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Histological Features of Parotid Gland of Albino Rats Exposed to. Smokeless Tobacco

Journal of Northeast Agricultural University(English Edition) 2016年第3期23卷 54-60页

作者： Sana Murtaza Khoso Pervez Ahmed Khoso navaid qazi Mir Hassan Khoso Ahmed Sultan Jatoi Ubedullah Kaka Dental College Isra University Hyderabad Sindh Pakistan College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand Biopharmaceutical Lab College of Life Sciences Northeast Agricultural University Harbin 150030 China

Smokeless tobacco is very common personal habit of people living in various areas of Asia which is an alarming sign for the development of different oral diseases in such people. The aim of present study was to investigate effects of smokeless tobacco on parofid glands of the Aalbano rats by using various percentages of the smokeless tobacco. The rats were divided into three different groups, control group （A） no smokeless tobacco, experimental group （B） 5% smokeless tobacco and experimental group （C） 10% of smokeless tobacco with different feeds required according parameters. Weekly weight gain and partid gland were analyzed through student P test and histological structures were recorded through HE stain and Retculin stain. The results showed that as compared to control group body, weight of the rats was decreased in groups B and C having smokeless tobacco percentage in the diet. Weight of parotid gland as compared to control group was decreased in groups B and C with diet of smokeless tobacco. Further, histological observation under HE stain showed that parofid gland of group B showed mild narrowing of ductal lumen, collapse of vessels and stromal was also increased, in group C parenchyrnal tissues with loss of acini found damaged and glandular dystrop and lymphatic infiltration were determined moderate to severe. Meanwhile, reticulin stain showed that vascular collapses were shown because of increasing in stromal glandular atrophy in group C as compared to control group. In conclusion, this study showed that smokeless tobacco caused serious injuries in the tissue level in parotid gland with high percentage of smokeless tobacco which highlight health hazards on its consumption.

关键词： smokeless tobacco histological observation parotid gland Albino rat

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