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The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth

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Science china(Life Sciences) 2004年第1期47卷 25-30页

作者： GU Zhiliang, ZHU Dahai, LI Ming, LI Hui, DENG Xuemei & WU ChangxinMolecular and Cellular Developmental Biology Laboratory, Harbin Institute of Technology, Harbin 150001, china College of Animal Science & Technology, Northeast agricultural university, Harbin 150030, china national laboratories for agribiotechnology, china agricultural university, beijing 100094, china College of Animal Science & Technology, china agricultural university, beijing 100094, china Department of Life Science and Technology, Changshu Institute of Technology, Changshu 215500, china Department of Biochemistry and Molecular Biology, national Laboratory of Medical Molecular Biology, Peking Union Medical College, beijing 100005, china

Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P < 0.05). Breast muscle percentage (BMP) of the AA type is higher than that of the AB type. The breast muscle weight and breast muscle percentages of F2 individuals have significant difference between CC and DD genotypes (P< 0.05). Breast muscle weight (BMW) of EF birds is higher than that of EE birds (P< 0.05). In this report, we present the first genetic evidence to show that chicken myostatin not only plays an important role in controlling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.

关键词： myostatin gene, SNPs, F2 chicken population, abdominal fat, breast muscle weight.

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Tissue-specific expression of glutathione S-transferases induced by 2-tridecanone or quercetin in cotton bollworms, Helicoverpa armigera (Hübner)

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Progress in Natural Science:Materials International 2005年第11期15卷 988-992页

作者： TANG Fang, LIANG Pei and GAO Xiwu (Department of Entomology, china agricultural university, beijing 100094, china) Department of Entomology China Agricultural University Beijing 100094 China

The tissue-specific expression of glutathione S-transferases (GSTs) in the cotton bollworm and the expression level induced by 2-tridecanone and quercetin were examined using the methods of biochemistry and the quantitative PCR. The relative expression level of GST mRNA was unanimous with the GSTs activity conjugaging with 1-chloro-2, 4-dimitro-benzene (CDNB) in fat bodies, midguts, heads and integuments of cotton bollworms. The GSTs activity in fat bodies was the highest, then midguts, heads and integuments in turn, which was in consistent with the relative expression level of GST mRNA. The specific activity of GSTs and the relative expression level of GST mRNA could be significantly induced by 2-tridecanone and quercetin, and after the induction the order of the GSTs activity and the relative expression level of GST mRNA in the above four tissues in cotton bollworms was not different from the control. The induction of GSTs by 2-tridecanone was stronger than by quercetin in all four tissues, which was in accordance with the relative expression level of GST mRNA. It suggested that the increase of GSTs activity induced by plant allelochemicals was associated with the elevated expression of GST mRNA in cotton bollworms.

关键词： Helicoverpa armigera glutathione S-transferase tissue-specific expression plant allelochemicals.

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Genetic analysis on 3'- terminal flanking region of uncoupling protein 3 in different pig breeds

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Chinese Science Bulletin 2002年第18期47卷 1541-1543页

作者： FANG Meiying, ZHAO Xingbo, LI Ning & WU ChangxinCollege of Animal Science and Technology, china agricultural university, beijing 100094, china State Key Laboratory for Agrobiotechnology, china agricultural university, beijing 100094, china College of Animal Science and Technology China Agricultural University Beijing China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing China

The 3’-terminal flanking region of porcine uncoupling protein 3 (UCP3) was cloned, the sequence data revealed 15 nucleotide substitutions among Landrace and three Chinese native pig breeds named Neijiang, Minpig and Erhualian. The continuous 9 polymorphic sites were checked by PCR-RFLP, the results indicated that Erhualian had extraordinary gene frequency, presented most significant difference by x2 test compared with Landrace, Largewhite, Neijiang and Minpig respectively, significant level compared with Meishan; and Meishan also had significant difference compared with Landrace and Minpig respectively. These results can be concluded that Taihu pigs have special genetic characteristics among pig breeds.

关键词： pig uncoupling protein gene clone PCR-RFLP.

