检索结果-南通市图书馆

Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

Microsystems & Nanoengineering 2018年第1期4卷 498-508页

作者： Masato Saito natsuko uchida Shunsuke Furutani Mizuho Murahashi Wilfred Espulgar Naoki Nagatani Hidenori Nagai Yuki Inoue Tomohiko Ikeuchi Satoshi Kondo Hirotaka Uzawa Yasuo Seto Eiichi Tamiya Department of Applied Physics Graduate School of EngineeringOsaka University2-1YamadaokaSuitaOsaka 565-0871Japan Advanced Photonics and Biosensing Open Innovation Laboratory(PhotoBIO-OIL) National Institute of Advanced Industrial Science and Technology(AIST)Photonics Center Osaka University P3 Bldg2-1 Yamada-OkaSuitaOsaka 565-0871Japan Nanomaterials Research Institute National Institute of Advanced Industrial Science and Technology(AIST)1-1-1 HigashiTsukuba 305-8565Japan Health Research Institute National Institute of Advanced Industrial Science and Technology(AIST)1-8-31 MidorigaokaIkedaOsaka 563-8577Japan Department of Applied Chemistry Graduate School of EngineeringOkayama University of Science1-1Ridai-choKita-kuOkayama-shi 700-0005Japan National Research Institute of Police Science 6-3-1 KashiwanohaKashiwaChiba 277-0882Japan

The threat of biological and chemical terror acts remains a growing worldwide *** is therefore a need to develop appropriate technology for the detection of chemical and biological warfare agents,with early identification intended for use by first ***,we disclose the developed autonomous air sampling and detection system for evaluation of the presence of chemical and biological warfare agents that can be harmful to the *** current device utilizes the designed mist generator-assisted air collection system(338 l min^(−1))and biosensing chip technologies,such as electrochemical measurement,Au nanoparticle-based localized surface plasmon resonance,and rapid microfluidic chip PCR for detection of minute concentrations lower than the mean lethal dose(LD_(50))of nerve gases(sarin and VX),toxic proteins(BTX/A/Hc and ricin),and pathogens(anthrax simulant).An operation time of only 5–15 min is needed for the collection and detection;sample preparation is already integrated into the system without the need for direct human *** addition to the system’s sensitivity and ease of use,its portability makes it highly beneficial for first responders,which could aid in immediate risk assessment and mitigation of on-site events.

关键词： biological and chemical agents biosensing electrochemical detection LSPR PCR rapid detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Elastography for the pancreas: Current status and future perspective

引用

World Journal of Gastroenterology 2016年第14期22卷 3712-3724页

作者： natsuko Kawada Sachiko Tanaka Department of Gastroenterology Nissay Hospital Osaka 550-0012 Japan Osaka Center for Cancer and Cardiovascular Diseases Prevention Osaka 550-0012 Japan

Elastography for the pancreas can be performed by either ultrasound or endoscopic ultrasound(EUS). There are two types of pancreatic elastographies based on different principles, which are strain elastography and shear wave elastography. The stiffness of tissue is estimated by measuring the grade of strain generated by external pressure in the former, whereas it is estimated by measuring propagation speed of shear wave, the transverse wave, generated by acoustic radiation impulse(ARFI) in the latter. Strain elastography is difficult to perform when the probe, the pancreas and the aorta are not located in line. Accordingly, a fine elastogram can be easily obtained in the pancreatic body but not in the pancreatic head and tail. In contrast, shear wave elastography can be easily performed in the entire pancreas because ARFI can be emitted to wherever desired. However, shear wave elastography cannot be performed by EUS to date. Recently, clinical guidelines for elastography specialized in the pancreas were published from Japanese Society of Medical Ultrasonics. The guidelines show us technical knacks of performing elastography for the pancreas.

关键词： Elastography Pancreas Strain Shear wave

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enzyme-instructed self-assembly of peptides containing phosphoserine to form supramolecular hydrogels as potential soft biomaterials

引用

Frontiers of Chemical Science and Engineering 2017年第4期11卷 509-515页

作者： Jie Zhou Xuewen Du Jiaqing Wang natsuko Yamagata Bing Xu Department of Chemistry Brandeis University 415 South St. Waltham MA 02453 USA.

