检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

广西地区野鼠戊型肝炎病毒感染情况调查

微生物学免疫学进展 2008年第4期36卷 40-42页

作者：樊金萍李艳萍宋爱京李荣成杨进业王佑春中国药品生物制品检定所北京100050 广西壮族自治区疾病预防控制中心南宁530021

从广西壮族自治区5个市采集256份野鼠肝脏和对应的血清211份,采用HEV抗体(HEV-Ab)酶联免疫试剂盒(双抗原夹心法)对血清样本进行抗-HEV检测,并对不同地区HEV抗体阳性率进行χ2检验。采用HEV核酸荧光PCR试剂盒对肝脏样本进行HEV核酸检测... 详细信息

从广西壮族自治区5个市采集256份野鼠肝脏和对应的血清211份,采用HEV抗体(HEV-Ab)酶联免疫试剂盒(双抗原夹心法)对血清样本进行抗-HEV检测,并对不同地区HEV抗体阳性率进行χ2检验。采用HEV核酸荧光PCR试剂盒对肝脏样本进行HEV核酸检测。结果显示,211份野鼠血清抗-HEV的抗体阳性率为6.6%,其中百色市的抗体阳性率最高,为14.1%,其他地区抗体阳性率均在10%以下。256份野鼠肝脏中未检测到HEV核酸。由此可见,广西壮族自治区5个市的野鼠中存在HEV感染,但其是否为HEV的自然宿主及在戊型肝炎传播中起何作用需进一步研究。

关键词：戊型肝炎病毒抗体阳性率野鼠

prevention and control Measures of Bovine viral Diarrhea Virus in Developed Country and Its Inspiration to china

在线全文

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2010年第8期2卷 21-24页

作者： WANG Wei1,2,LU Wei1,2,WU Hua1,2 1.institute of Special Economic Animal and Plant Science,chinese Academy of Agricultural Sciences,Jilin 132109,china 2.beijing Bigvet Biotech Co.Ltd.,beijing 100176,china

Bovine viral diarrhea disease is an important disease in the world, which causes great economic loss and leads to serious clinical symptoms such as drop in milk and meat production, reproductive failure, growth retardation, and secondary infection increasing the mortality rate. The developed countries have made a series of measures in preventing the disease and made a great purpose. This paper summarizes those methods in order to make a reference for the control of the disease in china.

关键词： Bovine viral diarrhea disease Prevent and control measures Inspiration

Overexpression of α-synuclein caused mitochondria vacuolization and mitochondrial membrene potential to decrease by interacting with voltage-dependent anion channel

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Overexpression of α-synuclein caused mitochondria vacuoliza...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Lingling LU,Yuangang ZHU,Chunyan ZHANG,Xiaohong SUN,Shasha ZHAO,Pusheng GONG,Hui YANG~* beijing institute for Neuroscience,Capital Medical University(CMU),beijing center of Neural Regeneration and Repairing,Key Laboratory for Neurodegenerative diseases of Ministry of Education,beijing 100069,china

＜正＞We previously found that alpha-Synuclein（Syn） localized not only in neuclei but also in axons and cytoplasm,especially enriched in *** of accumulating evidence showing that overexpression of Syn could partly mimic the symptom of Parkison’s disease（PD）,the exact mechanism remains *** find out the subcelluar localization of Syn in substantia nigra（SN） neurons especially on mitochondria and its effect on mitochondria in Syn induced PD rats model may help explain the pathogenesis of Syn in *** the present study,the synuclein gene was introduced into the SN of rats using recombinant adeno-associtaed viral（rAAV） *** immunogold electron microscopic technique,we found that the density of Syn-positive gold particles was greater in both substantia nigra and striatum in PD rat model than in the control *** compare the distribution of Syn in different cell compartment with the control group,the density of Syn-positive gold particles was greater in neuclei, axons,cytoplasms,especially mitochondria in SN of PD rat model,while the density of gold particles decreased to zero in pre-synaptic *** with the SN regeon,the density of gold particles increased mainly in pre-synaptic terminals,cytoplasm and neucleus in PD rat model,with no difference in axons and mitochondria. The changed distribution of Syn in different cell compartments in PD rat model suggested that high content Syn may be involved in axon transport,plasticity of synapse and gene ***,we also observed that mitochondria were damaged when its membrene potential *** an interaction between Syn and voltagedependent anion channel（VDAC） was also *** together,the current data suggested that Syn may be involved in the maintainance of mitochondria membrene potential and energy metabolism by interacting with permeability transition pore（PTP） protein VDAC,which may play a role in PD pathogenesis.