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Tn5-mutagenesis and identification of atr operon and trpE gene responsible for indole-3-acetic acid synthesis in Azospirillum brasilense Yu62

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Progress in Natural Science:Materials International 2006年第4期16卷 366-372页

作者： XIE Baoen and CHEN Sanfeng State Key Laboratory for Agrobiotechnology, College of Biological Sciences, china agricultural university, beijing 100094, china State Key Laboratory for Agrobiotechnology College of Biological Sciences China Agricultural University Beijing 100094 China

To bring more information about synthesis of indole-3-acetic acid (IAA) from Azospirillum brasilense, a Tn5-insertion library of A. brasilense Yu62 was constructed and subjected to screening for IAA producing mutants. Two mutants with decreased IAA levels, named as A3 and A24, were isolated. The sequence analysis of loci tagged showed that the Tn5-1063a was located in the atrA gene encoding GntR family transcriptional regulator and trpE gene encoding component I of anthranilate synthase respectively. At the same time. atrB encoding phosphotransferase and atrC encoding aminotransferase were cloned downstream the atrA gene and atrA, atrB and atrC were clustered in an operon. Mutagenesis and complementation studies showed that atrA and atrC were involved in IAA synthesis. IAA levels of trpE mutant and wild-type strain could be improved by adding anthranilate into the medium.

关键词： indole-3-acetic acid (IAA) IAA mutants Azospirillum brasilense atrABC cluster trpE Tn5 transposon.

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Effect of Soil Moisture on Release of Low-Molecular Weight Organic Acids in Root Exudates and the Accu-mulation of Iron in Root Apoplasm of Peanut

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Pedosphere 2000年第4期10卷 333-338页

作者： ZHENG YI and ZHANG FUSUO (Department of Plant Nutrition, china agricultural university, beijing,100094 china) 中国农业大学北京 100094

A three-compartments rhizobox was designed and used to study the low-molecular-weight organic acids in root exudates and the root apoplastic iron of "lime-induced chlorosis" peanut grown on a calcareous soil in relation to different soil moisture conditions. Results showed that chlorosis of peanuts developed under condition of high soil moisture level (250 g kg-1), while peanuts grew well and chlorosis did not develop when soil moisture was managed to a normal level (150 g kg-1). The malic acid, maleic acid and succinic acid contents of chlorotic peanut increased by 108.723, 0.029 and 22.446 ig cm-2, respectively, compared with healthy peanuts. The content of citric acid and fumaric acid also increased in root exudates of chlorotic peanuts. On Days 28 and 42 of peanut growth, the accumulation of root apoplastic iron in chlorotic peanuts was higher than that of healthy peanuts. From Day 28 to Day 42, the mobilization percentages of chlorotic peanuts and healthy peanuts to root apoplastic iron were almost the same, being 52.4% and 52.8%, respectively, indicating that the chlorosis might be caused by the inactivation of iron within peanut plant grown on a calcareous soil under high soil moisture conditions.

关键词： apoplastic iron peanut root exudates soil moisture

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A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

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Progress in Natural Science:Materials International 2006年第8期16卷 889-892页

作者： LIU Wei, ZHANG Ying, LIU Zhaoliang, GUO Li, WANG Xiaobo, FEI Jing, FENG Jidong, ZHAO Rui, HU Xiaoxiang and LI Ning (The State Key Laboratory for Agro-Biotechnology, College of Biology, china agricultural university, beijing 100094, china College of Animal Science and Technology, china agricultural university, beijing 100094, china) The State Key Laboratory for Agro-Biotechnology College of Biology China Agricultural University Beijing 100094 China College of Animal Science and Technology China Agricultural University Beijing 100094 China

A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with HindⅢor BamHⅠthe fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480 pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10 single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identifica-

关键词： pig BAC library PCR screening system.

