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A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum

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Science China Chemistry 2010年第4期53卷 759-767页

作者： ZHU Jun, WANG FangJun, DONG XiaoLi, YE MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry national chromatographic research and analysis center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China Key Laboratory of Separation Sciences for Analytical Chemistry Chinese Academy of Sciences Dalian China national chromatographic research and analysis center Chinese Academy of Sciences Dalian China Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian China

Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.

关键词： peptidomics label-free quantification hepatocellular carcinoma breast cancer human serum

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液相色谱荧光衍生法在糖类物质分析中的应用

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色谱 2003年第2期21卷 115-120页

作者：徐瑾张庆合张维冰王风云李彤南京理工大学江苏南京210094 中国科学院大连化学物理研究所国家色谱研究分析中心大连依利特分析仪器有限公司辽宁大连116011

介绍了目前在液相色谱荧光衍生法分析糖类物质中常用的几类荧光衍生试剂及相应的衍生方法,对近期糖类物质分离分析中的液相色谱荧光检测方法做了系统综述。引用文献60篇。

关键词：液相色谱荧光衍生法糖类物质分析荧光检测

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Development of Rapidly Quenched Soft Magnetic Materials in China

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Journal of Materials Science & Technology 2000年第2期16卷 131-136页

作者： Shaoxiong ZHOU (national Amorphous and Nanocrystalline Alloy Engineering research center, Central Iron and Steel research Institute, Beijing 100081, China) national Amorphous and Nanocrystalline Alloy Engineering research center Central Iron and Steel Research Institute Beijing 100081 China

The discovery of the first Fe-based ferromagnetic amorphous alloy in 1966 had made an impact on conventional magnetic materials because of its unique properties. Since then, a number of amorphous magnetic materials have been successfully developed and used in a wide variety of applications. A brief review of R & D activities on amorphous soft magnetic materials in China is given from the beginning to the present in a somewhat chronological order, followed by a brief introduction to their applications on electric and electronic industries. An analysis and a prospect of Chinese market of such amorphous materials are also presented.

关键词： Development of Rapidly Quenched Soft Magnetic Materials in China

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Three classes of smooth Banach submanifolds in B(E,F)

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Science China Mathematics 2007年第9期50卷 1233-1239页

作者： Ji-pu Ma Tseng Yaun Rong Functional analysis research center,Harbin Normal University,Harbin 150025,China Tseng Yaun Rong Functional analysis research center Harbin Normal University Harbin China

Let E,F be two Banach spaces, and B(E,F), Ф(E,F), SФ(E,F) and R(E,F) be the bounded linear, Fredholm, semi-Frdholm and finite rank operators from E into F, respectively. In this paper, using the continuity characteristics of generalized inverses of operators under small perturbations, we prove the following result: Let Σ be any one of the following sets: {T ∈ Ф(E,F) : IndexT = const. and dim N(T) = const.}, {T ∈ SФ(E,F) : either dim N(T) = const. < ∞ or codim R(T) = const. < ∞} and {T ∈ R(E,F) : RankT =const.< ∞}. Then Σ is a smooth submanifold of B(E,F) with the tangent space T AΣ = {B ∈ B(E,F) : BN(A) ? R(A)} for any A ∈ Σ. The result is available for the further application to Thom’s famous results on the transversility and the study of the infinite dimensional geometry.

关键词： semi-Fredholm operators smooth submanifold transversility generalized inverse 47B38 15A29 58A05

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义务教育均衡发展是实现教育公平的基石

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教育研究 2007年第2期28卷 3-11页

作者： center for Educational Policy analysis of China national Institute for Educational research 中央教育科学研究所教育政策分析中心

我国义务教育均衡发展取得了很大成就,但依然面临着诸多问题和挑战。基于问题及其原因分析,相应的政策建议是明确政府是义务教育均衡发展的全部责任方,把地方促发展和中央给补偿有机结合起来;建立以"有质量的公平增长"为导向的义务教育... 详细信息