关键词： Parkinson’s disease a-Synuclein electronmicroscope subcellular localizations mitochondria dysfunction

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

灭活SARS病毒的免疫原性的实验研究

现代免疫学 2004年第5期24卷 386-390页

作者：熊盛王一飞张庶民陆家海孟民杰张美英刘新建张传海刘石生李久香万卓越郑焕英鄢心革暨南大学生物医药研究开发基地广州510630 中国药品生物制品检定所北京100050 中山大学广州510275 第一军医大学广州510515 广东省疾病预防控制中心广州510300

研究灭活SARS病毒的免疫原性。SARS病毒F6 9株经甲醛灭活后 ,加入氢氧化铝佐剂 ,以 3种剂量接种BALB/c小鼠 ,定时采血 ,测定特异性抗体的滴度及其中和活性 ,同时用化学发光酶联免疫法测定抗血清与SARS病毒结构蛋白的反应特异性及其相对... 详细信息

研究灭活SARS病毒的免疫原性。SARS病毒F6 9株经甲醛灭活后 ,加入氢氧化铝佐剂 ,以 3种剂量接种BALB/c小鼠 ,定时采血 ,测定特异性抗体的滴度及其中和活性 ,同时用化学发光酶联免疫法测定抗血清与SARS病毒结构蛋白的反应特异性及其相对强度。结果发现 ,小鼠接种疫苗 4d后 ,血清中可检测到IgM抗体 ,直至 2 6d后逐渐下降 ;IgG抗体在初次免疫 8d后出现 ,4 7d时达最高峰 ,6 3d后进入稳定期 ;不同剂量组的抗体滴度具有明显剂效关系 ,低剂量组和中剂量组滴度峰值为 1∶192 0 0 ,高剂量组滴度峰值为 1∶384 0 0。中和实验结果表明 ,小鼠所产生的抗体具有中和病毒活性 ,在 6 3d时 ,低剂量组和中剂量组血清的中和效价为 1∶12 80 ,高剂量组血清的中和效价为 1∶5 12 0。抗体分类结果表明 ,小鼠抗血清中含有针对多种SARS病毒结构蛋白的特异性抗体 ,其中 ,针对N蛋白、S4蛋白和S2蛋白的抗体水平相对较高 ,而抗M抗体、抗 3CL抗体的水平相对较低。上述结果说明 ,SARS病毒F6 9株经甲醛灭活后 ,各主要结构蛋白仍保持较强免疫原性 ;免疫小鼠后 ,可以诱导产生高滴度的特异性混合抗体。

关键词： SARS病毒灭活 IgG 中和

Mutation in the length of Hemagglutinin-Neuraminidase protein is a prerequisite for evolution to virulence of Newcastle disease virus

在线全文

学校读者我要写书评

暂无评论

Mutation in the length of Hemagglutinin-Neuraminidase protei...

1995～2002年阴沟肠杆菌临床分离株的耐药性分析

中国畜牧兽医学会兽医公共卫生学分会成立大会暨第一次学术研讨会

作者： Chen Hongjun~(1#) Ai Jing~($1,2) Gao Qinxue~($2) Ding Chan~(1*) Yu Shengqing~3 Hu Qinghai~1 Song Cuiping~1 Ma Zhiyong~1 1 veterinary public health research center,Shanghai Veterinary Research institute,chinese Academy of Agricultural Sciences,Shanghai,P.R.china 200232 2 jiangsu Animal Husbandry and Veterinary Medicine College,Taizhou,P.R.china 225300 3 Nationai institutes of Health,Bethesda,Maryland,U.S.A 20892 4 Nantong animal diseases preventing and control center,Nantong Veterinary station,25 yaogang road,Nantong,Jiangsu,P.R.china 260006

In previous study,a benign Newcastle disease virus(NDV)isolate from a wild bird was passaged via air sac and brain in chickens,and found that the avirulent virus has the potential to become highly pathogenic NDV,changing with the cleavage site of the fusion protein,leading to the enhancement of *** the study,we aimed to analyze on the mutation of HN proteins produced by the passaged viruses,to investigate whether HN sequence would also change during the avirulent virus passaged in ***,by sequencing the HN complete mRNA fragments,we found the length of HN became deduce by two times passaging via air sac(2a),however,the mutation of F protein cleavage site began from 9a isolate. By hemadsorption and neuraminidase assay,we found that neuraminidase activity increased by passages,however,no remarkable influence on hemadsorption activity in presence of trypsin(2.5μg/mL).It might be related with the point mutations in the HN protein,e.g.Y-H and *** genomic sequencing of the 15 passages will be completely open for understanding other viral proteins in the pathogenicity in the future.