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Palynological characters of Glycyrrhiza,Glycyrrhizopsis,and Meristotropis(Leguminosae),with special reference to their taxonomic significance

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Journal of Systematics and Evolution 2010年第6期48卷 455-463页

作者： 1,2Lei MENG 2Xiang-Yun ZHU 1(College of Biological Sciences,china agricultural university,beijing 100094,china) 2(Institute of Botany,Chinese Academy of Sciences,beijing 100093,china) (College of Biological Sciences China Agricultural University Beijing 100094 China) (Institute of Botany Chinese Academy of Sciences Beijing 100093 China)

The pollen morphology of 11 species of the genus Glycyrrhiza *** one from each of the genera Glycyrrhizopsis Boiss.& *** Meristotropis Fisch.& *** investigated by scanning electron *** pollen morphology,the main differences between Glycyrrhizopsis and Glycyrrhiza are:Glycyrrhizopsis-pollen grains 36.63 × 40.42 μm in size,oblate spheroidal in shape;and Glycyrrhiza-pollen grains 24.47-33.18 × 23.82-31.83 μm in size,prolate spheroidal in *** and Glycyrrhiza should be recognized as two distinct genera based on palynological and morphological *** and Glycyrrhiza are similar in many important palynological and morphological characters,suggesting that the two should be *** Glycyrrhiza,two types of pollen grains,3-lobed-circular or subtriangular in polar view,are found in different species,in accordance with morphological differences in the two groups,shedding light on the classification and evolution of the genus.

关键词： exine sculpture morphological character pollen morphology taxonomic revision.

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Profile Characteristics and Potential EnvironmentalEffect of Accumulated Phosphorusin Soils ofVegetable Fields in Beliing

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Pedosphere 2002年第2期12卷 179-184页

作者： SU DECHUN, YANG FENHE and ZHANG FUSUODepartment of Plant Nutrition, china agricultural university, beijing 100094 (china) Department of Plant Nutrition China Agricultural University 北京 100094

Profile characteristics of accumulated P in 10 representativesoils of vegetable fields in suburban districts of beijing wereinvestigated. Bioavailability of the accumulated P and its potentialeffect n the environment were studied in a greenhouse pot experimentand a soil column experiment. The results showed that theconcentration of Olsen-P in the 0～20 cm soil samples of thevegetable fields ranged from 2.1 to 358.0 mg kg^-1, which was 2 to 10times higher than that of the crop field sin the suburbs of *** of the excessive phosphorus was accumulated in the topsoils.

关键词： accumulated P bioavailability environment vegetable fields

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Protein phosphorylation is involved in the water stress-induced ABA accumulation in the roots of Malus hupehensis Rehd

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Chinese Science Bulletin 2001年第10期46卷 855-858页

作者： ZHANG Dapeng, YANG Hongqiang, JIA Wensuo & HUANG Conglin1. Laboratory of Molecular Developmental Biology of Fruit Trees, china agricultural university, beijing 100094, china 2. The Key Laboratory of Plant Physiology and Biochemistry of agricultural Ministry, china agricultural university, beijing 100094, china Laboratory of Molecular Developmental Biology of Fruit Trees China Agricultural University Beijing China The Key Laboratory of Plant Physiology and Biochemistry of agricultural Ministry China Agricultural University Beijing China

Water stress-induced ABA accumulation plays a key role in the root to shoot communication and/or the cell to cell signaling under the soil stresses. The signaling of the water stress itself that leads to the accumulation, however, is less known. In this study, we subjected the roots of Malus hupehensis seedlings to water stress treatment and investigated the ABA accumulation in relation to protein phos-phorylation. Our results showed that ABA accumulation could be substantially triggered in 40 min and reached 4 folds in 100 min after treatment with 30% PEG 6000 (polyethylene glycol). The water stress treatment also led to a substantial enhancement of total kinase activity, assessed with histone-111 as substrate, in 15 min and a maximum enhancement in 30 min before it declined to initial level. The Ca2+-dependent kinase activity showed a similar, if not more sensitive, trend. When the roots were fed with labeled 32P-ATP, water stress enhanced the labeling of proteins, which showed a maximum labeling at

关键词： ABA (abscisic acid) water stress protein kinases Malus hupehensis Rehd.

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Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural Science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. College of Animal Science and Technology, china agricultural university, beijing 100094, china 2. College of Biological Science, china agricultural university, beijing 100094, china 3. College of Animal Science and Technology, Yunnan agricultural university, Kunming 650201, china) College of Animal Science and Technology China Agricultural University Beijing 100094 China College of Biological Science China Agricultural University Beijing 100094 China College of Animal Science and Technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

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