敌种群的系统调查,明确了云南南北两地玉米上的害虫优势种及其天敌优势种的发生动态规律,以期为玉米害虫的预测预报及防治提供理论依据。结果表明,滇东北昭通地区害虫优势种是玉米蚜,发生高峰期为抽雄散粉期和蜡熟期;天敌优势种是异色瓢虫,发生高峰期滞后于玉米蚜。滇南普洱地区玉米抽雄散粉期之前害虫优势种为黄脸油葫芦,发生高峰期为小喇叭口期;抽雄散粉期之后害虫优势种是玉米蚜,发生高峰期为完熟期;天敌优势种是异色瓢虫和黑带食蚜蝇,两种天敌的发生高峰期和玉米蚜吻合。因此,昭通地区防治重点为玉米蚜,防治适期为玉米抽雄散粉期之前。普洱地区防治重点为黄脸油葫芦,可从从苗期开始采取防治措施;玉米蚜的发生时间较晚、为害较轻,可在玉米吐丝期之后对其进行防治。田间施药的时间应注意避开天敌的发生高峰期。

关键词：玉米害虫优势种天敌优势种种群动态

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玉米品种抗蚜性鉴定评价方法与标准研究

环境昆虫学报 2018年第6期40卷 1396-1403页

作者：禹田贾永超柴正群吴迪 nawaz haider bashir 马丽陈国华李强云南农业大学植物保护学院昆明650201 青岛农业大学经济与管理学院山东青岛266109 云南农业大学热带作物学院云南普洱665000

本研究以玉米蚜为研究对象,在云南省昭通地区通过田间调查和相关数据分析,研究了玉米蚜田间种群动态,评价了27个不同品种玉米的抗蚜性,探索了更为简便的玉米抗蚜性评价方法。结果表明:玉米蚜在田间的发生有两个高峰期,分别是抽雄散粉期... 详细信息

本研究以玉米蚜为研究对象,在云南省昭通地区通过田间调查和相关数据分析,研究了玉米蚜田间种群动态,评价了27个不同品种玉米的抗蚜性,探索了更为简便的玉米抗蚜性评价方法。结果表明:玉米蚜在田间的发生有两个高峰期,分别是抽雄散粉期和蜡熟期;采用玉米整个生育期蚜量比值法比较不同品种玉米的抗性,发现高抗品种有6个、抗性品种有6个、中抗品种有6个、感虫品种有4个、高感品种有5个,玉米蚜的种群数量在不同品种玉米间存在显著差异;采用玉米蚜暴发时期到玉米完熟期的蚜量比值来评价玉米抗蚜性可行。

关键词：玉米品种玉米蚜蚜量比值种群动态抗虫性

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曲靖市蝗虫物种多样性调查

曲靖师范学院学报 2023年第6期42卷 21-25,31页

作者：李文博 nawaz haider bashir 琪勒莫格杜国川柳添杰李小花郭效琼李强曲靖师范学院生物资源与食品工程学院云南曲靖655011 包头师范学院生物科学与技术学院内蒙古包头014030 曲靖师范学院文化旅游学院云南曲靖655011 吉林农业大学植物保护学院吉林长春130118

为了解云南曲靖市蝗总科Acridoidea昆虫资源情况,采用五点随机取样法和传统分类学对本地蝗虫物种多样性及其分布进行调查.共采集标本400头,经鉴定隶属7科16属19种,其中斑翅蝗科Oedipodidae属种数量最多(7属7种),其次是锥头蝗科Pyrgomorp... 详细信息

为了解云南曲靖市蝗总科Acridoidea昆虫资源情况,采用五点随机取样法和传统分类学对本地蝗虫物种多样性及其分布进行调查.共采集标本400头,经鉴定隶属7科16属19种,其中斑翅蝗科Oedipodidae属种数量最多(7属7种),其次是锥头蝗科Pyrgomorphidae(1属3种)和网翅蝗科Arcypteridae(2属3种),蝗科Acrididae和瘤锥蝗科Chrotogonidae均为1属1种.多样性分析结果表明马龙区农场蝗虫丰富度、Shannon-Wiener多样性指数、均匀度和优势度指数最高.从物种分布看,短额负蝗Atractomorpha sinensis和疣蝗Trilophidia annulata分布最广.从分布地点看,马龙区农场物种最丰富(17种).研究结果表明曲靖市蝗种多样性及分布特点与生境植被类型密切相关.结果为本地蝗虫的资源利用、预测预报及综合防治提供依据.