我国义务教育均衡发展取得了很大成就,但依然面临着诸多问题和挑战。基于问题及其原因分析,相应的政策建议是明确政府是义务教育均衡发展的全部责任方,把地方促发展和中央给补偿有机结合起来;建立以"有质量的公平增长"为导向的义务教育资源配置机制;建立国家义务教育质量标准,发挥义务教育质量监测对教育公平发展的作用,并实行相应的问责制度;建立全国通用的义务教育卡,取消义务教育学校借读费,完善异地接受义务教育的保障机制;资助承担义务教育责任的民办学校。

关键词：义务教育均衡发展教育公平

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Synthesis and Characterization of Some Transition Metal Chelates of 5-(1-Hydroxy-6-Naphthylazo-3-Sodium Sulphonate) Thiobarbituric (L_1) and Barbituric (L_2) Acids

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Journal of Materials Science & Technology 1999年第3期15卷 263-266页

作者： Nabil S. Youssef and K.H.Hegab(Inorganic Chemistry Department, national research center, Dokki., Giza, Egypt) Inorganic Chemistry Department National Research Center Dokki. Giza Egypt

Some transition metal chelates of two ligands L1 and L2 were prepared and characterized by elemental analysis. The IR and 1H NMR spectra of several chelates of two ligands L1 and L2 showed the involvement of the azo group in chelation with the transition metaI ions, in most of the studied chelates, and that the two ligands L1 and L2 showed the involvement of the azo group in chelation with the transition metal ions, in most of the studied chelates, and that the two ligands L1 and L2 were coordinated either in the enol or the keto form. IR spetra also showed that Fe(III)-, Ni(II)-, Mn(II)-, VO(II)-L1 and Cu(II)-L2 chelates behaved in a bidentate manner, in contrast with the two (1:1) M:L cobalt chelates with the two ligands are *** analysis indicated the presence of three to twelve water molecules of hydration eliminated on heating up to 150℃ and one or two coordinated water molecules removed at 150~180℃.The octahedral structure is proposed fOr all the chelates, except Cu(II)-L2 and Ni(II)-L1 chelates which have square planar geometry, based on their electronic spectra

关键词： III Acids Hydroxy-6-Naphthylazo-3-Sodium Sulphonate L1 L2 Synthesis and Characterization of Some Transition Metal Chelates of 5 Thiobarbituric and Barbituric

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Numerical simulation on electromagnetic field,flow field and temperature field in semisolid slurry preparation by A-EMS

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Rare Metals 2010年第6期29卷 635-641页

作者： ZHANG Zhifeng,CHEN Xingrun,XU Jun,and SHI Likai national Engineering & Technology research center for Nonferrous Metal Matrix Composites,General research Institute for Nonferrous Metals,Beijing 100088,China national Engineering & Technology research center for Nonferrous Metal Matrix Composites General Research Institute for Nonferrous Metals Beijing China

A two-dimensional computational model coupling an annular electromagnetic stirring（A-EMS） with a macroscopic heat and fluid flow analysis in Al-alloys semisolid slurry preparation was *** dynamic evolutions of the electromagnetic field,flow field,and temperature field were presented successfully by commercial software ANSYS 10.0 with corresponding experimental verification.A horizontally rotational electromagnetic field and,thereby,a more intensive velocity field were uniformly distributed in the stirred melt even at commercial frequency,and thus,a lower temperature difference in the stirred melt and subsequent uniformly fine microstructures were obtained compared with the normal electromagnetic *** simulation results were in good agreement with experimental ones.

关键词： metal forming electromagnetic stirring numerical analysis semisolid slurry

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The relationship between sea surface temperature anomalies in the North Atlantic and summer monsoon in South Asia

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Acta Oceanologica Sinica 1991年第4期10卷 555-564页

作者： Song Jiaxi national research center for Marine Environment Forecasts, Beijing 100081, China

Having been calculated and analysed, it is found in this research paper that there exist a close lag relationship between sea surface temperature (SST) change in the north of the North Atlantic and height change at 500 hPa level in South Asia. In this paper, it is focused on the analysis of physical processes of the relation between SST and height and their effect on summer monsoon in South Asia.