Enzyme-instructed self-assembly （EISA） offers a facile approach to explore the supramolecular assemblies of small molecules in cellular milieu for a variety of biomedical applications. One of the commonly used enzymes is phosphatase, but the study of the substrates of phosphatases mainly focuses on the phos- photyrosine containing peptides. In this work, we examine the EISA of phosphoserine containing small peptides for the first time by designing and synthesizing a series of precursors containing only phosphoserine or both phos- phoserine and phosphotyrosine. Conjugating a phospho- serine to the C-terminal of a well-established self- assembling peptide backbone, （naphthalene-2-1y）-acetyl- diphenylalanine （NapFF）, affords a novel hydrogelation precursor for EISA. The incorporation ofphosphotyrosine, another substrate of phosphatase, into the resulting precursor, provides one more enzymatic trigger on a single molecule, and meanwhile increases the precursors＇ propensity to aggregate after being fully dephosphorylated. Exchanging the positions of phosphorylated serine and tyrosine in the peptide backbone provides insights on how the specific molecular structures influence self-assembling behaviors of small peptides and the subsequent cellular responses. Moreover, the utilization of D-amino acids largely enhances the biostability of the peptides, thus providing a unique soft material for potential biomedical applications.

关键词： enzyme-instructed self-assembly phosphoser-ine phosphatase supramolecular hydrogel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Particle size distribution of brominated flame retardants in house dust from Japan

引用

Emerging Contaminants 2016年第2期2卷 109-117页

作者： natsuko Kajiwara Hidetaka Takigami Center for Material Cycles and Waste Management Research National Institute for Environmental Studies16-2 OnogawaTsukubaIbaraki 305-8506Japan

The present study was conducted to examine the concentrations,profiles,and mass distributions of polybrominated diphenyl ethers(PBDEs),hexabromocyclododecanes(HBCDs),and polybrominated dibenzo-p-dioxins/furans(PBDD/Fs)based on the particle sizes of house dust samples from five homes in *** removal of impurities from house dust from vacuum cleaner bags,selected indoor dust samples were size fractionated(>2 mm,1-2 mm,0.5-1 mm,250-500 mm,106-250 mm,53-106 mm,and250 mm in size and fluffy dust were *** conclusion is that particulate dust<250 mm in size without fluffy dust should be used to analyze dust for brominated flame retardants.

关键词： Brominated flame retardant PBDEs HBCDs House dust Particle size

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Porphyromonas gingivalis,a periodontal pathogen,impairs post-infarcted myocardium by inhibiting autophagosome–lysosome fusion

引用

International Journal of Oral Science 2023年第4期15卷 626-639页

作者： Yuka Shiheido-Watanabe Yasuhiro Maejima Shun Nakagama Qintao Fan natsuko Tamura Tetsuo Sasano Department of Cardiovascular Medicine Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityTokyoJapan

While several previous studies have indicated the link between periodontal disease (PD) and myocardial infarction (MI), theunderlying mechanisms remain unclear. Autophagy, a cellular quality control process that is activated in several diseases, includingheart failure, can be suppressed by Porphyromonas gingivalis (P.g.). However, it is uncertain whether autophagy impairment byperiodontal pathogens stimulates the development of cardiac dysfunction after MI. Thus, this study aimed to investigate therelationship between PD and the development of MI while focusing on the role of autophagy. Neonatal rat cardiomyocytes(NRCMs) and MI model mice were inoculated with wild-type P.g. or gingipain-deficient P.g. to assess the effect of autophagyinhibition by P.g. Wild-type P.g.-inoculated NRCMs had lower cell viability than those inoculated with gingipain-deficient P.g. Thisstudy also revealed that gingipains can cleave vesicle-associated membrane protein 8 (VAMP8), a protein involved in lysosomalsensitive factor attachment protein receptors (SNAREs), at the 47th lysine residue, thereby inhibiting autophagy. Wild-type P.g.-inoculated MI model mice were more susceptible to cardiac rupture, with lower survival rates and autophagy activity thangingipain-deficient P.g.-inoculated MI model mice. After inoculating genetically modified MI model mice (VAMP8-K47A) with wildtype P.g., they exhibited significantly increased autophagy activation compared with the MI model mice inoculated with wild-typeP.g., which suppressed cardiac rupture and enhanced overall survival rates. These findings suggest that gingipains, which arevirulence factors of P.g., impair the infarcted myocardium by cleaving VAMP8 and disrupting autophagy. This study confirms thestrong association between PD and MI and provides new insights into the potential role of autophagy in this relationship.