关键词：曲靖蝗虫物种多样性

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纺梭负蝗过冷却能力和低温存活率研究

北方农业学报 2024年第3期52卷 108-113页

作者：李文博 haider bashir nawaz 王自杰唐佳威杨爽陈欢欢田睿林曲靖师范学院生物资源与食品工程学院云南曲靖655011 黑龙江省农业科学院佳木斯分院黑龙江佳木斯154007 谱尼测试集团股份有限公司吉林长春130103 内蒙古师范大学生命科学与技术学院内蒙古呼和浩特010022

【目的】明确纺梭负蝗(Atractomorpha burri)种群的耐寒能力。【方法】利用过冷却点测定仪分别测定云南省曲靖市纺梭负蝗各虫态过冷却能力和低温条件下低龄和老龄若虫的存活率。【结果】不同虫态纺梭负蝗的过冷却点和结冰点差异显著(P<0... 详细信息

【目的】明确纺梭负蝗(Atractomorpha burri)种群的耐寒能力。【方法】利用过冷却点测定仪分别测定云南省曲靖市纺梭负蝗各虫态过冷却能力和低温条件下低龄和老龄若虫的存活率。【结果】不同虫态纺梭负蝗的过冷却点和结冰点差异显著(P<0.05),卵期过冷却点和结冰点最低,分别为-16.16、-8.13℃;3龄若虫的过冷却点和结冰点最高,分别为-8.35、-5.20℃。雄性成虫的过冷却点和结冰点分别为-10.26、-7.18℃,低于雌性成虫的-9.00、-7.00℃。过冷却点和结冰点在同一虫态不同个体中的变异符合正态分布。3龄若虫和5龄若虫在低温条件下达到50%以上死亡率的温度分别为-7.00、-9.00℃。【结论】纺梭负蝗卵期的耐寒性最强,5龄若虫具有更强的低温耐受性,雄性成虫抗寒能力强于雌性成虫。

关键词：纺梭负蝗过冷却能力低温存活率

National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan Ahmed Muhammad Atique Usman Iqbal Aujla Muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt Abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib haider Siraj haider Syed Mujahid Hassan Adnan Abdul Jabbar Aman nawaz Khan Muhammad Shoaib Khan Muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad Karim Malik Kamran Rashid Sohail Rashid Mohammad Salih Abdullah Saeed Amjad Salamat Ghias-un-Nabi Tayyab Aasim Yusuf Haseeb haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

Experimental and computational study of annealed nickel sulfide quantum dots for catalytic and antibacterial activity

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Nano Materials Science 2024年第3期6卷 355-364页

作者： Muhammad Ikram Sawaira Moeen Ali haider Anwar Ul-Hamid Haya Alhummiany Hamoud H.Somaily Souraya Goumri-Said Mohammed Benali Kanoun Solar Cell Applications Research Lab Department of PhysicsGovernment College University LahoreLahore54000PunjabPakistan Department of Clinical Sciences Faculty of Veterinary and Animal SciencesMuhammad Nawaz ShareefUniversity of Agriculture66000MultanPunjabPakistan Core Research Facilities King Fahd University of Petroleum&MineralsDhahran31261Saudi Arabia Department of Physics Faculty of ScienceUniversity of JeddahP.O.Box 13151Jeddah21493Saudi Arabia Department of Physics Faculty of ScienceKing Khalid UniversityP.O.Box 9004AbhaSaudi Arabia Physics Department College of Science and General StudiesAlfaisal UniversityP.O.Box 50927Riyadh11533Saudi Arabia Department of Mathematics and Sciences College of Humanities and SciencesPrince Sultan UniversityP.O.Box 66833Riyadh11586Saudi Arabia

This research investigates the hydrothermal synthesis and annealing duration effects on nickel sulfide(NiS_(2) quantum dots(QDs)for catalytic decolorization of methylene blue(MB)dye and antimicrobial *** size increased with longer annealing,reducing catalytic ***–vis,XRD,TEM,and FTIR analyses probed optical structural,morphological,and vibrational *** confirmed NiS2's anorthic structure,with crystallite size growing from 6.53 to 7.81 nm during extended ***–Vis exhibited a bathochromic shift,reflecting reduced band gap energy(Eg)in NiS_(2).TEM revealed NiS_(2)QD formation,with agglomerated QD average size increasing from 7.13 to 9.65 nm with prolonged *** NiS_(2) showed significant MB decolorization(89.85%)in acidic *** NiS_(2) QDs demonstrated notable antibacterial activity,yielding a 6.15mm inhibition zone against Escherichia coli(***)compared to ***-principles computation supported a robust interaction between MB and NiS_(2),evidenced by obtained adsorption *** study highlights the nuanced relationship between annealing duration,structural changes,and functional properties in NiS_(2)QDs,emphasizing their potential applications in catalysis and antibacterial interventions.

关键词： NiS_(2) antibacterial quantum dots dye degradation DFT