关键词： over

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Comparative Study on Inorganic Composition and Crystallographic Properties of Cortical and Cancellous Bone

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Biomedical and Environmental Sciences 2010年第6期23卷 473-480页

作者： XIAO-YAN WANG,YI ZUO,DI HUANG,XIAN-DENG HOU,AND YU-BAO LI 2 research center for Nano-Biomaterials analysis and Testing,Sichuan University,Chengdu 610064,Sichuan,China research center for Nano-Biomaterials analysis and Testing Sichuan University Chengdu 610064 Sichuan China

Objective To comparatively investigate the inorganic composition and crystallographic properties of cortical and cancellous bone via thermal treatment under 700 ℃. Methods Thermogravimetric measurement, infrared spectrometer, X-ray diffraction, chemical analysis and X-ray photo-electron spectrometer were used to test the physical and chemical properties of cortical and cancellous bone at room temperature 250 ℃, 450 ℃, and 650 ℃, respectively. Results The process of heat treatment induced an extension in the a-lattice parameter and changes of the c-lattice parameter, and an increase in the crystallinity reflecting lattice rearrangement after release of lattice carbonate and possible lattice water. The mineral content in cortical and cancellous bone was 73.2wt% and 71.5wt%, respectively. For cortical bone, the weight loss was 6.7% at the temperature from 60 ℃ to 250℃, 17.4% from 250 ℃ to 450 ℃, and 2.7% from 450 ℃ to 700 ℃. While the weight loss for the cancellous bone was 5.8%, 19.9%, and 2.8 % at each temperature range, the Ca/P ratio of cortical bone was 1.69 which is higher than the 1.67 of stoichiometric HA due to the B-type CO32- substitution in apatite lattice. The CaJP ratio of cancellous bone was lower than 1.67, suggesting the presence of more calcium deficient apatite. Conclusion The collagen fibers of cortical bone were arrayed more orderly than those of cancellous bone, while their mineralized fibers ollkded similar. The minerals in both cortical and cancellous bone are composed of poorly crystallized nano-size apatite crystals with lattice carbonate and possible lattice water. The process of heat treatment induces a change of the lattice parameter, resulting in lattice rearrangement after the release of lattice carbonate and lattice water and causing an increase in crystal size and crystallinity. This finding is helpful for future biomaterial design, preparation and application.

关键词： Cortical bone Cancellous bone Bone mineral Composition Crystallographic property

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A Novel Pentadecanuclear Neodymium Cluster: Synthesis and Structure of [Nd_(15)(μ_4-tyr)_(10)(μ_3-OH)_(20)(μ_2-OH)_4(OH)_(10)(μ_2-H_2O)-(H_2O)_(10)][Na(ClO_4)_2]·61H_2O

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Chinese Chemical Letters 1998年第12期9卷 0-0,0-0页

作者： Rui Yao WANG Zhe Ming WANG Song GAO Tian Zhu JIN Zhi Da CHEN Chun Hua YAN Guang Xian XU (State Key Laboratory of Rare Earth Material Chemistry and ApplicationsPeking UniverSity, Beijing 100871)Cun Heng HE (national research center for analysis of Dr State Key Lab. Rare Earth Mat. C./A. Peking University Beijing 100871 China Natl. Res. Ctr. Anal. Drugs/M. Beijing 100050 China

The title complex was synthesized by dissolving tyrosine (tyr) in an aqueous solution ofNd(ClO4)3 at NpH5, and its crystal stfucture was determined by X-ray diffraction methods. Thecomplex is a novel pentadecanuclear with the 15 Nd3+ ions connected by μ3-OH- ions. The tyrosineligands are all in their anionic form, and each coordinates to the neodymium ions via the nitrogenatom of the amino group and the two carboxyl oxygen atoms. The molecule a barrel structure.

关键词： Neodymium tyrosine pentadecanuclear cluster

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