关键词： inhibiting myocardium periodontal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nicotine Treatment Induces Expression of Matrix Metalloproteinases in Human Osteoblastic Saos-2 Cells

引用

Acta Biochimica et Biophysica Sinica 2006年第12期38卷 874-882页

作者： Tomoko KATONO Takayuki KAWATO natsuko TANABE Naoto SUZUKI Kazuhiro YAMANAKA Hitoshi OKA Masafumi MOTOHASHI Masao MAENO Kanda Surugadai Chiyoda-kuTokyo 101-8310Japan Department of Oral Health Sciences Nihon UniversitySchool of DentistryTokyo 101-8310Japan Japan Department of Biochemistry Nihon University School of Dentistry.Tokyo 101-8310Japan

Tobacco smoking is an important risk factor for the development of severe ***,we showed that nicotine affected mineralized nodule formation,and that nicotine andlipopolysaccharide stimulated the formation of osteoclast-like cells by increasing production of macrophagecolony-stimulating factor （M-CSF） and prostaglandin E2（PGE2） by human osteoblastic Saos-2 *** thepresent study,we examined the effects of nicotine on the expression of matrix metalloproteinases （MMPs）,tissue inhibitors of matrix metalloproteinases （TIMPs）,the plasminogen activation system including thecomponent of tissue-type plasminogen activator （tPA）,urokinase-type PA （uPA）,and PA inhibitor type 1（PAI-1）,α7 nicotine receptor,and *** also examined the effect of the nicotine antagonist D-tubocurarineon nicotine-induced expression of *** expression was examined using real-time polymerase chainreaction （PCR） to estimate mRNA *** addition,expression of the MMP,TIMP,uPA,tPA,and PAI-1proteins was determined by Western blotting *** treatment caused expression of MMP-1,2,3,and 13,but not MMP-14,to increase significantly after 5 or 10 d of culture;MMP-14 expression did notchange through day *** of MMP-1 expression by nicotine treatment was eliminated bysimultaneous treatment with *** the presence of nicotine,expression of uPA,PAI-1,orTIMP-1,2,3,or 4 did not change over 14 d of culture,whereas expression of tPA increased significantly byday *** also increased expression of the α7 nicotine receptor and c-fos *** results suggestthat nicotine stimulates bone matrix turnover by increasing production of tPA and MMP-1,2,3,and 13,thereby tipping the balance between bone matrix formation and resorption toward the latter process.

关键词： nicotine matrix metalloproteinase tissue inhibitor of matrix metalloproteinase plasminogen activation system

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Lipopolysaccharide Enhances the Production of Nicotine-Induced Prostaglandin E2 by an Increase in Cyclooxygenase-2 Expression in Osteoblasts

引用

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 163-172页

作者： Maiko SHOJI natsuko TANABE Narihiro MITSUI Naoto SUZUKI Osamu TAKEICHI Tomoko KATONO Akira MOROZUMI Masao MAENO Department of Oral Health Sciences Nihon University School of DentistryTokyo 101-8310Japan Division of Functional Morphology Dental Research Center.Nihon University School ofDentistryTokyo 101-8310Japan Nihon University Grndudte School of Dentistry Tokyo 101-8310Japan Division of Functional Morphology Dental Research Center.Nihon University School of DentistryTokyo 101-8310Japan Department of Endodontics Nihon University School of DentisityTokyo 101-8310Japan Division of Advanced Dental Treatment Dental Research CenterNihon University School of DentistryTokyo 101-8310Japan Nihon University Graduate School of Dentistry Tokyo 101-8310Japan

Previous studies have indicated that lipopolysaccharide（LPS）from Gram-negative bacteria inplaque induces the release of prostaglandin E2（PGE2）,which promotes alveolar bone resorption in periodontitis,and that tobacco smoking might be an important risk factor for the development and severity of *** determined the effect of nicotine and LPS on alkaline phosphatase（ALPase）activity,PGE2production,and the expression of cyclooxygenase（COX-1,COX-2）,PGE2receptors Ep1-4,and macrophage colonystimulating factor（M-CSF）in human osteoblastic Saos-2 *** cells were cultured with 10-3M nicotinein the presence of 0,1,or 10μg/ml LPS,or with LPS *** activity decreased in cells cultured withnicotine or LPS alone,and decreased further in those cultured with both nicotine and LPS,whereas PGE2production significantly increased in the former and increased further in the *** itself,nicotine did notaffect expression of COX-1,COX-2,any of the PGE2receptors,or M-CSF,but when both nicotine and LPSwere present,expression of COX-2,Ep3,Ep4,and M-CSF increased *** addition of10-4M indomethacin eliminated the effects of nicotine and LPS on ALPase activity,PGE2production,and M-CSF *** of protein kinase A was high in cells cultured with nicotine and *** suggest that LPS enhances the production of nicotine-induced PGE2by an increase in COX-2 expres-sion in osteoblasts,that nicotine-LPS-induced PGE2interacts with the osteoblast Ep4 receptor primarily inautocrine or paracrine mode,and that the nicotine-LPS-induced PGE2then decreases ALPase activity andincreases M-CSF expression.