关键词： Newcastle disease virus Hemagglutinin-Neuraminidase protein evolution virulence

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

四川生理科学杂志 2004年第1期26卷 1-4页

作者：马越李景云张新妹许明哲张力胡昌勤金少鸿中国药品生物制品检定所国家细菌耐药性监测中心北京100050

目的 :调查 1 995～ 2 0 0 2年阴沟肠杆菌临床分离株对临床常用抗菌药物的耐药性变化。方法 :药物敏感性试验采用纸片扩散法 ,耐药性数据分析采用WHONET5软件。结果 :1 995年 1月 1日至 2 0 0 2年 1 2月3 1日 ,国家细菌耐药性监测网医... 详细信息

目的 :调查 1 995～ 2 0 0 2年阴沟肠杆菌临床分离株对临床常用抗菌药物的耐药性变化。方法 :药物敏感性试验采用纸片扩散法 ,耐药性数据分析采用WHONET5软件。结果 :1 995年 1月 1日至 2 0 0 2年 1 2月3 1日 ,国家细菌耐药性监测网医院共收集阴沟肠杆菌临床分离菌 5 5 5 8株。标本来源主要为痰、尿液及和伤口及其分泌物 ,分别占 5 3 %～ 65 %、9%～ 1 2 %和 2 %～ 1 3 %。阴沟肠杆菌对临床常用抗菌药物的耐药率 ,除亚胺培南和美洛培南外 ,8年间都有不同程度的增加。头孢曲松、头孢噻肟、头孢他啶、环丙沙星、庆大霉素和阿米卡星等抗菌药物的耐药率增加了 2 0 %以上。 2 0 0 2年的结果表明 ,碳青霉烯类抗生素、阿米卡星、头孢吡肟和头孢哌酮 /舒巴坦对阴沟肠杆菌临床分离株有较好的抗菌活性。此外 ,阴沟肠杆菌临床分离株对头孢噻肟和头孢他啶双重敏感率在逐年下降 ,双重耐药率在逐年增加。头孢噻肟和头孢他啶抑菌圈直径的均值也在逐年减小。结论细菌耐药性问题是抗感染治疗的主要威胁。

关键词： 1995~2002年阴沟肠杆菌临床分离株耐药性分析 WHONET5软件 2002年12月碳青霉烯类抗生素头孢哌酮/舒巴坦药物敏感性试验抗菌药物头孢噻肟头孢他啶临床常用阿米卡星纸片扩散法 1995年临床分离菌细菌耐药性

Comparing different machine learning techniques for predicting COVID-19 severity

在线全文

学校读者我要写书评

暂无评论

Infectious diseases of Poverty 2022年第1期11卷 97-97页

作者： Yibai Xiong Yan Ma Lianguo Ruan Dan Li Cheng Lu Luqi Huang the national Traditional chinese Medicine Medical Team institute of Basic Research in Clinical Medicine China Academy of Chinese Medical SciencesNo.16Nanxiao StreetDongzhimenDongcheng DistrictBeijing100700BeijingChina 不详 national Resource center for chinese Materia Medica China Academy of Chinese Medical SciencesNo.16Nanxiao StreetDongzhimenDongcheng DistrictBeijing100700BeijingChina

Background Coronavirus disease 2019(COVID-19)is still ongoing spreading globally,machine learning techniques were used in disease diagnosis and to predict treatment outcomes,which showed favorable *** present study aims to predict COVID-19 severity at admission by different machine learning techniques including random forest(RF),support vector machine(SVM),and logistic regression(LR).Feature importance to COVID-19 severity were further *** A retrospective design was adopted in the JinYinTan Hospital from January 26 to March 28,2020,eighty-six demographic,clinical,and laboratory features were selected with LassoCV method,Spearman’s rank correlation,experts’opinions,and literature ***,SVM,and LR were performed to predict severe COVID-19,the performance of the models was compared by the area under curve(AUC).Additionally,feature importance to COVID-19 severity were analyzed by the best performance *** A total of 287 patients were enrolled with 36.6%severe cases and 63.4%non-severe *** median age was 60.0 years(interquartile range:49.0–68.0 years).Three models were established using 23 features including 1 clinical,1 chest computed tomography(CT)and 21 laboratory *** three models,RF yielded better overall performance with the highest AUC of 0.970 than SVM of 0.948 and LR of 0.928,RF also achieved a favorable sensitivity of 96.7%,specificity of 69.5%,and accuracy of 84.5%.SVM had sensitivity of 93.9%,specificity of 79.0%,and accuracy of 88.5%.LR also achieved a favorable sensitivity of 92.3%,specificity of 72.3%,and accuracy of 85.2%.Additionally,chest-CT had highest importance to illness severity,and the following features were neutrophil to lymphocyte ratio,lactate dehydrogenase,and D-dimer,*** Our results indicated that RF could be a useful predictive tool to identify patients with severe COVID-19,which may facilitate effective care and further optimize resources.

关键词： COVID-19 Severity Machine learning Support vector machine Random Forest Logistic regression

中国人类免疫缺陷病毒感染者围手术期抗病毒治疗专家共识(第二版)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华实验和临床感染病杂志（电子版） 2021年第5期15卷 289-294页

作者：中国性病艾滋病防治协会学术委员会外科学组中华医学会热带病与寄生虫学分会外科学组无张强不详国家传染病医学中心(北京)

获得性免疫缺陷综合征(AIDS)已经成为我国重大的公共卫生问题之一。人类免疫缺陷病毒(HIV)感染者进行手术治疗时,一方面医务人员职业暴露感染HIV的风险高,另一方面患者免疫功能受损,机会性感染及术后并发症风险增加,手术治疗具有特殊性... 详细信息

获得性免疫缺陷综合征(AIDS)已经成为我国重大的公共卫生问题之一。人类免疫缺陷病毒(HIV)感染者进行手术治疗时,一方面医务人员职业暴露感染HIV的风险高,另一方面患者免疫功能受损,机会性感染及术后并发症风险增加,手术治疗具有特殊性,以上均与HIV感染者病毒载量有关,因此围手术期抗病毒治疗至关重要,为规范HIV感染者围手术期抗病毒治疗,中国性病艾滋病防治协会学术委员会外科学组和中华医学会热带病与寄生虫学分会外科学组于2019年制定了中国人类免疫缺陷病毒感染者围手术期抗病毒治疗专家共识(第一版)(以下简称共识)。本次专家共识是基于近几年最新研究进展,对2019年第一版共识进行修订。主要体现在手术风险评估、围手术期抗病毒方案的选择、机会性感染的预防等方面,尤其强调术前快速降病毒载量和重建患者免疫功能。本专家共识将根据最新的临床研究证据定期进行更新。

关键词：人类免疫缺陷病毒围手术期抗病毒治疗方案病毒载量共识

新生儿遗传代谢病筛查实验室检测技术规范专家共识

在线全文

学校读者我要写书评

暂无评论

中华新生儿科杂志（中英文） 2023年第8期38卷 449-454页

作者：中华预防医学会出生缺陷预防与控制专业委员会新生儿遗传代谢病筛查学组国家卫生健康委员会临床检验中心新生儿遗传代谢病筛查室间质评专业委员会赵正言王治国不详浙江大学医学院附属儿童医院遗传与代谢科国家儿童健康与疾病临床医学研究中心杭州310052 北京医院室间质评室国家老年医学中心/国家卫生健康委临床检验中心中国医学科学院老年医学研究院北京100730

我国遗传代谢病筛查实验室众多,在仪器配置、检测方法、阳性切值、管理流程及质量控制等方面存在差异,不同实验室间的检测结果难以比较。为实现同质化管理,提高筛查质量,中华预防医学会出生缺陷预防与控制专业委员会新生儿遗传代谢病筛... 详细信息

我国遗传代谢病筛查实验室众多,在仪器配置、检测方法、阳性切值、管理流程及质量控制等方面存在差异,不同实验室间的检测结果难以比较。为实现同质化管理,提高筛查质量,中华预防医学会出生缺陷预防与控制专业委员会新生儿遗传代谢病筛查学组和国家卫生健康委员会临床检验中心新生儿遗传代谢病筛查室间质评专业委员会,联合制定新生儿遗传代谢病筛查实验室检测技术规范共识,为全国筛查实验室提供质量管理标准,促进筛查技术的规范化应用。

关键词：新生儿遗传代谢病筛查实验室检测技术规范共识