关键词： nicotine lipopolysaccharide prostaglandin E2 cyclooxygenase-2 Ep4 receptor

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Study of the Conditions Surrounding Fatigue That Are Common to Medical Professionals Working in Emergency and Critical Care Centers in Japan

引用

Health 2020年第1期12卷 27-37页

作者： natsuko Makino Department of Nursing School of Health Sciences Sapporo Medical University Sapporo Japan

This study aimed to clarify the conditions surrounding fatigue that are common to medical professionals working in emergency and critical care centers in Japan. Semi-structured interviews were conducted with eight professionals ranging from doctors, nurses and pharmacists to clinical engineering technologists and radiation technologists. Their narratives were analyzed using the qualitative descriptive approach to determine fatigue common to all professionals and the reasons behind it. The five categories that emerged as the reasons for fatigue common to the subjects were [playing one’s role in treatment and procedures for emergency and critical patients], [accommodating the patient’s background and coming to terms with the outcome], [difficulties in liaising with other professionals], [feeling pressure as a responsible professional in emergency care] and [loss of sense of time caused by variable working hours]. The results revealed that fatigue common to all of the subjects was related to dealing with patients, coordinating with other professionals, having professional responsibilities and the working environment. This study suggests that arrangements to improve the working environment, ensure adequate staffing, and provide mental health support for the well-being of medical professionals working in emergency and critical care centers are necessary.

关键词： Fatigue Medical Professionals Emergency and Critical Care Centers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

引用

Open Journal of Nursing 2020年第11期10卷 1038-1047页

作者： natsuko Kojima Kyoko Asazawa Division of Nursing Faculty of Nursing Tokyo Healthcare University Tokyo Japan

Background: The aim of this study was to investigate Japanese students and employed adults’ characteristic features, and sexual situation, and to find the correlated factors of lesbian, gay, bisexual, transgender (LGBT) recognition in order to obtain suggestions for improved relationships with sexual minorities in a diversifying society. Methods: This quantitative cross-sectional descriptive correlational study occurred during 2018 to 2019 when 2210 questionnaires were distributed to a purposive sample of eligible participants. A total of 1534 completed questionnaires (69.4%) were returned, of which 1439 (65.1%) were suitable for analyses. The survey included respondents’ 1) characteristics, 2) sexual situation, and 3) LGBT recognition scale. Data were analyzed with descriptive statistics, factor analysis, reliability analysis, and structural equation modeling. SPSS ver. 26.0 (SPSS, Chicago, IL, USA) was used for data analysis at a 5% significance level. Results: The LGBT recognition score was significantly affected by suffering from gender identity (standardized partial regression coefficient: β = 0.17, p −0.19, p Conclusions: Suffering from gender identity, learning about LGBT experience, experience of disclosure from LGBT person, sexual problem with intimate relationship, and diversity penetration had a positive effect, whereas age had negative effects on the LGBT recognition score.

关键词： Sexual and Gender Minorities Cross-Sectional Studies Covariance Structure Analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Predictors of Mothers’ Early Postpartum Fatigue: A Cross-Sectional Study

引用

Open Journal of Nursing 2020年第9期10卷 890-902页

作者： natsuko Kojima Kyoko Asazawa Division of Nursing Faculty of Nursing Tokyo Healthcare University Tokyo Japan

Background: Most postpartum mothers experience fatigue. However, it was unclear which day after birth during hospitalization mothers are the most fatigued, and if the mothers’ background is a factor. The aim of this study was to investigate hospitalized mothers’ characteristic features, fatigue, and relaxation, and then to find the correlated factors of fatigue in order to obtain suggestions for mothers’ early postpartum care. Methods: This quantitative cross-sectional descriptive correlational study occurred during May to December 2016. A purposive sample of early postpartum hospitalized mothers at a postpartum unit in a Tokyo hospital completed an anonymous self-filled questionnaire survey. The survey included respondents’ characteristics, fatigue scale, and relaxation scale. Data were analyzed with descriptive statistics, independent t-test, one-way analysis of variance, multiple regression analyses, and structural equation modeling. Results: Returned questionnaires were 251 (83.4%). Fatigue was significantly higher for mothers with longer postpartum days compared to shorter postpartum days (F = 5.7, p β = −0.30, p β = 0.26, p β = 0.14, p Conclusions: No relaxation, longer postpartum days, and previous disease were associated with Fatigue for early postpartum mothers. These results suggest that focusing on previous disease, length of postpartum days, and mother’s relaxation is needed to provide full support to early postpartum mothers.

关键词： Postpartum Mothers Fatigue Relaxation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：